DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF64C and Itsn2

DIOPT Version :9

Sequence 1:	NP_001261425.1	Gene:	RhoGEF64C / 38578	FlyBaseID:	FBgn0035574	Length:	1984	Species:	Drosophila melanogaster
Sequence 2:	XP_038969030.1	Gene:	Itsn2 / 313934	RGDID:	1590890	Length:	1716	Species:	Rattus norvegicus

Alignment Length:	1680	Identity:	316/1680 - (18%)
Similarity:	561/1680 - (33%)	Gaps:	469/1680 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly   488 PTSPAIKSILKRPTTLELPGVGMSPLPL-PPVNNSGLVTPAGKSQFYIPTPTGTAATPTSSSTPM 551
            |.:|....:....:...:|.: |.|.|| |.|:.|.|             |.|||:.....|.|.
  Rat   133 PVAPITAPLSSATSGTSIPPL-MMPAPLVPSVSTSSL-------------PNGTASLIQPLSIPY 183

  Fly   552 ATSTPTPNASITATTAAAGAAITPGSRKKVQFMVE-DKIIATTATAATTDDSDAS-----AVATK 610
            ::||....:|.:......|.|    |.:|.|.::: ....:|::||:.:.:|..:     ||...
  Rat   184 SSSTLPHASSYSLMMGGFGGA----SIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQP 244

  Fly   611 TNYEY---YNRKRNAATATAAAATAASAAAASPPTAATSTDQQEATLFA-ADIDDEHNFTTSGEA 671
            :..:|   :|....:.:...:...|.:|...|     ..:..|.||::. ||||.:...     .
  Rat   245 SRLKYRQKFNSLDKSMSGYLSGFQARNALLQS-----NLSQTQLATIWTLADIDGDGQL-----K 299

  Fly   672 APPFIKPDSLHYEDVLPGPQIFDSSESSAKLSLPADGSPHVTTSPVSVLPTGTSNGVGGAGLGPG 736
            |..||.  ::|..|:             ||.     |.|...|.|..::|
  Rat   300 AEEFIL--AMHLTDM-------------AKA-----GQPLPLTLPPELVP--------------- 329

  Fly   737 PAGVSGEEAPATAAATAAATQKTTAANRDYDDDDEHNDKDESHDNKPN-DLAER------EQDRE 794
            |:...|::.. :...|..:.|||..............||.:::..:.| :|.:|      :|.||
  Rat   330 PSFRGGKQID-SINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQRE 393

  Fly   795 G------DKEQGRAGELGISRQEKKDAGGKPAASQKR---AAQTTQSRNPIRRAHSERHLS--TA 848
            .      :||:....:..:..||.|    |....:||   ..:..:.|...||...||..|  :.
  Rat   394 AERKAQKEKEEWERKQRELQEQEWK----KQLELEKRLEKQRELERQREEERRKEIERRESCISF 454

  Fly   849 PL----------------AAHGHASSACAAGRNCHSIPMTPPASGPQNVILNFKD---TMALRIK 894
            ||                .|......:|||.:..............:..:||.|:   ...:|:.
  Rat   455 PLMREREWISVYKDRKGFMAELWKPGSCAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLN 519

  Fly   895 SNR---------------------SDSRDLFQRRPTEPPPPPPPKGAGKGLEPLPHEINKSPLSP 938
            |.:                     .|.|...|.:.||             ||.|..:.:
  Rat   520 SKKKSLHLELEAVNGKHQQISGRLQDVRIRKQTQKTE-------------LEVLDKQCD------ 565

  Fly   939 QRTVVRVAPFKPQQQQVGGSGDGFTHEV-------HRLKISHSEEDADLGVELRRRPNLFLSSPG 996
                :.:...|..||::....:...:.|       .|:|........|.|:.|..:.    ||..
  Rat   566 ----LEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKK----SSEK 622

  Fly   997 EE---------DAAPKKTSILISGDDCYSTMNLSANAAQPLYQSSVVVNSHSATSVCIS------ 1046
            ||         ||..|:|:..:|..|.::......|....:.|..:.:.|      |::
  Rat   623 EELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLS------CLNNLFLLL 681

  Fly  1047 ---VSSAEEQEQELNQLNTLRSQQMGMGIAIIPIRGASEVNLQRSVSCISSTSSSSTSSSSSSSG 1108
               ..|...|:..|.||:.::..::            .|:..:| :..|........::..:..|
  Rat   682 KELRESYNTQQLALEQLHKIKRDKL------------KELERKR-LEQIQKKKLEDEAARKAKQG 733

  Fly  1109 R---GRTLIRLDYDRERKGSATSSTPINTPPVISSTPATPPPTDLPIENANPLVPHETELLKILR 1170
            :   .:..||.:.:.::|......:...|......|.|....|...:.|...|.|.|.      |
  Rat   734 KENLWKESIRKEEEEKQKRLQEEKSQDRTQEEERKTEAKQSETARALVNYRALYPFEA------R 792

  Fly  1171 NPVEAVKLNLVPHVCGLRAPTRESGKSKKIQEEEVSGTPVKDPGFIAKLLQD-----PMLGQVAE 1230
            |..| :..|              ||...::.|:.|.     :||::....|.     |.
  Rat   793 NHDE-MSFN--------------SGDIIQVDEKTVG-----EPGWLYGSFQGKFGWFPC------ 831

  Fly  1231 GLETETVAELIENSLQRLQQARRGIDMSGTKDHDDMNRLISASLQRIRLERQPATPGISATKEED 1295
                        |.::::        :|..|.......|:            |....:|||
  Rat   832 ------------NYVEKM--------LSSDKTPSPKKALL------------PPAVSLSAT---- 860

  Fly  1296 RLSNRGSISSANSFASAHNYELFDFDGGAGGNESDCYQSCSSELTASGILEEDSVSPIKDPETEL 1360
              |.......:|..|...:|:...|   :..|.:..:|. .|..|.:  :...|||||   ..:.
  Rat   861 --SAAPQPLCSNQPAPVTDYQNVSF---SNLNVNTTWQQ-KSAFTRT--VSPGSVSPI---HGQG 914

  Fly  1361 DAATRAKFYQLLVDATLAEIEH---STHMDQTLGTDSEHHYESIRHSGDPIYEE--IHEVP-PPL 1419
            .|....| .|.|...|..:..|   |.|...|:....|:.:....|.|...:.:  :..:| ..:
  Rat   915 QAVENLK-AQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEV 978

  Fly  1420 PLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGLAREESFDYGNNPKIIEEEAADTLS 1484
            ....|....|.:.||...:.:....:| |..|...:.|.|   :.:|..|....:.:::......
  Rat   979 KRGEPEALYAAVNKKPTSTAYPVGEEY-IALYSYSSVEPG---DLTFTEGEELLVTQKDGEWWTG 1039

  Fly  1485 DLDKRQA-----EERERDRDS-GQSSMSSLS-PPPHNFICGGAGKSSGSD------------VER 1530
            .:.:|..     ..|.:|::: |.:|.|..| ..|.......|..:||::            :::
  Rat  1040 SIGERTGIFPSNYVRPKDQENVGNASKSGASNKKPEIAQVTSAYAASGAEQLSLAPGQLILILKK 1104

  Fly  1531 QDSG------------------VGSETSKTSRSKYQATPTVLHLCE---------DCDGAVEMQV 1568
            ..||                  ..|.......|..:.||....:|:         :.:..:....
  Rat  1105 NSSGWWQGELQARGKKRQKGWFPASHVKLLGPSAERTTPAFHAVCQVIAMYDYIANNEDELNFSK 1169

  Fly  1569 GD-----------------NGIL------YAPLVC------RKCG-------KKRVERKE--IIT 1595
            |.                 ||:.      |..:..      :.|.       .:.:|||.  .|.
  Rat  1170 GQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPMERKRQGYIH 1234

  Fly  1596 EIVETEEKYGRDLQIILEEFCHPMLVAGLLTQEQLSAIFLNTEDLLENNQTLAERMRDALDMSLE 1660
            |::||||:|..|||:::|.|...|..:|.||:.:::.||:|.::|:.:|..|.:    ||.:..:
  Rat  1235 ELIETEERYMDDLQLVIEVFQKRMAESGFLTEAEMALIFVNWKELIMSNTKLLK----ALRVRKK 1295

  Fly  1661 QGDDDLLTVNIGRIFLDFTQMLHAFESYCVRQA-GASLLLANLEKEKELLRIFLKVSQMENAVLR 1724
            .|.:.:....:|.|.......:.|:..:|..|. ||:||....:::.:......|::  .:...:
  Rat  1296 TGGEKMPVEMMGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDADFKEFLKKLA--SDPRCK 1358

  Fly  1725 RMNLNSFLMVPVQRVTKYPLLLARLYKVTPSHLEGRELLKQAQEKIELHLNHINQEAKDVPTKLW 1789
            .|.|:|||:.|:||:|:||||:..:.:.||.:......||.|.|:.|...:.:|:..::
  Rat  1359 GMPLSSFLLKPMQRITRYPLLIRSILENTPQNHVDHSSLKLALERAEELCSQVNEGVRE------ 1417

  Fly  1790 RRISSSSPNRRASCEIDMINIKLRKMAIDVLEWNHDEVRFAMEG---RLLYTQPTDSNWKKARTI 1851
                                    |...|.|||....|:  .||   :|::...|:.
  Rat  1418 ------------------------KENSDRLEWIQAHVQ--CEGLAEQLIFNSLTNC-------- 1448

  Fly  1852 KLTPVNALLVTNGKPSANYRAEKAMSEK-----------------LNFPKHTGIREASLLLVKEK 1899
             |.|  ..|:.:||      ..|..|.|                 ..|...:|..:   |...:.
  Rat  1449 -LGP--RKLLYSGK------LYKTKSNKELHGFLFNDFLLLTYLVRQFAASSGFEK---LFSSKS 1501

  Fly  1900 CGRYTLIREPLYLDRCVVCSEADWDDYFEVQEISSKDTFIFKAEDGVRTKQWYTQ 1954
            ..::.:.:.|::|:..:|....|......|..||..|.......|.:..:..:.|
  Rat  1502 SAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQ 1556

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	55/186 (30%)
Itsn2	XP_038969030.1	EH	15..107	CDD:197477
		KLF3_N	106..>185	CDD:410554	17/65 (26%)
		EH	238..317	CDD:197477	21/108 (19%)
		PTZ00121	<347..797	CDD:173412	90/506 (18%)
		SH3_Intersectin2_1	780..836	CDD:212921	17/99 (17%)
		SH3_Intersectin2_2	921..972	CDD:212923	11/51 (22%)
		SH3_Intersectin2_3	1004..1055	CDD:212925	8/54 (15%)
		SH3_Intersectin2_4	1076..1134	CDD:212927	6/57 (11%)
		SH3_Intersectin2_5	1149..1202	CDD:212929	5/52 (10%)
		RhoGEF	1233..1413	CDD:214619	55/185 (30%)
		PH_13	1431..1574	CDD:406942	26/148 (18%)
		C2_Intersectin	1574..1708	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	88	1.000	Domainoid score	I7756
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.910