DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF64C and Itsn2

DIOPT Version :9

Sequence 1:	NP_001261425.1	Gene:	RhoGEF64C / 38578	FlyBaseID:	FBgn0035574	Length:	1984	Species:	Drosophila melanogaster
Sequence 2:	NP_001185897.1	Gene:	Itsn2 / 20403	MGIID:	1338049	Length:	1685	Species:	Mus musculus

Alignment Length:	1846	Identity:	325/1846 - (17%)
Similarity:	579/1846 - (31%)	Gaps:	600/1846 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   375 NVAAIAGQRISLGDKSGDSSDP------GDSYERIIMVSGGESTASNGPRRPPRNKKADEPAEMP 433
            |:.||..:..:..||..|:..|      ||......:.||                       :|
Mouse    14 NMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSG-----------------------LP 55

  Fly   434 AAAGAAGAAGAAATSPPAAAVTAATAVKPKAVTLAKVEKIEKDNESAYTV--------LTVTPTS 490
            |              |..|.:.|          |:.:.|..|.::..:::        |......
Mouse    56 A--------------PVLAEIWA----------LSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLP 96

  Fly   491 PAIKSILKRPTTLELP------GVGMSP-----LPLPPVNNSGLVTP--AGKSQFYIP------- 535
            ..:..|:|:|.... |      |:|..|     .|||||  :.:.||  :..|...||
Mouse    97 VVLPPIMKQPPMFS-PLISARFGMGSMPNLSIHQPLPPV--APIATPLSSATSGTSIPPLMMPAP 158

  Fly   536 ---------TPTGTAATPTSSSTPMATSTPTPNASITATTAAAGAAITPGSRKKVQFMVE-DKII 590
                     .|.|||:.....|.|.::||....:|.:......|.|    |.:|.|.::: ....
Mouse   159 LVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGA----SIQKAQSLIDLGSSS 219

  Fly   591 ATTATAATTDDSDAS-----AVATKTNYEY---YNRKRNAATATAAAATAASAAAASPPTAATST 647
            :|::||:.:.:|..:     ||...:..:|   :|......:...:...|.:|...|     ..:
Mouse   220 STSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQS-----NLS 279

  Fly   648 DQQEATLFA-ADIDDEHNFTTSGEAAPPFIKPDSLHYEDVLPGPQIFDSSESSAKLSLPADGSPH 711
            ..|.||::. ||||.:...     .|..||.  ::|..|:             ||.     |.|.
Mouse   280 QTQLATIWTLADIDGDGQL-----KAEEFIL--AMHLTDM-------------AKA-----GQPL 319

  Fly   712 VTTSPVSVLPTGTSNG--VGGA-GLGPGPAGVSGEEAPATAAATAAATQKTT------------- 760
            ..|.|..::|.....|  |... |..|.......||.......|....:|..
Mouse   320 PLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQ 384

  Fly   761 --AANRDYDDDDEHNDKDESHDNKPNDLAEREQDREGDKEQGRAGELGISRQ---------EKKD 814
              ...:..:.:.:...:.|..:.|..:|.|:|..::.:.|:....:..:.||         |:::
Mouse   385 VLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERRE 449

  Fly   815 AGGKPAASQKR-----------AAQTTQSRNPIRRAHSERHLSTAPLAAHGHASSACAAGRNCHS 868
            |..:....|:|           .:|.|:.:..|.|..|.:......|.|
Mouse   450 AAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEA---------------- 498

  Fly   869 IPMTPPASGPQNVILNFKDTMALRIKSNRSDSRDLFQRRPTEPPPPPPPKGAGKGLEPLPHEINK 933
                  .:|....|......:.:|.::.:::...|.::...|.          ..::.|..|:.:
Mouse   499 ------VNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEI----------MEIKQLQQELKE 547

  Fly   934 SPLSPQRTVVRVAPFKPQQQQVGGSGDGFTHEVHRLKISHSEEDADLGVELRRRPNLFLSSPGEE 998
            .    |..::.:.|.|....:             |:|........|.|:.|..:.    ||..||
Mouse   548 Y----QNKLIYLVPEKQLLNE-------------RIKNMQLSNTPDSGISLLHKK----SSEKEE 591

  Fly   999 ---------DAAPKKTSILISGDDCYSTMNLSANAAQPLYQSSVVVNSHSATSVCIS-------- 1046
                     ||..|:|:..:|..|.::......:....:.|..:.:.|      |::
Mouse   592 LCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLS------CLNNLFLLLKE 650

  Fly  1047 -VSSAEEQEQELNQLNTLRSQQMGMGIAIIPIRGASEVNLQRSVSCISSTSSSSTSSSSSSSGR- 1109
             ..|...|:..|.||:.::..::            .|:..:| :..|........::..:..|:
Mouse   651 LRESYNTQQLALEQLHKIKRDKL------------KEIERKR-LEQIQKKKLEDEAARKAKQGKE 702

  Fly  1110 --GRTLIRLDYDRERKGSATSSTPINTPPVISSTPATPPPTDLPIENANPLVPHET--------- 1163
              .|..||.:.:.::|......:...|........|....|...:.|...|.|.|.
Mouse   703 NLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFS 767

  Fly  1164 --ELLKILR----------------------NPVEAV---------KLNLVPHVCGLRAPTRESG 1195
              :::::..                      |.||.|         |..|:|....|.|.:..|.
Mouse   768 SGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQ 832

  Fly  1196 KSKKIQ----------------EEEVSGTPVKDPGFIAKLLQDPMLGQVAEGLETE--------- 1235
            ....:.                :::.:.|....||.::.:...   ||..|.|:.:
Mouse   833 PPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQ---GQAVENLKAQALCSWTAKK 894

  Fly  1236 ------------TVAELIEN---------------SLQRL---QQARRGIDMSGTKDHDDMNRLI 1270
                        ||.|..||               |..:|   .:.:||          :...|.
Mouse   895 ENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRG----------EPEALY 949

  Fly  1271 SASLQRIRLERQPATPGISATKEE-DRLSNRGSISSAN-SFASAHNYELFDFDG----GAGGNES 1329
            :|..::......|.|.......|: ..|.:..|:...: :|.......:...||    |:.|..
Mouse   950 AAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGER- 1013

  Fly  1330 DCYQSCSSELTASGILEEDSVSPIKDPE----TELDAATRAKFYQLLVDATLAEIEHSTHMDQTL 1390
                        :||...:.|.| ||.|    .....|:..|          .||...|......
Mouse  1014 ------------TGIFPSNYVRP-KDQENFGNASKSGASNKK----------PEIAQVTSAYAAS 1055

  Fly  1391 GTDSEHHYESIRHSGDPIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDA 1455
            ||                 |::...|..|.|         :.||.....::|           :.
Mouse  1056 GT-----------------EQLSLAPGQLIL---------ILKKNTSGWWQG-----------EL 1083

  Fly  1456 KERGLAREESFDYGNNPKIIEEEAADTLSDL----------DKRQAEERERDRDSGQ--SSMSSL 1508
            :.||..|::.:...::.|::...:..|:...          |.....|.|.:...||  :.|:..
Mouse  1084 QARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKD 1148

  Fly  1509 SPP-------------PHNFICGGAGKSSGSDVERQDSGVGSETSKTSRSKYQATPTVLHLCEDC 1560
            .|.             |.|::    ..::.||..:|                        .|.|.
Mouse  1149 DPDWWQGETNGLTGLFPSNYV----KMTTDSDPSQQ------------------------WCADL 1185

  Fly  1561 DGAVEMQVGDNGILYAPLVCRKCGKKRVERKEIITEIVETEEKYGRDLQIILEEFCHPMLVAGLL 1625
            .....||..:.                 :|:..|.|:::|||:|..|||:::|.|...|..:|.|
Mouse  1186 QALDTMQPTER-----------------KRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFL 1233

  Fly  1626 TQEQLSAIFLNTEDLLENNQTLAERMRDALDMSLEQGDDDLLTVNIGRIFLDFTQMLHAFESYCV 1690
            |:..::.||:|.::|:.:|..|..    ||.:..:.|.:.:....||.|.......:.|:..:|.
Mouse  1234 TEADMALIFVNWKELIMSNTKLLR----ALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCS 1294

  Fly  1691 RQA-GASLLLANLEKEKELLRIFLKVSQMENAVLRRMNLNSFLMVPVQRVTKYPLLLARLYKVTP 1754
            .|. ||:||....:::.:......|::  .:...:.|.|:|||:.|:||:|:||||:..:.:.||
Mouse  1295 CQLNGATLLQQKTDEDTDFKEFLKKLA--SDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTP 1357

  Fly  1755 -SHLEGRELLKQAQEKIELHLNHINQEAKDVPTKLWRRISSSSPNRRASCEIDMINIKLRKMAID 1818
             ||:: ...||.|.|:.|...:.:|:..::                              |...|
Mouse  1358 QSHVD-HSSLKLALERAEELCSQVNEGVRE------------------------------KENSD 1391

  Fly  1819 VLEWNHDEVRFAMEG---RLLYTQPTDSNWKKARTIKLTPVNALLVTNGKPSANYRAEKAMSEKL 1880
            .|||....|:  .||   :|::...|               |.|     .|.....:.|....|.
Mouse  1392 RLEWIQAHVQ--CEGLAEQLIFNSLT---------------NCL-----GPRKLLHSGKLYKTKS 1434

  Fly  1881 NFPKHTGIREASLLLV-----------------KEKCGRYTLIREPLYLDRCVVCSEADWDDYFE 1928
            |...|..:....|||.                 .:...::.:.:.|::|:..:|....|......
Mouse  1435 NKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEP 1499

  Fly  1929 VQEISSKDTFIFKAEDGVRTKQWYTQ 1954
            |..||..|.......|.:..:..:.|
Mouse  1500 VFHISHIDRVYTLRTDNINERTAWVQ 1525

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	57/187 (30%)
Itsn2	NP_001185897.1	EH	15..107	CDD:197477	21/138 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..242		5/21 (24%)
		EH	238..317	CDD:197477	21/108 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	335..382		8/46 (17%)
		Smc	350..>747	CDD:224117	67/468 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	716..742		3/25 (12%)
		SH3_Intersectin2_1	747..803	CDD:212921	6/55 (11%)
		INTAP	806..883	CDD:374674	12/79 (15%)
		SH3_Intersectin2_2	883..934	CDD:212923	6/50 (12%)
		SH3_Intersectin2_3	973..1024	CDD:212925	10/63 (16%)
		SH3_Intersectin2_4	1045..1103	CDD:212927	15/94 (16%)
		SH3_Intersectin2_5	1118..1171	CDD:212929	9/56 (16%)
		RhoGEF	1202..1381	CDD:366202	57/185 (31%)
		PH_13	1400..1554	CDD:374702	25/148 (17%)
		C2_Intersectin	1543..1677	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	82	1.000	Domainoid score	I8425
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.810