Protein Alignment nAChRbeta1 and acr-25

DIOPT Version :10

• Sequence 1: NP_523927.2
• Gene: nAChRbeta1 / 38545
• FlyBaseID: FBgn0000038
• Length: 521
• Species: Drosophila melanogaster
• Sequence 2: NP_001023570.2
• Gene: acr-25 / 3565563
• WormBaseID: WBGene00043066
• Length: 544
• Species: Caenorhabditis elegans
• Alignment Length: 569
• Identity: 170/569 - (29%)
• Similarity: 280/569 - (49%)
• Gaps: 104/569 - (18%)

  Fly     8 WL---LCSILVLVAFSLVSASEDEERLVRDLFRGYNKLIRPVQNMTQKVGVRFGLAFVQLINVNE 69
            ||   ||.:|.|....:...||.:  |..:|...|..|.||::|.::.|.|:.||...|:::|:|
 Worm     5 WLILKLCLLLALGRNGITQKSEGD--LYTELLEDYEPLERPIENSSEAVMVKMGLVLQQIVDVDE 67

  Fly    70 KNQIMKSNVWLRLVWYDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLI 134
            |||::..|.||:..|.||.|:|...||||:..||....::|.||::::|:||..::.||.||:|:
 Worm    68 KNQVVDVNAWLKFSWVDYSLKWKPEDYGGVTDLRFRKGQLWTPDVLMYNSADPQFDSRYASNLLV 132

  Fly   135 YPTGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSLALYNNKNFVDLSDYWKS 199
            ||.|.|.|:||.:|:.||.|.|.:||||.|.|.:|||||||:|.:::|.:  :.|..|:|:|.::
 Worm   133 YPNGLVNWMPPGLYRLSCKIQVVWFPFDVQECFLKFGSWTFDGTKLNLEI--DDNGFDISNYMQN 195

  Fly   200 GTWDIIEVPAYLNVYEGDSNHPTETDITFYIIIRRKTLFYTVNLILPTVLISFLCVLVFYLPAEA 264
            |.|.:.......|:...........|:.|..:|||:.|:|..|||||.:||:.|.::.|..|.:|
 Worm   196 GEWTLEGTTVKRNIQYYQCCPEPYYDLVFTFVIRRRALYYAFNLILPCILITMLTLVGFTFPPDA 260

  Fly   265 GEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIINWNFRGPR 329
            |||::|.|:|:||:.:|...|:::.||||..:|.::.:...........:..|.:.:|::.|..:
 Worm   261 GEKMSLQITIMLSICIFQNYVAELSPPTSEAVPFLSAFFAVCLFTCACCVTATTLALNFHHRTGK 325

  Fly   330 THRMPMYIRSIFLHYLPAFLFMKRP---------------------RKTRL---RWMMEMPGMSM 370
            :|:|....|.:.|.::|..|.|:||                     |:|||   |....:..:::
 Worm   326 SHQMNNTFRLLMLEWIPWLLMMRRPGYVARCGKIVKQDDSEDEFEERQTRLDQQRIATIISQITV 390

  Fly   371 PAH-PHPSYGSPAELPKHISAIGGKQSKMEVMELSDLHHPNCKINRKVNSGGELG---LGDGCRR 431
            .:: |.|::      |:.::.:.                       :|||.||..   ..|...|
 Worm   391 ESNKPTPTF------PRRVTIVD-----------------------EVNSDGEAEEPLPPDEVIR 426

  Fly   432 ESESSDSILLSPEASKATEA------------------------VEFIAE---------HLRNED 463
            |.:...:.:..|.|.::|..                        ||.||:         ||...:
 Worm   427 EEDDDVASMDDPPAWRSTTTTSQPPQSPAHLWWNATNRMGNRVPVEQIAQLLVLQQVHGHLSEIN 491

  Fly   464 LYIQTRE-------DWKYVAMVIDRLQLYIFFIVTTAGTVGILMDAPHI 505
            .:|:.||       |||:.|:|:||:.:.||.......||.:....|:|
 Worm   492 KHIREREKSKKIEDDWKFSALVVDRICMVIFTSFLFGSTVALFASVPNI 540

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRbeta1	NP_523927.2	LIC	8..500	CDD:455710	168/562 (30%)
		LGIC_ECD_nAChR_proto_beta-like	27..236	CDD:349833	81/208 (39%)
		Cys-loop	152..166	CDD:349833	8/13 (62%)
acr-25	NP_001023570.2	LGIC_ECD_nAChR	51..231	CDD:349798	73/181 (40%)
		Cys-loop	150..164	CDD:349798	8/13 (62%)
		LGIC_TM_cation	233..>322	CDD:349853	30/88 (34%)
		TM1 helix	233..257	CDD:349853	11/23 (48%)
		Neur_chan_memb	239..534	CDD:460753	76/323 (24%)
		TM2 helix	265..286	CDD:349853	7/20 (35%)
		TM3 helix	297..319	CDD:349853	1/21 (5%)