Protein Alignment nAChRbeta1 and lgc-4

DIOPT Version :10

• Sequence 1: NP_523927.2
• Gene: nAChRbeta1 / 38545
• FlyBaseID: FBgn0000038
• Length: 521
• Species: Drosophila melanogaster
• Sequence 2: NP_509556.4
• Gene: lgc-4 / 181155
• WormBaseID: WBGene00017580
• Length: 635
• Species: Caenorhabditis elegans
• Alignment Length: 552
• Identity: 161/552 - (29%)
• Similarity: 264/552 - (47%)
• Gaps: 97/552 - (17%)

  Fly    28 EERLVRDLF--RGYNKLIRPVQNMTQKVGVRFGLAFVQLINVNEKNQIMKSNVWLRLVWYDYQLQ 90
            ||.|.|.|.  ..|.|.:||..:.:|...|.||....|::.::|:||.:.:..||.:.|.|.:|.
 Worm   111 EEALYRSLLDHTVYEKDVRPCIHHSQPTNVTFGFLLNQIVEMDERNQALTTRSWLNINWMDPRLS 175

  Fly    91 WDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKS-NVLIYPTGEVLWVPPAIYQSSCTI 154
            |:|:.:..|..:.:|..::|||||:|.|||...|.....| :|::...|.|.|:..|:::|||.|
 Worm   176 WNESLWSEIKAIYIPHARIWKPDIILVNNAIREYYASLVSTDVMVTSDGNVTWLFSALFRSSCPI 240

  Fly   155 DVTYFPFDQQTCIMKFGSWTFNGDQVSLALYNNKNFVDLSDYWKSGTWDIIEVPAYLNVYEGDSN 219
            .|.|:|||.|.|.:||.||:.:..:::|.|..:|.  |||.|..:..:|::::.|...|.    .
 Worm   241 RVRYYPFDDQQCDLKFASWSHDITEINLGLNTDKG--DLSSYMNNSEFDLVDMTAVREVV----T 299

  Fly   220 HPTETDITFYIII-----RRKTLFYTVNLILPTVLISFLCVLVFYLPAEAGEKVTLGISILLSLV 279
            .|::|:..:.||:     .|:.|||..|.|:|.||||.:.||.|.:|.|.|||:.:.|:.|||:.
 Worm   300 FPSDTNSDWPIIVIRIHMHRRPLFYVFNHIVPCVLISSMAVLGFLMPPETGEKINMIITTLLSMG 364

  Fly   280 VFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIINWNFRGP--RTHRMPMYIRSIFL 342
            |:|..:::.:||||..:|||..|.:.:.:|..::..|.||.:|.:..|.  :...:|.:::...|
 Worm   365 VYLQSITESIPPTSEGVPLIGMYYVSSLLMVCLATCVNVITLNMHRNGAANQGRHVPAWMQKWIL 429

  Fly   343 HYLPAFLFM--KRP-----------------RKTRLRWMMEMPGMSMPAHPHPSYGSPAELPKHI 388
            .||..|:.|  :.|                 |.:.||.:..:..||                 ::
 Worm   430 GYLATFMRMSIREPDSIALLKASQSKKSTIRRSSILRDLKRVKNMS-----------------NV 477

  Fly   389 SAIGGKQSKMEVMELSD-LHH------PNC-KINRKVNSGGELGLGDGCRRESESSDSILLS--- 442
            .|...:|:.....|..| |.|      .:| ..:.|..:|..:       ||..:|:|..|.
 Worm   478 RAKSKEQNANRECECMDPLVHIYAESIMSCLAADTKPMNGSTI-------REDFASESTFLGRVV 535

  Fly   443 -----PEASKATEAV--EF--------------IAEH-LRNEDLYIQTREDWKY--VAMVIDRLQ 483
                 |..|.::.:|  ||              :.:| :|.|.|..::|..|::  :|.|:|||
 Worm   536 SDGIMPRISASSNSVLTEFETRFRRILKRVYRSLQQHEIREEILDERSRIQWQWQQLASVVDRL- 599

  Fly   484 LYIFFIVTTAGTVGILMDAPHIFEYVDQDRII 515
            |...|...|..|:..|:..|  ..|.|.|.::
 Worm   600 LLCLFCTATLFTIICLLIVP--VAYRDNDSML 629

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRbeta1	NP_523927.2	LIC	8..500	CDD:455710	156/535 (29%)
		LGIC_ECD_nAChR_proto_beta-like	27..236	CDD:349833	72/215 (33%)
		Cys-loop	152..166	CDD:349833	8/13 (62%)
lgc-4	NP_509556.4	LGIC_ECD_nAChR	138..320	CDD:349798	61/187 (33%)
		Cys-loop	238..252	CDD:349798	8/13 (62%)
		LGIC_TM_cation	322..>409	CDD:349853	37/86 (43%)
		TM1 helix	322..346	CDD:349853	13/23 (57%)
		TM2 helix	354..375	CDD:349853	6/20 (30%)
		TM3 helix	386..408	CDD:349853	5/21 (24%)