Protein Alignment nAChRbeta1 and nAChRalpha2

DIOPT Version :10

• Sequence 1: NP_523927.2
• Gene: nAChRbeta1 / 38545
• FlyBaseID: FBgn0000038
• Length: 521
• Species: Drosophila melanogaster
• Sequence 2: XP_311921.3
• Gene: nAChRalpha2 / 1272988
• VectorBaseID: AGAMI1_000572
• Length: 569
• Species: Anopheles gambiae
• Alignment Length: 549
• Identity: 227/549 - (41%)
• Similarity: 323/549 - (58%)
• Gaps: 52/549 - (9%)

  Fly     8 WLLCSILVL-VAFSLVSASEDEERLVRDLFRGYNKLIRPVQNMTQKVGVRFGLAFVQLINVNEKN 71
            |:...|::| :....|.|:.|.:||..||...||:|||||.|.|..|.|:.||...|||::|.|:
Mosquito    15 WMTLGIVLLCMVIGSVWANPDAKRLYDDLLSNYNRLIRPVSNNTDTVLVKLGLRLSQLIDLNLKD 79

  Fly    72 QIMKSNVWLRLVWYDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLIYP 136
            ||:.:|:||...|.|::.:||.|:|||:..|.:|.:.:|.|||||:|||||.|.|...:..:::.
Mosquito    80 QILTTNIWLEHEWQDHKFKWDPAEYGGVTELYVPSEHIWLPDIVLYNNADGEYVVTTLTKAILHY 144

  Fly   137 TGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSL----ALYNNKNFV----DL 193
            ||:|:|.||||::|||.|||.|||||||||.|||||||::|:|:.|    .|.|:.|.|    ||
Mosquito   145 TGKVIWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGNQIDLKHKNQLNNSDNMVKIGIDL 209

  Fly   194 SDYWKSGTWDIIEVPAYLNVYEGDSNHP----TETDITFYIIIRRKTLFYTVNLILPTVLISFLC 254
            .:|:.|..|||:.|||..:    :..:|    ...||.|.|.:||||||||||||:|.|.||:|.
Mosquito   210 REYYPSVEWDILGVPAERH----EKYYPCCAEPYPDIFFNITLRRKTLFYTVNLIIPCVGISYLS 270

  Fly   255 VLVFYLPAEAGEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVI 319
            ||||||||::|||:.|.||||||..:|.||:|:|:|.|||.|||:.||||||.|:..:|:::|:|
Mosquito   271 VLVFYLPADSGEKIALCISILLSQTMFFLLISEIIPSTSLALPLLGKYLLFTMILVGLSVVITII 335

  Fly   320 IINWNFRGPRTHRMPMYIRSIFLHYLPAFLFMKRPR-------KTRLRWMMEMP----GMSMPAH 373
            |:|.::|.|.||:|..::|..|:..||..|.|:.|.       ..::.:.:::.    |.::.||
Mosquito   336 ILNIHYRKPSTHKMAPWVRKFFILRLPKLLLMRVPNDLLKELAANKINYGIKISKSKFGAALAAH 400

  Fly   374 PHPSYGSPAELPKHISAIGGKQSKMEVMELSDLHHPNCKINR-----KVNSGGELGLG--DGCRR 431
            ......|....|..|..:.|:......:      |.....||     ....||..|||  :|...
Mosquito   401 SQMQPNSGGSSPDSIRHMQGRPGGCNGL------HSTTATNRFSGLVGALGGGLSGLGGYNGLPS 459

  Fly   432 ESESSDSIL--LSP------EASKATEAVEFIAEHLRNEDLYIQTREDWKYVAMVIDRLQLYIFF 488
            .....|..|  ::|      |..||...|.||..|::.:|.:....:||.:||||:|||.|::|.
Mosquito   460 VMSGLDESLSDVAPRKKYPFELEKAIHNVMFIQHHMQRQDEFNAEDQDWGFVAMVLDRLFLWLFT 524

  Fly   489 IVTTAGTVGILMDAPHIFEYVDQDRIIEI 517
            |.:..||..||.:||.::   |..:.|:|
Mosquito   525 IASLFGTFAILCEAPSLY---DDTKPIDI 550

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRbeta1	NP_523927.2	LIC	8..500	CDD:455710	221/530 (42%)
		LGIC_ECD_nAChR_proto_beta-like	27..236	CDD:349833	105/220 (48%)
		Cys-loop	152..166	CDD:349833	11/13 (85%)
nAChRalpha2	XP_311921.3	LIC	15..535	CDD:455710	220/529 (42%)
		LGIC_ECD_nAChR_proto_alpha-like	35..254	CDD:349832	107/222 (48%)
		Cys-loop	160..174	CDD:349832	11/13 (85%)