Protein Alignment kst and Sptbn4

DIOPT Version :10

• Sequence 1: NP_001097492.2
• Gene: kst / 38418
• FlyBaseID: FBgn0004167
• Length: 4337
• Species: Drosophila melanogaster
• Sequence 2: XP_006540500.1
• Gene: Sptbn4 / 80297
• MGIID: 1890574
• Length: 2580
• Species: Mus musculus
• Alignment Length: 2748
• Identity: 739/2748 - (26%)
• Similarity: 1226/2748 - (44%)
• Gaps: 424/2748 - (15%)

  Fly    33 PGGYSALQTPTPSNRN--SANMTQRD-------------GIIKFENERIKTLQEERLHIQKKTFT 82
            ||....::.|..||.|  ||.....|             ....||..|||.|.:||..:||||||
Mouse    24 PGEVDNMEGPAVSNNNNPSARWESPDRGWDREPPAAANAAASLFECSRIKALADEREAVQKKTFT 88

  Fly    83 KWMNSFLIKAKMEVEDLFTDLADGIKLLKLLEIISSEKLGKPNSGRMRVHKIENVNKSLAFL-HT 146
            ||:||.|.:....:.||:.||.||..|.:|||::|.|:|.:|..||||:|.:|||:|:|.|| ..
Mouse    89 KWVNSHLARVGCHIGDLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQ 153

  Fly   147 KVRLESIGAEDIVDGNPRLILGLIWTIILRFQIQEIEIDVDEENESSEKRSAKDALLLWCQRKTH 211
            :|.||::|:.||||||.||.|||:||||||||||.|:|:.::..|:   ||||||||||||.||.
Mouse   154 RVHLENVGSHDIVDGNHRLTLGLVWTIILRFQIQVIKIETEDNRET---RSAKDALLLWCQMKTA 215

  Fly   212 GYPGVNITDFTNSWRSGLGFNALIHSHRPDLFEYSTIVNSKNSNLDNLNHAFDTAANELGIPSLL 276
            |||.|||.:||.|||.||.||||||.|||||.:.|.:..| |:|. ||..||.||...||:..||
Mouse   216 GYPEVNIQNFTTSWRDGLAFNALIHRHRPDLVDLSKLTKS-NANY-NLQRAFRTAEQHLGLARLL 278

  Fly   277 DAEDIDSARPDEKSILTYVASYYHTFARMKNEQKSGKRIANIVGQLMDADRKKMQYEGLTTNLLS 341
            |.||::...||||||:|||.|:||.|::||.....||||..::.|:::.|:...:||.|...||:
Mouse   279 DPEDVNMEAPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLA 343

  Fly   342 WIRQKTLELEQRDLPNSLEGIQRELLAFKEYRTIEKPPKYKERSEIEALYFTINTLLKALNQPPY 406
            ||.:....:..:...|||.|:|::|.||..|.|:|||.|::|:..:|.|.|:|.:.|:|.|:..:
Mouse   344 WIHRTVDLISNQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLF 408

  Fly   407 NPQDGQLVNDIEKAWQILEYAEHHREVALRDELLRQEKLEQLNYKFEKKSVLREGYLKEMIQVLS 471
            .|::|..:.||:|||..||.|||.||.|||.||:||||||.|..:|:.|..:||.:|.|..:::|
Mouse   409 VPREGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVS 473

  Fly   472 DPRY---LRQVDATLKKHEAISADILARVERFNDLTAMAEELDRENYHGKERVRRREQEVMAKWR 533
            ...:   |..|:|.:||||||.|||.|..||...:..:|:.|..|.|:...||..:...|:.:|.
Mouse   474 QDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWA 538

  Fly   534 QLLELLENQRLNLSQMSNLMNLLREIASTTEAVRELQQQFASEDVGPHLLGVEELLQAHSLQELQ 598
            .|..|:..:|..|.|...|..:.:|:....:.:.|:|.|..|.:.|.||:..::|||.|.|.|..
Mouse   539 LLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGD 603

  Fly   599 VNTYGETLKRFNRQALPY---KSSEHKDAALLAQRLADLEEAYSELLRRSAARRARLEEARNFHH 660
            :....|.::..|..||.:   :..:..|..::..|:..:....|||..::|.|||.||.:|:...
Mouse   604 IAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWA 668

  Fly   661 FMEDYDNEESWLVDKQRICK----------TGITAKDLRAVLSLQQKHKALEDEIKSRKPKSGQM 715
            .:::.:..|||..||:|:.:          |...|.||.:...|..:||.|:.|:..|:....|.
Mouse   669 LLQELEEAESWARDKERLLEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQA 733

  Fly   716 STAGKRLI--------GEQHPRSSEIQSRIDSLAEHWQALEALVELRRRQLEDAAEAYQFYTDAN 772
            ...|:.|.        |.:....:.:..|..|....||.||.....|.|:|::|...:||..|.:
Mouse   734 LRRGEELAAAGGSVGPGAEPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLD 798

  Fly   773 EAESWLNEKIALVNSRDYGNDEPSAQALLQRHRDLQGELNAYSGDILNLNQQADKLIKAGICTLE 837
            ....||.:...|..:.|:|:||.|::.|.::||.|.||:.|:.|.:..|.:|...|..|
Mouse   799 GLLDWLRDAYRLAAAGDFGHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGA------ 857

  Fly   838 LSAAEPELPEVEQEEWVNETRLVPKEVWEDEWVEKLEHKKVTETKMLPHVKSLFPFEGQGMKMDK 902
             |.|.|
Mouse   858 -SGAGP----------------------------------------------------------- 862

  Fly   903 GEVMLLKSKTNDDWWCVRKDNGVEGFVPANYVREVEPRPVACIVPKAEKVKSLQKVKKTILVRQV 967
                                     .|.|..||.||          ||
Mouse   863 -------------------------LVVALQVRVVE----------AE----------------- 875

  Fly   968 VPVKRIKPVSVAPKPLVQRRTSTQSINENADSVEKRQQRINQTYDELQEMAQKRHALLEDSIHLF 1032
                                                     |.:.|:.|:|..|...|.|::.::
Mouse   876 -----------------------------------------QLFAEVTEVAALRRQWLRDALAVY 899

  Fly  1033 GFYRECDDFEKWMKEKERMIKS----DEGEGVDNAKRKFEKFITDLSAASKRVEEIDGAVDTFRR 1093
            ..:.|....|.|:.|||:.:.:    |..:.|:..:.:||....::::...||.:::..|.....
Mouse   900 RMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQEMNSLMGRVLDVNQTVQELVE 964

  Fly  1094 QGHSQLDKIIARQRQIHQIWQRLNNAKAQREKSLEGASSVELFNRTCDEAKVWMSEKMLQLDTAV 1158
            .||...|::.:.|..::..|.|:.....||::.:.....||.......|.:..:.||...:::|.
Mouse   965 GGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAP 1029

  Fly  1159 ITPDLRTVQALQRRHQNLERELAPVEDKVNRVTYLGNSVKNAYPAEKDNVNARQQEVQDMWQQVQ 1223
                 |...|||.|...||..|..:|.:...:......:...:||:...::...:|:...|..:.
Mouse  1030 -----RAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALA 1089

  Fly  1224 QRGSDLRNRIESEVGQQVFNNSAKVLLAWIDSVKDQLNADES--ARDVETANNLLKKHNDLGDDI 1286
            .........:.:....|.|.......|.|:...::...|.|.  .|.:|.|:.||.:|..|.:::
Mouse  1090 GAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEV 1154

  Fly  1287 RAHDTEFVEVIQLGKQL--SDGKPNMAETVAVIERLKAEQDAIHR--G-WAEKQKWLLQCVDLQM 1346
            ...:.::..::...:.|  |:| ..:...:|:.|.|...:...|:  | |.|:::.|:|....|:
Mouse  1155 DQREEDYARIVAASEALLASEG-AELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQL 1218

  Fly  1347 FNREADKIDATTKSHEAFLEYNNLGASLDEVEAILKRHLDFEKSLMAQDKILKGFSDNADKLISN 1411
            |.|:..:..|..::.|..|....|..:::.||..:|||.||..::....:.::.....|:.|:..
Mouse  1219 FLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAESLLRQ 1283

  Fly  1412 DHYDSKYIGDRRNQVLGKRKAVKDRAFERKRLLQASKDFHKFAAEADDLKVWLQDKTRIAGDENY 1476
            .:...:...:...::|.|.:..:.||.:..:.|........|..:..:|..|:.:|..:|.|...
Mouse  1284 GNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTR 1348

  Fly  1477 RDLSNLPRKLQKHQAFERELRANEGQLRNVTKDGQALVQAGNRVPE----VESRVADLNKRWKDL 1537
            .|...|.::..:||||..||..|:..|..:.::||.|:|   ..||    |..::.::.:.|.:|
Mouse  1349 EDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQ---EKPELAASVRKKLGEIRQCWAEL 1410

  Fly  1538 LTLSEDKGRKLEQAASQREHNRSLEDAKKKVDELDSALRSGDVGNDLRSCKDLINKQQILESEIT 1602
            .:.::.|.|:|.:|:...:..:|..:..|::..::|.|:..|.|.||.:....:.|.|.:||::.
Mouse  1411 ESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVE 1475

  Fly  1603 IWDQKVAELVSTGDDMAHGGHFNAQN----IEAGTKEL--------QQRFKDLRDPTQRRRAKLE 1655
            .|.::|.||             .||.    :|..:|||        .:|...|.:|.|.||..|.
Mouse  1476 EWCREVGEL-------------QAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLL 1527

  Fly  1656 ESLNYHKFVFELDSEFQWINEHLPAAKSNELGQNLHQAQSLHKKHKKLEAEIKGHQPMINKALVA 1720
            .|...|:...:||.|..|:.|.||.|...|.|..|...|...||::.|..||:.|.|.:.:.|..
Mouse  1528 ASKELHQVAHDLDDELAWVQERLPLAMQTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLER 1592

  Fly  1721 GQSLISQQHPEREQVESLCQQLEQAWQDLERHCGERSRKLDMSLKAQQYLFDAGEIESWLGERNN 1785
            ...|.|.:.||.|.|....:||:.||..|......|.:.||.:.:.:||.||..|:|:||||:..
Mouse  1593 AGVLASLRSPEAEAVRRGQEQLQSAWTGLREAAERRQQTLDAAFQVEQYYFDVAEVEAWLGEQEL 1657

  Fly  1786 VLRSTEYGRDRDSAAKLLTKHKTIELELDTYSGIVTEMGHSCAAMVAANHPDSKVLAAKQQLIEK 1850
            ::.|.:.|:|..|..:||.||..:|..::.|...:.::...|.|::...||||:.::.:|..:::
Mouse  1658 LMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDR 1722

  Fly  1851 MLKSLHKLASQRQGRLMESLYKHEYFLESDEVEQWIREQEQAASSEDYGQDFEHLQLLQNKFDDL 1915
            :..:|.:|..:|:..|.:..:.::...:.||:|.||.|:|..|.|.:.||||||:.:||.||.:.
Mouse  1723 LYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEF 1787

  Fly  1916 KHRV-EVGADRVDQCELLAKKLIDSESPYANEVEKRQEQLRTSWENLLQLLNQREQKLHAAGEIH 1979
            .... ..|.:|:.....:..:||:.....|..:.:.::.|..:|..||:|:..|.|.|.|:.|:|
Mouse  1788 ASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELH 1852

  Fly  1980 RFHRDVAEALFRIQDKNAALSQELG--RDLNSALALLRKHEGFENDLVALEAQLQVLVEDSVRLQ 2042
            :|..|..|...:|::|...|.:...  ....||.::.|....||:||..|.:|::.|.|.:.:|:
Mouse  1853 KFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLR 1917

  Fly  2043 AKYP-SNASAIAQQQDKVVAAWNDLKERSTARGDRLAASSDLQTFLTDVRDIVSWSSNLRAALQA 2106
            ..|. .:|.|||.::.:|:..|.:|..........:::::|...|.:..||::||...:...:.|
Mouse  1918 TVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGA 1982

  Fly  2107 EEHVSDAAGATALKIQHDAIYGEIEAREDKFRYLNELSDSMVQTGHYAAADVEEKCAAMLDERQK 2171
            .:...|.:....|...|..:..|:|||..:.....||..|::......|.:::.:...:...:::
Mouse  1983 ADKPRDVSSVEVLMNYHQGLKTELEARVPELATCQELGRSLLLNKSAMADEIQAQLDKLGSRKEE 2047

  Fly  2172 LHAAWNKKKIMLEQKIDLFCFLRDAKQIDNLSSSQQAALSSSDFGQTVEDVQNKIRKHDEFERLI 2236
            :...|::....|:|.:::..|.::|...|...::|:..|.|.:.|.:|::|:..||:|:.|.:..
Mouse  2048 VSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAA 2112

  Fly  2237 QTQEEKVSLLQE-------------------HGRK--------------LIEQRHYDSANIQTIL 2268
            ...||:.|.|:.                   .|||              |:....||
Mouse  2113 AAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYD-------- 2169

  Fly  2269 QGV--LARR------QKVKDLCAVRRYKLEDALLYAKFVRDCAEAKYWINEKQKKLEADAASYAE 2325
            :|:  ||||      .:|:......|.:|:...|..:..|        :.|...|:|..|.:.|.
Mouse  2170 RGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDR--------LPETSGKVEPAAPTAAA 2226

  Fly  2326 VTNLD--------EKIKKLQKHQ--AFQAEVAANQGRIQEIQDTGVILLSKQHESS----PEIKR 2376
            :...|        |.::...:.|  |.:||....:.| .|.|::    :.:..|::    ||.:.
Mouse  2227 LDTTDTPGTPAATELVRPRSERQELADRAEELPRRRR-SERQES----VDQPEETARRRRPERQE 2286

  Fly  2377 AIE------IVLEAWQGLLAELEQRGRGLEEAQDSLE---FNSQLDKIEAWIRDKEMMVQASDTG 2432
            :.:      :.|..::.:....|:|.|.:|..:.|.:   ...:|.|.:|.:.|....:|..:.|
Mouse  2287 SADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAG 2351

  Fly  2433 -------------RDLEHCNALMRKLDDVDSDMRVDDQRVK------------------------ 2460
                         |:|.. ..|...|:..:...|.|..|.:
Mouse  2352 PGIPAGVPSLPQPRELPP-GRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSR 2415

  Fly  2461 -----------------HINQLADKLINQAQVPADTQSVDKRRKDFNYNWRQL-----QGALNAY 2503
                             |..|....|:.:       :.:|..||..|.:|..|     :|.|..|
Mouse  2416 SAPAQGGSAPAPPPPPTHTVQHEGFLLRK-------RELDANRKSSNRSWVSLYCVLSKGELGFY 2473

  Fly  2504 RALLGGAN----------EIHVFNRDVDDTADRIAEKSLAMSSTDTGRDLAAVEALIRREEALER 2558
            :...|.|:          .:|....:|  .:|...:|.:....|..|.:. .::|  :.||.:..
Mouse  2474 KDSKGPASGGTHGGEPLLSLHKATSEV--ASDYKKKKHVFKLQTQDGSEF-LLQA--KDEEEMNG 2533

  Fly  2559 DMSAVKQKIDQHETAAEF 2576
            .:.||...:.:|...|.:
Mouse  2534 WLEAVANSVAEHAEIARW 2551

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
kst	NP_001097492.2	CH_beta_spectrin_rpt1	61..176	CDD:409042	64/115 (56%)
		CH_beta_spectrin_rpt2	196..302	CDD:409043	67/105 (64%)
		SPEC	450..640	CDD:238103	56/195 (29%)
		SPEC	658..832	CDD:238103	52/191 (27%)
		SH3	885..935	CDD:214620	2/49 (4%)
		SPEC	1030..1236	CDD:238103	40/209 (19%)
		SPEC	1134..1341	CDD:238103	42/213 (20%)
		SPEC	1343..1551	CDD:238103	49/211 (23%)
		SPEC	1449..1658	CDD:238103	58/224 (26%)
		SPEC	1555..1764	CDD:238103	65/220 (30%)
		SPEC	1765..1975	CDD:238103	63/210 (30%)
		SPEC	1873..2080	CDD:238103	61/210 (29%)
		SPEC	1979..2185	CDD:238103	47/208 (23%)
		SPEC	2188..2402	CDD:238103	55/274 (20%)
		SPEC	2407..2618	CDD:238103	40/242 (17%)
		SPEC	2514..2725	CDD:238103	13/63 (21%)
		SPEC	2726..2934	CDD:238103
		SPEC	2935..3145	CDD:238103
		SPEC	3042..3251	CDD:238103
		SPEC	3149..3357	CDD:238103
		SPEC	3359..3567	CDD:238103
		SPEC	3465..3680	CDD:238103
		PH_beta_spectrin	3801..3904	CDD:269975
Sptbn4	XP_006540500.1	CH_SF	40..183	CDD:469584	67/142 (47%)
		CH_SPTBN4_rpt2	185..314	CDD:409171	76/133 (57%)
		SPEC	332..555	CDD:238103	90/222 (41%)
		SPEC	450..664	CDD:238103	66/213 (31%)
		SPEC	790..1002	CDD:238103	62/370 (17%)
		SPEC	1106..1210	CDD:197544	23/104 (22%)
		SPEC	1216..1424	CDD:238103	49/210 (23%)
		SPEC	1322..1529	CDD:238103	58/222 (26%)
		SPEC	1531..1742	CDD:238103	66/210 (31%)
		SPEC	1637..1849	CDD:238103	64/211 (30%)
		Spectrin	1849..1948	CDD:395348	28/98 (29%)
		SPEC	1958..>2125	CDD:238103	38/166 (23%)
		PH_beta_spectrin	2437..2541	CDD:269975	23/115 (20%)