Protein Alignment kst and alpha-Spec

DIOPT Version :10

• Sequence 1: NP_001097492.2
• Gene: kst / 38418
• FlyBaseID: FBgn0004167
• Length: 4337
• Species: Drosophila melanogaster
• Sequence 2: NP_001261286.1
• Gene: alpha-Spec / 38231
• FlyBaseID: FBgn0250789
• Length: 2457
• Species: Drosophila melanogaster
• Alignment Length: 2995
• Identity: 712/2995 - (23%)
• Similarity: 1241/2995 - (41%)
• Gaps: 752/2995 - (25%)

  Fly   732 EIQSRIDSLAEHWQALEALVELRRRQLEDAAEAYQFYTDANEAESWLNEKIALVNSRDYGNDEPS 796
            :||.|.:.:...:...:.....:|.:|||:.....|..||:|.|||::||:...:...| .|..:
  Fly    17 DIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEKLQAASEESY-RDPTN 80

  Fly   797 AQALLQRHRDLQGELNAYSGDILNLNQQADKLIKAGICTLELSAAEPELPEVEQEEWVNETRLVP 861
            .||.:|:|:..:.|::|:|..|::|:....::|.
  Fly    81 LQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMIN------------------------------- 114

  Fly   862 KEVWEDEWVEKLEHKKVTETKMLPHVKSLFPFEGQGMKMDKGEVMLLKSKTNDDWWCVRKDNGVE 926
                                                                             
  Fly   115 ----------------------------------------------------------------- 114

  Fly   927 GFVPANYVREVEPRPVACIVPKAEKVKSLQKVKKTILVRQVVPVKRIKPVSVAPKPLVQRRTSTQ 991
                                                                      |:..:::
  Fly   115 ----------------------------------------------------------QQHFASE 121

  Fly   992 SINENADSVEKRQQRINQTYDELQEMAQKRHALLEDSIHLFGFYRECDDFEKWMKEKERMIKSDE 1056
            ||....|.:.|..:.:      |..:|:| ...|:.::.|..|.|:|::...|:|:||..:.:||
  Fly   122 SIQVRLDELHKLWELL------LSRLAEK-GLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADE 179

  Fly  1057 G----EGVDNAKRKFEKFITDLSAASKRVEEIDGAVDTFRRQGHSQLDKIIARQRQIHQIWQRLN 1117
            .    |.|:..:|||::|..|:::...||.|::...|...:.||.:.|.|..|:.::::.||||.
  Fly   180 FGQDLEHVEVLQRKFDEFQKDMASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLK 244

  Fly  1118 NAKAQREKSLEGASSVELFNRTCDEAKVWMSEKMLQLDTAVITPDLRTVQALQRRHQNLERELAP 1182
            .....|::.|.||..::.|||..||...|::||.:.|.:.....||.:||||||:|:.:||:||.
  Fly   245 QLAIVRQEKLFGAHEIQRFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAA 309

  Fly  1183 VEDKVNRVTYLGNSVKNAYPAEKDNVNARQQEVQDMWQQVQQRGSDLRNRIESEVGQQVFNNSAK 1247
            :||||:.:......:.:.:....|.:..:|.|:.:.||.:..:..:.:.:::.......|....:
  Fly   310 LEDKVSTLGAEAQRLCSIHADHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFR 374

  Fly  1248 VLLAWIDSVKDQLNADESARDVETANNLLKKHNDLGDDIRAHDTEFVEVIQLGKQLSD----GKP 1308
            .|::||:.:|..::|||.|:||..|..||::|.:...:|.|.:..|....:.|::|.:    ...
  Fly   375 DLVSWINGMKAIISADELAKDVAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAA 439

  Fly  1309 NMAETVAVIERLKAEQDAIHRGWAEKQKWLLQCVDLQMFNREADKIDATTKSHEAFLEYNNLGAS 1373
            .:.|.:|.:|..|:...::   |.:::....||:|||:|.|:.::.|......||||...:||.|
  Fly   440 EIQEKLAALENDKSSLLSL---WEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDS 501

  Fly  1374 LDEVEAILKRHLDFEKSLMAQDKILKGFSDNADKLISNDHYDSKYIGDRRNQVLGKRKAVKDRAF 1438
            ||.|||::|:|.||||||.||::.:|.....|.|||...||.:..:..||..:|.:|.|:::::.
  Fly   502 LDSVEALIKKHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSS 566

  Fly  1439 ERKRLLQASKDFHKFAAEADDLKVWLQDKTRIAGDENYRDLSNLPRKLQKHQAFERELRANEGQL 1503
            :|::||:.|..:.:|..:.|:.|.|:.:|.:.|.|::|.|.:||..|:||||.||.||.||:.::
  Fly   567 KRRQLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRI 631

  Fly  1504 RNVTKDGQALVQAGN-RVPEVESRVADLNKRWKDLLTLSEDKGRKLEQAASQREHNRSLEDAKKK 1567
            .::|..|..|::..: ...::.:|:.::...|:.|:..|:.||.||.:|..|::.||::||.:..
  Fly   632 EDITNVGTELIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELW 696

  Fly  1568 VDELDSALRSGDVGNDLRSCKDLINKQQILESEITIWDQKVAELVSTGDDMAHGGHFNAQNIEAG 1632
            :.|::..|.|.|.|.||.|.::|..|..:||:::.....::..:....:.....|||:|.||...
  Fly   697 LSEIEGQLLSEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNK 761

  Fly  1633 TKELQQRFKDLRDPTQRRRAKLEESLNYHKFVFELDSEFQWINEHLPAAKSNELGQNLHQAQSLH 1697
            ...|..|:..|..|...|:..|.:||...:...:|:.|..||.|..|.|.|...|::|...|:|.
  Fly   762 EGNLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLI 826

  Fly  1698 KKHKKLEAEIKGHQPMINKALVAGQSLISQQHPEREQVESLCQQLEQAWQDLERHCGERSRKLDM 1762
            |||:.:.|||..|:..:...:.:|::::..|....:.:....:.|::.|..|:....:|.:.||.
  Fly   827 KKHQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDD 891

  Fly  1763 SLKAQQYLFDAGEIESWLGERNNVLRSTEYGRDRDSAAKLLTKHKTIELELDTYSGIVTEMGHSC 1827
            ||:|.||..||.|.|||:.|:..:...::||:|.||:..||.||:.:..:|:.:
  Fly   892 SLQAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAF----------- 945

  Fly  1828 AAMVAANHPDSKVLAAKQQLIEKMLKSLHKLASQRQGRLMESLYKHEYFLESDEVEQWIREQEQA 1892
                                                |..:::|                  |||
  Fly   946 ------------------------------------GNTIQAL------------------QEQ- 955

  Fly  1893 ASSEDYGQDFEHLQLLQNKFDDLKHRVEVGADRVDQCELLAKKLIDSESPYANEVEKRQEQLRTS 1957
                                                    ||.....|:|..:...|
  Fly   956 ----------------------------------------AKNCRQQETPVVDITGK-------- 972

  Fly  1958 WENLLQLLNQREQKLHAAGEIHRFHRDVAEALFRIQDKNAALSQELGRDLNSALALLRKHEGFEN 2022
                                      :...||:...:|:.
  Fly   973 --------------------------ECVVALYDYTEKSP------------------------- 986

  Fly  2023 DLVALEAQLQVLVEDSVRLQAKYPSNASAIAQQQDKVVAAWNDLKERSTARGDRLAASSDLQTFL 2087
                                                        :|.|..:||.|       |.|
  Fly   987 --------------------------------------------REVSMKKGDVL-------TLL 1000

  Fly  2088 TDVRDIVSWSSNLRAALQAEEHVSDAAGATALKIQHDAIYGEIEAREDKFRYLNELSDSMVQTGH 2152
                     :||                                   :|..:..|::|   :.|.
  Fly  1001 ---------NSN-----------------------------------NKDWWKVEVND---RQGF 1018

  Fly  2153 YAAADVEEKCAAMLDERQKLHAAWNKKKIMLEQKIDLFCFLRDAKQIDNLSSSQQAALSSSDFGQ 2217
            ..||.:                                      |:||       |.||:|.
  Fly  1019 VPAAYI--------------------------------------KKID-------AGLSASQ--- 1035

  Fly  2218 TVEDVQNKIRKHDEFERLIQTQEEKVSLLQEHGRKLIEQRHYDSANIQTILQGVLARRQKVKDLC 2282
                 ||.:..|...:|..|...:..:||                        .|||
  Fly  1036 -----QNLVDNHSIAKRQNQINSQYDNLL------------------------ALAR-------- 1063

  Fly  2283 AVRRYKLEDALLYAKFVRDCAEAKYWINEKQKKLEADAASYAEVTNLDEKIKKLQKHQAFQAEVA 2347
                                        |:|.||.
  Fly  1064 ----------------------------ERQNKLN------------------------------ 1070

  Fly  2348 ANQGRIQEIQDTGVILLSKQHESSPEIKRAIEIVLEAWQGLLAELEQRGRGLEEAQDSLEFNSQL 2412
                                     |..:|..:|.||     |:|.|
  Fly  1071 -------------------------ETVKAYVLVREA-----ADLAQ------------------ 1087

  Fly  2413 DKIEAWIRDKEMMVQASD-TGRDLEHCNALMRKLDDVDSDMRVDDQRVKHINQLADKLINQAQVP 2476
                 ||||||...|.:| .|.|||....|.:|.||.:.|::.::.|:.::|::|.:|.:..|..
  Fly  1088 -----WIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLGQTE 1147

  Fly  2477 ADTQSVDKRRKDFNYNWRQLQGALNAYRA-LLGGANEIHVFNRDVDDTADRIAEKSLAMSSTDTG 2540
            | ...:..:.:|.|..|..|| .|.|.:| .||.|:|:..|:||:|:|.|.||||:.|:::.|.|
  Fly  1148 A-ALKIQTQMQDLNEKWNNLQ-TLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLG 1210

  Fly  2541 RDLAAVEALIRREEALERDMSAVKQKIDQHETAAEFLIKKYPERGAQHIERKLEELHKSWGNLQA 2605
            :||.:|:.|.|:.|.:|||::|::.||.|.:..|..|::.:|:. |:....|.:|:::.|..:..
  Fly  1211 KDLRSVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHPDT-AEQTYAKQKEINEMWDQIIT 1274

  Fly  2606 LSVKRQSILNEAYLAHKFVSDVKELELWVNDMIKKMNNTQSPSTINDCETQLELHQ--------- 2661
            .|..|:..|.::|...:|:||.::|..|:|.|:..:.:.:..:.:...|..:|.||
  Fly  1275 KSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQARRAEIGFT 1339

  Fly  2662 -----------------------ERKVEIEGRQEAFAGLKQQGEQLSKRPQQQQPDNVRKYLLVL 2703
                                   |.:.||:.|...|...:|.|.:|.:......|: :::.:..|
  Fly  1340 LGISSAPGAAASTSSIASPSGDEEHRTEIDARAGTFGAFEQFGNELLQANHYASPE-IKEKIEDL 1403

  Fly  2704 EELHQTLNEAWSERARDLTEAHQLQLFKAQVEQVEIWLANKEAFLNNDDLGDSYTAVERLLKKHD 2768
            .:..:.|.:||:||...|.:...|||:....|..|.|::.:|||||.||..::...||.|:|||:
  Fly  1404 AKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHE 1468

  Fly  2769 EFEKLL--HADHVDTLQKFANSILEGEPKDADLIREKLAYILRRKQKLLELSEERKQRLTQSHQL 2831
            :|:|.:  |...:..||..|:.::......::|:.||...:|.|.:.|.|...|::.||.....|
  Fly  1469 DFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEKRKQVLERWRHLKEGLIEKRSRLGDEQTL 1533

  Fly  2832 QEFLRSLYEIDRWLVQKLQVALDENYREPSNLQSKIQKHAAFDAELLSNSPRVQSVIHEGERLIR 2896
            |:|.|...||:.|:.:|||:|.:|:|::|:|:|||.|||.||:|||.:|:.|:|||:..|..||.
  Fly  1534 QQFSRDADEIENWIAEKLQLATEESYKDPANIQSKHQKHQAFEAELAANADRIQSVLAMGGNLID 1598

  Fly  2897 GDHFAKDEIAQQVQL--LEGDWLKLKGASQTKKDKLQQAYDALAFNRSVDEFNNWMDEVELQLSS 2959
            ....:..|.|.|.:|  :...|..|...:..|..||::|.....:..:|.:.:.|:.|||..|::
  Fly  1599 KKQCSGSEDAVQKRLTQIADQWEYLTHKTTEKSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTT 1663

  Fly  2960 EDYGKDLAAVSNLLKKHERLEAD-VAHHGELADQLKQKDEQFFQAEHFLRHEIHERATVSIRRYN 3023
            ||.|||||:|.||:|||:.:||| |||...:.|...|.| ...::..|....|.|:......||.
  Fly  1664 EDSGKDLASVQNLMKKHQLVEADIVAHEDRIKDMNNQAD-SLVESGQFDTAGIQEKRQSINERYE 1727

  Fly  3024 TLHEPLGIRRENLEDSLSLQQFLRDAEDELQWLAEKQLVAGSQDLGTSLLSVQGLQKKHNSLEAE 3088
            .:......|:..|.::|:|.||.||..||..|:.||:|:.||.|.|..|..||.|:|||..||||
  Fly  1728 RICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAE 1792

  Fly  3089 LTSQEPLIQALLQRGQQMIRDNHFASEQLQYKSELLQKQLVQLRDLAAIRRLRLLDAVESQLFYV 3153
            |.|.||.|||:.:.|::::..::....:::.:.:.|.:...:|::|||.|..:|.:::..|.|..
  Fly  1793 LGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQAWAELKNLAATRGQKLDESLTYQQFLA 1857

  Fly  3154 EANEADAWMREKRPVLSSSDYGRDEVSVQGHQKKLEVLQRELTAFKPSIEKVAKLATGLIERNHF 3218
            :..|.:||:.||:.:||..|||....:|||..||.:..:.:.||.|.....:....:.|:|..:.
  Fly  1858 QVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNH 1922

  Fly  3219 DSSNIAEKNAQVGQEYEDLLRLAKERESRLGECKKLFEYLRETEELHEWVGDQMAVTASEDYGED 3283
            ...:||::..|:..:.::|..||..|:..|.:.....:::.:.:.:..|:.|:.....|:::|.|
  Fly  1923 HGESIAQRCQQLRLKLDNLSALAARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRD 1987

  Fly  3284 VEHVEQLILAFESFVSNLNANEAR-VEACLERGDRLIQENNPYRSSIKSKRDETKQLWEELKDLV 3347
            :..|:.|:...|:|.:.|||.|.. :.......|:||..::....:|..:..:....|::|:|..
  Fly  1988 LSTVQTLLTKQETFDAGLNAFEQEGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDAS 2052

  Fly  3348 HARQDALAGAKQVHVYDRVADETIQLINEKDASLIS---EDYGQDL---------ESIQALGRKH 3400
            :.|:|.|...::  .:.::  |.:.|...|.||..:   |:..:||         |.|:||...|
  Fly  2053 NTRKDRLLAMQE--QFRQI--EELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAH 2113

  Fly  3401 QVFESELVGIQGQVDSVLAEAAKLGEIYPDAKEHIEVKRDETVEAWTDLKEKTAARKNKLSQAEQ 3465
            ..|::.|...:....::.|...|:.........:.....:...|.|.:|::....|..:|::..:
  Fly  2114 AQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAK 2178

  Fly  3466 LQSYFDEYRDLIA--------WINE----MLA-KITAPELANSVAGAELLLASTKDHDTEIRTRD 3517
            .|...|:.|...|        |:.|    ||. ......:.......|..|.:.:...||:|.|.
  Fly  2179 RQEENDKLRKEFAKHANLFHQWLTETRYYMLGYNRQGTSMMEGSGSLEQQLEALRVKATEVRARR 2243

  Fly  3518 ETFAKFAANG----QQLIKEKHFLAHEVEDKIKVLQARHELLKHTLNKRREIYELNLDTQLFLKD 3578
            ....|....|    :.||.:..:..|.                 |:...::..:|:   ||.::.
  Fly  2244 VDLKKIEELGALLEEHLILDNRYTEHS-----------------TVGLAQQWDQLD---QLSMRM 2288

  Fly  3579 AEILEQWISSR------EPQLKDTKLGDSIPQVEDLLRRHEDFEKTVAAQEEKFQAIKR-----I 3632
            ...|||.|.:|      |..||:.          .::.:|.|.:|:.....::|::..|     :
  Fly  2289 QHNLEQQIQARNHSGVSEDSLKEF----------SMMFKHFDKDKSGKLNHQEFKSCLRALGYDL 2343

  Fly  3633 TMLEQ 3637
            .|:|:
  Fly  2344 PMVEE 2348

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
kst	NP_001097492.2	CH_beta_spectrin_rpt1	61..176	CDD:409042
		CH_beta_spectrin_rpt2	196..302	CDD:409043
		SPEC	450..640	CDD:238103
		SPEC	658..832	CDD:238103	28/99 (28%)
		SH3	885..935	CDD:214620	0/49 (0%)
		SPEC	1030..1236	CDD:238103	65/209 (31%)
		SPEC	1134..1341	CDD:238103	56/210 (27%)
		SPEC	1343..1551	CDD:238103	77/208 (37%)
		SPEC	1449..1658	CDD:238103	63/209 (30%)
		SPEC	1555..1764	CDD:238103	57/208 (27%)
		SPEC	1765..1975	CDD:238103	30/209 (14%)
		SPEC	1873..2080	CDD:238103	16/206 (8%)
		SPEC	1979..2185	CDD:238103	18/205 (9%)
		SPEC	2188..2402	CDD:238103	29/213 (14%)
		SPEC	2407..2618	CDD:238103	71/212 (33%)
		SPEC	2514..2725	CDD:238103	64/242 (26%)
		SPEC	2726..2934	CDD:238103	78/211 (37%)
		SPEC	2935..3145	CDD:238103	77/210 (37%)
		SPEC	3042..3251	CDD:238103	71/208 (34%)
		SPEC	3149..3357	CDD:238103	53/208 (25%)
		SPEC	3359..3567	CDD:238103	42/236 (18%)
		SPEC	3465..3680	CDD:238103	38/201 (19%)
		PH_beta_spectrin	3801..3904	CDD:269975
alpha-Spec	NP_001261286.1	SPEC	48..258	CDD:238103	63/371 (17%)
		SPEC	154..364	CDD:238103	65/209 (31%)
		SPEC	261..470	CDD:238103	57/211 (27%)
		SPEC	471..680	CDD:238103	77/208 (37%)
		SPEC	682..893	CDD:238103	58/210 (28%)
		Spectrin	893..>961	CDD:395348	28/173 (16%)
		SH3_Alpha_Spectrin	974..1026	CDD:212742	18/212 (8%)
		SPEC	1074..1287	CDD:238103	78/243 (32%)
		SPEC	1182..1425	CDD:238103	65/244 (27%)
		SPEC	1426..1638	CDD:238103	78/211 (37%)
		SPEC	1533..1744	CDD:238103	79/211 (37%)
		SPEC	1646..1850	CDD:238103	77/204 (38%)
		SPEC	1852..2062	CDD:238103	53/209 (25%)
		SPEC	1958..2176	CDD:238103	45/221 (20%)
		SPEC	2072..2296	CDD:238103	47/243 (19%)
		EFh	2311..2379	CDD:238008	7/48 (15%)
		EFhand_Ca_insen	2386..2456	CDD:430177