Protein Alignment kst and Sptbn4

DIOPT Version :10

• Sequence 1: NP_001097492.2
• Gene: kst / 38418
• FlyBaseID: FBgn0004167
• Length: 4337
• Species: Drosophila melanogaster
• Sequence 2: XP_008757363.1
• Gene: Sptbn4 / 308458
• RGDID: 1304682
• Length: 2609
• Species: Rattus norvegicus
• Alignment Length: 2747
• Identity: 737/2747 - (26%)
• Similarity: 1223/2747 - (44%)
• Gaps: 422/2747 - (15%)

  Fly    33 PGGYSALQTPTPSNRN--SANMTQRD-------------GIIKFENERIKTLQEERLHIQKKTFT 82
            ||....::.|..||.|  :|.....|             ....||..|||.|.:||..:||||||
  Rat    53 PGEVDNMEGPAASNNNNPAARWESPDRGWDREPPAAANAAASLFECSRIKALADEREAVQKKTFT 117

  Fly    83 KWMNSFLIKAKMEVEDLFTDLADGIKLLKLLEIISSEKLGKPNSGRMRVHKIENVNKSLAFL-HT 146
            ||:||.|.:....:.||:.||.||..|.:|||::|.|:|.:|..||||:|.:|||:|:|.|| ..
  Rat   118 KWVNSHLARVGCHIGDLYADLRDGFVLTRLLEVLSGEQLPRPTRGRMRIHSLENVDKALQFLKEQ 182

  Fly   147 KVRLESIGAEDIVDGNPRLILGLIWTIILRFQIQEIEIDVDEENESSEKRSAKDALLLWCQRKTH 211
            :|.||::|:.||||||.||.|||:||||||||||.|:|:.::..|:   ||||||||||||.||.
  Rat   183 RVHLENVGSHDIVDGNHRLTLGLVWTIILRFQIQVIKIETEDNRET---RSAKDALLLWCQMKTA 244

  Fly   212 GYPGVNITDFTNSWRSGLGFNALIHSHRPDLFEYSTIVNSKNSNLDNLNHAFDTAANELGIPSLL 276
            |||.|||.:||.|||.||.||||||.|||||.:.|.:..| |:|. ||..||.||...||:..||
  Rat   245 GYPEVNIQNFTTSWRDGLAFNALIHRHRPDLVDLSKLTKS-NANY-NLQRAFRTAEQHLGLARLL 307

  Fly   277 DAEDIDSARPDEKSILTYVASYYHTFARMKNEQKSGKRIANIVGQLMDADRKKMQYEGLTTNLLS 341
            |.||::...||||||:|||.|:||.|::||.....||||..::.|:::.|:...:||.|...||:
  Rat   308 DPEDVNMEAPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQVLEVDKIIERYEELAAELLA 372

  Fly   342 WIRQKTLELEQRDLPNSLEGIQRELLAFKEYRTIEKPPKYKERSEIEALYFTINTLLKALNQPPY 406
            ||.:....:..:...|||.|:|::|.||..|.|:|||.|::|:..:|.|.|:|.:.|:|.|:..:
  Rat   373 WIHRTVDLISNQKFANSLSGVQQQLQAFTAYCTLEKPVKFQEKGNLEVLLFSIQSKLRAHNRRLF 437

  Fly   407 NPQDGQLVNDIEKAWQILEYAEHHREVALRDELLRQEKLEQLNYKFEKKSVLREGYLKEMIQVLS 471
            .|::|..:.||:|||..||.|||.||.|||.||:||||||.|..:|:.|..:||.:|.|..:::|
  Rat   438 VPREGCGIWDIDKAWGELEKAEHEREAALRAELIRQEKLELLAQRFDHKVAMRESWLNENQRLVS 502

  Fly   472 DPRY---LRQVDATLKKHEAISADILARVERFNDLTAMAEELDRENYHGKERVRRREQEVMAKWR 533
            ...:   |..|:|.:||||||.|||.|..||...:..:|:.|..|.|:...||..:...|:.:|.
  Rat   503 QDNFGYELPAVEAAMKKHEAIEADIAAYEERVQGVAELAQALAAEGYYDARRVAAQRDSVLRQWA 567

  Fly   534 QLLELLENQRLNLSQMSNLMNLLREIASTTEAVRELQQQFASEDVGPHLLGVEELLQAHSLQELQ 598
            .|..|:..:|..|.|...|..:.:|:....:.:.|:|.|..|.:.|.||:..::|||.|.|.|..
  Rat   568 LLTGLVGARRTRLEQNLALQKVFQEMVYMVDWMEEMQTQLLSRECGQHLVEADDLLQKHGLLEGD 632

  Fly   599 VNTYGETLKRFNRQALPY---KSSEHKDAALLAQRLADLEEAYSELLRRSAARRARLEEARNFHH 660
            :....|.::..|..||.:   :..:..|..::..|:..:....|||..::|.|||.||.:|:...
  Rat   633 IAAQSERVEALNAAALRFSQLQGYQPCDPQVICNRVNHVHGCLSELQEQAARRRAELEASRSLWA 697

  Fly   661 FMEDYDNEESWLVDKQRICK----------TGITAKDLRAVLSLQQKHKALEDEIKSRKPKSGQM 715
            .:::.:..|||..||:|:.:          |...|.||.:...|..:||.|:.|:..|:....|.
  Rat   698 LLQELEEAESWARDKERLLEATSGSGGAAGTAGGAHDLSSTARLLAQHKILQGELGGRRALLQQA 762

  Fly   716 STAGKRLI--------GEQHPRSSEIQSRIDSLAEHWQALEALVELRRRQLEDAAEAYQFYTDAN 772
            ...|:.|.        |.:....:.:..|..|....||.||.....|.|:|::|...:||..|.:
  Rat   763 LRRGEELAAAGGSVGPGAEPLHLAGLAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLD 827

  Fly   773 EAESWLNEKIALVNSRDYGNDEPSAQALLQRHRDLQGELNAYSGDILNLNQQADKLIKAGICTLE 837
            ....||.:...|..:.|:|:||.|::.|.::||.|.||:.|:.|.:..|.:|...|..|
  Rat   828 GLLDWLRDAYRLAAAGDFGHDEASSRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGA------ 886

  Fly   838 LSAAEPELPEVEQEEWVNETRLVPKEVWEDEWVEKLEHKKVTETKMLPHVKSLFPFEGQGMKMDK 902
             |.|.|
  Rat   887 -SGAGP----------------------------------------------------------- 891

  Fly   903 GEVMLLKSKTNDDWWCVRKDNGVEGFVPANYVREVEPRPVACIVPKAEKVKSLQKVKKTILVRQV 967
                                     .|.|..||.||          ||
  Rat   892 -------------------------LVVALQVRVVE----------AE----------------- 904

  Fly   968 VPVKRIKPVSVAPKPLVQRRTSTQSINENADSVEKRQQRINQTYDELQEMAQKRHALLEDSIHLF 1032
                                                     |.:.|:.|:|..|...|.|::.::
  Rat   905 -----------------------------------------QLFAEVTEVAALRRQWLRDALAVY 928

  Fly  1033 GFYRECDDFEKWMKEKERMIKS----DEGEGVDNAKRKFEKFITDLSAASKRVEEIDGAVDTFRR 1093
            ..:.|....|.|:.|||:.:.:    |..:.|:..:.:||....::::...||.:::..|.....
  Rat   929 RMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQEMNSLMGRVLDVNQTVQELVE 993

  Fly  1094 QGHSQLDKIIARQRQIHQIWQRLNNAKAQREKSLEGASSVELFNRTCDEAKVWMSEKMLQLDTAV 1158
            .||...|::.:.|..::..|.|:.....||::.:.....||.......|.:..:.||...:::|.
  Rat   994 GGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVLEVAEVRAQVREKRRAVESAP 1058

  Fly  1159 ITPDLRTVQALQRRHQNLERELAPVEDKVNRVTYLGNSVKNAYPAEKDNVNARQQEVQDMWQQVQ 1223
                 |...|||.|...||..|..:|.:...:......:...:||:...::...:|:...|..:.
  Rat  1059 -----RAGGALQWRLSGLEAALQALEPRQAALLEEAALLAERFPAQATRLHQGAEELGAEWGALA 1118

  Fly  1224 QRGSDLRNRIESEVGQQVFNNSAKVLLAWIDSVKDQLNADES--ARDVETANNLLKKHNDLGDDI 1286
            .........:.:....|.|.......|.|:...::...|.|.  .|.:|.|:.||.:|..|.:::
  Rat  1119 GAAQACGEAVAAAGRLQRFLRDLDTFLDWLVRAQEAAGAVEGPLPRSLEEADGLLARHAALKEEV 1183

  Fly  1287 RAHDTEFVEVIQLGKQL--SDGKPNMAETVAVIERLKAEQDAIHR--G-WAEKQKWLLQCVDLQM 1346
            ...:.::..::...:.|  ||| ..:...:|:.|.|...:...|:  | |.|:::.|:|....|:
  Rat  1184 DQREEDYARIVAASEALLASDG-AELGPGLALDEWLPHLEVGWHKLLGLWEERREALVQAHVYQL 1247

  Fly  1347 FNREADKIDATTKSHEAFLEYNNLGASLDEVEAILKRHLDFEKSLMAQDKILKGFSDNADKLISN 1411
            |.|:..:..|..::.|..|....|..:::.||..:|||.||..::....:.::.....|:.|:..
  Rat  1248 FLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMKRHRDFLTTMELNQQKMQVAVQAAEGLLRQ 1312

  Fly  1412 DHYDSKYIGDRRNQVLGKRKAVKDRAFERKRLLQASKDFHKFAAEADDLKVWLQDKTRIAGDENY 1476
            .:...:...:...::|.|.:..:.||.:..:.|........|..:..:|..|:.:|..:|.|...
  Rat  1313 GNAYGEQAQEAVARLLEKSQENQLRAQQWMQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTR 1377

  Fly  1477 RDLSNLPRKLQKHQAFERELRANEGQLRNVTKDGQALVQAGNRVPE----VESRVADLNKRWKDL 1537
            .|...|.::..:||||..||..|:..|..:.::||.|:|   ..||    |..::.::.:.|.:|
  Rat  1378 EDSHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQLMQ---EKPELAASVRKKLGEIRQCWAEL 1439

  Fly  1538 LTLSEDKGRKLEQAASQREHNRSLEDAKKKVDELDSALRSGDVGNDLRSCKDLINKQQILESEIT 1602
            .:.::.|.|:|.:|:...:..:|..:..|::..::|.|:..|.|.||.:....:.|.|.:||::.
  Rat  1440 ESTTQAKARQLFEASKADQLVQSFAELDKRLLHMESQLQDVDPGGDLATVNSQLKKLQSMESQVE 1504

  Fly  1603 IWDQKVAELVSTGDDMAHGGHFNAQN----IEAGTKEL--------QQRFKDLRDPTQRRRAKLE 1655
            .|.::|.||             .||.    :|..:|||        .:|...|.:|.|.||..|.
  Rat  1505 EWCREVGEL-------------QAQTAALPLEQASKELVGERQSAVGERLVRLLEPLQERRRLLL 1556

  Fly  1656 ESLNYHKFVFELDSEFQWINEHLPAAKSNELGQNLHQAQSLHKKHKKLEAEIKGHQPMINKALVA 1720
            .|...|:...:||.|..|:.|.||.|...|.|..|...|...||::.|..||:.|.|.:.:.|..
  Rat  1557 ASKELHQVAHDLDDELAWVQERLPLAMLTERGTGLQAVQQHIKKNQGLRREIQAHGPRLEEVLER 1621

  Fly  1721 GQSLISQQHPEREQVESLCQQLEQAWQDLERHCGERSRKLDMSLKAQQYLFDAGEIESWLGERNN 1785
            ...|.|.:.||.|.|....:||:.||..|......|.:.||.:.:.:||.||..|:|:||||:..
  Rat  1622 AGVLASLRSPEAEAVRRGQEQLQSAWTGLREATERRQQTLDAAFQVEQYYFDVAEVEAWLGEQEL 1686

  Fly  1786 VLRSTEYGRDRDSAAKLLTKHKTIELELDTYSGIVTEMGHSCAAMVAANHPDSKVLAAKQQLIEK 1850
            ::.|.:.|:|..|..:||.||..:|..::.|...:.::...|.|::...||||:.::.:|..:::
  Rat  1687 LMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQCRALLEMGHPDSEQISRRQSQVDR 1751

  Fly  1851 MLKSLHKLASQRQGRLMESLYKHEYFLESDEVEQWIREQEQAASSEDYGQDFEHLQLLQNKFDDL 1915
            :..:|.:|..:|:..|.:..:.::...:.||:|.||.|:|..|.|.:.||||||:.:||.||.:.
  Rat  1752 LYVALKELGEERRVSLEQQYWLYQLSRQVDELEHWIAEKEVVAGSPELGQDFEHVSVLQEKFSEF 1816

  Fly  1916 KHRV-EVGADRVDQCELLAKKLIDSESPYANEVEKRQEQLRTSWENLLQLLNQREQKLHAAGEIH 1979
            .... ..|.:|:.....:..:||:.....|..:.:.::.|..:|..||:|:..|.|.|.|:.|:|
  Rat  1817 ASETGTAGRERLAAVNQMVDELIECGHTAAATMAEWKDGLNEAWAELLELMGTRAQLLAASRELH 1881

  Fly  1980 RFHRDVAEALFRIQDKNAALSQELG--RDLNSALALLRKHEGFENDLVALEAQLQVLVEDSVRLQ 2042
            :|..|..|...:|::|...|.:...  ....||.::.|....||:||..|.:|::.|.|.:.:|:
  Rat  1882 KFFSDARELQGQIEEKRRRLPRLTAPPEPRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLR 1946

  Fly  2043 AKYP-SNASAIAQQQDKVVAAWNDLKERSTARGDRLAASSDLQTFLTDVRDIVSWSSNLRAALQA 2106
            ..|. .:|.|||.::.:|:..|.:|..........:::::|...|.:..||::||...:...:.|
  Rat  1947 TVYAGEHAEAIASREQEVLQGWKELLAACEDARLHVSSTADALRFHSQARDLLSWMDGIAGQIGA 2011

  Fly  2107 EEHVSDAAGATALKIQHDAIYGEIEAREDKFRYLNELSDSMVQTGHYAAADVEEKCAAMLDERQK 2171
            .:...|.:....|...|..:..|:|||..:.....||..|::......|.:::.:...:...:::
  Rat  2012 ADKPRDVSSVEVLMNYHQGLKTELEARVPELTACQELGRSLLLNKSAMADEIQAQLDKLGSRKEE 2076

  Fly  2172 LHAAWNKKKIMLEQKIDLFCFLRDAKQIDNLSSSQQAALSSSDFGQTVEDVQNKIRKHDEFERLI 2236
            :...|::....|:|.:::..|.::|...|...::|:..|.|.:.|.:|::|:..||:|:.|.:..
  Rat  2077 VSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAA 2141

  Fly  2237 QTQEEKVSLLQE-------------------HGRK--------------LIEQRHYDSANIQTIL 2268
            ...||:.|.|:.                   .|||              |:....||
  Rat  2142 AAWEERFSSLRRLTTIEKLKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYD-------- 2198

  Fly  2269 QGV--LARR------QKVKDLCAVRRYKLEDALLYAKFVRDCAEAKYWINEKQKKLEADAASYAE 2325
            :|:  ||||      .:|:......|.:|:...|..:..|        :.|...|:|..|.:.|.
  Rat  2199 RGLEPLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDR--------LPETSGKVEPAAPTAAA 2255

  Fly  2326 VTNLD--------EKIKKLQKHQ--AFQAEVAANQGRIQEIQDTGVILLSKQHESS----PEIKR 2376
            :...|        |:::...:.|  |.:||....:.|.:..:.|     .:..|::    ||.:.
  Rat  2256 LDTTDTPGTPAVTEQVRPRPERQELADRAEELPRRRRSERQEST-----DQPEEAARRRRPERQE 2315

  Fly  2377 AIE------IVLEAWQGLLAELEQRGRGLEEAQDSLE---FNSQLDKIEAWIRDKEMMVQASDTG 2432
            :.:      :.|..::.:....|:|.|.:|..:.|.:   ...:|.|.:|.:.|....:|..:.|
  Rat  2316 SADHEGPHSLTLGRYEQMERRRERRERRIERQESSEQETPTRGELVKGKATLADIVEQLQEKEAG 2380

  Fly  2433 RDLEHC------------NALMRKLDDVDSDMRVDDQRVK------------------------- 2460
            ..|...            ..|...|:..:...|.|..|.:
  Rat  2381 PGLPAAVPSLPQPRELPPGRLPNGLEPPERTPRPDRPRARDRPKPRRRPRPREGGEGGGSRRSRS 2445

  Fly  2461 ----------------HINQLADKLINQAQVPADTQSVDKRRKDFNYNWRQL-----QGALNAYR 2504
                            |..|....|:.:       :.:|..||..|.:|..|     :|.|..|:
  Rat  2446 APAQGGSAPAPPPPPTHTVQHEGFLLRK-------RELDANRKSSNRSWVNLYCVLSKGELGFYK 2503

  Fly  2505 ALLGGAN----------EIHVFNRDVDDTADRIAEKSLAMSSTDTGRDLAAVEALIRREEALERD 2559
            ...|.|:          .:|....:|  .:|...:|.:....|..|.:. .::|  :.||.:...
  Rat  2504 DSKGPASGGTHGGEPLLSLHKATSEV--ASDYKKKKHVFKLQTQDGSEF-LLQA--KDEEEMNGW 2563

  Fly  2560 MSAVKQKIDQHETAAEF 2576
            :.||...:.:|...|.:
  Rat  2564 LEAVANSVAEHAEIARW 2580

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
kst	NP_001097492.2	CH_beta_spectrin_rpt1	61..176	CDD:409042	64/115 (56%)
		CH_beta_spectrin_rpt2	196..302	CDD:409043	67/105 (64%)
		SPEC	450..640	CDD:238103	56/195 (29%)
		SPEC	658..832	CDD:238103	52/191 (27%)
		SH3	885..935	CDD:214620	2/49 (4%)
		SPEC	1030..1236	CDD:238103	40/209 (19%)
		SPEC	1134..1341	CDD:238103	43/213 (20%)
		SPEC	1343..1551	CDD:238103	49/211 (23%)
		SPEC	1449..1658	CDD:238103	58/224 (26%)
		SPEC	1555..1764	CDD:238103	65/220 (30%)
		SPEC	1765..1975	CDD:238103	63/210 (30%)
		SPEC	1873..2080	CDD:238103	61/210 (29%)
		SPEC	1979..2185	CDD:238103	47/208 (23%)
		SPEC	2188..2402	CDD:238103	54/274 (20%)
		SPEC	2407..2618	CDD:238103	39/241 (16%)
		SPEC	2514..2725	CDD:238103	13/63 (21%)
		SPEC	2726..2934	CDD:238103
		SPEC	2935..3145	CDD:238103
		SPEC	3042..3251	CDD:238103
		SPEC	3149..3357	CDD:238103
		SPEC	3359..3567	CDD:238103
		SPEC	3465..3680	CDD:238103
		PH_beta_spectrin	3801..3904	CDD:269975
Sptbn4	XP_008757363.1	CH_SF	73..212	CDD:469584	65/138 (47%)
		CH_SPTBN4_rpt2	214..343	CDD:409171	76/133 (57%)
		SPEC	361..584	CDD:238103	90/222 (41%)
		SPEC	479..693	CDD:238103	66/213 (31%)
		SPEC	819..1031	CDD:238103	62/370 (17%)
		SPEC	1135..1239	CDD:197544	24/104 (23%)
		SPEC	1245..1453	CDD:238103	49/210 (23%)
		SPEC	1351..1558	CDD:238103	58/222 (26%)
		SPEC	1560..1771	CDD:238103	66/210 (31%)
		SPEC	1666..1878	CDD:238103	64/211 (30%)
		Spectrin	1878..1977	CDD:395348	28/98 (29%)
		SPEC	1987..>2154	CDD:238103	38/166 (23%)
		PH_beta_spectrin	2466..2570	CDD:269975	23/115 (20%)