Protein Alignment kst and beta-Spec

DIOPT Version :10

• Sequence 1: NP_001097492.2
• Gene: kst / 38418
• FlyBaseID: FBgn0004167
• Length: 4337
• Species: Drosophila melanogaster
• Sequence 2: XP_061509680.1
• Gene: beta-Spec / 1271052
• VectorBaseID: AGAMI1_011991
• Length: 2363
• Species: Anopheles gambiae
• Alignment Length: 2607
• Identity: 793/2607 - (30%)
• Similarity: 1279/2607 - (49%)
• Gaps: 377/2607 - (14%)

  Fly     5 SVLRSNFSR---NEYRRYISYERQSLASQYEPGGYSALQTPTPSNRNSANMTQRDGIIKFENERI 66
            ||:|.:.|:   ||:...|.|:          ||.|:.:.                   ||..||
Mosquito     6 SVVRWDPSQGPGNEFIEDIEYD----------GGNSSSRL-------------------FERSRI 41

  Fly    67 KTLQEERLHIQKKTFTKWMNSFLIKAKMEVEDLFTDLADGIKLLKLLEIISSEKLGKPNSGRMRV 131
            |.|.|||..:||||||||:||.|::....::||:.|:.||..|:||||::|.|:|.:|..|:||:
Mosquito    42 KALAEERESVQKKTFTKWVNSHLVRVNSPIKDLYVDMRDGKNLIKLLEVLSGERLPRPTKGKMRI 106

  Fly   132 HKIENVNKSLAFL-HTKVRLESIGAEDIVDGNPRLILGLIWTIILRFQIQEIEIDVDEENESSEK 195
            |.:|||:|:|.|| ..:|.||:||:.||||||..|.||||||||||||||:|.|   ||.::.|.
Mosquito   107 HCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITI---EETDNKET 168

  Fly   196 RSAKDALLLWCQRKTHGYPGVNITDFTNSWRSGLGFNALIHSHRPDLFEYSTIVNSKNSNLDNLN 260
            :|||||||||||.||.||..||:.:||.|||.||.|||:||.|||||.::..:  ||.:.:.|||
Mosquito   169 KSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLIQFDKL--SKTNPIQNLN 231

  Fly   261 HAFDTAANELGIPSLLDAEDIDSARPDEKSILTYVASYYHTFARMKNEQKSGKRIANIVGQLMDA 325
            :||:.|..:||:..|||||||....||||||:|||.:|||.|:::|.|...||||..:||..||.
Mosquito   232 NAFNVAEEKLGLTKLLDAEDIFVDHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGIAMDN 296

  Fly   326 DRKKMQYEGLTTNLLSWIRQKTLELEQRDLPNSLEGIQRELLAFKEYRTIEKPPKYKERSEIEAL 390
            ||...:||.||:.||.||....::|..|...|||.|:|::|..|..|||:|||||:.|:..:|.|
Mosquito   297 DRMINEYESLTSELLKWIEVTIVQLGDRHFVNSLVGVQQQLAQFSNYRTVEKPPKFVEKGNLEVL 361

  Fly   391 YFTINTLLKALNQPPYNPQDGQLVNDIEKAWQILEYAEHHREVALRDELLRQEKLEQLNYKFEKK 455
            .||:.:.::|.||.||.|::|::::||.|||:.||.|||.||:|||:||:||||||||..:|.:|
Mosquito   362 LFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAARFNRK 426

  Fly   456 SVLREGYLKEMIQVLSDPRY---LRQVDATLKKHEAISADILARVERFNDLTAMAEELDRENYHG 517
            :.:||.:|.|..:::|...:   |..|:|..||||||..||.|..||...:.|:..||:.|.||.
Mosquito   427 ATMRETWLSENQRLVSTDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCNELEAEKYHD 491

  Fly   518 KERVRRREQEVMAKWRQLLELLENQRLNLSQMSNLMNLLREIASTTEAVRELQQQFASEDVGPHL 582
            .||:..|:..|:..|..|:|||..:|:.|.....|....:|:....:::.|::|:..::|.|.||
Mosquito   492 IERIAARKDNVLRLWNYLIELLRARRMRLEFSIQLQQNFQEMIYILDSMEEIKQRLLTDDYGKHL 556

  Fly   583 LGVEELLQAHSLQELQVNTYGETLKRFNRQALPYKSSEHK-----DAALLAQRLADLEEAYSELL 642
            :|||:|||.|||.|..:|..|:.:|:..:.:..:...|..     |.:::..|:..||:||:||.
Mosquito   557 MGVEDLLQKHSLVEADINVLGDRVKQVVQNSQKFLVDEEDNYKPCDPSIIVDRVQRLEDAYAELC 621

  Fly   643 RRSAARRARLEEARNFHHFMEDYDNEESWLVDKQRICKTGITAKDLRAVLSLQQKHKALEDEIKS 707
            :.:..||:||||.|....|..|..:||:|:.:|::|........||..|..|..||||||.||:|
Mosquito   622 KLAVERRSRLEENRKLWQFYWDMADEENWIKEKEQIVSADEIGHDLTTVNLLLSKHKALESEIQS 686

  Fly   708 RKPKSGQMSTAGKRLIGEQHPRSSEIQSRIDSLAEHWQALEALVELRRRQLEDAAEAYQFYTDAN 772
            .:|:...:|..|..|:...|..:..|..|:..:...|..|..|.|.||::||||.:.:|.:.||:
Mosquito   687 HEPQLMAVSEVGDELVRRGHFGADRIDERLKEILAMWSNLRELTEYRRKRLEDAVDYFQLFADAD 751

  Fly   773 EAESWLNEKIALVNSRDYGNDEPSAQALLQRHRDLQGELNAYSGDILNLNQQADKLIKAGICTLE 837
            :.::|:.:.:.||:|.|.|.||.:.|:||::|:|:..||..|:..|..|:.||.:|
Mosquito   752 DIDNWMLDALRLVSSEDVGRDEANVQSLLKKHKDVADELKNYAETIEQLHAQAKRL--------- 807

  Fly   838 LSAAEPELPEVEQEEWVNETRLVPKEVWEDEWVEKLEHKKVTETKMLPHVKSLFPFEGQGMKMDK 902
                  .|.|.|||.
Mosquito   808 ------TLNEPEQER-------------------------------------------------- 816

  Fly   903 GEVMLLKSKTNDDWWCVRKDNGVEGFVPANYVREVEPRPVACIVPKAEKVKSLQKVKKTILVRQV 967
                                                                             
Mosquito   817 ----------------------------------------------------------------- 816

  Fly   968 VPVKRIKPVSVAPKPLVQRRTSTQSINENADSVEKRQQRINQTYDELQEMAQKRHALLEDSIHLF 1032
                                     :||...:::.|       |.||.|:|:.|...|.|::.|:
Mosquito   817 -------------------------VNERLAAIDNR-------YRELMELAKLRKQRLLDALSLY 849

  Fly  1033 GFYRECDDFEKWMKEKERMIKS----DEGEGVDNAKRKFEKFITDLSAASKRVEEIDGAVDTFRR 1093
            ....|.|..|:|:.|||||:::    .:.|.|:..|.:::.|..:::|.:.||..::........
Mosquito   850 KLISESDGVEQWIGEKERMLQTMVPGKDIEDVEIMKHRYDGFDKEMNANASRVAVVNQLARQLLH 914

  Fly  1094 QGHSQLDKIIARQRQIHQIWQRLNNAKAQREKSLEGASSVELFNRTCDEAKVWMSE-KMLQLDTA 1157
            ..|....:||.||..::..|.:|......:...|:.|..|:.|...|.|...|:.: |.:..:|.
Mosquito   915 VEHPNSPEIIERQNHLNNSWSKLREQAESKRDDLKSAHGVQTFYIECRETVSWIEDKKRILTETD 979

  Fly  1158 VITPDLRTVQALQRRHQNLERELAPVEDKVNRVTYLGNSVKNAYPAEKDNVNARQQEVQDMWQQV 1222
            .:..||..|..||||...:||:||.::.|:..:....::::..:|.|...:..|...:|.:|:|:
Mosquito   980 SLQMDLTGVMTLQRRLSGMERDLAAIQAKLTALENEADAIEGDHPEEAALIRERVATIQTIWEQL 1044

  Fly  1223 QQRGSDLRNRIESEVGQQVFNNSAKVLLAWIDSVKDQLNADESARDVETANNLLKKHNDLGDDIR 1287
            .|...:..:::|.......|........||:...:..:.::::...:..|..||.:|..:.::|.
Mosquito  1045 TQMLKERDSKLEEAGDLHRFLRDLDHFQAWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEID 1109

  Fly  1288 AHDTEFVEVIQLGKQLSDGKPNMAET---VAVIERLKAEQDA---IHRGWAEKQKWLLQCVDLQM 1346
            .:..::.:::..|:.|: .:|...|.   :.:.|||||.:|.   :|:.|..:|..|.|.:|.|:
Mosquito  1110 NYTEDYKKMMDYGEGLT-SEPTQTEDPQYMFLRERLKALKDGWEELHQMWENRQVLLSQGLDQQL 1173

  Fly  1347 FNREADKIDATTKSHEAFLEYNNLGASLDEVEAILKRHLDFEKSLMAQDKILKGFSDNADKLISN 1411
            |||:|.:.:......|..|..::...:|::.|..||||..|..::.|.|:........|.::.|.
Mosquito  1174 FNRDARQAEVLLSQQEHVLSKDDTPVNLEQAENQLKRHEAFLTTMEANDEKFNTIVQVAGQMTSK 1238

  Fly  1412 DHYDSKYIGDRRNQVLGKRKAVKDRAFERKRLLQASKDFHKFAAEADDLKVWLQDKTRIAGDENY 1476
            ||:|:..|..|...:..:|...::||.|....|:.....|:|..:.::|..|:|:|...|.|:.|
Mosquito  1239 DHFDADKITKRAESIAHRRDDNRNRALELHEKLKNQVKLHEFLQDIEELTEWVQEKYITAQDDTY 1303

  Fly  1477 RDLSNLPRKLQKHQAFERELRANEGQLRNVTKDGQALVQAGNRVPE-VESRVADLNKRWKDLLTL 1540
            |....:..|..:|||||.|:.||:.:|....|..|.|:.......| :|.::.||:|.:.:|.|.
Mosquito  1304 RSAKTVHSKWTRHQAFEAEIAANKERLHEAKKAAQELMVEKPEFKEIIEPKLTDLSKNFDELETS 1368

  Fly  1541 SEDKGRKLEQAASQREHNRSLEDAKKKVDELDSALRSGDVGNDLRSCKDLINKQQILESEITIWD 1605
            :::||..|..|..:....:|::|....:|:|:..:.:.|.||||.|...|:.||||:::::.:..
Mosquito  1369 TKEKGALLFDAKREVIVQQSVDDIDSWMDDLEKQIINTDTGNDLTSVNILMQKQQIIQTQMAVKA 1433

  Fly  1606 QKVAELVSTGDDMAHGGHFNAQN--IEAGTKELQQRFKDLRDPTQRRRAKLEESLNYHKFVFELD 1668
            ::|.||....:.:......:...  :|..| .:..||:.::.|...|:.:||:.....:|..:::
Mosquito  1434 RQVEELDKQTEVLTKTAPSDVLEPIVEKKT-AVNARFEKIKAPLLERQRQLEKKKEAFQFRRDVE 1497

  Fly  1669 SEFQWINEHLPAAKSNELGQNLHQAQSLHKKHKKLEAEIKGHQPMINKALVAGQSLISQQHPERE 1733
            .|..||:|.:|.|:|.|.|.:|.....|.|||:.|..||..|:|.|......||.||.:.|.:..
Mosquito  1498 DEKLWIDEKMPLAESTEYGNSLFNVHVLKKKHQSLNTEIDNHEPRIMTICNNGQKLIDEGHEDAG 1562

  Fly  1734 QVESLCQQLEQAWQDLERHCGERSRKLDMSLKAQQYLFDAGEIESWLGERNNVLRSTEYGRDRDS 1798
            ....|..||.|.||:|:.....|.|:||.|.|.|||.|||.|.|||:.|:...:...:.|:|..|
Mosquito  1563 SYADLISQLTQKWQELKDAVENRHRQLDQSEKVQQYFFDAAEAESWMSEQELYMMVEDRGKDETS 1627

  Fly  1799 AAKLLTKHKTIELELDTYSGIVTEMGHSCAAMVAANHPDSKVLAAKQQLIEKMLKSLHKLASQRQ 1863
            |..|:.||:::|..::.|:..:.::|.:...:....|..|..::.||..::|:...|..||.:|:
Mosquito  1628 AQNLMKKHESLEQSVEDYADTIRQLGETARQLTTEQHAYSDQVSVKQSQLDKLYAGLKDLAGERR 1692

  Fly  1864 GRLMESLYKHEYFLESDEVEQWIREQEQAASSEDYGQDFEHLQLLQNKFDDL-KHRVEVGADRVD 1927
            .||.|:|.......|.|::||||.::|..|.|.:.|||::|:.||..:|::. :....||::||.
Mosquito  1693 ARLDEALQLFMLSREVDDLEQWITDREVVAGSHELGQDYDHITLLWERFNEFAQDTATVGSERVA 1757

  Fly  1928 QCELLAKKLIDSESPYANEVEKRQEQLRTSWENLLQLLNQREQKLHAAGEIHRFHRDVAEALFRI 1992
            :...:|..||.:....:..:.:.::.|..||::||:|:..|:..|.|:.|:|:|..|..:.|.||
Mosquito  1758 KANGIADDLIHAGHSDSATIAEWKDGLNESWQDLLELIETRKAMLAASRELHKFFHDCKDVLGRI 1822

  Fly  1993 QDKNAALSQELGRDLNSALALLRKHEGFENDLVALEAQLQVLVEDSVRLQAKYP-SNASAIAQQQ 2056
            .:|...:|:|||||.....||.|||:.|..||:.|.:|:|.:.|:|.:|||.|. ..|..|..::
Mosquito  1823 NEKQHGVSEELGRDAGVVSALQRKHQNFIQDLMTLHSQVQQIQEESAKLQAAYAGEKAREITNRE 1887

  Fly  2057 DKVVAAWNDLKERSTARGDRLAASSDLQTFLTDVRDIVSWSSNLRAALQAEEHVSDAAGATALKI 2121
            .:|:.||..|:.....|..:||.:.||..|...||.::.|..::...:...|...|.:|...|..
Mosquito  1888 HEVLNAWAHLQSMCEERRGKLADTGDLFKFFNMVRTLMLWMEDVVRQMNTSEKPRDVSGVELLMN 1952

  Fly  2122 QHDAIYGEIEAREDKFRYLNELSDSMVQTGHYAAADVEEKCAAMLDERQKLHAAWNKKKIMLEQK 2186
            .|.::..||:.|||.|.....|...::...|||:||::::...:.:.|..|...|.::...|:..
Mosquito  1953 NHQSLKAEIDTREDNFSACLALGKELLSRNHYASADIKDRLLQLTNSRNALLHRWEERWENLQLI 2017

  Fly  2187 IDLFCFLRDAKQIDNLSSSQQAALSSSDFGQTVEDVQNKIRKHDEFERLIQTQEEKVSLLQEHGR 2251
            ::::.|.|||...:....:|:..|.|::.|.|:::|:|.|:||:.||:....|||:.|.|:
Mosquito  2018 LEVYQFARDAAVAEAWLIAQEPYLMSTELGHTIDEVENLIKKHEAFEKSAAAQEERFSALE---- 2078

  Fly  2252 KLIEQRHYDSANIQTILQGVLARRQKVKDLCAVRRYKLEDALLYAKFVRDCAEAKYWINEKQKKL 2316
                       .:.|.....:.|||                        |.||     ..::::|
Mosquito  2079 -----------RLTTFELKEMKRRQ------------------------DAAE-----EAERQRL 2103

  Fly  2317 EADAASYAEVTNLDEKIKKLQKHQAFQAEVAA------NQGRIQEIQDTGVILLSKQHESSPEIK 2375
            ||:||  |.....:.:.:.:::.:|..|..||      :..|.|| ...|..|:|::|...|:.:
Mosquito  2104 EAEAA--AARAAAEAEAEAIRQQEAAAARDAADAPGSPHSTREQE-SVAGETLVSRRHSGVPKER 2165

  Fly  2376 RAIEIVLEA------------------WQ--------GLLAELEQR---GRGLEEAQDSLEFNSQ 2411
            .:......|                  |:        |..::.|.|   |...|.||:.:
Mosquito  2166 SSSSASASAGVKVSRRSRSKSPFRSFRWKRTASKADDGAASDDEDRPSPGGADEGAQEGI----- 2225

  Fly  2412 LDKIEAWIRDKEMMVQASDTGRDLEHCNALMRKL---DDVDSDMRVDDQ---------------- 2457
            |.:...|   :....:||:...|..:|.|...:|   .|..|...|.:|
Mosquito  2226 LTRKHEW---ESTTKKASNRSWDKVYCVARSGRLTFFKDQRSAKSVPEQTFRGEPPLELKGAQIE 2287

  Fly  2458 ------RVKHINQLADKLIN---------------------QAQ-------------VPADTQSV 2482
                  :.||:.::  ||.|                     :||             :||.:|..
Mosquito  2288 IASDYTKKKHVFRI--KLSNGGEFLLQCHDDAEMQQWVTALKAQCELDASGEGRSLTLPASSQKD 2350

  Fly  2483 DKRRKDF 2489
            :::|:.|
Mosquito  2351 EQKRRSF 2357

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
kst	NP_001097492.2	CH_beta_spectrin_rpt1	61..176	CDD:409042	65/115 (57%)
		CH_beta_spectrin_rpt2	196..302	CDD:409043	63/105 (60%)
		SPEC	450..640	CDD:238103	66/197 (34%)
		SPEC	658..832	CDD:238103	61/173 (35%)
		SH3	885..935	CDD:214620	0/49 (0%)
		SPEC	1030..1236	CDD:238103	53/210 (25%)
		SPEC	1134..1341	CDD:238103	48/213 (23%)
		SPEC	1343..1551	CDD:238103	62/208 (30%)
		SPEC	1449..1658	CDD:238103	60/211 (28%)
		SPEC	1555..1764	CDD:238103	63/210 (30%)
		SPEC	1765..1975	CDD:238103	66/210 (31%)
		SPEC	1873..2080	CDD:238103	72/208 (35%)
		SPEC	1979..2185	CDD:238103	66/206 (32%)
		SPEC	2188..2402	CDD:238103	53/248 (21%)
		SPEC	2407..2618	CDD:238103	24/142 (17%)
		SPEC	2514..2725	CDD:238103
		SPEC	2726..2934	CDD:238103
		SPEC	2935..3145	CDD:238103
		SPEC	3042..3251	CDD:238103
		SPEC	3149..3357	CDD:238103
		SPEC	3359..3567	CDD:238103
		SPEC	3465..3680	CDD:238103
		PH_beta_spectrin	3801..3904	CDD:269975
beta-Spec	XP_061509680.1	None