Protein Alignment nSMase and ISC1

DIOPT Version :9

• Sequence 1: NP_647790.1
• Gene: nSMase / 38396
• FlyBaseID: FBgn0035421
• Length: 442
• Species: Drosophila melanogaster
• Sequence 2: NP_010935.1
• Gene: ISC1 / 856739
• SGDID: S000000821
• Length: 477
• Species: Saccharomyces cerevisiae
• Alignment Length: 505
• Identity: 114/505 - (22%)
• Similarity: 200/505 - (39%)
• Gaps: 142/505 - (28%)

  Fly     6 LNILTLNIWGIPYVSSDRRPRIDAICKELA---------------------SGKYDIVSLQEVWA 49
            :..||.|.||:.|||..|:.|:.||..:||                     :..||:::|||:|.
Yeast    39 IKFLTFNTWGLKYVSKHRKERLRAIADKLAGHSMLTPISDELLPNGGDSNENEDYDVIALQEIWC 103

  Fly    50 QEDSELLQKGTEAVLPHSHYFHSGVM-GAGLLVLSKYPILGTLFHAWSVNGYFHRIQHADWFGGK 113
            .||.:.|.....:..|:...||||:: |.||.:|||.||..|..:.:.:||....:...||:.||
Yeast   104 VEDWKYLASACASKYPYQRLFHSGILTGPGLAILSKVPIESTFLYRFPINGRPSAVFRGDWYVGK 168

  Fly   114 GVGLCRILVGGQMVHLYNAHLHAEYDNAND-EYKTHRVIQAFDTAQFIEATRGNSALQILAGDLN 177
            .:.:..:..|.:.:.:.|:|:||.|....| .|..||..||:|.::.|:..|......|:.||||
Yeast   169 SIAITVLNTGTRPIAIMNSHMHAPYAKQGDAAYLCHRSCQAWDFSRLIKLYRQAGYAVIVVGDLN 233

  Fly   178 AQPQDISYKVLLYTSKMLDS----------------------------CDS--DSFRTNECEHNS 212
            ::|..:.:|.|...:.::||                            |||  :::|.
Yeast   234 SRPGSLPHKFLTQEAGLVDSWEQLHGKQDLAVIARLSPLQQLLKGCTTCDSLLNTWRA------- 291

  Fly   213 YTSKQARERNP-LGIRIDHIFVRGGDHVNAEIAEYKLPFPERVPGEKFSFSDHEAVMAKLKLFKL 276
                   :|.| ...|:|:..:   |....:..:..:.|.||:|....|.|||.|.
Yeast   292 -------QRQPDEACRLDYALI---DPDFLQTVDAGVRFTERIPHLDCSVSDHFAY--------- 337

  Fly   277 EPRSEEPVATIEVNCLVEDGETCSVREVGAGDALTGEDDQSSQHQPEIQCNGSSTSIQSMPAART 341
                                 :|::..|          .|.::.:|       |||::.......
Yeast   338 ---------------------SCTLNIV----------PQGTESRP-------STSVKRAKTHDR 364

  Fly   342 AALLEALALCDASLLQLNT----------DRILYYSAATFLFVLLVLLVEFTA-PVGMRTIFLLL 395
            ..:|:..:..:..:..::|          .|.|::.|:..|.:..:::..||| ..|..:||.:|
Yeast   365 ELILQRYSNYETMIECIHTYLKTAQRQKFFRGLHFWASILLLIASLVVTTFTANKAGWSSIFWVL 429

  Fly   396 KFI-------VFGVILFCVFMASIWNYMERNGVLQGKKSMEVMLHHAQKY 438
            ..|       :.|.|.|      ::...|...:::.::.:....||.|.:
Yeast   430 FAIAVSISGTIDGAISF------LFGRSEIRALIEVEQEVLDAEHHLQTF 473

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nSMase	NP_647790.1	EEP	12..267	CDD:294334	83/308 (27%)
ISC1	NP_010935.1	EEP	41..341	CDD:412407	87/346 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157345351
Domainoid	1	1.000	122	1.000	Domainoid score	I1234
eggNOG	1	0.900	-	-		E1_KOG3873
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	124	1.000	Inparanoid score	I1289
Isobase	1	0.950	-	0	Normalized mean entropy	S2849
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003990
OrthoInspector	1	1.000	-	-		oto99668
orthoMCL	1	0.900	-	-		OOG6_105043
Panther	1	1.100	-	-	LDO	PTHR12393
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2164
SonicParanoid	1	1.000	-	-		X2751
TreeFam	1	0.960	-	-
	14	13.730