DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Shab and KCNQ1

DIOPT Version :9

Sequence 1:	NP_001189037.1	Gene:	Shab / 38352	FlyBaseID:	FBgn0262593	Length:	1607	Species:	Drosophila melanogaster
Sequence 2:	NP_000209.2	Gene:	KCNQ1 / 3784	HGNCID:	6294	Length:	676	Species:	Homo sapiens

Alignment Length:	616	Identity:	145/616 - (23%)
Similarity:	229/616 - (37%)	Gaps:	161/616 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   448 QKYLWELLEKPN------TSFAARVIAVISILFIVLSTIALTLNTLPQLQHIDNGTPQDNPQLAM 506
            |..::..||:|.      ..||..:|.::.::|.|||||       .|...:..||      |..
Human   107 QGRVYNFLERPTGWKCFVYHFAVFLIVLVCLIFSVLSTI-------EQYAALATGT------LFW 158

  Fly   507 VEAVCITWFTLEYILRF------SASPDKW---KFFKGGLNIIDLLAILPYFVSLFLLETNKNAT 562
            :|.|.:.:|..||::|.      |.....|   :|.:..::||||:.::...|.|.:  .:|...
Human   159 MEIVLVVFFGTEYVVRLWSAGCRSKYVGLWGRLRFARKPISIIDLIVVVASMVVLCV--GSKGQV 221

  Fly   563 DQFQDVRRVVQVFRIMRILRILKLARHSTGLQSLGFTLRNSYKELGLLMLFLAMGVLIFSS-LAY 626
            .....:|.:    |.::|||:|.:.|.....:.||..:....:|| :..|::....||||| ..|
Human   222 FATSAIRGI----RFLQILRMLHVDRQGGTWRLLGSVVFIHRQEL-ITTLYIGFLGLIFSSYFVY 281

  Fly   627 FAEKDEKD----TKFVSIPETFWWAGITMTTVGYGDIYPTTALGKVIGTVCCICGVLVIALPIPI 687
            .||||..:    .:|.|..:..||..:|:||:||||..|.|.:||.|.:...:..:...|||..|
Human   282 LAEKDAVNESGRVEFGSYADALWWGVVTVTTIGYGDKVPQTWVGKTIASCFSVFAISFFALPAGI 346

  Fly   688 IVNNFAEFYKNQMRREKALKRR-----EALDRAKR----EGSIVSFHHINLKDAFAKSMDLI--- 740
            :.:.|| ....|.:|:|...|:     ..:..|.|    |....|...|.::.| .:|..|:
Human   347 LGSGFA-LKVQQKQRQKHFNRQIPAAASLIQTAWRCYAAENPDSSTWKIYIRKA-PRSHTLLSPS 409

  Fly   741 -----DVIVDTGK----QTNVVHPKGKRQSTPNI-----GRQTLDVQSAPGHNLSQTDG------ 785
                 .|:|...|    :.|.|.|..|..:.|:|     ..:.||..|..|::.|....
Human   410 PKPKKSVVVKKKKFKLDKDNGVTPGEKMLTVPHITCDPPEERRLDHFSVDGYDSSVRKSPTLLEV 474

  Fly   786 --------NS------TEGESTSGRNPATTGTGCYKNYDHVANLRNSNLHNRR------------ 824
                    ||      .|||:.  ..|.|          |::.||.   |:|.
Human   475 SMPHFMRTNSFAEDLDLEGETL--LTPIT----------HISQLRE---HHRATIKVIRRMQYFV 524

  Fly   825 GSSSEQDAVPPYSFDNPNARQTSMMAMESYRREQQALLQQQQQQQQQMLQMQQIQQKAPNGNG-- 887
            .....|.|..||..                   :..:.|..|.....|::::::|::.....|
Human   525 AKKKFQQARKPYDV-------------------RDVIEQYSQGHLNLMVRIKELQRRLDQSIGKP 570

  Fly   888 -------------GATGGGVANNLAMVAASSAATAVATATN-----ASNASNTAPGSEG--AEGG 932
                         |:...|...|......:.....:|..|:     .|....:.|||.|  .|||
Human   571 SLFISVSEKSKDRGSNTIGARLNRVEDKVTQLDQRLALITDMLHQLLSLHGGSTPGSGGPPREGG 635

  Fly   933 GD-----GDGGGVDDDNLSQAKGLPIQMMIT 958
            ..     |.||.||.:....:..||....:|
Human   636 AHITQPCGSGGSVDPELFLPSNTLPTYEQLT 666

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Shab	NP_001189037.1	BTB	309..416	CDD:197585
		BTB_2	309..408	CDD:280393
		Ion_trans	501..700	CDD:278921	61/212 (29%)
		Ion_trans_2	613..696	CDD:285168	32/87 (37%)
KCNQ1	NP_000209.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..28
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	62..84
		Ion_trans	153..359	CDD:278921	64/219 (29%)
		Ion_trans_2	270..349	CDD:285168	30/78 (38%)
		Selectivity filter. /evidence=ECO:0000250	312..317		3/4 (75%)
		Interaction with CALM. /evidence=ECO:0000269\|PubMed:25441029	370..382		2/11 (18%)
		KCNQ_channel	<511..617	CDD:281513	15/124 (12%)
		Interaction with CALM, calcium-dependent. /evidence=ECO:0000269\|PubMed:25441029	515..529		0/13 (0%)
		Interaction with KCNE1 C-terminus. /evidence=ECO:0000269\|PubMed:25037568	535..572		7/55 (13%)
		Interaction with AKAP9. /evidence=ECO:0000269\|PubMed:11799244	588..616		4/27 (15%)
		C-terminal assembly domain. /evidence=ECO:0000269\|PubMed:10654932	589..620		5/30 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	620..676		15/47 (32%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810