Protein Alignment CG32301 and Adcy8

DIOPT Version :10

• Sequence 1: NP_728724.2
• Gene: CG32301 / 38285
• FlyBaseID: FBgn0052301
• Length: 1111
• Species: Drosophila melanogaster
• Sequence 2: NP_058838.1
• Gene: Adcy8 / 29241
• RGDID: 2036
• Length: 1248
• Species: Rattus norvegicus
• Alignment Length: 1141
• Identity: 302/1141 - (26%)
• Similarity: 499/1141 - (43%)
• Gaps: 200/1141 - (17%)

  Fly    20 GYEWK-----LLRYKCRELRLEGVYHRHC---HLVSVALVTQLIIVIEILMLIHVILLFSV---- 72
            ||.::     .||...:...||.:|.|:.   ...|..::..|.::.::.:|:..:.|.|.
  Rat   143 GYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDP 207

  Fly    73 VKNIDFLTVLPYFAVMLLTPSILMPSREPNVE----QYESIAILVSCLMALLLTAMDLILPICYF 133
            :|.|    :|.:|..:.:....|:..|:....    ||..:...|:....:|...:...|
  Rat   208 LKGI----LLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGL----- 263

  Fly   134 RPFSLVPSYDHVVIVLI--YLMFPIAFVKNGRAYFLGLAVSLFYFGYMALIDKISTLDKVWELTA 196
                |.....:|:..|.  |.|.|:...   .|...||..||.......||.:::.. .:.::.|
  Rat   264 ----LGDGIGYVLFTLFATYSMLPLPLT---WAILAGLGTSLLQVTLQVLIPRLAVF-SINQVLA 320

  Fly   197 YGAYLFFLNMLCMFLSRFQEYNMRSGILSRYQVVYQNLVFQMAMKEEKALLDSIIP----VTLAR 257
            .......:|...:|:|...:...|...|...:.|...|..:...:.::.|:.|::|    :.:..
  Rat   321 QVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMIN 385

  Fly   258 SLQDAIASHIEEDPSNLMPFTKTRHLFMEPHPEVSILEADMVDFTGLTTTMEVSDLVAILHELFV 322
            .:.:....|::.         :...:::..:..||||.||:..||.|:||:...:||.:|:|||.
  Rat   386 DMTNVEDEHLQH---------QFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFA 441

  Fly   323 SFDLAANHNRATRIKFLGDSYTCVTGIPSYFPTHANACVNQALDMIEISREVSKRRNKKIDLRIG 387
            .||..|:.:...|||.|||.|.||:|:|.....||:.||...|.||:..|.|..|....:|:|||
  Rat   442 RFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIG 506

  Fly   388 VHSGEILAGIIGLTKWQFDIWSKDVDITNRLESSGLPGMVHISSRTLGLLDNHYVYEEGTDTAKL 452
            :|||.:|.|::||.|||||:||.||||.|:|||.|:||.:|||..||..|...|..|||....:
  Rat   507 IHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKER- 570

  Fly   453 DPLLQRSNLSTYLIRS------RLPNFEDTDDLEDDNFSLNDYRFS------FSFSQDYEDIQVK 505
            :..|::.|:.||||:.      .||     :|:..::.|.:|.|.|      .|:|.:.....:.
  Rat   571 NEFLRKHNIETYLIKQPEESLLSLP-----EDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIV 630

  Fly   506 AQRDMILEVEHMPVNRVQTCKIRRPMH-RIAKEDINEEY-------------RFHLESYYLFTTF 556
            .:::.:..:....:|.:.. .:.:.:| :...|:||:..             |.|::.:.|.  |
  Rat   631 GKQNTLAALTRNSINLLPN-HLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLM--F 692

  Fly   557 RSWRMEWSFNKMRDLLMKYSL--GMMVFAGATIIAMDLLVKSEAMDYTMLFSLFLLILLPLLLAS 619
            :...:|..:::|||.:.|.:|  ..:|....|.| ..||..|..|..|:.||:.:::...|:|.:
  Rat   693 KDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAI-QSLLPSSRLMPMTIQFSILIMLHSALVLIT 756

  Fly   620 ----YKKLWLRGRRLSPITQPTFFLSRWFIKASDLIEKSVFVRIPLAIMVLFLLYVMSSETVFSC 680
                ||.|.|..|:.......|:......|.||              |::.||..|::   :..|
  Rat   757 TAEDYKCLPLILRKTCCWINETYLARNVIIFAS--------------ILINFLGAVIN---ILWC 804

  Fly   681 DIARLELEIINSELHNLRPQM------LCFMPWGVTYAVVIVLSLMLVIIGIPLIIKLGVGLAIL 739
            |..:      :..|.||....      :|..|....:..|:.:....|.:.:..::||.|.|.::
  Rat   805 DFDK------SIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMI 863

  Fly   740 GSHVITVHAYYGFAFERSQTTN------IGVTSSLAHSWYMVAFFIVVVVREG-YLNYILKASYF 797
            ..:.:.....|...|......|      :|...:   |..::|.|::.|...| .|.|..:..:.
  Rat   864 AIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEA---SLLLMAMFLLAVFYHGQQLEYTARLDFL 925

  Fly   798 MSMCFEKKHELTKVK--TRTIKIIMANILPTHVAEVFKVR-RRSDQLYYENFSQVAVMFATIENY 859
            ..:  :.|.|:.::|  ....:.::.||||.|||..|..: |.:::||.:::..|.||||:|..:
  Rat   926 WRV--QAKEEINEMKDLREHNENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGF 988

  Fly   860 -------EADKSG---LRALHEMICYFDELLVNYQSWYKIEKIKVMGWTYLAACGLHVDHYTDFS 914
                   |.:..|   ||.|:|:|..||||| ....:..|||||.:|.||:|..|
  Rat   989 ADFYSQTEMNNQGVECLRLLNEIIADFDELL-GEDRFQDIEKIKTIGSTYMAVSG---------- 1042

  Fly   915 VSVPISTNRESDKLQKSGSVRFAPMDGDEIMIKDLHPTQATTNEDDNTILVMTEFALNLLRILRD 979
                                              |.|.:....:....:..:.:|:|.|...:::
  Rat  1043 ----------------------------------LSPEKQQCEDKWGHLCALADFSLALTESIQE 1073

  Fly   980 IRSKGIFFEKDSKLTGSLKIGIAHGPVMAGVVGLSKPHYDIWGHTVNMASRMTSTGVRDGIHVTE 1044
            |       .|.|.....|:|||:||.|:|||:|..||.|||||.|||:||||.||||...|.|.|
  Rat  1074 I-------NKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPE 1131

  Fly  1045 STANVLRDFNIRCTYRGMTFVKGV----GQVPTYLV 1076
            .|..:|:|......|||..:|||:    |::.||.:
  Rat  1132 ETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFL 1167

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG32301	NP_728724.2	AcyC	51..445	CDD:441717	117/407 (29%)
		Nucleotidyl_cyc_III	283..439	CDD:448371	76/155 (49%)
		AcyC	681..1076	CDD:441717	117/424 (28%)
		Nucleotidyl_cyc_III	841..1076	CDD:448371	82/248 (33%)
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269|PubMed:16258073, ECO:0000269|PubMed:22494970, ECO:0000269|PubMed:22976297	1..179		8/35 (23%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269|PubMed:22976297	1..106
		Essential for CALM1 interaction. /evidence=ECO:0000269|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	51..117
		AcyC	211..564	CDD:441717	112/378 (30%)
		Guanylate_cyc	402..586	CDD:425528	86/184 (47%)
		Adcy_cons_dom	614..709	CDD:461877	15/97 (15%)
		Guanylate_cyc	970..1169	CDD:425528	82/250 (33%)
		Involved in CALM1 interaction. /evidence=ECO:0000269|PubMed:16258073	1106..1248		30/62 (48%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269|PubMed:19305019	1197..1212
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1220..1248