DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment alpha-Spec and Actn1

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	XP_038968920.1	Gene:	Actn1 / 81634	RGDID:	70907	Length:	914	Species:	Rattus norvegicus

Alignment Length:	920	Identity:	238/920 - (25%)
Similarity:	394/920 - (42%)	Gaps:	261/920 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly  1630 KSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKH--QLVEADIVAHED 1692
            |:...|..|.|..:|:       |              ||......|:.:|  :|::...:..:|
  Rat   157 KTAPYKNVNIQNFHIS-------W--------------KDGLGFCALIHRHRPELIDYGKLRKDD 200

  Fly  1693 RIKDMNN---------------QADSLVESGQFDT--------------AGIQEKRQSINERYER 1728
            .:.::|.               .|:.:|.:.:.|.              :|.|:...:.|    |
  Rat   201 PLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAAN----R 261

  Fly  1729 ICNLAAHRQARLNEALTLHQFFRDIADEESWIK------------------EKKLLVGSDDYGRD 1775
            ||.:.|..|.  ||.| :..:.:..:|...||:                  ::||    :|: ||
  Rat   262 ICKVLAVNQE--NEQL-MEDYEKLASDLLEWIRRTIPWLENRVPENTMQAMQQKL----EDF-RD 318

  Fly  1776 LTGVQNLKKKHKRLEAELG----------SHEPAIQAVQEAGEKLMDVSNL-GVPEIEQRLKALN 1829
            ...:....|..::.:.|:.          |:.||.  :...|..:.|::|. |.  :||..|...
  Rat   319 YRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAF--MPSEGRMVSDINNAWGC--LEQAEKGYE 379

  Fly  1830 QAWA--ELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGD-SMAAVQGLLKK 1891
            : |.  |::.|     ::||.  ..::|..:....|||...|:.:|..:||.. :::.::.||||
  Rat   380 E-WLLNEIRRL-----ERLDH--LAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKK 436

  Fly  1892 HDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDNS 1956
            |:|||:|..||:||...|.....||.|...:...|:..|||::..:.|||.||..:|:.||
  Rat   437 HEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREAL---- 497

  Fly  1957 AYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALKD 2021

  Rat   498 ----------------------------------------------------------------- 497

  Fly  2022 QLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNSW 2086
                                            .|.::||      ..|::|||.:||:|:.||:|
  Rat   498 --------------------------------ERTEKLL------ETIDQLYLEYAKRAAPFNNW 524

  Fly  2087 FENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQK----IKSFNV---GP 2144
            .|.|.|||.|....::||||:.|..||.||:|:|..|:.:..|:..:..:    :::::|   |.
  Rat   525 MEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGT 589

  Fly  2145 NPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRYYML 2209
            ||||..|.:.:...|.::::::..||..|.:|..||:.|::|||:|...||:...|: :|:...:
  Rat   590 NPYTTITPQEINGKWDHVRQLVPRRDQALTEEHSRQQHNERLRKQFGAQANVIGPWI-QTKMEEI 653

  Fly  2210 GYNRQGTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILDNRYT----EHS 2270
                 |...:|..|:||.||..||.....:...:..:.::|....|::|.||.||::|    ||.
  Rat   654 -----GRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHI 713

  Fly  2271 TVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEFKSC 2335
            .||    |:||.....|..:.:|.||..|:..|:|::.:.||...|.|||:|.||.|..:|||:|
  Rat   714 RVG----WEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKAC 774

  Fly  2336 LRALGYDL---PMVEEGQPD-----------------PEFEAILDVVDPNRDGYVSLQEYIAFMI 2380
            |.:||||:   |..:.|..|                 .||..|:.:|||||.|.|:.|.:|.||
  Rat   775 LISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFM- 838

  Fly  2381 SKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDAL 2445
            |:||.:..:.:::..:|: |.|.|:.|:|.:||...|..|.|:||:.||.|::.|.|   :..||
  Rat   839 SRETADTDTADQVMASFK-ILAGDKNYITGDELRRELPPDQAEYCIARMAPYAGPDS---VPGAL 899

  Fly  2446 DYIDFTRTLF 2455
            ||:.|:..|:
  Rat   900 DYMSFSTALY 909

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742
		SPEC	1074..1287	CDD:238103
		SPEC	1182..1425	CDD:238103
		SPEC	1426..1638	CDD:238103	2/7 (29%)
		SPEC	1533..1744	CDD:238103	26/144 (18%)
		SPEC	1646..1850	CDD:238103	47/265 (18%)
		SPEC	1852..2062	CDD:238103	40/210 (19%)
		SPEC	1958..2176	CDD:238103	43/224 (19%)
		SPEC	2072..2296	CDD:238103	77/234 (33%)
		EFh	2311..2379	CDD:238008	34/87 (39%)
		EFhand_Ca_insen	2386..2456	CDD:400872	24/70 (34%)
		SPEC	48..258	CDD:238103
		SPEC	154..364	CDD:238103
		SPEC	261..470	CDD:238103
		SPEC	471..680	CDD:238103
		SPEC	682..893	CDD:238103
		Spectrin	893..>961	CDD:395348
Actn1	XP_038968920.1	CH_ACTN_rpt1	28..132	CDD:409063
		CH_ACTN_rpt2	136..250	CDD:409065	16/113 (14%)
		SPEC	277..498	CDD:238103	62/338 (18%)
		SPEC	395..622	CDD:238103	80/333 (24%)
		Spectrin	634..733	CDD:395348	30/108 (28%)
		EFh	750..839	CDD:238008	36/89 (40%)
		EFhand_Ca_insen	844..910	CDD:400872	24/70 (34%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.920