DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment alpha-Spec and Sptan1

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	XP_008759895.1	Gene:	Sptan1 / 64159	RGDID:	621714	Length:	2498	Species:	Rattus norvegicus

Alignment Length:	2530	Identity:	1597/2530 - (63%)
Similarity:	1950/2530 - (77%)	Gaps:	112/2530 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 PKEVKILETVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEKLQAA 70
            |..||:|||.|||||||:|||.||:.||..:..:|:|||||.|||:|:|||:|||.||.||||.|
  Rat     3 PSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVA 67

  Fly    71 SEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQQHFASESIQVRLDELHKLWE 135
            |:|:|:||||||.|:||||||||||.|:|.|||.||.||..||::.|||||:|:.||.|||:.||
  Rat    68 SDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWE 132

  Fly   136 LLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKD 200
            |||.::.|||:||.||..|||:||:||:||.||.|||..||::|.|||||||||||:||:|||.|
  Rat   133 LLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTD 197

  Fly   201 MASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNR 265
            :|:.|.||.||||.|.||:|:.|||.:.|..::||:|.||||||.||:.||.|||||.|:|||||
  Rat   198 LAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNR 262

  Fly   266 DADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHAD 330
            |.|||:.||.||:.:::|||:||||||||||.|||||:|||||||||||..|.|||.||...|..
  Rat   263 DVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPL 327

  Fly   331 HSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAKD 395
            .::||:.|:.|:...|:.:.|.|.||..:||:||.|.|||||||||.||:..|||:|:|||||.|
  Rat   328 SANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELAND 392

  Fly   396 VAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAEIQEKLAALENDKSSLLSLWE 460
            ||||||||:|||||||||||.|||||...||||.||...|||:.|::|||:.|..::::||.|||
  Rat   393 VAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWE 457

  Fly   461 DRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAAQEEK 525
            .||..||||||||||||||||.|.||:||||||.||||||||||||||:||||||||||:|||||
  Rat   458 LRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEK 522

  Fly   526 IKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDSNRYQQFERDCDETKG 590
            |.|||.||||||...|||.:|||.||..||:||.||.|::..||..|.||...|||.||.||.|.
  Rat   523 ITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSDELKS 587

  Fly   591 WISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTELIEKQHYAADQINTR 655
            |::||:|.|||::|.||:||.||:||||.||.||:||:|||:.:...|.:||:..|||.:::..|
  Rat   588 WVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEEVAAR 652

  Fly   656 MQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLLSEDHGKDLTSVQNLQ 720
            |.|::.||:.|::|::.||.||.||.|||||||.:|||||||.|:||.|.|:|:|||||:|||||
  Rat   653 MNEVISLWKKLLEATELKGVKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQ 717

  Fly   721 KKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEGNLSARYAALAAPMGERKQHLLD 785
            ||||||||||.||||||:.|.:.|.:|.::|||||:||:.|:..|.|||.||..||..|||.|.|
  Rat   718 KKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLAD 782

  Fly   786 SLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKKHQAVLAEINNHEARLLNVISSG 850
            ||::||||||:|||..|||||||||||||||:||||||||:|||||:.|||..||.|:..|...|
  Rat   783 SLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKG 847

  Fly   851 ENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSLQAHQYFADANEAESWMREKEPI 915
            ..|:::..||::|::.:|..|.::|..||.|:|||:|||:|||||.|||||||||||||||||||
  Rat   848 NAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPI 912

  Fly   916 ATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVVALYD 980
            ...:||||||||:||||||||||:|||.|:|::||||:|||::||||..|:.|.||||.|:||||
  Rat   913 VGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYD 977

  Fly   981 YTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDAGLSASQQNLVDNH-SI 1044
            |.|||||||:|||||:||||||.||||||||||||||||||||:||:|...|||::||::.. ||
  Rat   978 YQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSI 1042

  Fly  1045 AKRQNQINSQ--------------------YDNLLALARERQNKLNETVKAYVLVREAADLAQWI 1089
            |.||.||::|                    |.:||.|..:|:..|.::.|.::|.|||.:|.|||
  Rat  1043 ALRQGQIDNQTRITKEAGSVSLRMKQVEELYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWI 1107

  Fly  1090 RDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLG---------- 1144
            .:|| .|..::.||.|||:|||||||||||..||||||.||.::|::|..|.|.|
  Rat  1108 NEKE-AALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQ 1171

  Fly  1145 QTE--------------------AALKIQT-----QMQDLNEKWNNLQTLTAEKASQLGSAHEVQ 1184
            |.|                    |.|.:.|     .:::|||:|.:||.|..|::..||||||||
  Rat  1172 QQEVYGMMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQ 1236

  Fly  1185 RFHRDIDETKDWIAEKANALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQ 1249
            |||||.||||:||.||..|||.|:.|.||.|||.|||||||.|||||||.||:..|.|||.||:|
  Rat  1237 RFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQRLIQ 1301

  Fly  1250 SHPDTAEQTYAKQKEINEMWDQIITKSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDE 1314
            |||::||....|..|:|:.|..:..::..||.||.||:||||||||:|||::|||.:..||:|||
  Rat  1302 SHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDE 1366

  Fly  1315 LANDVTGAEALIERHQARRAEIGFTLGISSAPGAAASTSSIASPSGDEEHRTEIDARAGTFGAFE 1379
            ||.||||||||:||||                                |||||||||||||.|||
  Rat  1367 LAKDVTGAEALLERHQ--------------------------------EHRTEIDARAGTFQAFE 1399

  Fly  1380 QFGNELLQANHYASPEIKEKIEDLAKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAR 1444
            |||.:||...||||||||||::.|.:.|.||||||.:||:.|:..|:|||:.||||.||:||:||
  Rat  1400 QFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAR 1464

  Fly  1445 EAFLNADDDANAGGNVEALIKKHEDFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEKRKQV 1509
            |||||.:|..::..:||||||||||||||||..|:||||||..||||||.:|||...:..:|.:|
  Rat  1465 EAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEV 1529

  Fly  1510 LERWRHLKEGLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQ-----SKH 1569
            |:|||.||..:|||||:||:.|||||||||.||||.||:||||.|::||||||.|||     |||
  Rat  1530 LDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKH 1594

  Fly  1570 QKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEYLTHKTTEKSLKL 1634
            ||||||||||.||||||:.|:.||.:||::..|:||||||:.||..:||||::|..|:.|||.||
  Rat  1595 QKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKL 1659

  Fly  1635 KEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIKDMNN 1699
            ||||||:.:...:||.||||.|||:||.:||.|||||||.||:|||||:||||.|||||:||:|:
  Rat  1660 KEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNS 1724

  Fly  1700 QADSLVESGQFDTAGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKK 1764
            |||||:.|..|||:.::|||.:||.|:::|.::|..|:|:|:|:..|||||||:.||||||||||
  Rat  1725 QADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWIKEKK 1789

  Fly  1765 LLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALN 1829
            |||.|:||||||||||||:||||||||||.:||||||.|.:.|:||.|.:.:|..||:|||....
  Rat  1790 LLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRLAQFV 1854

  Fly  1830 QAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLKKHDA 1894
            :.|.|||.|||.|||:|:|||.||||:|.||||||||.||..|::.|||||::||:|||||||:|
  Rat  1855 EHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEA 1919

  Fly  1895 FETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDNSAYL 1959
            ||||||.||||.:.:|..|.:|::..|||.|:|:.:.:.|..|:.:|...||:||..|.:|||:|
  Rat  1920 FETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFL 1984

  Fly  1960 QFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALKDQLI 2024
            ||.||||||||||.:|||.:::|::|||||:|||||||||||||||.||:||||.|||||||||:
  Rat  1985 QFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLL 2049

  Fly  2025 NASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNSWFEN 2089
            .|.|.||.||..||..::.||.:|...|.|||.:||..|..||::|:|:||||||||||||||||
  Rat  2050 AAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFEN 2114

  Fly  2090 AEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWFTMEA 2154
            |||||||||||||:|||:|||:||..|::|||||:|||..||.||::||||.|..||||||||||
  Rat  2115 AEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEA 2179

  Fly  2155 LEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRYYML---GYNR--- 2213
            ||||||||||||:||:.||.||.:|||||||||:|||:|||.||||:.|||.|:|   .|.|
  Rat  2180 LEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIR 2244

  Fly  2214 ----------QGTS-MMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILDNRYT 2267
                      .|.| |:|.||:||.||||.:.|..|:||.|..|||||:|||.:||.|||||:||
  Rat  2245 VYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYT 2309

  Fly  2268 EHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLNHQEF 2332
            |||||||||||||||||.|||||||||||||||.:||:|::|||||||||||||||||:||||||
  Rat  2310 EHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEF 2374

  Fly  2333 KSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQSYEEIENAF 2397
            |||||:|||||||||||:||||||||||.|||||||:||||||:|||||:|||||:|.||||:||
  Rat  2375 KSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAF 2439

  Fly  2398 RAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTLFQN 2457
            ||:::..:|||||||||.|||::.|||||..|||:.:.: |:.:..|.||::|||:||.|
  Rat  2440 RALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGK-GRELPTAFDYVEFTRSLFVN 2498

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742	44/51 (86%)
		SPEC	1074..1287	CDD:238103	122/247 (49%)
		SPEC	1182..1425	CDD:238103	139/242 (57%)
		SPEC	1426..1638	CDD:238103	141/216 (65%)
		SPEC	1533..1744	CDD:238103	137/215 (64%)
		SPEC	1646..1850	CDD:238103	132/203 (65%)
		SPEC	1852..2062	CDD:238103	129/209 (62%)
		SPEC	1958..2176	CDD:238103	155/217 (71%)
		SPEC	2072..2296	CDD:238103	170/240 (71%)
		EFh	2311..2379	CDD:238008	61/67 (91%)
		EFhand_Ca_insen	2386..2456	CDD:400872	39/69 (57%)
		SPEC	48..258	CDD:238103	138/209 (66%)
		SPEC	154..364	CDD:238103	129/209 (62%)
		SPEC	261..470	CDD:238103	132/208 (63%)
		SPEC	471..680	CDD:238103	131/208 (63%)
		SPEC	682..893	CDD:238103	134/210 (64%)
		Spectrin	893..>961	CDD:395348	53/67 (79%)
Sptan1	XP_008759895.1	Spectrin	44..147	CDD:278843	69/102 (68%)
		SPEC	150..361	CDD:238103	129/210 (61%)
		SPEC	362..572	CDD:238103	149/209 (71%)
		SPEC	468..678	CDD:238103	132/209 (63%)
		SPEC	679..890	CDD:238103	134/210 (64%)
		Spectrin	890..>958	CDD:278843	53/67 (79%)
		SH3_Alpha_Spectrin	971..1023	CDD:212742	44/51 (86%)
		SPEC	1092..1339	CDD:238103	122/247 (49%)
		SPEC	1234..1445	CDD:238103	139/242 (57%)
		SPEC	1446..1663	CDD:238103	141/216 (65%)
		Spectrin	1663..1767	CDD:278843	62/103 (60%)
		SPEC	1770..1979	CDD:238103	131/208 (63%)
		SPEC	1876..2087	CDD:238103	129/210 (61%)
		SPEC	1983..2201	CDD:238103	155/217 (71%)
		SPEC	2101..2338	CDD:238103	167/236 (71%)
		EFh	2353..2421	CDD:238008	61/67 (91%)
		EF-hand_7	2356..2421	CDD:290234	58/64 (91%)
		EFhand_Ca_insen	2428..2496	CDD:285885	38/68 (56%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353877
Domainoid	1	1.000	171	1.000	Domainoid score	I3644
eggNOG	1	0.900	-	-		E1_KOG0040
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2353
Inparanoid	1	1.050	3002	1.000	Inparanoid score	I13
OMA	1	1.010	-	-		QHG51850
OrthoDB	1	1.010	-	-		D15759at33208
OrthoFinder	1	1.000	-	-		FOG0004911
OrthoInspector	1	1.000	-	-		oto98665
orthoMCL	1	0.900	-	-		OOG6_100348
Panther	1	1.100	-	-	LDO	PTHR11915
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X5245
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.810