DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment alpha-Spec and actn2b

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	NP_001032662.1	Gene:	actn2b / 641575	ZFINID:	ZDB-GENE-051127-39	Length:	895	Species:	Danio rerio

Alignment Length:	781	Identity:	212/781 - (27%)
Similarity:	363/781 - (46%)	Gaps:	186/781 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly  1709 QFDTA--------GIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIKEKKL 1765
            |.:||        |:.::.:.:.|.|||   ||:.....:...:   .:..:.|.|::.::.::.
Zfish   262 QAETAANRICKVLGVNQENEKLMEDYER---LASELLEWIRRTI---PWLENRAAEKTMVEMQRK 320

  Fly  1766 LVGSDDYGRDLTGVQNLKKKHKRLEAELG----------SHEPAIQAVQEAGEKLMDVSNL--GV 1818
            |....||.|    :....|..::.:.|:.          |:.||.  :...|:.:.|:::.  |:
Zfish   321 LEDFRDYRR----MHKPPKVQEKCQLEISFNTLQTKLRISNRPAF--MPSEGKMVSDIASAWQGL 379

  Fly  1819 PEIEQRLKALNQAW--AELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDY-GD 1880
            .:.|:..    :.|  .|::.|  .|.:.|.|     :|..:....|:|.|.|:::|:.:|| ..
Zfish   380 EQAEKGY----EEWLLTEIRRL--ERVEHLAE-----KFRQKASNHESWATGKEEMLTRKDYESA 433

  Fly  1881 SMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALA 1945
            |:..|:.||:||:|||:|.:||:||...|.....||.|...|...|:.||||.:....|:|..|.
Zfish   434 SLMEVRALLRKHEAFESDLSAHQDRVEQIAAIAQELNELDYHDVASVNQRCQSICDLWDHLGTLT 498

  Fly  1946 ARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQ 2010
            .||:.||
Zfish   499 QRRREAL---------------------------------------------------------- 505

  Fly  2011 EGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLT 2075
                                                       .|.::||      ..:::|:|.
Zfish   506 -------------------------------------------ERTEKLL------ETVDQLFLE 521

  Fly  2076 FAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKI--- 2137
            :||:::.||:|.|.|.|||.|....::|||::.|..||.||:|:||.|:|:.:|:..:.|::
Zfish   522 YAKRSAPFNNWMEGAMEDLQDMFIVHTIEEVQTLIAAHEQFKATLSEADAERQAILGIQQEVLKI 586

  Fly  2138 -KSFNVG---PNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFH 2198
             :|:.:.   .|||:..|.|.:...|..::|::.:||..|.:|..||..|::||::||..|||..
Zfish   587 FQSYGIRGDLTNPYSTITAEEIAIKWDKVKKLVPQRDSALQEELARQHANERLRRQFAAQANLIG 651

  Fly  2199 QWLTETRYYMLGYNRQGTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILD 2263
            .|: :||...:|:    .||..| |:||.|:..|:.....:.:.:.::.::|....|::|.||.|
Zfish   652 PWI-QTRMEEIGH----CSMAMG-GTLEDQMTQLKQYEHVIVSYKSNIDRLEGDHQLIQESLIFD 710

  Fly  2264 NRYT----EHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKS 2324
            |::|    ||..||    |:.|.....|..:.:|.||..|:..|:|::.:.||...|.|||:.::
Zfish   711 NKHTNYTMEHVRVG----WELLLTTIARTINEIETQILTRDAKGISQEQMHEFRSSFNHFDRKEN 771

  Fly  2325 GKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQS 2389
            |.::..:|::||.::||||..|       ||..|:.:||||..|.||.|.:|.|| ::||.:..:
Zfish   772 GGMDSDDFRACLISMGYDLGEV-------EFARIMMLVDPNASGNVSFQSFIDFM-TRETADTDT 828

  Fly  2390 YEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTL 2454
            .|::..:|: |.|||:||:..:||...|..|.|:||:.||.|:..|.:   :..||||..|:..|
Zfish   829 AEQVIASFK-ILAADKPYILSDELRRELPPDQAEYCISRMPPYCGPGA---LPGALDYTAFSTAL 889

  Fly  2455 F 2455
            :
Zfish   890 Y 890

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742
		SPEC	1074..1287	CDD:238103
		SPEC	1182..1425	CDD:238103
		SPEC	1426..1638	CDD:238103
		SPEC	1533..1744	CDD:238103	10/42 (24%)
		SPEC	1646..1850	CDD:238103	31/162 (19%)
		SPEC	1852..2062	CDD:238103	42/210 (20%)
		SPEC	1958..2176	CDD:238103	42/224 (19%)
		SPEC	2072..2296	CDD:238103	78/234 (33%)
		EFh	2311..2379	CDD:238008	27/67 (40%)
		EFhand_Ca_insen	2386..2456	CDD:400872	25/70 (36%)
		SPEC	48..258	CDD:238103
		SPEC	154..364	CDD:238103
		SPEC	261..470	CDD:238103
		SPEC	471..680	CDD:238103
		SPEC	682..893	CDD:238103
		Spectrin	893..>961	CDD:395348
actn2b	NP_001032662.1	CH	43..141	CDD:214479
		CH	153..258	CDD:237981
		Spectrin	282..391	CDD:278843	22/124 (18%)
		SPEC	403..630	CDD:238103	83/338 (25%)
		Spectrin	638..740	CDD:278843	34/111 (31%)
		EFh	758..820	CDD:238008	29/69 (42%)
		EFhand_Ca_insen	825..891	CDD:285885	25/70 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.920