DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment alpha-Spec and actn3a

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	NP_571597.1	Gene:	actn3a / 58037	ZFINID:	ZDB-GENE-000329-9	Length:	896	Species:	Danio rerio

Alignment Length:	865	Identity:	218/865 - (25%)
Similarity:	381/865 - (44%)	Gaps:	229/865 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly  1666 SGKDLASVQNLMKKHQ--LVEADIVAHEDRIKDMNN---------------QADSLVESGQFDT- 1712
            |.||..::..|:.:|:  |::...:..:|.|.::|.               .||.:|.:.:.|.
Zfish   181 SWKDGLALCALIHRHRPDLIDYSKLRKDDPIGNLNTAFDIAEKFLDIPKMLDADDIVNTPKPDEK 245

  Fly  1713 -------------AGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWIK--- 1761
                         ||.::...:.|    |||.:.|..|.  ||.| :.::.:..::...||:
Zfish   246 AIMTYVSCFYHAFAGAEQAETAAN----RICKVLAVNQE--NEKL-MEEYEKLASELLEWIQRTI 303

  Fly  1762 ---------------EKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELG----------SHEPAIQ 1801
                           ::||    :|: ||...|....|..::.:.|:.          |:.||.
Zfish   304 PWLENRVAEQTMHAMQQKL----EDF-RDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAF- 362

  Fly  1802 AVQEAGEKLMDVSNL--GVPEIEQRLKALNQAW--AELKNLAATRGQKLDESLTYQQFLAQVEEE 1862
             :...|:.:.|:::.  |:.::|:..    :.|  .|::.|     ::||.  ..::|..:....
Zfish   363 -MPSEGKMVSDIADAWKGLEQVEKGY----EEWLLTEIRRL-----ERLDH--LAEKFRQKSTLH 415

  Fly  1863 EAWITEKQQLLSVEDYGD-SMAAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGES 1926
            ::|.|.|::|||.:||.. |:..::.|::||:|||:|..||:||...|.....||.|...:...:
Zfish   416 QSWTTGKEELLSQKDYETASLMEIRALMRKHEAFESDLAAHQDRVEQIAAIAQELNELDFYDAAT 480

  Fly  1927 IAQRCQQLRLKLDNLSALAARRKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTV 1991
            |..:||.:..:.|||..|..:|:.:|
Zfish   481 INAQCQGICDQWDNLGTLTQKRRESL--------------------------------------- 506

  Fly  1992 QTLLTKQETFDAGLNAFEQEGIHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRK 2056
                                                               .|.:||
Zfish   507 ---------------------------------------------------ERVEKL-------- 512

  Fly  2057 DRLLAMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLS 2121
                     :..|::|||.|||:|:.||:|.:.|.|||.|....:|||||::|..||.||:|:|.
Zfish   513 ---------WETIDQLYLEFAKRAAPFNNWMDGAMEDLQDMFIVHSIEEIQSLITAHDQFKATLP 568

  Fly  2122 SAEADFKALAALDQKIKSFN-------VGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKR 2179
            .|:.:..|:..:..::....       |..||||..:.:.:...|..:::::..||..|.:|..|
Zfish   569 EADKERMAIMGIHSEVLKIAQTYGIKIVSENPYTVLSHQNIVNKWEAVKQLVPMRDQMLQEEVAR 633

  Fly  2180 QEENDKLRKEFAKHANLFHQWLTETRYYMLGYNRQGTSMMEGSGSLEQQLEALRVKATEVRARRV 2244
            |:.|::||::||..||:...|: :|:...:.:     ..::.:||||:|:..|:.....:...:.
Zfish   634 QQSNERLRRQFAAQANIIGPWI-QTKMEEISH-----VSIDIAGSLEEQMSNLKTYEQNIINYKS 692

  Fly  2245 DLKKIEELGALLEEHLILDNRYT----EHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVS 2305
            ::.|:|....|.:|.||.||::|    ||..||    |:||.....|..:.:|.||..|:..|:|
Zfish   693 NIDKLEGDHQLSQEGLIFDNKHTNYTMEHVRVG----WEQLLTTIARTINEVENQILTRDAKGIS 753

  Fly  2306 EDSLKEFSMMFKHFDKDKSGKLNHQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYV 2370
            ::.|.||...|.|||:.::|.::..:|::||.::||||..|       ||..|:.:||||..|.|
Zfish   754 QEQLNEFRASFNHFDRKRNGMMDPDDFRACLISMGYDLGEV-------EFARIMTLVDPNNTGVV 811

  Fly  2371 SLQEYIAFMISKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEP 2435
            :.|.:|.|| ::||....:.|::..:|: |.|:|:.|:|.|||...|..:.|:||:.||..:
Zfish   812 TFQAFIDFM-TRETAETDTAEQVMASFK-ILASDKAYITVEELRRELPPEQAEYCISRMTKY--- 871

  Fly  2436 RSGQPIKDALDYIDFTRTLF 2455
            ..|.....|||||.|:..|:
Zfish   872 MGGDAPPGALDYISFSSALY 891

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742
		SPEC	1074..1287	CDD:238103
		SPEC	1182..1425	CDD:238103
		SPEC	1426..1638	CDD:238103
		SPEC	1533..1744	CDD:238103	23/108 (21%)
		SPEC	1646..1850	CDD:238103	46/246 (19%)
		SPEC	1852..2062	CDD:238103	37/210 (18%)
		SPEC	1958..2176	CDD:238103	42/224 (19%)
		SPEC	2072..2296	CDD:238103	74/234 (32%)
		EFh	2311..2379	CDD:238008	26/67 (39%)
		EFhand_Ca_insen	2386..2456	CDD:400872	24/70 (34%)
		SPEC	48..258	CDD:238103
		SPEC	154..364	CDD:238103
		SPEC	261..470	CDD:238103
		SPEC	471..680	CDD:238103
		SPEC	682..893	CDD:238103
		Spectrin	893..>961	CDD:395348
actn3a	NP_571597.1	CH	41..139	CDD:278723
		CH	154..259	CDD:237981	13/77 (17%)
		Spectrin	283..392	CDD:278843	19/120 (16%)
		SPEC	404..631	CDD:238103	76/333 (23%)
		Spectrin	639..742	CDD:278843	31/112 (28%)
		FRQ1	752..896	CDD:227455	56/152 (37%)
		EFh	759..821	CDD:238008	28/69 (41%)
		EFhand_Ca_insen	826..892	CDD:285885	24/70 (34%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.920