DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment alpha-Spec and SPTBN4

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	NP_066022.2	Gene:	SPTBN4 / 57731	HGNCID:	14896	Length:	2564	Species:	Homo sapiens

Alignment Length:	2036	Identity:	490/2036 - (24%)
Similarity:	829/2036 - (40%)	Gaps:	363/2036 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 IQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHE--KLQAASEESYRDPTN 80
            :.||......|:...:....::..:|:::|....|..|.|.|..|:.:  :|.||.:..: |..:
Human   744 LAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFGH-DEAS 807

  Fly    81 LQAKIQKHQAFEAEVSAHSNAIVSL----------DNTGQEMINQQHFASESIQVRLDELHKLWE 135
            .:...::|:|...||.||...:..|          ...|..::        ::|||:.|..:|:.
Human   808 SRRLARQHRALTGEVEAHRGPVSGLRRQLATLGGASGAGPLVV--------ALQVRVVEAEQLFA 864

  Fly   136 LLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKD 200
            .:....|.:...|:.||.:.:...:......||.:||.::.:......|:.|||:|.:|:...::
Human   865 EVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLSMRVPDSLDDVEVVQHRFESLDQE 929

  Fly   201 MASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNR 265
            |.|...||.:||....:||:.|||..|.:...::.||..|.|:.:|...|:|::.....::....
Human   930 MNSLMGRVLDVNHTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVL 994

  Fly   266 DADETVAWIAEK-DVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHA 329
            :..|..|.:.|| ..|.|:...|      .|||.:..|:|..|.|||.:.:.|..||..|.....
Human   995 EVAEVRAQVREKRRAVESAPRAG------GALQWRLSGLEAALQALEPRQAALLEEAALLAERFP 1053

  Fly   330 DHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAK 394
            ..:.::.....|:...|.:|.:.|:...:.:..:..|.|||.|....:.|      ::.|.|.|.
Human  1054 AQAARLHQGAEELGAEWGALASAAQACGEAVAAAGRLQRFLHDLDAFLDW------LVRAQEAAG 1112

  Fly   395 DVAG--------AEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAE------IQEKL 445
            ...|        |:|||.||...|.|:|.||:.:.....:.:.||..:   .||      :.|.|
Human  1113 GSEGPLPNSLEEADALLARHAALKEEVDQREEDYARIVAASEALLAAD---GAELGPGLALDEWL 1174

  Fly   446 AALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIK 510
            ..||.....||.|||.||....|....|||.||..||...:..||..|:..:|..:::|||..:|
Human  1175 PHLELGWHKLLGLWEARREALVQAHIYQLFLRDLRQALVVLRNQEMALSGAELPGTVESVEEALK 1239

  Fly   511 KHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDS 575
            :|.||..::...::|::.....|..|:...:...:...:....||.:....|.::.:..|.|.|.
Human  1240 QHRDFLTTMELSQQKMQVAVQAAEGLLRQGNIYGEQAQEAVTRLLEKNQENQLRAQQWMQKLHDQ 1304

  Fly   576 NRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTE 640
            ...|.|.|||.|..|||.||:..|.|.:..|...|:.:..:||.|..||..||..:|.|...|.:
Human  1305 LELQHFLRDCHELDGWIHEKMLMARDGTREDNHKLHKRWLRHQAFMAELAQNKEWLEKIEREGQQ 1369

  Fly   641 LIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLL 705
            |::::...|..:..::.||...|..|...:..|..:|.||.:..|..::..:::..|..:|.||.
Human  1370 LMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKKLLHMESQLQ 1434

  Fly   706 SEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADN------------I 758
            ..|.|.||.:|.:..||...:|:.|             ...:.|.|...|..            :
Human  1435 DVDPGGDLATVNSQLKKLQSMESQV-------------EEWYREVGELQAQTAALPLEPASKELV 1486

  Fly   759 RNKEGNLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQ 823
            ..::..:..|...|..|:.||::.||.|.::.|:..||:||.||::|:.|:|..|.||..|..||
Human  1487 GERQNAVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMQTERGNGLQAVQ 1551

  Fly   824 NLIKKHQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQD 888
            ..|||:|.:..||..|..||..|:.....:...:...::.:|:.||.||..|..|:|.:.:|:|.
Human  1552 QHIKKNQGLRREIQAHGPRLEEVLERAGALASLRSPEAEAVRRGLEQLQSAWAGLREAAERRQQV 1616

  Fly   889 LDDSLQAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQ 953
            ||.:.|..||:.|..|.|:|:.|:|.:....|.||||.|:..|||||..|...:|.:..:|..|.
Human  1617 LDAAFQVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLS 1681

  Fly   954 EQAKNCRQQETPVVDITGKECVVALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGF 1018
            .|   ||                                     .||...:.|
Human  1682 RQ---CR-------------------------------------ALLEMGHPD------------ 1694

  Fly  1019 VPAAYIKKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAA 1083
                           |:|       |::||:|::..|..|..|..||:..|.:....|.|.|:.:
Human  1695 ---------------SEQ-------ISRRQSQVDRLYVALKELGEERRVALEQQYWLYQLSRQVS 1737

  Fly  1084 DLAQWIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDL-KANEVRLANMNEIAVQLTSLGQTE 1147
            :|..||.:||..|...: :|:|.|.|.|||:||.:|..:. .|...|||.:|::..:|...|.|.
Human  1738 ELEHWIAEKEVVAGSPE-LGQDFEHVSVLQEKFSEFASETGMAGRERLAAVNQMVDELIECGHTA 1801

  Fly  1148 AALKIQTQMQDLNEKWNNLQTLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLGKD 1212
            ||...:.: ..|||.|..|..|...:|..|.::.|:.:|..|..|.:..|.||...|.......:
Human  1802 AATMAEWK-DGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTTPPE 1865

  Fly  1213 LR-SVQTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHP-DTAEQTYAKQKEINEMWDQIITK 1275
            .| |..::||.....|.||..|..::|||.|.|.:|...:. :.||...::::|:.:.|.::::.
Human  1866 PRPSASSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLSA 1930

  Fly  1276 STARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQARRAEIGFTL 1340
            ....:..:..:.|..||.|..||||:|::.:.|.:.:.:...||:..|.|:..||
Human  1931 CEDARLHVSSTADALRFHSQVRDLLSWMDGIASQIGAADKPRDVSSVEVLMNYHQ---------- 1985

  Fly  1341 GISSAPGAAASTSSIASPSGDEEHRTEIDARAGTFGAFEQFGNELLQANHYASPEIKEKIEDLAK 1405
            |:                      :||::||.......::.|..||......:.||:.:::.|..
Human  1986 GL----------------------KTELEARVPELTTCQELGRSLLLNKSAMADEIQAQLDKLGT 2028

  Fly  1406 AREDLEKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHEDF 1470
            .:|::.:.|......|:|.|::..:.::..:|::|::|:|..|.:.:..::...||.||::||.|
Human  2029 RKEEVSEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAF 2093

  Fly  1471 DKAINGHEQKIAALQTV-------ADQ-------LIAQNHYASNLVDEKRKQVLERWRHLKEGL- 1520
            .||....|::.::|:.:       |:|       |:.:..:........:...|.|....:.||
Human  2094 RKAAAAWEERFSSLRRLTTIEKIKAEQSKQPPTPLLGRKFFGDPTELAAKAAPLLRPGGYERGLE 2158

  Fly  1521 -----------IEKRSRLG------DEQTLQQFSRDADEIENWI-AEKLQLATEESYKDPANIQS 1567
                       .|.|:|:|      ..:.||.......||...: ...|..|.|::.:.||...:
Human  2159 PLARRASDTLSAEVRTRVGYVRQELKPERLQPRIDRLPEIPGRVEPAALPAAPEDAAETPATPAA 2223

  Fly  1568 KHQKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEY-LTHKTTEKS 1631
            ..|...  ..|...:|||.:. |........::....||:|.::|..:..:..|: ..|..|...
Human  2224 AEQVRP--RPERQESADRAEE-LPRRRRPERQESVDQSEEAARRRRPERQESAEHEAAHSLTLGR 2285

  Fly  1632 LKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIKD 1696
            .:..|..::|.               |..|..::|.:....::..:.|.:...||||   :::::
Human  2286 YEQMERRRERR---------------ERRLERQESSEQEMPIRGDLVKGKATLADIV---EQLQE 2332

  Fly  1697 MNNQADSLVESGQFDTAG--IQEKRQ----------SINERYERICNLAA------HRQARLNEA 1743
            .        |:|....||  :.:.|:          .:.||..|.....|      .|:.|..|
Human  2333 K--------EAGPGLPAGPSLPQPRELPPGRLPNGLELPERTPRPDRPRARDRPKPRRRPRPRE- 2388

  Fly  1744 LTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGS----HEPAIQAVQ 1804
                                    |.:..|         .::.:...|:.||    ..|....||
Human  2389 ------------------------GGEGGG---------SRRSRSAPAQGGSAPAPPPPPTHTVQ 2420

  Fly  1805 EAGEKLMDVSNLGVPEIEQRLKALNQAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEK 1869
            ..|..|..      .|::...|:.|::|..| ....::|:
Human  2421 HEGFLLRK------RELDANRKSSNRSWVSL-YCVLSKGE------------------------- 2453

  Fly  1870 QQLLSVEDYGDSMAAVQG-------LLKKHDA---FETDFTAHKDRCSLICDQGSE-LVEAKNHH 1923
                 :..|.||.....|       ||..|.|   ..:|:...|....|....||| |::||:..
Human  2454 -----LGFYKDSKGPASGSTHGGEPLLSLHKATSEVASDYKKKKHVFKLQTQDGSEFLLQAKDEE 2513

  Fly  1924 ---------GESIAQRCQQLR 1935
                     ..|:|:..:..|
Human  2514 EMNGWLEAVASSVAEHAEIAR 2534

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742	3/51 (6%)
		SPEC	1074..1287	CDD:238103	63/215 (29%)
		SPEC	1182..1425	CDD:238103	57/244 (23%)
		SPEC	1426..1638	CDD:238103	50/245 (20%)
		SPEC	1533..1744	CDD:238103	44/230 (19%)
		SPEC	1646..1850	CDD:238103	36/225 (16%)
		SPEC	1852..2062	CDD:238103	20/104 (19%)
		SPEC	1958..2176	CDD:238103
		SPEC	2072..2296	CDD:238103
		EFh	2311..2379	CDD:238008
		EFhand_Ca_insen	2386..2456	CDD:400872
		SPEC	48..258	CDD:238103	55/221 (25%)
		SPEC	154..364	CDD:238103	53/210 (25%)
		SPEC	261..470	CDD:238103	61/223 (27%)
		SPEC	471..680	CDD:238103	60/208 (29%)
		SPEC	682..893	CDD:238103	63/222 (28%)
		Spectrin	893..>961	CDD:395348	26/67 (39%)
SPTBN4	NP_066022.2	Actin-binding	1..282
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..37
		CH	62..165	CDD:237981
		CH	181..284	CDD:237981
		SPEC	311..419	CDD:295325
		Spectrin 1. /evidence=ECO:0000255	311..418
		SPEC	430..644	CDD:238103
		Spectrin 2. /evidence=ECO:0000255	430..533
		Spectrin 3. /evidence=ECO:0000255	536..641
		Spectrin 4. /evidence=ECO:0000255	774..879		24/113 (21%)
		SPEC	775..987	CDD:238103	55/220 (25%)
		Spectrin 5. /evidence=ECO:0000255	884..982		28/97 (29%)
		Spectrin 6. /evidence=ECO:0000255	1089..1196		36/115 (31%)
		SPEC	1091..1195	CDD:197544	35/112 (31%)
		SPEC	1201..1409	CDD:238103	60/207 (29%)
		SPEC	1306..1514	CDD:238103	59/220 (27%)
		Spectrin 7. /evidence=ECO:0000255	1306..1407		32/100 (32%)
		Spectrin 8. /evidence=ECO:0000255	1412..1512		22/112 (20%)
		Spectrin 9. /evidence=ECO:0000255	1515..1617		36/101 (36%)
		SPEC	1516..1727	CDD:238103	80/284 (28%)
		SPEC	1622..1834	CDD:238103	79/287 (28%)
		Spectrin 10. /evidence=ECO:0000255	1623..1725		41/175 (23%)
		Spectrin 11. /evidence=ECO:0000255	1728..1830		36/103 (35%)
		Spectrin	1834..1933	CDD:278843	26/98 (27%)
		Spectrin 12. /evidence=ECO:0000255	1835..1935		26/99 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1853..1872		4/18 (22%)
		SPEC	1943..>2110	CDD:238103	48/198 (24%)
		Spectrin 13. /evidence=ECO:0000255	1944..2046		29/133 (22%)
		Spectrin 14. /evidence=ECO:0000255	2049..2123		18/73 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2208..2439		51/299 (17%)
		PH	2419..2527	CDD:278594	28/144 (19%)
		PH_beta_spectrin	2421..2525	CDD:269975	25/140 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2533..2564		1/2 (50%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D15759at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100348
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.820