Protein Alignment alpha-Spec and actn4

DIOPT Version :9

• Sequence 1: NP_001261286.1
• Gene: alpha-Spec / 38231
• FlyBaseID: FBgn0250789
• Length: 2457
• Species: Drosophila melanogaster
• Sequence 2: XP_021337009.1
• Gene: actn4 / 322221
• ZFINID: ZDB-GENE-030131-940
• Length: 923
• Species: Danio rerio
• Alignment Length: 932
• Identity: 236/932 - (25%)
• Similarity: 388/932 - (41%)
• Gaps: 285/932 - (30%)

  Fly  1630 KSLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKH--QLVEADIVAHED 1692
            |:...|..|.|..:|:       |              ||..:...|:.:|  :|::.|.:..:|
Zfish   166 KTAPYKNVNVQNFHIS-------W--------------KDGLAFNALIHRHRPELIDYDKLRKDD 209

  Fly  1693 RIKDMNN---------------QADSLVESGQFDT--------------AGIQEKRQSINERYER 1728
            .:.::||               .|:.:|.:.:.|.              :|.|:...:.|    |
Zfish   210 PVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAAN----R 270

  Fly  1729 ICNLAAHRQARLNEALTLHQFFRDIADEESWIK------------------EKKLLVGSDDYGRD 1775
            ||.:.|..|.  ||.| :..:.:..:|...||:                  ::||    :|: ||
Zfish   271 ICKVLAVNQE--NEHL-MEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKL----EDF-RD 327

  Fly  1776 LTGVQNLKKKHKRLEAELG----------SHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKALNQ 1830
            ...|....|..::.:.|:.          |:.||.                 :|...:.:..:|.
Zfish   328 YRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAF-----------------MPSEGRMVSDING 375

  Fly  1831 AWAELKNLAATRG------------QKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGD-SM 1882
            ||.:|:  .|.:|            ::||.  ..::|..:....|.|...|:.:|:.:||.. |:
Zfish   376 AWHKLE--GAEKGYEEWLLNEIRRLERLDH--LAEKFRQKAAIHEGWTDGKEAMLTQKDYETASL 436

  Fly  1883 AAVQGLLKKHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAAR 1947
            :.::.|||||:|||:|..||:||...|.....||.|...:...|:..|||:
Zfish   437 SEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQK-------------- 487

  Fly  1948 RKGALLDNSAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEG 2012
                                                                             
Zfish   488 ----------------------------------------------------------------- 487

  Fly  2013 IHNITALKDQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFA 2077
                                        :..:|..|...:..|::.|...::|...|:||||.:|
Zfish   488 ----------------------------ICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYA 524

  Fly  2078 KKASAFNSWFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALD---QKIKS 2139
            |:|:.||:|.|.|.|||.|....::|:||:.|..||.||:::|..|..:.:|:.|:.   |||.:
Zfish   525 KRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIAN 589

  Fly  2140 FN----VGPNPYTWFTMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQW 2200
            :|    .|.||||..|.:::::.|..:|:::.:||..|.:|..||:.||.||::||..||:...|
Zfish   590 YNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPW 654

  Fly  2201 LTETRYYMLGYNRQ---GTSMMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLIL 2262
            :         .|:.   |...:|.:|:||.||..|......:...:.::.::|....|::|.||.
Zfish   655 I---------QNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIF 710

  Fly  2263 DNRYT----EHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDK 2323
            ||:||    ||..||    |:||.....|..:.:|.|:..|:..|:|::.|.|:...|.|||||.
Zfish   711 DNKYTAYTMEHLRVG----WEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKDH 771

  Fly  2324 SGKLNHQEFKSCLRALGYDLP------------------MVEEGQP--DPEFEAILDVVDPNRDG 2368
            ||.|..:|||:||.:||||:.                  ::..|..  |.||..|:.:||||..|
Zfish   772 SGLLQSEEFKACLISLGYDVENDKQKRTGIMDSDDFRALLISTGNSLGDAEFARIMGIVDPNNSG 836

  Fly  2369 YVSLQEYIAFMISKETENVQSYEEIENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFS 2433
            .|:.|.:|.|| |:||.:..:.:::..:|: |.|.|:.|:|.|||...|..|.|:||:.||.|:|
Zfish   837 AVTFQAFIDFM-SRETTDTDTADQVIASFK-ILAGDKNYITAEELRRELPPDQAEYCIARMAPYS 899

  Fly  2434 EPRSGQPIKDALDYIDFTRTLF 2455
            .|   ..:..||||:.|:..|:
Zfish   900 GP---DAVAGALDYMSFSTALY 918

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742
		SPEC	1074..1287	CDD:238103
		SPEC	1182..1425	CDD:238103
		SPEC	1426..1638	CDD:238103	2/7 (29%)
		SPEC	1533..1744	CDD:238103	28/144 (19%)
		SPEC	1646..1850	CDD:238103	47/274 (17%)
		SPEC	1852..2062	CDD:238103	33/210 (16%)
		SPEC	1958..2176	CDD:238103	49/224 (22%)
		SPEC	2072..2296	CDD:238103	81/237 (34%)
		EFh	2311..2379	CDD:238008	32/87 (37%)
		EFhand_Ca_insen	2386..2456	CDD:400872	25/70 (36%)
		SPEC	48..258	CDD:238103
		SPEC	154..364	CDD:238103
		SPEC	261..470	CDD:238103
		SPEC	471..680	CDD:238103
		SPEC	682..893	CDD:238103
		Spectrin	893..>961	CDD:395348
actn4	XP_021337009.1	SAC6	38..>402	CDD:227401	50/287 (17%)
		SPEC	404..631	CDD:238103	78/333 (23%)
		Spectrin	639..742	CDD:306853	33/115 (29%)
		EFh_PEF	749..>880	CDD:330173	48/132 (36%)
		EFhand_Ca_insen	853..919	CDD:312305	25/70 (36%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D543832at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.920