DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment alpha-Spec and Sptbn4

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	XP_008757363.1	Gene:	Sptbn4 / 308458	RGDID:	1304682	Length:	2609	Species:	Rattus norvegicus

Alignment Length:	1516	Identity:	405/1516 - (26%)
Similarity:	680/1516 - (44%)	Gaps:	179/1516 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 IQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHE--KLQAASEESYRDPTN 80
            :.||......|:...:....::..:|:::|....|..|.|.|..|:.:  :|.||.:..: |..:
  Rat   788 LAERAASARRRWQRLEEAAARRERRLQEARALHQFGADLDGLLDWLRDAYRLAAAGDFGH-DEAS 851

  Fly    81 LQAKIQKHQAFEAEVSAHSNAIVSL----------DNTGQEMINQQHFASESIQVRLDELHKLWE 135
            .:...::|:|...||.||...:..|          ...|..::        ::|||:.|..:|:.
  Rat   852 SRRLARQHRALTGEVEAHRGPVGGLRRQLATLGGASGAGPLVV--------ALQVRVVEAEQLFA 908

  Fly   136 LLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDEFQKD 200
            .:....|.:...|:.||.:.:...:......||.:||.::.|......|:.|||:|.:|:...::
  Rat   909 EVTEVAALRRQWLRDALAVYRMFGEVHACELWIGEKEQWLLAMRVPDSLDDVEVVQHRFESLDQE 973

  Fly   201 MASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQRFNR 265
            |.|...||.:|||...:||:.|||..|.:...::.||..|.|:.:|...|:|::.....::....
  Rat   974 MNSLMGRVLDVNQTVQELVEGGHPSSDEVRSCQDHLNSRWNRIVELVEQRKEEMSAVLLVENHVL 1038

  Fly   266 DADETVAWIAEK-DVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCSIHA 329
            :..|..|.:.|| ..|.|:...|      .|||.:..|:|..|.|||.:.:.|..||..|.....
  Rat  1039 EVAEVRAQVREKRRAVESAPRAG------GALQWRLSGLEAALQALEPRQAALLEEAALLAERFP 1097

  Fly   330 DHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADELAK 394
            ..:.::.....|:...|.:|...|:...:.:..:..|.|||.|....:.|      ::.|.|.|.
  Rat  1098 AQATRLHQGAEELGAEWGALAGAAQACGEAVAAAGRLQRFLRDLDTFLDW------LVRAQEAAG 1156

  Fly   395 DVAG--------AEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAE------IQEKL 445
            .|.|        |:.||.||...|.|:|.||:.:.....:.:.||..:   .||      :.|.|
  Rat  1157 AVEGPLPRSLEEADGLLARHAALKEEVDQREEDYARIVAASEALLASD---GAELGPGLALDEWL 1218

  Fly   446 AALENDKSSLLSLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIK 510
            ..||.....||.|||:||....|....|||.||..||...:..||..|:..:|..:::|||..:|
  Rat  1219 PHLEVGWHKLLGLWEERREALVQAHVYQLFLRDLCQALAVLRNQEVALSGAELPCTVESVEEAMK 1283

  Fly   511 KHEDFEKSLAAQEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRR------ 569
            :|.||..::...::|::.    |.:..:|.....:...::.|..:||   |.|||.:.:
  Rat  1284 RHRDFLTTMELNQQKMQV----AVQAAEGLLRQGNAYGEQAQEAVAR---LLEKSQENQLRAQQW 1341

  Fly   570 -QLLEDSNRYQQFERDCDETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIED 633
             |.|.|....|.|.|||.|..|||.||:..|.|.:..|...|:.:..:||.|..||..||..:|.
  Rat  1342 MQKLLDQLVLQHFLRDCHELDGWIHEKMLMARDGTREDSHKLHKRWLRHQAFMAELAQNKEWLEK 1406

  Fly   634 ITNVGTELIEKQHYAADQINTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLS 698
            |...|.:|::::...|..:..::.||...|..|...:..|..:|.||.:..|..::..:::..|.
  Rat  1407 IEREGQQLMQEKPELAASVRKKLGEIRQCWAELESTTQAKARQLFEASKADQLVQSFAELDKRLL 1471

  Fly   699 EIEGQLLSEDHGKDLTSVQNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEG 763
            .:|.||...|.|.||.:|.:..||...:|:.|......:..:: |....:.......:.:..::.
  Rat  1472 HMESQLQDVDPGGDLATVNSQLKKLQSMESQVEEWCREVGELQ-AQTAALPLEQASKELVGERQS 1535

  Fly   764 NLSARYAALAAPMGERKQHLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKK 828
            .:..|...|..|:.||::.||.|.::.|:..||:||.||::|:.|:|..|.||..|..||..|||
  Rat  1536 AVGERLVRLLEPLQERRRLLLASKELHQVAHDLDDELAWVQERLPLAMLTERGTGLQAVQQHIKK 1600

  Fly   829 HQAVLAEINNHEARLLNVISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSL 893
            :|.:..||..|..||..|:.....:...:...::.:|:..|.||..|..|:|.:.:|:|.||.:.
  Rat  1601 NQGLRREIQAHGPRLEEVLERAGVLASLRSPEAEAVRRGQEQLQSAWTGLREATERRQQTLDAAF 1665

  Fly   894 QAHQYFADANEAESWMREKEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKN 958
            |..||:.|..|.|:|:.|:|.:....|.||||.|:..|||||..|...:|.:..:|..|..|
  Rat  1666 QVEQYYFDVAEVEAWLGEQELLMMSEDKGKDEQSTLQLLKKHLQLEQGVENYEESIAQLSRQ--- 1727

  Fly   959 CRQQETPVVDITGKECVVALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAY 1023
            ||                                     .||...:.|
  Rat  1728 CR-------------------------------------ALLEMGHPD----------------- 1738

  Fly  1024 IKKIDAGLSASQQNLVDNHSIAKRQNQINSQYDNLLALARERQNKLNETVKAYVLVREAADLAQW 1088
                      |:|       |::||:|::..|..|..|..||:..|.:....|.|.|:..:|..|
  Rat  1739 ----------SEQ-------ISRRQSQVDRLYVALKELGEERRVSLEQQYWLYQLSRQVDELEHW 1786

  Fly  1089 IRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDL-KANEVRLANMNEIAVQLTSLGQTEAALKI 1152
            |.:||..|...: :|:|.|.|.|||:||.:|..:. .|...|||.:|::..:|...|.|.||...
  Rat  1787 IAEKEVVAGSPE-LGQDFEHVSVLQEKFSEFASETGTAGRERLAAVNQMVDELIECGHTAAATMA 1850

  Fly  1153 QTQMQDLNEKWNNLQTLTAEKASQLGSAHEVQRFHRDIDETKDWIAEKANALNNDDLGKDLR-SV 1216
            :.: ..|||.|..|..|...:|..|.::.|:.:|..|..|.:..|.||...|.......:.| |.
  Rat  1851 EWK-DGLNEAWAELLELMGTRAQLLAASRELHKFFSDARELQGQIEEKRRRLPRLTAPPEPRPSA 1914

  Fly  1217 QTLQRKHEGVERDLAALRDKIRQLDETANRLMQSHP-DTAEQTYAKQKEINEMWDQIITKSTARK 1280
            .::||.....|.||..|..::|||.|.|.:|...:. :.||...::::|:.:.|.:::......:
  Rat  1915 SSMQRTLRAFEHDLQLLVSQVRQLQEGAAQLRTVYAGEHAEAIASREQEVLQGWKELLAACEDAR 1979

  Fly  1281 EKLLDSYDLQRFLSDYRDLLAWINSMMSLVTSDELANDVTGAEALIERHQARRAEIGFTLGISSA 1345
            ..:..:.|..||.|..||||:|::.:...:.:.:...||:..|.|:..||          |:
  Rat  1980 LHVSSTADALRFHSQARDLLSWMDGIAGQIGAADKPRDVSSVEVLMNYHQ----------GL--- 2031

  Fly  1346 PGAAASTSSIASPSGDEEHRTEIDARAGTFGAFEQFGNELLQANHYASPEIKEKIEDLAKAREDL 1410
                               :||::||.....|.::.|..||......:.||:.:::.|...:|::
  Rat  2032 -------------------KTELEARVPELTACQELGRSLLLNKSAMADEIQAQLDKLGSRKEEV 2077

  Fly  1411 EKAWTERRLQLEQNLDLQLYMRDCELAESWMSAREAFLNADDDANAGGNVEALIKKHEDFDKAIN 1475
            .:.|......|:|.|::..:.::..:|::|::|:|..|.:.:..::...||.||::||.|.||..
  Rat  2078 SEKWDRHWEWLQQMLEVHQFAQEAVVADAWLTAQEPLLQSRELGSSVDEVEQLIRRHEAFRKAAA 2142

  Fly  1476 GHEQKIAALQ--TVADQLIAQ 1494
            ..|::.::|:  |..::|.|:
  Rat  2143 AWEERFSSLRRLTTIEKLKAE 2163

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742	3/51 (6%)
		SPEC	1074..1287	CDD:238103	63/215 (29%)
		SPEC	1182..1425	CDD:238103	57/244 (23%)
		SPEC	1426..1638	CDD:238103	19/71 (27%)
		SPEC	1533..1744	CDD:238103
		SPEC	1646..1850	CDD:238103
		SPEC	1852..2062	CDD:238103
		SPEC	1958..2176	CDD:238103
		SPEC	2072..2296	CDD:238103
		EFh	2311..2379	CDD:238008
		EFhand_Ca_insen	2386..2456	CDD:400872
		SPEC	48..258	CDD:238103	57/221 (26%)
		SPEC	154..364	CDD:238103	55/210 (26%)
		SPEC	261..470	CDD:238103	61/223 (27%)
		SPEC	471..680	CDD:238103	64/215 (30%)
		SPEC	682..893	CDD:238103	60/210 (29%)
		Spectrin	893..>961	CDD:395348	26/67 (39%)
Sptbn4	XP_008757363.1	CH	111..214	CDD:237981
		CH	230..333	CDD:237981
		SPEC	361..584	CDD:238103
		SPEC	479..693	CDD:238103
		SPEC	819..1031	CDD:238103	57/220 (26%)
		SPEC	1135..1239	CDD:197544	35/112 (31%)
		SPEC	1245..1453	CDD:238103	64/214 (30%)
		SPEC	1351..1558	CDD:238103	57/207 (28%)
		SPEC	1560..1771	CDD:238103	79/284 (28%)
		SPEC	1666..1878	CDD:238103	79/287 (28%)
		Spectrin	1878..1977	CDD:278843	26/98 (27%)
		SPEC	1987..>2154	CDD:238103	48/198 (24%)
		PH	2464..2566	CDD:278594
		PH_beta_spectrin	2466..2570	CDD:269975
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D15759at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100348
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.820