DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment alpha-Spec and Sptan1

DIOPT Version :9

Sequence 1:	NP_001261286.1	Gene:	alpha-Spec / 38231	FlyBaseID:	FBgn0250789	Length:	2457	Species:	Drosophila melanogaster
Sequence 2:	XP_006497872.1	Gene:	Sptan1 / 20740	MGIID:	98386	Length:	2510	Species:	Mus musculus

Alignment Length:	2534	Identity:	1595/2534 - (62%)
Similarity:	1949/2534 - (76%)	Gaps:	112/2534 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 ENFTPKEVKILETVEDIQERREQVLSRYNDFKIETRQKREKLEDSRRFQYFKRDADELESWIHEK 66
            :...|..||:|||.|||||||:|||.||:.||..:..:|:|||||.|||:|:|||:|||.||.||
Mouse    11 QKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEK 75

  Fly    67 LQAASEESYRDPTNLQAKIQKHQAFEAEVSAHSNAIVSLDNTGQEMINQQHFASESIQVRLDELH 131
            ||.||:|:|:||||||.|:||||||||||.|:|.|||.||.||..||::.|||||:|:.||.|||
Mouse    76 LQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELH 140

  Fly   132 KLWELLLSRLAEKGLKLQQALVLVQFLRQCEEVMFWIKDKETFVTADEFGQDLEHVEVLQRKFDE 196
            :.|||||.::.|||:||.||..|||:||:||:||.||.|||..||::|.|||||||||||:||:|
Mouse   141 RQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEE 205

  Fly   197 FQKDMASQEYRVTEVNQLADKLVQDGHPERDTITKRKEELNEAWQRLKQLAIVRQEKLFGAHEIQ 261
            ||.|:|:.|.||.||:|.|.||:|:.|||.:.|..:::|:|.||||||.||:.||.|||||.|:|
Mouse   206 FQTDLAAHEERVNEVSQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQ 270

  Fly   262 RFNRDADETVAWIAEKDVVLSSDDYGRDLASVQALQRKHEGVERDLAALEDKVSTLGAEAQRLCS 326
            |||||.|||:.||.||:.:::|||:||||||||||.|||||:|||||||||||..|.|||.||..
Mouse   271 RFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQ 335

  Fly   327 IHADHSDQIRDKQAEIANYWQSLTTKARERKQKLDESYYLHRFLADFRDLVSWINGMKAIISADE 391
            .|...:.||:.|:.|:...|:.:.|.|.||..:||:||.|.|||||||||.||:..|||:|:|||
Mouse   336 SHPLSASQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADE 400

  Fly   392 LAKDVAGAEALLERHQEHKGEIDAREDSFKLTTESGQKLLEREHYAAAEIQEKLAALENDKSSLL 456
            ||.|||||||||:|||||||||||.|||||...||||.||...|||:.|::|||:.|..::::||
Mouse   401 LANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAASHYASDEVREKLSILSEERTALL 465

  Fly   457 SLWEDRRILYEQCMDLQLFYRDTEQADTWMAKQEAFLANEDLGDSLDSVEALIKKHEDFEKSLAA 521
            .|||.||..||||||||||||||||.|.||:||||||.||||||||||||||:||||||||||:|
Mouse   466 ELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSA 530

  Fly   522 QEEKIKALDIFATKLIDGQHYAADDVAQRRQMLLARRAALQEKSSKRRQLLEDSNRYQQFERDCD 586
            |||||.|||.||||||...|||.:|||.||..||:||.||.|::..||..|.||...|||.||.|
Mouse   531 QEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFRDSD 595

  Fly   587 ETKGWISEKLKFATDDSYLDPTNLNGKMQKHQNFEHELNANKSRIEDITNVGTELIEKQHYAADQ 651
            |.|.|::||:|.|||::|.||:||.||:||||.||.||:||:|||:.:...|.:||:..|||.::
Mouse   596 ELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKEE 660

  Fly   652 INTRMQEIVVLWETLVQASDKKGTKLNEACQQQQFNRTIEDIELWLSEIEGQLLSEDHGKDLTSV 716
            :..||.|::.||:.|::|::.||.||.||.|||||||.:|||||||.|:||.|.|:|:|||||:|
Mouse   661 VAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNV 725

  Fly   717 QNLQKKHALLEADVMAHQDRIESIKVAANKFIESGHFDADNIRNKEGNLSARYAALAAPMGERKQ 781
            ||||||||||||||.||||||:.|.:.|.:|.::|||||:||:.|:..|.|||.||..||..|||
Mouse   726 QNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQ 790

  Fly   782 HLLDSLQVQQLFRDLEDEAAWIREKEPIAASTNRGRDLIGVQNLIKKHQAVLAEINNHEARLLNV 846
            .|.|||::||||||:|||..|||||||||||||||:||||||||:|||||:.|||..||.|:..|
Mouse   791 KLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAV 855

  Fly   847 ISSGENMLKDQPFASDDIRQRLEALQEQWNTLKEKSSQRKQDLDDSLQAHQYFADANEAESWMRE 911
            ...|..|:::..||::|::.:|..|.::|..||.|:|||:|||:|||||.|||||||||||||||
Mouse   856 TQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMRE 920

  Fly   912 KEPIATGSDYGKDEDSSEALLKKHEALVSDLEAFGNTIQALQEQAKNCRQQETPVVDITGKECVV 976
            ||||...:||||||||:||||||||||:|||.|:|::||||:|||::||||..|:.|.||||.|:
Mouse   921 KEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVL 985

  Fly   977 ALYDYTEKSPREVSMKKGDVLTLLNSNNKDWWKVEVNDRQGFVPAAYIKKIDAGLSASQQNLVDN 1041
            |||||.|||||||:|||||:||||||.||||||||||||||||||||:||:|...|||::||::.
Mouse   986 ALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEE 1050

  Fly  1042 H-SIAKRQNQINSQ--------------------YDNLLALARERQNKLNETVKAYVLVREAADL 1085
            . |||.||.||::|                    |.:||.|..:|:..|.::.|.::|.|||.:|
Mouse  1051 QGSIALRQGQIDNQTRITKEAGSVSLRMKQVEELYQSLLELGEKRKGMLEKSCKKFMLFREANEL 1115

  Fly  1086 AQWIRDKENHAQIADVVGEDLEEVEVLQKKFDDFNDDLKANEVRLANMNEIAVQLTSLG------ 1144
            .|||.:|| .|...:.||.|||:|||||||||||..||||||.||.::|::|..|.|.|
Mouse  1116 QQWITEKE-AALTNEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEV 1179

  Fly  1145 ----QTE--------------------AALKIQT-----QMQDLNEKWNNLQTLTAEKASQLGSA 1180
                |.|                    |.|.:.|     .:::|||:|.:||.|..|::..||||
Mouse  1180 QAVQQQEVYGAMPRDEADSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSA 1244

  Fly  1181 HEVQRFHRDIDETKDWIAEKANALNNDDLGKDLRSVQTLQRKHEGVERDLAALRDKIRQLDETAN 1245
            |||||||||.||||:||.||..|||.|:.|.||.|||.|||||||.|||||||.||:..|.|||.
Mouse  1245 HEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAQ 1309

  Fly  1246 RLMQSHPDTAEQTYAKQKEINEMWDQIITKSTARKEKLLDSYDLQRFLSDYRDLLAWINSMMSLV 1310
            ||:||||::||....|..|:|:.|..:..::..||.||.||:||||||||:|||::|||.:..||
Mouse  1310 RLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLV 1374

  Fly  1311 TSDELANDVTGAEALIERHQARRAEIGFTLGISSAPGAAASTSSIASPSGDEEHRTEIDARAGTF 1375
            :|||||.||||||||:||||                                |||||||||||||
Mouse  1375 SSDELAKDVTGAEALLERHQ--------------------------------EHRTEIDARAGTF 1407

  Fly  1376 GAFEQFGNELLQANHYASPEIKEKIEDLAKAREDLEKAWTERRLQLEQNLDLQLYMRDCELAESW 1440
            .||||||.:||...||||||||||::.|.:.|.||||||.:||:.|:..|:|||:.||||.||:|
Mouse  1408 QAFEQFGQQLLAHGHYASPEIKEKLDILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENW 1472

  Fly  1441 MSAREAFLNADDDANAGGNVEALIKKHEDFDKAINGHEQKIAALQTVADQLIAQNHYASNLVDEK 1505
            |:|||||||.:|..::..:||||||||||||||||..|:||||||..||||||.:|||...:..:
Mouse  1473 MAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAVDHYAKGDIANR 1537

  Fly  1506 RKQVLERWRHLKEGLIEKRSRLGDEQTLQQFSRDADEIENWIAEKLQLATEESYKDPANIQ---- 1566
            |.:||:|||.||..:|||||:||:.|||||||||.||||.||:||||.|::||||||.|||
Mouse  1538 RNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKL 1602

  Fly  1567 -SKHQKHQAFEAELAANADRIQSVLAMGGNLIDKKQCSGSEDAVQKRLTQIADQWEYLTHKTTEK 1630
             |||||||||||||.||||||:.|:.||.:||::..|:||||||:.||..:||||::|..|:.||
Mouse  1603 LSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEK 1667

  Fly  1631 SLKLKEANKQRTYIAAVKDLDFWLGEVESLLTTEDSGKDLASVQNLMKKHQLVEADIVAHEDRIK 1695
            |.||||||||:.:...:||.||||.|||:||.:||.|||||||.||:|||||:||||.|||||:|
Mouse  1668 SQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLK 1732

  Fly  1696 DMNNQADSLVESGQFDTAGIQEKRQSINERYERICNLAAHRQARLNEALTLHQFFRDIADEESWI 1760
            |:|:|||||:.|..|||:.::|||.:||.|:::|.::|..|:|:|:|:..|||||||:.||||||
Mouse  1733 DLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHRLHQFFRDMDDEESWI 1797

  Fly  1761 KEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRL 1825
            |||||||.|:||||||||||||:||||||||||.:||||||.|.:.|:||.|.:.:|..||:|||
Mouse  1798 KEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGQEEIQQRL 1862

  Fly  1826 KALNQAWAELKNLAATRGQKLDESLTYQQFLAQVEEEEAWITEKQQLLSVEDYGDSMAAVQGLLK 1890
            ....:.|.|||.|||.|||:|:|||.||||:|.||||||||.||..|::.|||||::||:|||||
Mouse  1863 AQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLK 1927

  Fly  1891 KHDAFETDFTAHKDRCSLICDQGSELVEAKNHHGESIAQRCQQLRLKLDNLSALAARRKGALLDN 1955
            ||:|||||||.||||.:.:|..|.:|::..|||.|:|:.:.:.|..|:.:|...||:||..|.:|
Mouse  1928 KHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDEN 1992

  Fly  1956 SAYLQFMWKADVVESWIDDKENYVRSDEFGRDLSTVQTLLTKQETFDAGLNAFEQEGIHNITALK 2020
            ||:|||.||||||||||.:|||.:::|::|||||:|||||||||||||||.||:||||.||||||
Mouse  1993 SAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALK 2057

  Fly  2021 DQLINASHAQSPAILKRHGDVIARWQKLRDASNTRKDRLLAMQEQFRQIEELYLTFAKKASAFNS 2085
            |||:.|.|.||.||..||..::.||.:|...|.|||.:||..|..||::|:|:||||||||||||
Mouse  2058 DQLLAAKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNS 2122

  Fly  2086 WFENAEEDLTDPVRCNSIEEIRALRDAHAQFQASLSSAEADFKALAALDQKIKSFNVGPNPYTWF 2150
            |||||||||||||||||:|||:|||:||..|::|||||:|||..||.||::||||.|..||||||
Mouse  2123 WFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWF 2187

  Fly  2151 TMEALEETWRNLQKIIEERDGELAKEAKRQEENDKLRKEFAKHANLFHQWLTETRYYML---GYN 2212
            ||||||||||||||||:||:.||.||.:|||||||||:|||:|||.||||:.|||.|:|   .|.
Mouse  2188 TMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYR 2252

  Fly  2213 R-------------QGTS-MMEGSGSLEQQLEALRVKATEVRARRVDLKKIEELGALLEEHLILD 2263
            |             .|.| |:|.||:||.||||.:.|..|:||.|..|||||:|||.:||.||||
Mouse  2253 RVIRVCQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILD 2317

  Fly  2264 NRYTEHSTVGLAQQWDQLDQLSMRMQHNLEQQIQARNHSGVSEDSLKEFSMMFKHFDKDKSGKLN 2328
            |:|||||||||||||||||||.|||||||||||||||.:||:|::|||||||||||||||||:||
Mouse  2318 NKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLN 2382

  Fly  2329 HQEFKSCLRALGYDLPMVEEGQPDPEFEAILDVVDPNRDGYVSLQEYIAFMISKETENVQSYEEI 2393
            |||||||||:|||||||||||:||||||||||.|||||||:||||||:|||||:|||||:|.|||
Mouse  2383 HQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEI 2447

  Fly  2394 ENAFRAITAADRPYVTKEELYCNLTKDMADYCVQRMKPFSEPRSGQPIKDALDYIDFTRTLFQN 2457
            |:||||:::..:|||||||||.|||::.|||||..|||:.:.: |:.:..|.||::|||:||.|
Mouse  2448 ESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGK-GRELPTAFDYVEFTRSLFVN 2510

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Spec	NP_001261286.1	SH3_Alpha_Spectrin	974..1026	CDD:212742	44/51 (86%)
		SPEC	1074..1287	CDD:238103	122/247 (49%)
		SPEC	1182..1425	CDD:238103	139/242 (57%)
		SPEC	1426..1638	CDD:238103	141/216 (65%)
		SPEC	1533..1744	CDD:238103	137/215 (64%)
		SPEC	1646..1850	CDD:238103	132/203 (65%)
		SPEC	1852..2062	CDD:238103	129/209 (62%)
		SPEC	1958..2176	CDD:238103	155/217 (71%)
		SPEC	2072..2296	CDD:238103	170/240 (71%)
		EFh	2311..2379	CDD:238008	61/67 (91%)
		EFhand_Ca_insen	2386..2456	CDD:400872	39/69 (57%)
		SPEC	48..258	CDD:238103	136/209 (65%)
		SPEC	154..364	CDD:238103	127/209 (61%)
		SPEC	261..470	CDD:238103	132/208 (63%)
		SPEC	471..680	CDD:238103	131/208 (63%)
		SPEC	682..893	CDD:238103	134/210 (64%)
		Spectrin	893..>961	CDD:395348	53/67 (79%)
Sptan1	XP_006497872.1	Spectrin	56..158	CDD:334073	68/101 (67%)
		SPEC	162..373	CDD:238103	127/210 (60%)
		SPEC	374..584	CDD:238103	149/209 (71%)
		SPEC	480..690	CDD:238103	132/209 (63%)
		SPEC	691..902	CDD:238103	134/210 (64%)
		SPEC	905..>983	CDD:197544	58/77 (75%)
		SH3_Alpha_Spectrin	983..1035	CDD:212742	44/51 (86%)
		SPEC	1104..1351	CDD:238103	122/247 (49%)
		SPEC	1246..1457	CDD:238103	139/242 (57%)
		SPEC	1458..1675	CDD:238103	141/216 (65%)
		Spectrin	1675..1777	CDD:334073	61/101 (60%)
		SPEC	1782..1991	CDD:238103	131/208 (63%)
		SPEC	1888..2099	CDD:238103	129/210 (61%)
		SPEC	1995..2213	CDD:238103	155/217 (71%)
		SPEC	2113..2350	CDD:238103	167/236 (71%)
		EFh	2365..2433	CDD:238008	61/67 (91%)
		EFhand_Ca_insen	2440..2508	CDD:312305	38/68 (56%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850184
Domainoid	1	1.000	171	1.000	Domainoid score	I3745
eggNOG	1	0.900	-	-		E1_KOG0040
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2353
Inparanoid	1	1.050	3017	1.000	Inparanoid score	I15
Isobase	1	0.950	-	0	Normalized mean entropy	S2319
OMA	1	1.010	-	-		QHG51850
OrthoDB	1	1.010	-	-		D15759at33208
OrthoFinder	1	1.000	-	-		FOG0004911
OrthoInspector	1	1.000	-	-		oto95176
orthoMCL	1	0.900	-	-		OOG6_100348
Panther	1	1.100	-	-	LDO	PTHR11915
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3104
SonicParanoid	1	1.000	-	-		X5245
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	18	17.750