Protein Alignment pns and lst-6

DIOPT Version :10

• Sequence 1: NP_647620.1
• Gene: pns / 38178
• FlyBaseID: FBgn0035229
• Length: 1449
• Species: Drosophila melanogaster
• Sequence 2: NP_001367509.1
• Gene: lst-6 / 174146
• WormBaseID: WBGene00016889
• Length: 1487
• Species: Caenorhabditis elegans
• Alignment Length: 1611
• Identity: 511/1611 - (31%)
• Similarity: 750/1611 - (46%)
• Gaps: 379/1611 - (23%)

  Fly    74 DYFVICGLDLDTGLEPD-RFAGDNLHCS-----------PLDRAYKSKPLAHYPENVPWNPFDAH 126
            |||.:.|.|.|.||..| ::...:..|.           ||.|::.:|.:.|:|:..|.||| :.
 Worm    14 DYFAVAGYDPDIGLVVDTKYDPGHTPCESPGSSAPEFRPPLQRSFVAKIINHFPQRRPGNPF-SD 77

  Fly   127 GICMLSLPQGLRFRTQKHCIEPR---FHSFATTREDGKRCYGFSLVFYEEIRNRNICGAIHTLQS 188
            .|..|.:|:||||.|:|..  |:   .|:||..:|||.|..|.:|.||||:::..||.|:..||.
 Worm    78 DILHLCMPRGLRFYTEKDV--PKKLMIHTFANIKEDGSRINGTALTFYEEVKDLAICDAMAQLQQ 140

  Fly   189 MFITELS---NG----QQ------------THSLSRVKQDGPVSRSLPRHFKVAGQAPQSAQSYY 234
            ..:.|::   ||    ||            ||:|.|.::|        |..::         |||
 Worm   141 DHVREITARHNGIIEKQQRIHHPPGTVSGGTHTLPRNRRD--------RSKRI---------SYY 188

  Fly   235 D-INKDKLYVAKSITLICQAPYAFAAQMFLKNLYRCLPRQPGAGISLESYVYNILYEVMLPQPGK 298
            : ...:.||::::|.::.:.|...:.:..|..|::.:.......:.||||:|..||||.||.|..
 Worm   189 EGGGHNSLYMSRTICVLSRLPLVNSTRALLTALHQIIASPSSPHLPLESYIYWCLYEVPLPSPSM 253

  Fly   299 SIRVYLPPTEPHLPPIALILQRPLLATELPLLDFPLRLLFKYLGVECVIQLLTCVLLENQVLLRS 363
            ::::.|..|       .|::||| ...|||..|..:..:|.||.||..|:|.:|.|||:|:||.|
 Worm   254 TLKIPLLGT-------TLVVQRP-GPKELPFFDDSIGSIFDYLPVEKFIRLFSCFLLEHQILLCS 310

  Fly   364 TDYQRLMIVGECITSLLFPFVWPHVYAPILPAALHHFLDAPVPFVMGLHAECEAAHKIGSEATLC 428
            .|..|||.|.|.:.:|.|||.|...|||:||.:...|.:||||:|||.:.|.....:: .::.:|
 Worm   311 KDLSRLMTVCESLLALSFPFRWQMTYAPVLPYSKLDFFEAPVPYVMGWYYEDTVPEEL-FQSNVC 374

  Fly   429 FVDIDKKHIQLPEELPVFPHKVDFMAEIGSVLDKFE---------IERDRDQEPSFRNGYVGRGA 484
            .||:|....:|||::||||.......:|.:.|::..         ::|...:|.          .
 Worm   375 VVDLDTGRSELPEDVPVFPGARQLATDIKNSLERLSDSDKMLTSTLDRSEPEEV----------V 429

  Fly   485 GGGVSGSGSSGSGGVDAMISSCTLPTGLQAARRSK----ERFQQMQETVYTLSGSPGGGVVDYQP 545
            .|||.            |......|....|.|.|:    |....|.|   .|||
 Worm   430 VGGVK------------MRRKNKRPEDWAAKRMSRSFDLEDGSAMSE---ELSG----------- 468

  Fly   546 LIAHPSRIDHVP--------------RIADFLRRKGGIRGAGSPVGSPTVSLSSSPVGIYHDVDA 596
            ::..|||....|              |:|:..||                      .|:..|||.
 Worm   469 MMRRPSRTGLQPLPLENVLRNNSTLARVAEIARR----------------------AGVVVDVDN 511

  Fly   597 VDATMPVSTRRAEKQKL-SPVDQ-YYQDLRINNSVREIFLNRFVHMFHAYEYFVIYPN--QARDE 657
            :.:..      |:.|.| ||:.: |:.|.::||::||..|||||.||::||:|||...  ..|:.
 Worm   512 IKSEF------ADAQNLDSPIARHYFVDAKVNNAIRECVLNRFVEMFYSYEHFVIGGQGCDDRES 570

  Fly   658 WISNRETLQNFDKSSFLSDQPEHHRAFLSRFLESQMFATLIDNKILAMWESQPDEQLQLFDQRVK 722
            :.:|||::.:|||:|||||||:.|..||:.|||:|||.:.||:|||:.||: |||.:.|||.|:.
 Worm   571 YEANRESMADFDKASFLSDQPDSHLTFLAAFLETQMFTSFIDSKILSQWET-PDEGVVLFDSRIS 634

  Fly   723 LLRRRHGENMIAATSYE---PCVLSQDSQQGFEKRLNCIDIEVTPPSEILANRAAYFRSFPLLEK 784
            .:|...|.:::...:||   |...::|.   ..||....|. |.|....||.      :||:..:
 Worm   635 AMRDLLGVSIVRTPTYEATPPFAATEDL---ISKREETTDY-VVPVPHTLAG------AFPVRYE 689

  Fly   785 GV-----LNQECASRGNSLRRVKNGNKWRAREISL-DQKPGSNAINRLSANLTTADVSPAL---- 839
            |.     ||.......|.:....  :.||.|...| .||.|||..:..|...|:......|
 Worm   690 GAWPVHQLNTTLLDGANLISPAP--SPWRQRYPRLRSQKDGSNGNSGQSVRPTSVYGGAGLMQVE 752

  Fly   840 ----IAQANWTFVERLLKDIKSKTKRMLLEKMGNEAVALGLKG---DGIEENTLIASMCDLLEKI 897
                |||..:.||::|||:.|:||||||::|||.|||.||...   .|:|||||:||.|||||:|
 Worm   753 SSKQIAQQQYAFVQQLLKETKAKTKRMLVDKMGKEAVQLGHLDAGITGVEENTLVASFCDLLERI 817

  Fly   898 WSHGLQNKQGKSALWAHLQAYLEMQEARGAGDSSGTEQ-TP------------------------ 937
            |:|||:||.|||:||..:..:.:.::......:|.|.. ||
 Worm   818 WAHGLKNKHGKSSLWNFVLQHQDFEKPGLTTRASSTSMLTPAIRGFRAPSLPPAPQSTHNRRGGA 882

  Fly   938 -------PVSSSPGGKMTIATA------------------------------------------- 952
                   |:..||.....:.||
 Worm   883 PIDEVPAPIILSPQNNTDMLTAISDIVDSFRGAQEEPWSKSLLRAATSIVEKYKNIEKDSNGNPL 947

  Fly   953 -------------SPALAWNAMRKRMDYLSTF--------------------------QTD---- 974
                         .|::|    |.|...:..|                          |||
 Worm   948 ETNSTLPSRYPITHPSIA----RARNQSVEPFGLQISGKSMKKSSSIGDFTNPAWGGSQTDVSNL 1008

  Fly   975 -----FDSPPSPNRSRSRDRNKFVGLEQLCPLPESLEFDVKNVLAMADIKTHIGYTRAWVRLSLE 1034
                 .:..|...||:||.|:.......|.|||..:.:|:||||.|.:|||.|||.||:|||:||
 Worm  1009 GGYASVEGSPRKMRSQSRTRSPDGARVVLSPLPTHVAYDLKNVLRMTEIKTDIGYARAFVRLALE 1073

  Fly  1035 KKLLSRHFRTLLSDEALLRSLYKRSAFLRCEEEKEQFLYHLLTLNTVDYFSFTNIYPTTKLPYRV 1099
            :|||.:|...|||:..:||.|||..||:..:|||||||:|:|:||...:..|||.:..||:.|:|
 Worm  1074 RKLLHKHLGALLSNSRVLRELYKPYAFVGSDEEKEQFLFHILSLNAAQFRCFTNTFTKTKMDYQV 1138

  Fly  1100 VIFPSRKYGSYHTSSNVWIMVSGTMNETQRVPVPKGSLEFIFHYKNLGLLTTMRIGHDNSGPSHK 1164
            ||......|:...   :|:.|.|::..|..:.:...:..|.|.:||||:|:|:||||..|....:
 Worm  1139 VIVTGSGRGAIPA---IWVTVEGSLCSTPPIMLKPNTPLFKFDHKNLGILSTLRIGHQQSEKPVQ 1200

  Fly  1165 WLVEQVVMRNEVTGHTYKFPCG------RWLGRGVDDDSTERLLVGQ----------------RV 1207
            |.:|.|::|||:||.||||||.      ||.|.| :|.:.||:||.:                ..
 Worm  1201 WFLEYVLVRNEITGQTYKFPCSKKFGEDRWFGNG-EDITLERMLVAEPFVEYDGNDNGIIEQGEP 1264

  Fly  1208 LTNAKNAELVPGSDTRTPPRTRSP-------SVQRQETLAPSEIQHQLGNCVNVLVKWHYKPSRD 1265
            .:.|:...:  .|.::||.|.|||       |..|......||::|.||..||.|||  |..|..
 Worm  1265 FSPARTRRM--SSRSQTPQRERSPSNGRMAESSSRNRKTKVSEVEHLLGEAVNALVK--YFCSER 1325

  Fly  1266 RDVGTLTNLLCGDDGLVKCLEQVFLCGFRSA--RFFGRNLYIWDYFTKI-KELFEHNLQMELEDS 1327
            :....|.:||||..|||..:||.|..|.:.:  ::| ||...|||..:: ...||...:.|::..
 Worm  1326 KVKSELAHLLCGQKGLVLAIEQAFQLGRQESLLKYF-RNTCPWDYIERVCSWFFELCRKKEVDKL 1389

  Fly  1328 SSSLDSSFSNGSGSGGNSLQRREISSIWRLYVQLMDEINGTALGKDGKFQLLICLSLREHLLTRL 1392
            ...                |:..|....|||.::..:   |:|||||||.:.:.||:|:|:|..|
 Worm  1390 PKE----------------QKTMIHHALRLYRKIDAK---TSLGKDGKFHVFVLLSIRDHILPGL 1435

  Fly  1393 IKPMALTKVTHEMYEEESFLRRRNLLTFLIQILEPLDDCHIVLENSITQGI 1443
            :..|..:.||.:||.|.||||....:|:|.:::..|.:.:|.::.|:|.||
 Worm  1436 LPLMRYSPVTADMYNEPSFLRTPAHMTYLAKLMYSLSEFNITIDPSLTYGI 1486

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
pns	NP_647620.1	uDENN	70..171	CDD:214824	39/111 (35%)
		DENN	243..435	CDD:214823	70/191 (37%)
		dDENN	626..701	CDD:129037	41/76 (54%)
		RUN1_DENND5	839..1091	CDD:439039	123/385 (32%)
		PLAT_RAB6IP1	1095..1203	CDD:238855	43/113 (38%)
		RUN2_DENND5	1243..1440	CDD:439040	66/199 (33%)
lst-6	NP_001367509.1	uDENN	11..123	CDD:214824	39/111 (35%)
		DENN	199..381	CDD:214823	70/190 (37%)
		dDENN	537..614	CDD:129037	41/76 (54%)
		RUN	757..1130	CDD:459241	122/376 (32%)
		PLAT_RAB6IP1	1134..1244	CDD:238855	43/113 (38%)
		RUN2_DENND5	1305..1483	CDD:439040	66/199 (33%)