Protein Alignment Myo61F and MYO5B

DIOPT Version :10

• Sequence 1: NP_476934.2
• Gene: Myo61F / 38153
• FlyBaseID: FBgn0010246
• Length: 1052
• Species: Drosophila melanogaster
• Sequence 2: NP_001073936.1
• Gene: MYO5B / 4645
• HGNCID: 7603
• Length: 1848
• Species: Homo sapiens
• Alignment Length: 1081
• Identity: 354/1081 - (32%)
• Similarity: 543/1081 - (50%)
• Gaps: 177/1081 - (16%)

  Fly    15 TKDYIDFNSQLKM----ETGLH-----ERDR----------AGVQDFVLLENYQSEEAFIGNLKK 60
            ||||.:.:..|::    ||.|.     :|::          .|..|...| :|..|.|.:.|||.
Human    28 TKDYKEGDKSLQLRLEDETILEYPIDVQRNQLPFLRNPDILVGENDLTAL-SYLHEPAVLHNLKV 91

  Fly    61 RFQE-DLIYTYIGQVLISVNPYKQLPIYTDDHVKAYRNKHFYEMPPHIFAVTDNAFRSLIEENRG 124
            ||.| :.||||.|.||:::|||:|||||..|.:..|..::..:|.||||||.:.|::.:..:.:.
Human    92 RFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYTYSGQNMGDMDPHIFAVAEEAYKQMARDEKN 156

  Fly   125 QCVLISGESGSGKTEASKKVLQFIAACSG--NQTTVEGVKDKLLKSNPVLEAFGNAKTNRNDNSS 187
            |.:::|||||:|||.::|..:::.|...|  ::|.:|   :|:|.|:|::||.|||||.||||||
Human   157 QSIIVSGESGAGKTVSAKYAMRYFATVGGSASETNIE---EKVLASSPIMEAIGNAKTTRNDNSS 218

  Fly   188 RFGKYMDIQFDFKGAPIGGNILNYLLEKSRVVAQMGGERNFHIFYQLLAGADEALLQELRLERAL 252
            |||||:.|.||.:...||.|:..|||||||||.|...|||:||||||.|.|.....:||.|..|.
Human   219 RFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAAGLPEFKELALTSAE 283

  Fly   253 DTYSYLTDGLNGTVTRINDADSFKQVQQALTVIDFTKEEQREIFGIVASILHLGNVGF-TEVEGN 316
            | :.|.:.|.:.::..::||:.|::.:||.|::...:..|..||.|:|||||||:|.. .|.:|:
Human   284 D-FFYTSQGGDTSIEGVDDAEDFEKTRQAFTLLGVKESHQMSIFKIIASILHLGSVAIQAERDGD 347

  Fly   317 A-KVNSRDLVVT-AARLLGVNASELEAALTHRTIDARGDVVTSPLNQELAIYARDALAKAVYDRL 379
            : .::.:|:.:: ..|||||..|::|..|.||.:....:.....::.:..|.||:||||.:|.:|
Human   348 SCSISPQDVYLSNFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMSLQQVINARNALAKHIYAQL 412

  Fly   380 FSWLVQRLNISLQAKETRASRNNVMGILDIYGFEIFQKNSFEQFCINFCNEKLQQLFIELTLKSE 444
            |.|:|:.:|   :|..|...:::.:|:|||||||.|:.|||||||||:.||||||.|.....|.|
Human   413 FGWIVEHIN---KALHTSLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLE 474

  Fly   445 QDEYRREGIEWIPVEYFDNKVICNLIEEKHKGIISILDEECLRPGEPTDKTFLEKLTQKLAQHHH 509
            |:||.:|.|.|..::::||:...:|||.| .||:.:|||||..| :.||:.:.:||..:.:...|
Human   475 QEEYMKEQIPWTLIDFYDNQPCIDLIEAK-LGILDLLDEECKVP-KGTDQNWAQKLYDRHSSSQH 537

  Fly   510 YVCHEKAPAHIKKIMLRDEFRLVHYAGEVTYSVNGFLDKNNDL-------------------LFR 555
            :.         |..|....|.:||:|.:|.|..:|||:||.|.                   ||.
Human   538 FQ---------KPRMSNTAFIIVHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFH 593

  Fly   556 DLKETL--SKAGNG-----IVRNCFPEKELRSLKRPETAITQFRASLNNLMDILMCKEPSYIRCI 613
            |.|:.:  :..|.|     .||:..|..::.:.:..:|...|||.||:.||:.|....|.|:|||
Human   594 DDKDPVPATTPGKGSSSKISVRSARPPMKVSNKEHKKTVGHQFRTSLHLLMETLNATTPHYVRCI 658

  Fly   614 KPNDLQTANVFNDELVLHQVKYLGLMENLRVRRAGFAYRRTYELFLERYKSLSKSTWPNYKGPGG 678
            ||||.:....|:.:..:.|::..|::|.:|:..||:..|..|..|..||:.|.|.   .......
Human   659 KPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVKK---RELANTD 720

  Fly   679 PKAGVQQLVKDLGWDEEKYRVGETKLFIRWPRTLFDTEDAYQEK----KHEIAAI-IQAHWKGLM 738
            .||..:.::::|..|.:|::.|.||:|.|..:.      ||.||    |...|.| ||...:|.:
Human   721 KKAICRSVLENLIKDPDKFQFGRTKIFFRAGQV------AYLEKLRADKFRTATIMIQKTVRGWL 779

  Fly   739 QRRKYLKLRAQVIIMQSYCR----RKLAQ---------------------QAAKKRREAADKIRA 778
            |:.||.:|:...:.:|.|||    |:||:                     ||.::.|.||..|:|
Human   780 QKVKYHRLKGATLTLQRYCRGHLARRLAEHLRRIRAAVVLQKHYRMQRARQAYQRVRRAAVVIQA 844

  Fly   779 FIKGFITRNDAPNGFNEE---FIANAKRMW---------------------LLRLAKELPTKVLD 819
            |.:....|........|.   .|....|.|                     :|:..:||....:
Human   845 FTRAMFVRRTYRQVLMEHKATTIQKHVRGWMARRHFQRLRDAAIVIQCAFRMLKARRELKALRI- 908

  Fly   820 KSWPHAPGHCEEASGILHRLHRLHLA---RIYRL--KLTPQQKRQFELKVLAEKVFKGKKNNYAS 879
                       ||....| |.||::.   ::.:|  |:..|.|   |.|.|:|::        :.
Human   909 -----------EARSAEH-LKRLNVGMENKVVQLQRKIDEQNK---EFKTLSEQL--------SV 950

  Fly   880 SVSTWFQE-DRIPKE--HIQ---------RVNDFVASTFGSEQLKYQSFCTKFDRHGYKS---RD 929
            :.||:..| :|:.||  |.|         |:.:.|.|.....|..:.......|.|..:.   |.
Human   951 TTSTYTMEVERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSERKILEDAHSREKDELRK 1015

  Fly   930 RFILLSNKAIYVLDGKTYKQKHRLPLDKIDFTLTNHNDDLM 970
            |...|..:...:.|.|.......|...|.:|...:..::||
Human  1016 RVADLEQENALLKDEKEQLNNQILCQSKDEFAQNSVKENLM 1056

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo61F	NP_476934.2	MYSc_Myo1	52..707	CDD:276829	264/686 (38%)
		Myosin_TH1	863..1049	CDD:461801	27/123 (22%)
MYO5B	NP_001073936.1	Requires for interaction with LIMA1. /evidence=ECO:0000269|PubMed:29880681	21..40		5/11 (45%)
		MYSc_Myo5	83..749	CDD:276831	264/686 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	596..630		6/33 (18%)
		Actin-binding. /evidence=ECO:0000255	640..662		11/21 (52%)
		IQ	811..833	CDD:197470	2/21 (10%)
		IQ	860..881	CDD:197470	4/20 (20%)
		SMC_prok_B	897..>1463	CDD:274008	41/184 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1093..1123
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1166..1192
		Myo5b_CBD	1474..1845	CDD:271261