Protein Alignment Myo61F and MYH4

DIOPT Version :10

• Sequence 1: NP_476934.2
• Gene: Myo61F / 38153
• FlyBaseID: FBgn0010246
• Length: 1052
• Species: Drosophila melanogaster
• Sequence 2: NP_060003.2
• Gene: MYH4 / 4622
• HGNCID: 7574
• Length: 1939
• Species: Homo sapiens
• Alignment Length: 1153
• Identity: 336/1153 - (29%)
• Similarity: 543/1153 - (47%)
• Gaps: 187/1153 - (16%)

  Fly     4 EIKSLIFAIMDTKDYIDFNSQLKMETGLHERDRAG--------------------VQDFVLLENY 48
            :.|:.:|.:...:.|:....|.:....:..:..||                    ::|..:: .:
Human    33 DAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMM-TH 96

  Fly    49 QSEEAFIGNLKKRFQEDLIYTYIGQVLISVNPYKQLPIYTDDHVKAYRNKHFYEMPPHIFAVTDN 113
            ..|.|.:.|||:|:...:||||.|...::|||||.||:|..:.|.|||.|...|.|||||:::||
Human    97 LHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDN 161

  Fly   114 AFRSLIEENRGQCVLISGESGSGKTEASKKVLQFIA--ACSGNQTTVE--------GVKDKLLKS 168
            |::.::.:...|.:||:||||:|||..:|:|:|:.|  |.:|.:...|        .::|:::.:
Human   162 AYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISA 226

  Fly   169 NPVLEAFGNAKTNRNDNSSRFGKYMDIQFDFKGAPIGGNILNYLLEKSRVVAQMGGERNFHIFYQ 233
            ||:|||||||||.||||||||||::.|.|...|.....:|..||||||||..|:..||::|||||
Human   227 NPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQ 291

  Fly   234 LLAGADEALLQELRLERALDTYSYLTDGLNGTVTRINDADSFKQVQQALTVIDFTKEEQREIFGI 298
            :|:.....|::.|.:......:::::.| ..||..|:|.:.......|:.::.||.:|:..|:.:
Human   292 ILSNKKPELIEMLLITTNPYDFAFVSQG-EITVPSIDDQEELMATDSAVDILGFTADEKVAIYKL 355

  Fly   299 VASILHLGNVGFTEV--EGNAKVNSRDLVVTAARLLGVNASELEAALTHRTIDARGDVVTSPLNQ 361
            ..:::|.||:.|.:.  |..|:.:..::...||.|..:|:::|..:|.:..:....:.||.....
Human   356 TGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTV 420

  Fly   362 ELAIYARDALAKAVYDRLFSWLVQRLNISLQAKETRASRNNVMGILDIYGFEIFQKNSFEQFCIN 426
            :....|..|||||:|:::|.|:|.|:|   |..:|:..|...:|:|||.|||||..||.||.|||
Human   421 QQVYNAVGALAKAIYEKMFLWMVTRIN---QQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCIN 482

  Fly   427 FCNEKLQQLFIELTLKSEQDEYRREGIEWIPVEYFDNKVICNLIEEKHKGIISILDEECLRPGEP 491
            |.||||||.|.......||:||::|||||..:::..:...|..:.||..||.|||:|||:.| :.
Human   483 FTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFP-KA 546

  Fly   492 TDKTFLEKL-TQKLAQHHHYVCHEKAPAHIKKIMLRDEFRLVHYAGEVTYSVNGFLDKNND---- 551
            ||.:|..|| .|.|.:.:::  .:..||..|.   ...|.||||||.|.|::.|:||||.|
Human   547 TDTSFKNKLYEQHLGKSNNF--QKPKPAKGKP---EAHFSLVHYAGTVDYNIAGWLDKNKDPLNE 606

  Fly   552 ---------------LLFRDLKETLSKAGNGIVRNCFPEKELRSLKRPETAITQFRASLNNLMDI 601
                           .||...:...::.|.|....   :|:..|.   :|....||.:||.||..
Human   607 TVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGG---KKKGSSF---QTVSALFRENLNKLMTN 665

  Fly   602 LMCKEPSYIRCIKPNDLQTANVFNDELVLHQVKYLGLMENLRVRRAGFAYRRTYELFLERYKSLS 666
            |....|.::|||.||:.:|......||||||::..|::|.:|:.|.||..|..|..|.:|||.|:
Human   666 LRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLN 730

  Fly   667 KSTWPNYKGPGGPKAGVQQLVKDLGWDEEKYRVGETKLFIRWPRTLFDTEDAYQEKKHEIAAIIQ 731
            .|..|..:.....||. ::|:..:..|..:|:.|.||:|.: ...|...|:...||..::....|
Human   731 ASAIPEGQFIDSKKAS-EKLLGSIEIDHTQYKFGHTKVFFK-AGLLGTLEEMRDEKLAQLITRTQ 793

  Fly   732 AHWKGLMQR---RKYLKLRAQVIIMQSYCRRKLAQQAAKKRREAADKIRAFIKGFITRNDAPNGF 793
            |..:|.:.|   ||.::.|..:..:| |..|...........:...||:..:|...|..:..| .
Human   794 AICRGFLMRVEFRKMMERRESIFCIQ-YNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMAN-M 856

  Fly   794 NEEFIANAKRMWLLRLAK-ELPTKVLDKSWPHAPGHCEEASGILHRLHRLHLARIYRLKLTPQQK 857
            .|||....:     .||| |...|.|::                            ::....|:|
Human   857 KEEFEKTKE-----ELAKTEAKRKELEE----------------------------KMVTLMQEK 888

  Fly   858 RQFELKVLAE-KVFKGKKNNYASSVSTWFQEDRIPKEHIQRVND--FVASTFGSEQLKYQSFCTK 919
            ...:|:|.|| ......:......:.|..|.:...||..:|..|  .:.:...:::.|.:..|::
Human   889 NDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSE 953

  Fly   920 F----------------DRHGYKSR-----------DRFILLSNKAIYVLDGKTYKQKHRLPLDK 957
            .                ::|..:::           |..|....|     :.|..::.|:..||.
Human   954 LKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTK-----EKKALQEAHQQTLDD 1013

  Fly   958 IDF------TLTNHN-------DDL-----MVIRIPLDLKKDK----GDLILIIPRIIEFSTYII 1000
            :..      |||...       |||     ...::.:||::.|    |||     ::.:.||  :
Human  1014 LQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDL-----KLAQEST--M 1071

  Fly  1001 DTVGTASIVSIVDRNSLEHNVVKGKGGVIDIQTGAEPGVVRDKGHLVI 1048
            ||..        |:..|...:.|.:..:.::|     |.:.|:..|.|
Human  1072 DTEN--------DKQQLNEKLKKKEFEMSNLQ-----GKIEDEQALAI 1106

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo61F	NP_476934.2	MYSc_Myo1	52..707	CDD:276829	254/686 (37%)
		Myosin_TH1	863..1049	CDD:461801	43/238 (18%)
MYH4	NP_060003.2	Myosin_N	33..77	CDD:460670	6/43 (14%)
		MYSc_Myh4	100..770	CDD:276879	254/686 (37%)
		Actin-binding. /evidence=ECO:0000250	659..681		10/21 (48%)
		Actin-binding. /evidence=ECO:0000250	761..775		4/14 (29%)
		Myosin_tail_1	850..1927	CDD:460256	56/316 (18%)