Protein Alignment Klp61F and KIF14

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_055690.1
• Gene: KIF14 / 9928
• HGNCID: 19181
• Length: 1648
• Species: Homo sapiens
• Alignment Length: 1032
• Identity: 287/1032 - (27%)
• Similarity: 462/1032 - (44%)
• Gaps: 218/1032 - (21%)

  Fly     7 NTS--RQPQKKSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRS 69
            |||  :.|.|..|..:.|.|||||...||:..::::||.:.| :|:...|. |:|....|.:|.|
Human   344 NTSAGKDPLKVENSQVTVAVRVRPFTKREKIEKASQVVFMSG-KEITVEHP-DTKQVYNFIYDVS 406

  Fly    70 F------GPE-SKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWED 127
            |      .|. :.|..||..:.:||:|....|:|..:|||||||:||::||:|           .
Human   407 FWSFDECHPHYASQTTVYEKLAAPLLERAFEGFNTCLFAYGQTGSGKSYTMMG-----------F 460

  Fly   128 DSDIGIIPRALSHLFDEL---RMMEVEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGS 189
            ..:.|||||....||.::   :..||.|.:.:|:.|:|||::.|||...|....|......::..
Human   461 SEEPGIIPRFCEDLFSQVARKQTQEVSYHIEMSFFEVYNEKIHDLLVCKDENGQRKQPLRVREHP 525

  Fly   190 V---IIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVV-HIRENGIEGE- 249
            |   .::.|....|.|..|:...||.|.::|.||.|.||.:|||||:||::|: ..:...:|||
Human   526 VYGPYVEALSMNIVSSYADIQSWLELGNKQRATAATGMNDKSSRSHSVFTLVMTQTKTEFVEGEE 590

  Fly   250 -DMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVD----RAPHVPYR 309
             |.....::||:||||||..|.| :..|.|::|.|:||:||||||:||:||.:    |:..:|||
Human   591 HDHRITSRINLIDLAGSERCSTA-HTNGDRLKEGVSINKSLLTLGKVISALSEQANQRSVFIPYR 654

  Fly   310 ESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKE 374
            ||.||.||:|||||.:||::||||||...:||||||||.||::|:.|.|..:||:.:..| :::|
Human   655 ESVLTWLLKESLGGNSKTAMIATISPAASNIEETLSTLRYANQARLIVNIAKVNEDMNAK-LIRE 718

  Fly   375 YTEEIDKLKRDLMAARDKNGIYLAEETY----GEIT---LKLESQNRELNEKMLLLKALKDELQN 432
            ...||.|||     |..:|...:..|.|    .|||   :||..|.|::.|   :.:..|::.:.
Human   719 LKAEIAKLK-----AAQRNSRNIDPERYRLCRQEITSLRMKLHQQERDMAE---MQRVWKEKFEQ 775

  Fly   433 KEKIFSEVSMSLVEKTQELKKTE---------ENLLNTKGTLLLTKKVL------TKTKRRYKEK 482
            .||       ..:::|:||:|..         .||:|......|::.:|      |.|..:||..
Human   776 AEK-------RKLQETKELQKAGIMFQMDNHLPNLVNLNEDPQLSEMLLYMIKEGTTTVGKYKPN 833

  Fly   483 KELVASHMKTEQVLTTQAQEILAAADLAT--------------------DDTHQLHGTIERRREL 527
                :||       ..|...:|.|.|..|                    :..|.|..|:.|.
Human   834 ----SSH-------DIQLSGVLIADDHCTIKNFGGTVSIIPVGEAKTYVNGKHILEITVLRH--- 884

  Fly   528 DEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQEMVNSSYVSQRLALNSS------ 586
            .:::....|.: .|....:|:..|.....:|...:...:..:...|...::||..|.:.
Human   885 GDRVILGGDHY-FRFNHPVEVQKGKRPSGRDTPISEGPKDFEFAKNELLMAQRSQLEAEIKEAQL 948

  Fly   587 -------KSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQQQLL----- 639
                   :.|::.|||..|.|..|...:...|           :|..|:|.|:.|::::.
Human   949 KAKEEMMQGIQIAKEMAQQELSSQKAAYESKI-----------KALEAELREESQRKKMQEINNQ 1002

  Fly   640 ----MSKEIQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSLP 700
                ..:|::...|.:|:....:|..|:  .|..||     .|::|:|:.:..:.||.|......
Human  1003 KANHKIEELEKAKQHLEQEIYVNKKRLE--METLAT-----KQALEDHSIRHARILEALETEKQK 1060

  Fly   701 DAEELQNLQEELAN-ERALAQQE--DALLESMMMQMEQIKNLRSKNSISMSVHLNKMEESRLTRN 762
            .|:|:|.||:...| ::....|.  .::..|||:|.....:.:.|.......| :..::|....:
Human  1061 IAKEVQILQQNRNNRDKTFTVQTTWSSMKLSMMIQEANAISSKLKTYYVFGRH-DISDKSSSDTS 1124

  Fly   763 HRIDDIKSGIQDYQKL-GIEASQSAQAEL-------------------------------TSQME 795
            .|:.::|.||..:..| ..|:..:|..||                               .|:..
Human  1125 IRVRNLKLGISTFWSLEKFESKLAAMKELYESNGSNRGEDAFCDPEDEWEPDITDAPVSSLSRRR 1189

  Fly   796 AGMLCLDQGVANC-SMLQVH-MKNLNQKYEKETNENVGSVRVHHNQVEI----ICQESKQQLEAV 854
            :..|..::.::.| ..:||| :|||:..:.....:.  |..::.|..|.    ||:|.
Human  1190 SRSLMKNRRISGCLHDIQVHPIKNLHSSHSSGLMDK--SSTIYSNSAESFLPGICKEL------- 1245

  Fly   855 QEKTEVNLEQMVDARQQLITEDRQRFIGHATVATDLVQE----SNRQFSEHAEHQRQ 907
                   :...:|...|...|:|       |:|..|:..    .|..|:....|:.|
Human  1246 -------IGSSLDFFGQSYDEER-------TIADSLINSFLKIYNGLFAISKAHEEQ 1288

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	151/367 (41%)
		SMC_prok_B	<408..728	CDD:274008	75/379 (20%)
		SMC_prok_B	<651..>921	CDD:274008	61/302 (20%)
		Microtub_bind	923..>1004	CDD:464048
KIF14	NP_055690.1	Required for PRC1-binding	1..356		5/11 (45%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..27
		Required for microtubule-binding with high affinity. /evidence=ECO:0000250|UniProtKB:L0N7N1	356..737		160/400 (40%)
		KISc_KIF1A_KIF1B	357..708	CDD:276816	148/364 (41%)
		Kinesin_assoc	705..823	CDD:465047	33/133 (25%)
		FHA_KIF14	794..901	CDD:438759	22/121 (18%)
		Required for CIT-binding	901..1648		83/430 (19%)
		Smc	<927..>1077	CDD:440809	37/167 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1600..1648