Protein Alignment Klp61F and KIF20B

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_001271188.1
• Gene: KIF20B / 9585
• HGNCID: 7212
• Length: 1820
• Species: Homo sapiens
• Alignment Length: 1228
• Identity: 272/1228 - (22%)
• Similarity: 493/1228 - (40%)
• Gaps: 343/1228 - (27%)

  Fly    15 KSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRH------TLDSK----LTKKFTFDRS 69
            :|...:||.:|:||....|:.:.|...|.::..:.||.:.      .|..|    :.:||:|.:.
Human    54 ESKDYLQVCLRIRPFTQSEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKV 118

  Fly    70 FGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGII 134
            |||.:.|.:.:...:...::::|.|.:..:|.||.|.:|||:|..|.|           .:|||:
Human   119 FGPATTQKEFFQGCIMQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTE-----------ENIGIL 172

  Fly   135 PRALSHLFDEL-------------RMME------------------------------------- 149
            ||.|:.|||.|             |..|
Human   173 PRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSSEQEKEEIASKSALLRQIKEVTVHNDSDDTLY 237

  Fly   150 -----------------------------VEYTMRISYLELYNEELCDLL-----STDDTTKIRI 180
                                         :::::.:|:.|:|||.:.||.     .......:|:
Human   238 GSLTNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYNEYIYDLFVPVSSKFQKRKMLRL 302

  Fly   181 FDDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSI-VVHIREN 244
            ..|  .||...|:.|:.|.|....:.|:||:.|.:.:..|.|.:|..|||||::|:: ::.|.::
Human   303 SQD--VKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSRSHSIFTVKILQIEDS 365

  Fly   245 GIEGEDMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVDR-----AP 304
              |...::::.:|:|.||||||...|..|| |.|:|||.|||.||||||:.|..|.:.     ..
Human   366 --EMSRVIRVSELSLCDLAGSERTMKTQNE-GERLRETGNINTSLLTLGKCINVLKNSEKSKFQQ 427

  Fly   305 HVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVN---QKL 366
            |||:||||||...|....|:.|..:|..||..:...:|||:.|:::..|:.:.....:|   :||
Human   428 HVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVCVPDTLNSSQEKL 492

  Fly   367 -----------------TKKTVLKEYTEEIDKLKRDLMAARD-----KNGIYLAEETYG-EITLK 408
                             :|...:|..|...:....|||...|     :|    ||||.. |..|.
Human   493 FGPVKSSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELEN----AEETQNVETKLL 553

  Fly   409 LESQNRELNE-----------KML-LLKALKDELQN--KEKIFSEVSMSLVEKTQELKK-TEENL 458
            .|..::.|.|           |:| |::.||.:|.|  |||:..|..:. .|.|||..: ..:..
Human   554 DEDLDKTLEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFKIR-EEVTQEFTQYWAQRE 617

  Fly   459 LNTKGTLLLTKKVLTK-TKRRYKEKKELVASHMKTEQVLTTQAQEILAAADLATDDTHQLHGTIE 522
            .:.|.|||..:::|.: .:||....|:||......|     :|.:.:.|..:.|::||...|
Human   618 ADFKETLLQEREILEENAERRLAIFKDLVGKCDTRE-----EAAKDICATKVETEETHNYVG--- 674

  Fly   523 RRRELDEKIRRSCDQFKDRMQDNLEMI---GGSLNLY-------QDQQAALKEQLSQEMVNSSYV 577
                        .:...|.:|||:..|   ....:||       |:..|.|:.:.:|       :
Human   675 ------------FEDIIDSLQDNVADIKKQAEIAHLYIASLPDPQEATACLELKFNQ-------I 720

  Fly   578 SQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQQQLLMSK 642
            ...||                          |..||::|..::         :::.:.:...:.:
Human   721 KAELA--------------------------KTKGELIKTKEE---------LKKRENESDSLIQ 750

  Fly   643 EIQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSL-------- 699
            |::|:.:.|...|||.|.:::.:.:|..||  :..|:::.|.:...|..::....||
Human   751 ELETSNKKIITQNQRIKELINIIDQKEDTI--NEFQNLKSHMENTFKCNDKADTSSLIINNKLIC 813

  Fly   700 -------PDAEELQNLQEELANERALAQQE-----------DALLESMMMQME------QIKNLR 740
                   .|::.....:.:..||..|.|.|           .|:.|......|      :|:::|
Human   814 NETVEVPKDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIR 878

  Fly   741 --SKNSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQ 803
              .:|:..:...|..:|...........::...|..:|:   |.|.|.:..||...|...:   |
Human   879 VLQENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQ---ELSLSEKKNLTLSKEVQQI---Q 937

  Fly   804 GVANCSMLQVHM-KNLNQKYE----KETNE----------NVGSVRVHHNQVEIICQESKQQLEA 853
            ...:.::.::|: |:.||:.|    |.:||          ||..:::.|.:::.:     :.|::
Human   938 SNYDIAIAELHVQKSKNQEQEEKIMKLSNEIETATRSITNNVSQIKLMHTKIDEL-----RTLDS 997

  Fly   854 VQEKTEVNL------------EQMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHA---- 902
            |.:.:.::|            :.:.:.:..|:..|   ::....|....:||.||:.|.|:
Human   998 VSQISNIDLLNLRDLSNGSEEDNLPNTQLDLLGND---YLVSKQVKEYRIQEPNRENSFHSSIEA 1059

  Fly   903 ---------------EHQRQQLQICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQ 952
                           .||.::|   ||::.:. |:|:|.|........:::......|:  ...:
Human  1060 IWEECKEIVKASSKKSHQIEEL---EQQIEKL-QAEVKGYKDENNRLKEKEHKNQDDLL--KEKE 1118

  Fly   953 EIVRRYRQELDWSDLDTTATIDECSEGEHDVSMHS------VQELSETETIMNSTPIE 1004
            .::::.::||...::.....|....||:..:|..:      ..::.|.|||:.:..:|
Human  1119 TLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVE 1176

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	121/450 (27%)
		SMC_prok_B	<408..728	CDD:274008	74/371 (20%)
		SMC_prok_B	<651..>921	CDD:274008	64/349 (18%)
		Microtub_bind	923..>1004	CDD:464048	14/86 (16%)
KIF20B	NP_001271188.1	KISc_KIF23_like	57..477	CDD:276819	119/435 (27%)
		RBD_KIF20B	600..654	CDD:409644	15/59 (25%)
		Mplasa_alph_rch	740..1350	CDD:275316	82/459 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	829..866		7/36 (19%)
		Necessary and sufficient for interaction with SHTN1. /evidence=ECO:0000250|UniProtKB:Q80WE4	1050..1107		11/60 (18%)
		SMC_prok_B	<1077..1381	CDD:274008	20/106 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1247..1275
		Smc	<1280..>1583	CDD:440809
		PTZ00108	<1501..1792	CDD:240271
		Interaction with PIN1. /evidence=ECO:0000269|PubMed:11470801	1560..1820
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1760..1781