Protein Alignment Klp61F and Kif4a

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_001387859.1
• Gene: Kif4a / 84393
• RGDID: 620526
• Length: 1231
• Species: Rattus norvegicus
• Alignment Length: 1230
• Identity: 333/1230 - (27%)
• Similarity: 534/1230 - (43%)
• Gaps: 298/1230 - (24%)

  Fly    20 IQVYVRVRPLNSRE------RCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCD 78
            ::|.:|.|||.|:|      .|:..     |.|..:||.      ...|.||:|..|.|.::|.:
  Rat    10 VRVALRCRPLVSKEINEGCQTCLSF-----VPGEPQVVV------GTDKSFTYDFVFDPSTEQEE 63

  Fly    79 VYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFD 143
            |::..|:|||:.|..|||.||.||||||:|||::|.|..|||    .|.||.||:|||.:..||.
  Rat    64 VFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAE----QEHDSAIGVIPRVIQLLFK 124

  Fly   144 EL-RMMEVEYTMRISYLELYNEELCDLL--STDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDD 205
            |: :..:.|:|:::||||:||||:.|||  |.:..::|.|.:| .|:|..|: ||.|..|....|
  Rat   125 EINKKTDFEFTLKVSYLEIYNEEILDLLCSSREKASQINIRED-PKEGIKIV-GLTEKTVLVASD 187

  Fly   206 VYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSK 270
            ....||:|...|..|:|.||:||||||.:|:|.:..|:...:.....  .||:||||||||...|
  Rat   188 TVSCLEQGNNCRTVASTAMNSQSSRSHAIFTISIEQRKKNDKNSSFR--SKLHLVDLAGSERQKK 250

  Fly   271 AGNEKGIRVRETVNINQSLLTLGRVITALVD--RAPHVPYRESKLTRLLQESLGGRTKTSIIATI 333
            ...| |.|::|.:|||:.||.||.||:||.|  :...||||:||||||||:||||.:.|.:||.:
  Rat   251 TKAE-GDRLKEGININRGLLCLGNVISALGDDKKGNFVPYRDSKLTRLLQDSLGGNSHTLMIACV 314

  Fly   334 SPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLA 398
            ||...::||||:||.||.||:.|:|||.:|        :.....|::.||:
  Rat   315 SPADSNLEETLNTLRYADRARKIKNKPIIN--------IDPQAAELNHLKQ-------------- 357

  Fly   399 EETYGEITLKLESQNRELNEKMLLLKALKDELQNKEKIF-SEVSMSLVEKTQELKKTEENLLNTK 462
                         |.::|  ::|||:|....|.....:. ||...||:||.|.|  .|||...::
  Rat   358 -------------QVQQL--QVLLLQAHGGTLPGNINVKPSENLQSLMEKNQSL--VEENEKLSR 405

  Fly   463 G-------------TLLLTKKVLTKTKRRYKEKKELVASHMKTEQVL-TTQAQEI---------- 503
            |             .::||::...|...:.:|.::..|..:..:::. |.:.||:
  Rat   406 GLSEAAGQTAQMLERIILTEQANEKMNAKLEELRQHAACKVDLQKLAETLEDQEVKENIEIICNL 470

  Fly   504 -LAAADLATDDTHQLHGTIERRRELDEK------IRRSCDQFKD-----RMQDNLEMIGGSLNLY 556
             .|.|.|:.:....:..||:...|:|.:      ..||.|.|..     :.|.:.|:|  .||
  Rat   471 QQAIARLSDEAVACMTATIDTAGEVDTQEQSSPDTSRSSDVFSTQHALRQAQMSKELI--ELN-- 531

  Fly   557 QDQQAALKEQLSQEMVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVM------ 615
              ::.||||.|:::|..:....|.:......:|:.| |:...|||.:   ..:|:.|:.
  Rat   532 --KELALKEALAKKMTQNDNQLQPIQFQYQDNIKNL-ELEVLSLQKE---KEELVLELQTAKKDV 590

  Fly   616 ---KISD------QHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEEN-------NQRHKAMLDS 664
               |:|:      |..:..:|:|.:::.:|..|:..:..|.|.|.:.|       |||.:.|...
  Rat   591 NQAKLSERRRKRLQELEGQIAELKKKLHEQSKLLKLKETTELTVSKLNQEIRMMKNQRVQLMRQM 655

  Fly   665 ----------MQEKFATII-----DSSLQ--------SVEEHAKQMHKKLEQLGAMSLPDAEELQ 706
                      .|:|...:|     |...|        :.::.:..:.:|.|:..|.:....:.||
  Rat   656 KEDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQ 720

  Fly   707 NLQEELANERALAQQED-----ALLES-------MMMQMEQIKNLRSKNSISMSVHLNKMEESRL 759
            . |:|:|.:|...|...     |.::|       :|:..|:.|.           |||.:.|.|.
  Rat   721 K-QKEVAEKRKETQSRGMEGTAARMKSWLGNEIEVMVSTEEAKR-----------HLNDLLEERK 773

  Fly   760 TRNHRIDDIK----SGIQDYQKL---------------GIEASQSAQAE-LTSQMEAGMLCLDQG 804
            .....:..:|    ||.....||               |.|.|.|.|.| |.|::|
  Rat   774 ILAQDVAQLKEKRESGENPPPKLRRRTFSRDEVHGQDSGAEDSISKQIESLESELE--------- 829

  Fly   805 VANCSMLQVHMKNLNQKYE---KETNENVGSVRVHHNQVEIICQE---SKQQLEAVQEKTEVNLE 863
            :.:..:..:..|.|:.:.|   |:..||:.::......::.:..|   ||.|:..::.....:..
  Rat   830 LRSAQIADLQQKLLDAESEDQPKQRWENIATILEAKCAIKYLVGELVSSKIQVSKLESGLSQSKA 894

  Fly   864 QMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAE------HQRQQLQICEQEL------ 916
            ..::. |:::.|::..|   |.:.|:|.:|..|...:|.|      .|.||.|:.|::|
  Rat   895 SCIEV-QKMLFEEQNHF---AKIETELKEELVRVEQQHQEKVLYLLSQLQQCQVTEKQLEESVSE 955

  Fly   917 --------VRFQQSELKTYAPT---------GTTPSKRDFVYPRTLVATSPHQEIVRRYRQELDW 964
                    ::.|:.||:.....         ..:..|:.....:.:....||  :||...|..|.
  Rat   956 KEQQLLSTLKCQEEELRKMQEVCEQNQQLLQENSAIKQKLNLLQVVSKEKPH--LVRNILQSPDS 1018

  Fly   965 S-------------------------DLDTTATIDECSEGEHDVSMHSVQELSETETIMNSTPIE 1004
            |                         ||...:......|...|...|:.:|...|:.:..|.  :
  Rat  1019 SFEYIPPKPKPFRIKEKCPEKSFAIEDLQYYSETSVAEEENEDSDDHADEEWIPTKLVKVSK--K 1081

  Fly  1005 PVDGVTVKRGCGTTRNSNSNALKPPVATGGKRSSSLSRSLTPSKTSPRGSPAFVRHNKEN 1064
            .:.|.:.|..||   |......|.      |...:.:.|..|:|...|      .|:::|
  Rat  1082 NIQGCSCKGWCG---NKQCGCRKQ------KSDCNGACSCDPTKCRNR------HHSQDN 1126

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	160/355 (45%)
		SMC_prok_B	<408..728	CDD:274008	89/406 (22%)
		SMC_prok_B	<651..>921	CDD:274008	70/357 (20%)
		Microtub_bind	923..>1004	CDD:464048	18/114 (16%)
Kif4a	NP_001387859.1	KISc_KIF4	8..338	CDD:276823	155/347 (45%)
		PTZ00121	<397..1039	CDD:173412	134/678 (20%)
		SMC_prok_B	550..>865	CDD:274008	69/339 (20%)
		SET	1084..>1121	CDD:394802	11/51 (22%)