Protein Alignment Klp61F and kif21a

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_009296478.1
• Gene: kif21a / 566481
• ZFINID: ZDB-GENE-110411-237
• Length: 1598
• Species: Danio rerio
• Alignment Length: 1319
• Identity: 324/1319 - (24%)
• Similarity: 524/1319 - (39%)
• Gaps: 363/1319 - (27%)

  Fly    17 NQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYS 81
            :..::|.:|:||..::|: |....:...|.|.|.......|    |.||:|..|..:|.|.::||
Zfish     3 DSTVRVALRIRPQLAKEK-IEGCHICTFVMPDEPQVVLGKD----KAFTYDYVFDMDSTQDNIYS 62

  Fly    82 VVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTM-VGNETAELKSSWEDDSDIGIIPRALSHLFD-- 143
            .....|||....|||.|:|||||||:|||:|| .|.:.|      ..|.|:||||||::|||.
Zfish    63 NCTEKLIEGCFEGYNATIFAYGQTGSGKTYTMGTGFDVA------IPDEDLGIIPRAVTHLFKGI 121

  Fly   144 ELRMMEV--------EYTMRISYLELYNEELCDLLSTDDTTK----------IRIFDDSTKKGSV 190
            |.|..:.        |:.:...:|||||||:.||.   |||:          |:|.:|::  |.:
Zfish   122 EQRRQQAAEQSRPVPEFKISAQFLELYNEEVLDLF---DTTRDMESRKQKSHIKIHEDAS--GGI 181

  Fly   191 IIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSI-VVHIR------------ 242
            ...|:....|.|:.::.:.|:.|...|.||:|.||.||||||.:|:| :..||
Zfish   182 YTVGVTTRNVSSEAEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHICQIRVCAPADSPPEQD 246

  Fly   243 ---ENGIEG------EDMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITA 298
               :|.:.|      |......|.:.|||||||.:.:.| ..|.|.:|.::||..||.||.||:|
Zfish   247 NETDNRLAGSSSEMEEFETLTAKFHFVDLAGSERLKRTG-ATGERAKEGISINCGLLALGNVISA 310

  Fly   299 LVD---RAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKP 360
            |.|   |:.|||||:||||||||:||||.::|.:||.|||..:|..|||:||:||:||:||:||.
Zfish   311 LGDRSKRSTHVPYRDSKLTRLLQDSLGGNSRTVMIACISPSDQDFMETLNTLKYANRARNIKNKV 375

  Fly   361 EVNQKLTKKTVLKEYTEEIDKLKRDLMAAR------DKNGIYLAEETYGEIT-LKLESQNRELNE 418
            .|||....:.:....| ||.:|:.:||..|      .::|:....:.:.|.: |::|:.|..:..
Zfish   376 MVNQDRASQQISALRT-EIARLQMELMEYRTGKRMVGEDGLESINDMFHENSMLQVENNNLRMRV 439

  Fly   419 K----------------------MLLLKA------LKDELQNKEKIFSEVSMSLVEKTQELKKTE 455
            |                      .||.:|      :.:.:||..|...::...|:|.....:...
Zfish   440 KAMQEAIDAQGARLTQLLSQQANQLLARAGEGSEEIGNMIQNYIKEIEDLRAKLLESEAVNENLR 504

  Fly   456 ENLL--------------------------NTKGTLLLTKKVLTKTKRRYKEKK----ELVA--- 487
            .||.                          .|...:.:.||.|.|.|||.|:||    :|:|
Zfish   505 RNLTRASSRPQFYGASGSFSTALLGPDPSQETSDIIEIAKKDLEKLKRREKKKKKRLQQLLADKR 569

  Fly   488 -----------------------SHMKT--EQVLTTQAQEILAAADLATDDTHQLHGTIERRR-- 525
                                   ...||  ||..:|..:..:.|:|....|..:....:|...
Zfish   570 DEDEDEEEEVEEDSANKDETQEDGEQKTEKEQSESTNQEPEMEASDREEGDGEEDEEDVEEEEME 634

  Fly   526 ----------ELDEK-------IRRSCD-QFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQEMV 572
                      |||||       ...:|: ..|.::.|.||.....|:..: ||...|..:.|..:
Zfish   635 AEESSEETDSELDEKEDFQADLANITCEIAIKQKLIDELENSQRRLHTLK-QQYEHKLMMLQSKI 698

  Fly   573 NSSYVSQRLALNSSKSIE--------------------MLKEMCAQSLQDQTNLHNKLIGEVMKI 617
            ..:.:.:...|:|..|:|                    |.||:  |.||.....|.:|:....:.
Zfish   699 RDTQLERDRVLHSMGSVESCSEDKTKRIKAEYEKKLSVMNKEL--QKLQAAQKEHARLLKNQSQY 761

  Fly   618 SDQ---------HSQAFVAKLMEQMQQQQ-------LLMSKEIQTNLQVIEENNQRHKAMLDSMQ 666
            ..|         ..:....:||:||::||       ...::||.|..:  ::..|.|:..|...|
Zfish   762 EKQLKKLQLDLTEMKKTKVRLMKQMKEQQEKSRMAESRRNREIATLKK--DQRKQEHQLKLLEAQ 824

  Fly   667 EKFATII----DSSLQSVEEHAKQMHKKLEQLGAMSLPDAE------------------------ 703
            ::...:|    ...:.::....:.:..|:.:...:|.|:.:
Zfish   825 KRQQELILRRKTEEVTALRRQVRPISGKVTRKANLSEPNFDLSHRPPAGRTYGSGAPNGTRLYHR 889

  Fly   704 -------------ELQNLQEELAN---ERALAQQEDALLESMMMQMEQIKNLRSK---------- 742
                         :.|:|:..:::   :|......:|.:..::.|.|::...|.|
Zfish   890 RAAGIYSTRVARGKWQSLERRISDIIMQRMTISNMEADMNRLLKQREELTRRRDKISRKRERLLA 954

  Fly   743 NSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGVAN 807
            :...:..||..:.|...:....||.|...|.|.|...::..::.:       |...:.:...:::
Zfish   955 DDPEVEKHLQPVNEELESLLANIDYINDSISDCQANIMQMEEAKE-------EGDAVDVSAVISS 1012

  Fly   808 CSMLQV-----HMKN--LN---QKYEKETNENVGSVRVHHNQVEIICQES------KQQLE---- 852
            |::.:.     |..:  ||   |..:||:...|...|:...::....|..      |::.|
Zfish  1013 CTLAEARFLLDHFMSMALNKGLQAAQKESQIKVMEGRLKQTEINSATQNQLLFHMLKEKAEFNPE 1077

  Fly   853 -------AVQEKTEVNLEQMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQ 910
                   |:||...|.||...|:.....|....  :...|:|:||::......|.....:|...|
Zfish  1078 LDALLGNALQELGNVPLENGDDSSSDESTPSPA--VEGNTLASDLMKLCGETKSRTKARRRTTTQ 1140

  Fly   911 ICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIVRRYRQELDWSDLDTTATIDE 975
            :   ||:         |..:|...:.:||..|...:|                           |
Zfish  1141 M---ELL---------YVDSGQDAATKDFTIPLHPMA---------------------------E 1166

  Fly   976 CSEGEHDVSMHSVQELSETETIMNSTPIEPVDGVTVKRGCGTTRNSNSNALKPPVATGGKR---S 1037
            .|||..::.       |...|:.:...:....|:|.:.| |.:.:.|    :.| |.|.||   .
Zfish  1167 TSEGSGELE-------SAGNTVRDRDYMPSPAGLTSRMG-GISSSGN----RFP-AGGQKRLPEP 1218

  Fly  1038 SSLSRSLTPSKTSPRGSPA 1056
            |.|||..|..|...:...|
Zfish  1219 SPLSRRKTYDKGQAQADKA 1237

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	157/393 (40%)
		SMC_prok_B	<408..728	CDD:274008	84/505 (17%)
		SMC_prok_B	<651..>921	CDD:274008	55/350 (16%)
		Microtub_bind	923..>1004	CDD:464048	12/80 (15%)
kif21a	XP_009296478.1	KISc_KIF4	4..372	CDD:276823	152/384 (40%)
		GumC	304..>513	CDD:442439	71/209 (34%)
		EnvC	647..>856	CDD:443969	41/213 (19%)
		Laminin_I	905..>1091	CDD:310534	33/192 (17%)
		Rcc_KIF21A	917..998	CDD:410204	15/80 (19%)
		WD40	1266..1577	CDD:238121
		WD40 repeat	1278..1312	CDD:293791
		WD40 repeat	1317..1351	CDD:293791
		WD40 repeat	1371..1414	CDD:293791
		WD40 repeat	1420..1464	CDD:293791
		WD40 repeat	1471..1505	CDD:293791
		WD40 repeat	1512..1536	CDD:293791