Protein Alignment Klp61F and kif5aa

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_001186705.1
• Gene: kif5aa / 566086
• ZFINID: ZDB-GENE-070912-141
• Length: 1033
• Species: Danio rerio
• Alignment Length: 1121
• Identity: 299/1121 - (26%)
• Similarity: 494/1121 - (44%)
• Gaps: 239/1121 - (21%)

  Fly    19 NIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLT--KKFTFDRSFGPESKQCDVYS 81
            ||:|..|.||||       .||::.  |.:.:.|....|:.:.  |.:.|||.|...:.|..||:
Zfish     9 NIKVLCRFRPLN-------QAEILR--GDKFLPTFQGDDTVIIGGKSYAFDRVFPTNTTQEQVYN 64

  Fly    82 VVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELR 146
            .....::::||.|||.|:||||||.:||||||.||        ..|...:|||||....:|:.:.
Zfish    65 TCAKQIVKDVLGGYNGTIFAYGQTSSGKTHTMEGN--------LHDPQQMGIIPRIAEDIFNHIF 121

  Fly   147 MME--VEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKL 209
            .|:  :|:.:::||.|:|.|::.|||   |.||..:.....|.....::|..|..|.|.::|..:
Zfish   122 SMDENLEFHIKVSYFEIYMEKIRDLL---DVTKTNLSVHEDKNRVPYVKGCTERFVSSPEEVMDV 183

  Fly   210 LEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKAGNE 274
            :::||..|..|.|.||..|||||::|  :::|::..:|.|..| .|||.||||||||.|||.| .
Zfish   184 IDEGKSNRHVAVTNMNEHSSRSHSIF--LINIKQEHVETEQKL-CGKLYLVDLAGSEKVSKTG-A 244

  Fly   275 KGIRVRETVNINQSLLTLGRVITALVD-RAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHK 338
            .|..:.|..|||:||..||.||:||.: ...|||||:||:||:||:||||..:|::....||...
Zfish   245 AGAVLDEAKNINKSLSALGNVISALAEGTKSHVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSY 309

  Fly   339 DIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLK--RDLM------AARDKNG- 394
            :..||.|||.:..|||.|:|...:|.:||.:...::|.:|.:|.|  ||.|      ..|.:||
Zfish   310 NDAETKSTLMFGQRAKTIKNTASINLELTAEQWKRKYEKEKEKNKSLRDSMQRLELELRRWRNGE 374

  Fly   395 --------------IYLAEETYGEITLKLESQ---NRELNEKMLLLKALKDELQNKEKIFSEVSM 442
                          :.::|||..:.....|.|   .|:.:...::::..::|.|..|:...::..
Zfish   375 KVPETEQTDADVVKLDVSEETSLDNEKSCERQRERERDSDTSSIVVRISEEERQKYEEEIRKLYR 439

  Fly   443 SLVEKTQEL-------KKTEENLLNTKGTLLLTK----KVLTKTKRRYKEKKELVASHMKTEQVL 496
            .|.:|..|:       :|.:|.:|:....|..|:    ||.|:..|...|.:...|...:..|.|
Zfish   440 QLDDKDDEINLQCQLVEKLKEQILDQDELLASTRGDSDKVQTELGRLQTESESAKAEVREVLQAL 504

  Fly   497 -------TTQAQEI----LAAADLATDDTHQLHGTIERRREL-------DEKIRRSCDQFKDRMQ 543
                   ..::||:    |....||...:|::...:|...||       .::.:|..|.....|:
Zfish   505 EELAVNYDQKSQEVEEKSLHNKQLAEQLSHKMANLMELEAELAQMQEVSSQQRKRIADVLNGLMK 569

  Fly   544 DNLE---MIG-GSLNLYQDQQAALKEQLSQEMVNSSYVSQRLALNSSKS-IEMLKEMCAQSLQDQ 603
            |..|   ::| |.|.|..:...|::|:         :...||.::..|| ::.:.:.|.|....|
Zfish   570 DLSEFSSIVGNGELKLPVEISGAIEEE---------FTVARLYISKIKSEVKSMVKRCRQLENMQ 625

  Fly   604 TNLHNKL------IGEVMKISDQHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEE------NNQ 656
            ...|.|:      :.....:..|| :|.:..|.|.||..: ...::::.|...:.|      |::
Zfish   626 LECHRKMEETSRELSSCQLLISQH-EAKIRSLTEYMQNVE-QKKRQLEDNYDALSEELYTLQNSE 688

  Fly   657 RHKAMLDSMQEKFATIIDSSLQSVEE--HAKQMHKKLEQL-----GAMSLPD--AEELQNLQEEL 712
            .|.|.||. .||.....|....:|..  ||:....:|.:|     ....|.|  .::.|.|:.||
Zfish   689 SHVAELDG-GEKADREADGKKAAVRPALHAESRQTQLSRLRDEINEKQRLIDELTDKNQALELEL 752

  Fly   713 ANERA----LAQQED---ALLESMMMQMEQIKNLRSKNSISMSVHLNKMEES-----RLTRNHR- 764
            |:.|:    |..|:|   |.||.|..|.|  ::.:||..      |..:||:     :...|.|
Zfish   753 AHVRSSFSNLKLQDDSKSACLEQMSFQQE--RHEQSKQD------LKGLEETVARELQTLHNLRK 809

  Fly   765 --IDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGVAN-----CSMLQVHMKNLNQKY 822
              :.|:.:.::...::|.:                    |.|.:|     .|.|:.::..|.:.:
Zfish   810 LFVQDLTTRVKKSSEIGPD--------------------DSGGSNTQKQKISFLENNLDQLTKVH 854

  Fly   823 EKETNENVGSVRVHHNQVEIICQESK--QQLEAVQEKTEVNLEQMVDARQQLITEDRQRFIGHAT 885
            ::...:|          .::.|:..|  ::|.:..|:.......:.||:|..: .||:|:
Zfish   855 KQLVRDN----------ADLRCELPKLEKRLRSTAERVRALETALKDAKQGAM-NDRRRY----- 903

  Fly   886 VATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTY---APTGTTPSKRDFVYP-RTLV 946
                                       :||:.|.:.:....|   .|.....:|.  |.| ..:.
Zfish   904 ---------------------------QQEVERIRDAMRLRYPLRRPNAAQIAKP--VRPGHHVT 939

  Fly   947 ATSPHQ-EIVRRYRQELDWSDL----DTTATIDECSEGEHDVSMHSVQELSETETIMNSTPIEPV 1006
            ||||.. .:.|..:....:|:.    |...   |.|:..:.|...:|:.:.    |::|.|::
Zfish   940 ATSPTSFSVTRASKPATSYSNARFLQDEKM---ENSQKTNTVGYSTVRSID----ILSSCPLD-- 995

  Fly  1007 DGVTVKRGCGTTRNSN 1022
                |:.|..|..|.|
Zfish   996 ----VENGNSTDINDN 1007

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	141/350 (40%)
		SMC_prok_B	<408..728	CDD:274008	86/384 (22%)
		SMC_prok_B	<651..>921	CDD:274008	60/306 (20%)
		Microtub_bind	923..>1004	CDD:464048	19/89 (21%)
kif5aa	NP_001186705.1	KISc_KHC_KIF5	7..327	CDD:276820	138/341 (40%)
		SMC_prok_B	<334..614	CDD:274008	62/288 (22%)
		SMC_N	478..>809	CDD:481474	82/350 (23%)
		Khc_CBD_cc	842..911	CDD:467880	16/111 (14%)