Protein Alignment Klp61F and KIF16B

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_005260807.1
• Gene: KIF16B / 55614
• HGNCID: 15869
• Length: 1883
• Species: Homo sapiens
• Alignment Length: 1332
• Identity: 326/1332 - (24%)
• Similarity: 528/1332 - (39%)
• Gaps: 365/1332 - (27%)

  Fly    19 NIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTR------HTLDS--KLTKKFTFDRSF-GPES 74
            :::|.|||||:|.||:.:.:..::.:...:..:|.      .|.||  :.||.||:|.|| ..::
Human     3 SVKVAVRVRPMNRREKDLEAKFIIQMEKSKTTITNLKIPEGGTGDSGRERTKTFTYDFSFYSADT 67

  Fly    75 KQCD------VYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGI 133
            |..|      |:..:.:.:::....|||..||||||||:||::||:||           ..|.|:
Human    68 KSPDYVSQEMVFKTLGTDVVKSAFEGYNACVFAYGQTGSGKSYTMMGN-----------SGDSGL 121

  Fly   134 IPRALSHLFDEL----RMMEVEYTMRISYLELYNEELCDLL--STDDTTKIRIFDDSTKKGSVII 192
            |||....||..:    |..|..:...:||||:|||.:.|||  .:..|..:|: .:..|:|. .:
Human   122 IPRICEGLFSRINETTRWDEASFRTEVSYLEIYNERVRDLLRRKSSKTFNLRV-REHPKEGP-YV 184

  Fly   193 QGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLK-IGK 256
            :.|.:..|.:..||.:|::.|...|.||.|.||..|||||.:|:|  ...:...:.|...: :.|
Human   185 EDLSKHLVQNYGDVEELMDAGNINRTTAATGMNDVSSRSHAIFTI--KFTQAKFDSEMPCETVSK 247

  Fly   257 LNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVD------------RAPHVPYR 309
            ::||||||||.....| ..|:|::|..|||:||:|||.||:||.|            :...||||
Human   248 IHLVDLAGSERADATG-ATGVRLKEGGNINKSLVTLGNVISALADLSQDAANTLAKKKQVFVPYR 311

  Fly   310 ESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKE 374
            :|.||.||::||||.:||.:||||||...:..||||||.||:|||||.|||.:|:....| :::|
Human   312 DSVLTWLLKDSLGGNSKTIMIATISPADVNYGETLSTLRYANRAKNIINKPTINEDANVK-LIRE 375

  Fly   375 YTEEIDKLKRDL-----MAARDKNGIYLAEETYGEITLKLESQNRE-----------LNEKMLLL 423
            ...||.:||..|     :|..|.......||...:...:::...:|           |.|:.|.|
Human   376 LRAEIARLKTLLAQGNQIALLDSPTALSMEEKLQQNEARVQELTKEWTNKWNETQNILKEQTLAL 440

  Fly   424 KA------------------------------LKD---------------------ELQNKEKIF 437
            :.                              ||:                     :|:::..||
Human   441 RKEGIGVVLDSELPHLIGIDDDLLSTGIILYHLKEGQTYVGRDDASTEQDIVLHGLDLESEHCIF 505

  Fly   438 SEV-----------SMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTK-------TKRRYKEKKE 484
            ..:           |...|...|.::.|.   ||....:||.:..:.:       .|.|.|.|..
Human   506 ENIGGTVTLIPLSGSQCSVNGVQIVEATH---LNQGAVILLGRTNMFRFNHPKEAAKLREKRKSG 567

  Fly   485 LVASHMKTEQVLTTQAQEILAAADLATDDTHQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMI 549
            |::|...:...| ::::|.|:|..|.......:.|.|..|.|           |:.:.::.||.:
Human   568 LLSSFSLSMTDL-SKSRENLSAVMLYNPGLFPIKGPICLRLE-----------FERQQREELEKL 620

  Fly   550 GGSLNLYQDQQAALK------EQLSQEMVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHN 608
            .....|.::.:...|      |::.||:......::.:.|...|..|.||.   :|...:..|.:
Human   621 ESKRKLIEEMEEKQKSDKAELERMQQEVETQRKETEIVQLQIRKQEESLKR---RSFHIENKLKD 682

  Fly   609 KLIGEVMKISD---------------QHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEENNQRH 658
             |:.|..|..:               |..:.|: ::.|::|:.:.|.:.|.....|:.:|.:|..
Human   683 -LLAEKEKFEEERLREQQEIELQKKRQEEETFL-RVQEELQRLKELNNNEKAEKFQIFQELDQLQ 745

  Fly   659 K-------------------------------AMLDSMQEKFATIIDSSLQSVEEHAKQMH---- 688
            |                               ..|...||....:....:|.|||..:.:.
Human   746 KEKDEQYAKLELEKKRLEEQEKEQVMLVAHLEEQLREKQEMIQLLRRGEVQWVEEEKRDLEGIRE 810

  Fly   689 --KKLEQLGAMSLPDAEELQNLQ-----------EELAN-ERALAQQEDALLESMMMQMEQIKNL 739
              .::::..|....|.|||:..|           .:|.| |:.|.||:|.|.:.:..:.|.::.|
Human   811 SLLRVKEARAGGDEDGEELEKAQLRFFEFKRRQLVKLVNLEKDLVQQKDILKKEVQEEQEILECL 875

  Fly   740 RSKNSISMSVHLNKMEESRLTRNH--RIDDIKSGIQDYQKLG----------------------- 779
            :.::.          :||||...|  .:.|:....||::|:.
Human   876 KCEHD----------KESRLLEKHDESVTDVTEVPQDFEKIKPVEYRLQYKERQLQYLLQNHLPT 930

  Fly   780 -IEASQSAQAELTSQMEAGMLCLD------------------QGVANCSMLQVHMKNLNQKYEKE 825
             :|..|.| .|:   ::.|.|.||                  |..||.:.||    .|...:|..
Human   931 LLEEKQRA-FEI---LDRGPLSLDNTLYQVEKEMEEKEEQLAQYQANANQLQ----KLQATFEFT 987

  Fly   826 TNENVGSVRVHHNQVEIICQESKQQLEAVQEKTEVNLEQMVDARQQ------LITEDRQRFIGHA 884
            .|......:|...:.||:....|||.||: |:....||:...|.|:      .|.|.||:.   |
Human   988 ANIARQEEKVRKKEKEILESREKQQREAL-ERALARLERRHSALQRHSTLGMEIEEQRQKL---A 1048

  Fly   885 TVATDLVQESNRQFSEHAEHQRQQLQICEQELVRF--QQSELKTYAPTGTTPSKRDFVYPRTLVA 947
            ::.:...::|..|.|..||.:..:.   :||.:.:  ||.:.|.|...|........:..:...:
Human  1049 SLNSGSREQSGLQASLEAEQEALEK---DQERLEYEIQQLKQKIYEVDGVQKDHHGTLEGKVASS 1110

  Fly   948 TSP--------------------HQEIVRRYRQELDWSDLDTTATIDE-CSEGEHDVSMHSVQEL 991
            :.|                    .:|:.||.:        |....|.| ||.        |...:
Human  1111 SLPVSAEKSHLVPLMDARINAYIEEEVQRRLQ--------DLHRVISEGCST--------SADTM 1159

  Fly   992 SETETIMNST-------------------PIEPVDGVTVKRGCGTTR---NSNSNALKPPV--AT 1032
            .:.|.:.|.|                   |:.|.|...........|   :.|.:...|.:  .:
Human  1160 KDNEKLHNGTIQRKLKYEKRQFCPAWYLDPLLPRDATDTSSLIKEERREVDQNDHWDHPMLWSVS 1224

  Fly  1033 GGKRSSSLSRSLTPSKTSPRGSPAFVRHNKEN 1064
            |.|.:||.|:..|..:......|. |..:.||
Human  1225 GPKTTSSASQERTHQQRDQSSVPE-VFQDSEN 1255

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	147/379 (39%)
		SMC_prok_B	<408..728	CDD:274008	82/469 (17%)
		SMC_prok_B	<651..>921	CDD:274008	76/370 (21%)
		Microtub_bind	923..>1004	CDD:464048	17/120 (14%)
KIF16B	XP_005260807.1	KISc_KIF1A_KIF1B	2..365	CDD:276816	146/377 (39%)
		Kinesin_assoc	364..476	CDD:465047	19/112 (17%)
		FHA_KIF16B	446..562	CDD:438784	14/118 (12%)
		Smc	<606..>1089	CDD:440809	105/523 (20%)