Protein Alignment Klp61F and Kif14

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_006529793.2
• Gene: Kif14 / 381293
• MGIID: 1098226
• Length: 1717
• Species: Mus musculus
• Alignment Length: 1255
• Identity: 318/1255 - (25%)
• Similarity: 514/1255 - (40%)
• Gaps: 324/1255 - (25%)

  Fly     8 TSRQPQ--KKSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSF 70
            |..:|.  |..|..:.|.|||||.:.||:..::::||...| .|:...|. |.|....|.:|.||
Mouse   380 TFTEPDSLKVENSQVTVAVRVRPFSKREKTEKASQVVFTNG-EEITVEHP-DMKQVYSFIYDVSF 442

  Fly    71 GP-------ESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVG-NETAELKSSWED 127
            ..       .:.|..||..:.:||::....|||..:|||||||:||::||:| ||
Mouse   443 WSFDECHPGYASQTTVYETLAAPLLDRAFEGYNTCLFAYGQTGSGKSYTMMGLNE---------- 497

  Fly   128 DSDIGIIPRALSHLFDEL---RMMEVEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGS 189
              :.|||||....||.::   :..||.|.:.:|:.|:|||::.|||........|......::..
Mouse   498 --EPGIIPRFCEDLFAQIAKKQTSEVSYHLEMSFFEVYNEKIHDLLVCKGENGQRKQPLRAREHP 560

  Fly   190 V---IIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVV-HIRENGIEGE- 249
            |   .::||....|.|..|:...||.|.::|.||.|.||.:|||||:||::|: ..:...:|||
Mouse   561 VSGPYVEGLSMNVVSSYSDIQSWLELGNKQRATAATGMNDKSSRSHSVFTLVMTQTKTEVVEGEE 625

  Fly   250 -DMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVDRA----PHVPYR 309
             |.....::|||||||||..|.| :..|.|::|.|:||:||||||:||:||.::|    ..:|||
Mouse   626 HDHRITSRINLVDLAGSERCSTA-HSSGQRLKEGVSINKSLLTLGKVISALSEQANGKRVFIPYR 689

  Fly   310 ESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKE 374
            ||.||.||:|||||.:||::|||:||...:||||||||.||.:|:.|.|..:||:.:..| :::|
Mouse   690 ESTLTWLLKESLGGNSKTAMIATVSPAASNIEETLSTLRYATQARLIVNIAKVNEDMNAK-LIRE 753

  Fly   375 YTEEIDKLKRDLMAAR--DKNGIYLAEETYGEITLKLESQNRELNEKMLLLKALKDELQNKEKIF 437
            ...||:|||....:.|  |.....|..:....:.:||..|.|::.|   :.:..|::.:..||
Mouse   754 LKAEIEKLKAAQRSNRNIDPERYRLCRQEITSLRMKLHQQERDMAE---IQRVWKEKFEQAEK-- 813

  Fly   438 SEVSMSLVEKTQELKKTE---------ENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTE 493
                 ..:::|:||:|..         .||:|......|::.:|...    ||....|..|..:.
Mouse   814 -----RKLQETKELQKAGVTFQMDNHLPNLVNLNEDPQLSEMLLYMV----KEGVTTVGKHTPSS 869

  Fly   494 QVLTTQAQEILAAADLAT--------------------DDTHQLHGTIERRRELDEKIRRSCDQF 538
            . ...|...:|.|.|..|                    :.||....|:...   .:::....|.:
Mouse   870 S-HDIQLSGVLIADDHCTIRNFGGTVSIVPAGEAKTYVNGTHISEPTVLHH---GDRVVLGGDHY 930

  Fly   539 -------------KDRMQDNLEMIGGSLNL------------------YQDQQAALKEQLSQEMV 572
                         |...::||....|..:.                  .:|.|...||::.|
Mouse   931 FRFNHPVEVQKGKKLSSRNNLTTSEGPKDFEFAKNELLTAQRSRLEAEIKDAQLKAKEEMMQ--- 992

  Fly   573 NSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQQQ 637
                           .|::.|||..|.|..|..::.:.|           ||..|:|.|:.|:::
Mouse   993 ---------------GIQIAKEMAQQELSSQKAVYERKI-----------QALEAELREESQRKR 1031

  Fly   638 L--LMSKEIQTNLQVIEENNQR-------HKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQ 693
            |  |.:::....::.:|...|.       :|..|:  .|..||     .|::|:|..:..:.||.
Mouse  1032 LEELNNQKASHKIEELERAKQHLEQEVYVNKRRLE--METLAT-----KQALEDHRIRHARILEA 1089

  Fly   694 LGAMSLPDAEELQNLQEELAN-ERALAQQ---EDALLESMMMQMEQIKNLRSKNSISMSVHLNKM 754
            |.......|||:|.|||...| ::....|   ....|.:|:.:...|.: :.|.......| :..
Mouse  1090 LEIEKQKIAEEVQMLQENRGNRDKTFTIQPNWNSMKLSTMIQEANAISD-KFKKCYIFGRH-DAS 1152

  Fly   755 EESRLTRNHRIDDIKSGIQDYQKL-GIEASQSAQAEL---------------------------- 790
            ::.|...:.|:.:::.||..:..| ..|:..:|..||
Mouse  1153 DKGRSDTSVRVRNLQLGISTFWSLEKFESKLAAMKELYESNGGDRDEDVFCDPADEWEPDITSTP 1217

  Fly   791 ---TSQMEAGMLCLDQGVANC--------SMLQVHMKNLNQK----YEKET--------NENVGS 832
               .|:..:..|..::.|:.|        ||...|...|.:|    |...:        .|.:||
Mouse  1218 VSSLSRRRSRSLMKNRRVSGCLHDIHPIQSMQSSHSSGLMEKPSTIYSNSSESFLPGICKELIGS 1282

  Fly   833 -----------------------VRVHHNQVEIICQESKQQLEAVQEKTEVNL----------EQ 864
                                   :|:|:..:.|    ||.. |...|:::.||          .|
Mouse  1283 SIDFLGQSFDEEKTIADSLINNLLRLHNGVIAI----SKAH-EEQDEESQDNLFSDRAAQALTIQ 1342

  Fly   865 MVDARQQLIT------------------EDR------------QRFI-GHATVATDLVQESNRQF 898
            :..|.:||:.                  ||.            |.|: |..:..:.:|:|:..:.
Mouse  1343 VACAFEQLVVLFKHWLGDFLPCTGSARLEDELRQDIKKLGGYLQLFLQGCCSDISSMVKEAQNKV 1407

  Fly   899 SEHAEHQRQQLQICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIVRRYRQELD 963
            .:..    ||...|..:|...:.|:|.....:....|.::|:.......||..:.:       ||
Mouse  1408 MKII----QQAVQCVGQLAVLKGSKLCVLENSSKVSSTQEFMAALQDGVTSGMKSL-------LD 1461

  Fly   964 WSDLDTTATIDECSEGEHDVSMHSVQELSETETIMNSTPIEPVDGVTVKRGCGTTRNS----NSN 1024
             |.|:|...:      ..|:|..|.:|  |....|.::.:|.|         |:..|:    .:.
Mouse  1462 -SGLETAQDL------RQDLSRQSARE--EVTKQMKASTVEWV---------GSLENAVAEWRTK 1508

  Fly  1025 ALKPPVATGGKRSSSLSRSL 1044
            :.:.....|.::..|...||
Mouse  1509 SFRTQAQEGSRQQVSKLLSL 1528

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	152/368 (41%)
		SMC_prok_B	<408..728	CDD:274008	78/392 (20%)
		SMC_prok_B	<651..>921	CDD:274008	73/396 (18%)
		Microtub_bind	923..>1004	CDD:464048	16/80 (20%)
Kif14	XP_006529793.2	KISc_KIF1A_KIF1B	392..743	CDD:276816	149/365 (41%)
		Kinesin_assoc	740..859	CDD:465047	31/133 (23%)
		FHA_KIF14	829..936	CDD:438759	18/114 (16%)
		SMC_prok_B	<937..>1109	CDD:274008	45/207 (22%)