Protein Alignment Klp61F and KIF5A

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_004975.2
• Gene: KIF5A / 3798
• HGNCID: 6323
• Length: 1032
• Species: Homo sapiens
• Alignment Length: 1138
• Identity: 292/1138 - (25%)
• Similarity: 486/1138 - (42%)
• Gaps: 285/1138 - (25%)

  Fly    19 NIQVYVRVRPLNSRERCIRSAEVVDVV-GPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSV 82
            :|:|..|.||||..| .:|..:.:.:. |...||..       .|.:.|||.|.|.:.|..||..
Human     9 SIKVLCRFRPLNQAE-ILRGDKFIPIFQGDDSVVIG-------GKPYVFDRVFPPNTTQEQVYHA 65

  Fly    83 VVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRM 147
            ....::::||.|||.|:||||||.:||||||.|        ...|...:|||||....:|:.:..
Human    66 CAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEG--------KLHDPQLMGIIPRIARDIFNHIYS 122

  Fly   148 ME--VEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLL 210
            |:  :|:.:::||.|:|.:::.|||   |.||..:.....|.....::|..|..|.|.:::..::
Human   123 MDENLEFHIKVSYFEIYLDKIRDLL---DVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVI 184

  Fly   211 EKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKAGNEK 275
            ::||..|..|.|.||..|||||::|  :::|::..:|.|..|. |||.||||||||.|||.|.|
Human   185 DEGKSNRHVAVTNMNEHSSRSHSIF--LINIKQENMETEQKLS-GKLYLVDLAGSEKVSKTGAE- 245

  Fly   276 GIRVRETVNINQSLLTLGRVITALVD-RAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKD 339
            |..:.|..|||:||..||.||:||.: ...:||||:||:||:||:||||..:|::....||...:
Human   246 GAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYN 310

  Fly   340 IEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLK--RDLMA------ARDKN--- 393
            ..||.|||.:..|||.|:|...||.:||.:...|:|.:|.:|.|  ::.:|      :|.:|
Human   311 DAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGEN 375

  Fly   394 --------------GIYLAEET----YGEITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEV 440
                          |..|.|||    ...|.:::..:.|:..|:.  ::.|..:|.:|:...::.
Human   376 VPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEE--IRRLYKQLDDKDDEINQQ 438

  Fly   441 SMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTEQVLTTQAQEILA 505
            |..:.:..|::...||.|::|:|.               .||.:...||:::|.
Human   439 SQLIEKLKQQMLDQEELLVSTRGD---------------NEKVQRELSHLQSEN----------- 477

  Fly   506 AADLATDDTHQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQE 570
              |.|.|:..::...:|   ||.....:...:.:::.|.|               ..|.::|||:
Human   478 --DAAKDEVKEVLQALE---ELAVNYDQKSQEVEEKSQQN---------------QLLVDELSQK 522

  Fly   571 MVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQ 635
                  |:..|:|.|.          .|.||:.:....|.|.||:.           .||:.:.:
Human   523 ------VATMLSLESE----------LQRLQEVSGHQRKRIAEVLN-----------GLMKDLSE 560

  Fly   636 QQLLMSK-EIQTNLQV---IEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQM-------HK 689
            ..:::.. ||:..:::   |||.        .::...:.:.|.|.::||.:..:|:       |:
Human   561 FSVIVGNGEIKLPVEISGAIEEE--------FTVARLYISKIKSEVKSVVKRCRQLENLQVECHR 617

  Fly   690 KLEQLGAMSLPDAEELQNLQEELANERALAQQEDALLESM--MMQMEQIKNLRSKNSI-SMSVHL 751
            |:|..|              .||::.:.|..|.:|.:.|:  .||..::|....:.|. |:|..|
Human   618 KMEVTG--------------RELSSCQLLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDEL 668

  Fly   752 NKMEESRLTRNHRI------------DDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQG 804
            .|::.....  |.:            |::|..::...:...||.....|.|..::......:|:.
Human   669 AKLQAQETV--HEVALKDKEPDTQDADEVKKALELQMESHREAHHRQLARLRDEINEKQKTIDEL 731

  Fly   805 VANCSMLQVHMKNLNQKYEK-ETNENVGSVRV--------HHNQVEIICQESKQQLEAVQEKTEV 860
            ......||:.::.|...||| ::.|:..|.::        .|.|       |||.|:.::|....
Human   732 KDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLYERHEQ-------SKQDLKGLEETVAR 789

  Fly   861 NLEQMVDARQQLITEDRQRFIGHATVATDLVQE------SNRQFSEHAEHQRQQLQICEQELVRF 919
            .|:.:.:.|:..:.:...|    ...:.::..|      |.:|.....|:..:||....::|||
Human   790 ELQTLHNLRKLFVQDVTTR----VKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVR- 849

  Fly   920 QQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIV---------------RRYRQELDWSDLDT 969
            ..::|:...|.          ..:.|.||:...:.:               |||:||:|
Human   850 DNADLRCELPK----------LEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVD------ 898

  Fly   970 TATIDEC----SEGEHDVSMHSVQELSETETIMNSTPIEPVDGVTVKRGCGTTRNSNSNALKP-- 1028
              .|.|.    |.|:..   ||.|         .:.|:.|               .:..|..|
Human   899 --RIKEAVRYKSSGKRG---HSAQ---------IAKPVRP---------------GHYPASSPTN 934

  Fly  1029 PVATGGKRSSSLSRSL-----------TPSKTS 1050
            |..|......|.:.||           |||.||
Human   935 PYGTRSPECISYTNSLFQNYQNLYLQATPSSTS 967

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	139/349 (40%)
		SMC_prok_B	<408..728	CDD:274008	61/330 (18%)
		SMC_prok_B	<651..>921	CDD:274008	61/306 (20%)
		Microtub_bind	923..>1004	CDD:464048	19/99 (19%)
KIF5A	NP_004975.2	KISc_KHC_KIF5	7..327	CDD:276820	135/340 (40%)
		Microtubule-binding	174..315		66/144 (46%)
		Necessary for interaction with ZFYVE27. /evidence=ECO:0000250|UniProtKB:P33175	271..361		36/89 (40%)
		Interaction with BICD2. /evidence=ECO:0000269|PubMed:20386726	353..1032		147/771 (19%)
		PRK03918	<409..920	CDD:235175	123/651 (19%)
		Khc_CBD_cc	832..901	CDD:467880	17/87 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	906..939		10/59 (17%)
		Globular	907..1032		19/88 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	978..1010