Protein Alignment Klp61F and Kif5a

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_997688.1
• Gene: Kif5a / 314906
• RGDID: 1303035
• Length: 1027
• Species: Rattus norvegicus
• Alignment Length: 1140
• Identity: 289/1140 - (25%)
• Similarity: 486/1140 - (42%)
• Gaps: 284/1140 - (24%)

  Fly    19 NIQVYVRVRPLNSRERCIRSAEVVDVV-GPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSV 82
            :|:|..|.||||..| .:|..:.:.:. |...|:..       .|.:.|||.|.|.:.|..||..
  Rat     9 SIKVLCRFRPLNQAE-ILRGDKFIPIFQGDDSVIIG-------GKPYVFDRVFPPNTTQEQVYHA 65

  Fly    83 VVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRM 147
            ....::::||.|||.|:||||||.:||||||.|        ...|...:|||||....:|:.:..
  Rat    66 CAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEG--------KLHDPQLMGIIPRIARDIFNHIYS 122

  Fly   148 ME--VEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLL 210
            |:  :|:.:::||.|:|.:::.|||   |.||..:.....|.....::|..|..|.|.:::..::
  Rat   123 MDENLEFHIKVSYFEIYLDKIRDLL---DVTKTNLSVHEDKNRVPFVRGCTERFVSSPEEILDVI 184

  Fly   211 EKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKAGNEK 275
            ::||..|..|.|.||..|||||::|  :::|::..||.|..|. |||.|.||||||.|||.|.|
  Rat   185 DEGKSNRHVAVTNMNEHSSRSHSIF--LINIKQENIETEQKLS-GKLYLADLAGSEKVSKTGAE- 245

  Fly   276 GIRVRETVNINQSLLTLGRVITALVD-RAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKD 339
            |..:.|..|||:||..||.||:||.: ...:||||:||:||:||:||||..:|::....||...:
  Rat   246 GAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYN 310

  Fly   340 IEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEE----------IDKLKRDLMAARDKNG 394
            ..||.|||.:..|||.|:|...||.:||.:...|:|.:|          |.||:.:|...|:...
  Rat   311 DAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGEN 375

  Fly   395 IYLAEETYGE-------------------ITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEV 440
            :...|...||                   |.:::..:.|:..|:.  ::.|..:|.:|:...::.
  Rat   376 VPETERLAGEDSALAAEICEETPVNDNSSIVVRIAPEERQKYEEE--IRRLYKQLDDKDDEINQQ 438

  Fly   441 SMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTE-QVLTTQAQEIL 504
            |..:.:..|::...||.|::|:|.               .||.:...||:::| .....:.:|:|
  Rat   439 SQLIEKLKQQMLDQEELLVSTRGD---------------NEKVQRELSHLQSENDAAKEEVKEVL 488

  Fly   505 -AAADLATDDTHQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLS 568
             |..:||.:..       ::.:|::||                          ..|...|.::||
  Rat   489 QALEELAVNYD-------QKSQEVEEK--------------------------SQQNQLLVDELS 520

  Fly   569 QEMVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQM 633
            |:      |:..|:|.|.          .|.||:.:....|.|.||:.           .||:.:
  Rat   521 QK------VATMLSLESE----------PQRLQEVSGHQRKRIAEVLN-----------GLMKDL 558

  Fly   634 QQQQLLMSK-EIQTNLQV---IEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQM------- 687
            .:..:::.. ||:..:::   |||.        .::...:.:.|.|.::||.:..:|:
  Rat   559 SEFSVIVGNGEIKLPVEISGAIEEE--------FTVARLYISKIKSEVKSVVKRCRQLENLQVEC 615

  Fly   688 HKKLEQLGAMSLPDAEELQNLQEELANERALAQQEDALLESM--MMQMEQIKNLRSKNSI-SMSV 749
            |:|:|..|              .||::.:.|..|.:|.:.|:  .||..::|....:.|. |:|.
  Rat   616 HRKMEVTG--------------RELSSCQLLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSD 666

  Fly   750 HLNKMEESRLTRNHRIDDIKSGIQDYQ------KLGIEASQSAQ----AELTSQMEAGMLCLDQG 804
            .|.|::.........:.|.:...||.:      :|.:|..:.|.    |.|..::......:|:.
  Rat   667 ELAKLQAQETVHEVALKDKEPDTQDAEEVKKALELQMENHREAHHRQLARLRDEINEKQKTIDEL 731

  Fly   805 VANCSMLQVHMKNLNQKYEKETN-ENVGSVRV--------HHNQVEIICQESKQQLEAVQEKTEV 860
            ......||:.::.|...||:..| ||..|.::        .|.|       |||.|:
  Rat   732 KDLDQKLQLELEKLQADYERLKNEENEKSAKLQELTFLYERHEQ-------SKQDLK-------- 781

  Fly   861 NLEQMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELK 925
            .||:.|....|.:...|:.|:...|.......|...:.|.....|:|::...|..|.:.
  Rat   782 GLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQL------ 840

  Fly   926 TYAPTGTTPSKRDFVYPRTLVATSPHQEIVRRYRQELDWSDLDTTATIDECSEGEHDVSMHSVQE 990
                                  |..|:::||      |.:||       .|             |
  Rat   841 ----------------------TEVHKQLVR------DNADL-------RC-------------E 857

  Fly   991 LSETETIMNSTP--IEPVDGVTVKRGCGTTRNSNS-----NALKPPV--ATGGKR--SSSLSRSL 1044
            |.:.|..:.:|.  ::.::|...:...|..::...     :.:|..|  .:.|||  |:.:::.:
  Rat   858 LPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPV 922

  Fly  1045 TP------SKTSPRG--SPAFVRH------NKENV 1065
            .|      |.|:|.|  ||..:.:      |.:|:
  Rat   923 RPGHYPASSPTNPYGTRSPECISYTNNLFQNYQNL 957

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	138/349 (40%)
		SMC_prok_B	<408..728	CDD:274008	62/332 (19%)
		SMC_prok_B	<651..>921	CDD:274008	63/298 (21%)
		Microtub_bind	923..>1004	CDD:464048	12/82 (15%)
Kif5a	NP_997688.1	KISc_KHC_KIF5	7..327	CDD:276820	134/340 (39%)
		Microtubule-binding. /evidence=ECO:0000255	174..315		66/144 (46%)
		Necessary for interaction with ZFYVE27. /evidence=ECO:0000250|UniProtKB:P33175	271..361		34/89 (38%)
		Interaction with BICD2. /evidence=ECO:0000250|UniProtKB:Q12840	353..1027		146/773 (19%)
		PRK03918	<409..920	CDD:235175	128/678 (19%)
		Khc_CBD_cc	832..901	CDD:467880	16/122 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	906..936		7/29 (24%)
		Globular. /evidence=ECO:0000255	907..1027		13/51 (25%)