Protein Alignment Klp61F and Kif20b

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_006231343.1
• Gene: Kif20b / 309523
• RGDID: 1308497
• Length: 1781
• Species: Rattus norvegicus
• Alignment Length: 1262
• Identity: 298/1262 - (23%)
• Similarity: 519/1262 - (41%)
• Gaps: 369/1262 - (29%)

  Fly    15 KSNQNIQVYVRVRPLNSRER------CIR--SAEVVDVVGPREVVTRHTLDS--KLTKKFTFDRS 69
            :|...:||.:|:||....|:      |::  .::.|.:..|:.::...:..|  ::.:||:|.|.
  Rat    54 ESKNYLQVCLRIRPFTQSEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRV 118

  Fly    70 FGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGII 134
            ||||:.|.:.:...:...::::|.|::..:|.||.|.:|||:|..|.|           .:|||:
  Rat   119 FGPETSQKEFFQGCIMQPVKDLLKGHSRLIFTYGLTNSGKTYTFQGTE-----------ENIGIL 172

  Fly   135 PRALSHLFDELR----------------------------------------------------- 146
            ||.|:.|||.|:
  Rat   173 PRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEKEESANKNTLLRQIKEVTIHNDSYDILC 237

  Fly   147 -------------------------MMEVEYTMRISYLELYNEELCDLL-----STDDTTKIRIF 181
                                     :..::|::.:|:.|:|||.:.||.     .......:|:.
  Rat   238 GRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNESIYDLFVPVSSKLQKRKMLRLS 302

  Fly   182 DDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSI-VVHIRENG 245
            .|  .||...|:.|:.|.|....:.|:||:.|.:.:..|.|.:|..|||||::|:| ::.|.::.
  Rat   303 QD--VKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRSHSIFTIRILQIEDSE 365

  Fly   246 IEGEDMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITAL--VDRA---PH 305
            |  ..:.::.:|:|.||||||...|..:| |.|:||..|||.||||||:.|:.|  .|::   .|
  Rat   366 I--PRVTRVSELSLCDLAGSERSMKTQSE-GERLREAGNINTSLLTLGKCISVLKNSDKSKIQQH 427

  Fly   306 VPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAK---------------- 354
            ||:||||||...|....|:.|..:|..||......:|||:.|:::..|:
  Rat   428 VPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEKSF 492

  Fly   355 -------------NIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETYG-EI 405
                         |:.|| .:|.|  :|||..|.:.|......||:...::|     |||.. |.
  Rat   493 GSTKSLQDVSLGSNLDNK-ILNVK--RKTVSWENSLEDVVENEDLVEDLEEN-----EETQNMET 549

  Fly   406 TLKLESQNREL-----------NEKML-LLKALKDELQN--KEKIFSEVSMSLVEKTQELKK-TE 455
            .|..|..::.|           |:|:| |::.||..|.|  |||:..|..:. .|.|||..: ..
  Rat   550 ELTDEDSDKPLEEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIR-EEVTQEFTQYWS 613

  Fly   456 ENLLNTKGTLLLTKKVLTK-TKRRYKEKKELV----------ASHMKTEQVLTTQAQEILAAADL 509
            :...:.|.|||..:::|.: .:||....|:||          ||...|.::.|.::...:...|:
  Rat   614 QREADFKETLLHEREILEENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEESHNYVGVEDI 678

  Fly   510 ---ATDDTHQLHGTIERRREL---------DEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAA 562
               ..||...    |:::.||         |.:...:|.|.|.. |...|:......|.:.|: .
  Rat   679 FDSLEDDVTD----IKKQAELAHLYITSLVDPQEAIACLQLKYN-QVKAELAETKEELIKAQE-E 737

  Fly   563 LK----EQLSQEMVNSSYVSQRLALNSS---KSIEMLKEMCAQSLQDQTNLHNK--LIGE----- 613
            ||    :.|.|.:..||.|...|..|.|   ::.||.|:...|:..::..|:..  .:||
  Rat   738 LKNKESDSLVQALKTSSKVDTSLISNKSTGNETTEMPKKSRTQTHSERKRLNEDGLQLGEPPAKK 802

  Fly   614 -VMKIS-----DQHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEENNQRHKAMLDSMQEKFATI 672
             ::.||     ||..:       |:|||.   :|:..:.:.:|::|.|:..|.:|        ||
  Rat   803 GLILISPPITEDQDKR-------EEMQQS---VSEGAEEDSRVLQEKNEELKRLL--------TI 849

  Fly   673 IDSSLQSVEEHAKQMHKKL----------EQLGAMSLPDAEELQNLQE----ELANERALAQ-QE 722
            .::.|::.:|...:::|::          |:..:....:.|::|...:    ||..:||:.| |:
  Rat   850 GENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQEQK 914

  Fly   723 DALLE----------------SMMMQME-QIKNLRSKNSISMSVHLNKMEESRLTRNHRIDDIKS 770
            |.:|:                |.:.||: :|..|||.:|.|   |::|::...|      .|:.|
  Rat   915 DRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPS---HISKIDLLNL------QDLSS 970

  Fly   771 GIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGVANCSMLQVHMKNLN-QKYEKETNENVGSVR 834
            |                        |.:|...|.:....:....:|..: |:..:|::
  Rat   971 G------------------------ANLLNTSQQLPGSDLPSTWVKEFHTQELSRESS------- 1004

  Fly   835 VHHNQVEIICQESK-------------QQLEAVQEKTEVNLEQMVDARQQL---ITEDRQRFIGH 883
             .|:.:|.|.:|.|             |.||.:.||.:|.::...|...:|   .:||:.| ...
  Rat  1005 -FHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENSELRAKESEDKNR-DQQ 1067

  Fly   884 ATVATDLVQESNRQFSEHAEHQRQQLQ-ICEQE----------------------LVRFQQSELK 925
            ......|:|:...:..|.....|.|:| :.|:|                      :|..|:.|.|
  Rat  1068 LKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEVMVETQKEECK 1132

  Fly   926 TYAPTGTTPSKRDFVYPRTLVAT-----SPHQEIVR----------RYRQELDW--SDLDTTATI 973
            ..|....:..:::....: |.|:     :.||:.:|          ::|:|:..  ::|..|..:
  Rat  1133 RLAELEQSILEKESAILK-LEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHDTKQL 1196

  Fly   974 DECSEGEHDVSMHSVQELSETETIMNS 1000
            .:..|.|:::|....::|.| |...||
  Rat  1197 LQSKEEENEISRQETEKLKE-ELAANS 1222

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	123/475 (26%)
		SMC_prok_B	<408..728	CDD:274008	93/408 (23%)
		SMC_prok_B	<651..>921	CDD:274008	66/341 (19%)
		Microtub_bind	923..>1004	CDD:464048	20/95 (21%)
Kif20b	XP_006231343.1	KISc_KIF23_like	58..476	CDD:276819	118/433 (27%)
		RBD_KIF20B	599..647	CDD:409644	14/48 (29%)
		SMC_prok_B	675..1490	CDD:274008	128/616 (21%)