Protein Alignment Klp61F and Kif21a

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_038934809.1
• Gene: Kif21a / 300158
• RGDID: 1305126
• Length: 1677
• Species: Rattus norvegicus
• Alignment Length: 1107
• Identity: 284/1107 - (25%)
• Similarity: 485/1107 - (43%)
• Gaps: 269/1107 - (24%)

  Fly    19 NIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSVV 83
            :::|.||:||..::|: |....:...|.|.|.......|    |.||||..|..:|:|..:|:..
  Rat     9 SVRVAVRIRPQLAKEK-IEGCHICTSVTPGEPQVFLGKD----KAFTFDYVFDIDSQQEQIYTQC 68

  Fly    84 VSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRMM 148
            :..|||....|||.|||||||||.|||:||.......:.     :.:.|||.||:.|||..:...
  Rat    69 IEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIM-----EEEQGIISRAVKHLFKSIEEK 128

  Fly   149 EV----------EYTMRISYLELYNEELCDLLSTDDTTK----------IRIFDDSTKKGSVIIQ 193
            :.          |:.:...:|||||||:.||.   |||:          |||.:|||  |.:...
  Rat   129 KTSAIKNGVPPPEFKVNAQFLELYNEEVLDLF---DTTRDIDAKNKKSNIRIHEDST--GGIYTV 188

  Fly   194 GLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVV------------HIRENGI 246
            |:....|:::.::.:.|:.|...|.||:|.||.||||||.:|:|.|            ::.:|.:
  Rat   189 GVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKM 253

  Fly   247 --EGEDMLKI----GKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVD---R 302
              |...|.:.    .|.:.|||||||.:.:.| ..|.|.:|.::||..||.||.||:||.|   |
  Rat   254 VSESPQMNEFETLTAKFHFVDLAGSERLKRTG-ATGERAKEGISINCGLLALGNVISALGDKSKR 317

  Fly   303 APHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLT 367
            |.|||||:||||||||:||||.::|.:||.:||..:|..|||:||:||:||:||:||..|||...
  Rat   318 ATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRA 382

  Fly   368 KKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETYGEITLKLESQNRELNEKMLLLKALKDELQN 432
            .:.: .....||.:|:.:||  ..|.|..:.:|.      .:||.|...:|..:|
  Rat   383 SQQI-NALRSEITRLQMELM--EYKTGKRIIDEE------GVESINDMFHENAML---------- 428

  Fly   433 KEKIFSEVSMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELV-ASHMKTEQVL 496
                                :||.|.|..                |.|..:|.| |...:..|::
  Rat   429 --------------------QTENNNLRV----------------RIKAMQETVDALRARITQLV 457

  Fly   497 TTQAQEILAAADLATDD-THQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQ 560
            :.||.::||.|....:: ::.:|..|:...:|..|:..|     :.:.:|
  Rat   458 SEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLES-----EAVNEN--------------- 502

  Fly   561 AALKEQLSQEMVNSSYVSQRLALNSS------KSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISD 619
              |::.|::....|.:.|...|.:|:      ::||:: ::..:.|            |.:|..:
  Rat   503 --LRKSLTRATARSPFFSASSAFSSTILSSDKETIEII-DLAKKDL------------EKLKRKE 552

  Fly   620 QHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEEN------NQRHKAMLDSMQEKFATIIDSSLQ 678
            :..:..:.||.|..::::.:..||  .|....:|.      :::..:.:|..:.:.|:..:....
  Rat   553 KRKKKRLQKLEESSREERSVAGKE--NNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEED 615

  Fly   679 SVEEHAKQMHKKLEQLGAMSLPDAEELQNLQEELAN---ERALAQQEDALLESMMMQMEQIKNLR 740
            ..||..::...:.|:....|..:::|..|.|.:|||   |.|:.|:   |::.:....::::.|:
  Rat   616 EEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQK---LIDELENSQKRLQTLK 677

  Fly   741 SKNSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGV 805
            .:....:.:..:|:.:::|.|:..:.::.| ::.|.:   |.::..:.|...::.|
  Rat   678 KQYEEKLMMLQHKIRDTQLERDQVLQNLGS-VESYSE---EKAKKVKCEYEKKLHA--------- 729

  Fly   806 ANCSMLQVHMKNLNQKYEKETNENVGSVRVHHNQVEIICQESKQQLEAVQ-EKTEVNL-EQMVDA 868
                        :|::.::.........|:..||.:...|..|.|.:..: :||:|.| :||.:.
  Rat   730 ------------MNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEE 782

  Fly   869 RQQL-ITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTYAPTGT 932
            :::. :||.|:                ||:.::..:.||
  Rat   783 QEKARLTESRR----------------NREIAQLKKDQR-------------------------- 805

  Fly   933 TPSKRDFVYPRTLVATSPHQEIVRRYRQELDWSDLDTTATIDECSEGEHDVSMHSVQELSETETI 997
               |||... |.|.|...:||:|.|.:.|      :.||...:.......|:....::||.:|
  Rat   806 ---KRDHQL-RLLEAQKRNQEVVLRRKTE------EVTALRRQVRPMSDKVAGKVTRKLSSSE-- 858

  Fly   998 MNSTPIEPVDGVTVKRG-CGTTRNSNSNALKPPVA---------TGGKRSSSLSRSLT----PSK 1048
               :| .|..|.:|..| ...:|......::.|||         |.|.|.....:..|    .||
  Rat   859 ---SP-TPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSK 919

  Fly  1049 TS 1050
            |:
  Rat   920 TA 921

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	153/386 (40%)
		SMC_prok_B	<408..728	CDD:274008	60/336 (18%)
		SMC_prok_B	<651..>921	CDD:274008	46/281 (16%)
		Microtub_bind	923..>1004	CDD:464048	18/80 (23%)
Kif21a	XP_038934809.1	KISc_KIF4	8..372	CDD:276823	148/378 (39%)
		SCP-1	<389..1040	CDD:114219	130/710 (18%)
		EnvC	642..>853	CDD:443969	52/290 (18%)
		Rcc_KIF21A	938..1019	CDD:410204
		WD40	1342..1655	CDD:238121
		WD40 repeat	1393..1452	CDD:293791
		WD40 repeat	1458..1493	CDD:293791
		WD40 repeat	1498..1542	CDD:293791
		WD40 repeat	1550..1583	CDD:293791
		WD40 repeat	1590..1626	CDD:293791