DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp61F and KIF4B

DIOPT Version :9

Sequence 1:	NP_476818.1	Gene:	Klp61F / 38135	FlyBaseID:	FBgn0004378	Length:	1066	Species:	Drosophila melanogaster
Sequence 2:	NP_001092763.1	Gene:	KIF4B / 285643	HGNCID:	6322	Length:	1234	Species:	Homo sapiens

Alignment Length:	1234	Identity:	326/1234 - (26%)
Similarity:	530/1234 - (42%)	Gaps:	322/1234 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    20 IQVYVRVRPLNSRE------RCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCD 78
            ::|.:|.|||..:|      .|:..     |.|..:||.      ...|.||:|..|.|.::|.:
Human    10 VRVALRCRPLVPKEISEGCQMCLSF-----VPGETQVVV------GTDKSFTYDFVFDPCTEQEE 63

  Fly    79 VYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFD 143
            |::..|:|||:.:..|||.||.||||||:|||::|.|..|||    .|::..:|||||.:..||.
Human    64 VFNKAVAPLIKGIFKGYNATVLAYGQTGSGKTYSMGGAYTAE----QENEPTVGIIPRVIQLLFK 124

  Fly   144 EL-RMMEVEYTMRISYLELYNEELCDLL-STDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDV 206
            |: :..:.|:|:::||||:||||:.||| .:.:..:|.|.:| .|:|..|: ||.|..|....|.
Human   125 EIDKKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIRED-PKEGIKIV-GLTEKTVLVALDT 187

  Fly   207 YKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKA 271
            ...||:|...|..|:|.||:||||||.:|:|.:..|:.  ..::.....||:||||||||...|.
Human   188 VSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRKK--SDKNCSFRSKLHLVDLAGSERQKKT 250

  Fly   272 GNEKGIRVRETVNINQSLLTLGRVITALVD--RAPHVPYRESKLTRLLQESLGGRTKTSIIATIS 334
            ..| |.|::|.:|||:.||.||.||:||.|  :...||||:||||||||:||||.:.|.:||.:|
Human   251 KAE-GDRLKEGININRGLLCLGNVISALGDDKKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVS 314

  Fly   335 PGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAAR----DKNGI 395
            |...::|||||||.||.||:.|:|||.||        :..:|.|::.||:.:...:    ..:|.
Human   315 PADSNLEETLSTLRYADRARKIKNKPIVN--------IDPHTAELNHLKQQVQQLQVLLLQAHGG 371

  Fly   396 YLAEETYGEITLKLES---QNREL---NEKM--LLLKALKDELQNKEKIF--SEVSMSLVEKTQE 450
            .|......|.:..|:|   :|:.|   |||:  .|.||.....|..|:|.  .:|:..|..|.:|
Human   372 TLPGSINAEPSENLQSLMEKNQSLVEENEKLSRCLSKAAGQTAQMLERIILTEQVNEKLNAKLEE 436

  Fly   451 LKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTEQVLTTQAQEIL----AAADLAT 511
            |::      :....|.|.|.|.|...:..||..|::.:   .:|::|..:.|.:    ||.|.|.
Human   437 LRQ------HVACKLDLQKLVETLEDQELKENVEIICN---LQQLITQLSDETVACTAAAIDTAV 492

  Fly   512 DDTHQLHGTIERRRELD---------------------------EKIRRSCDQFKDRM------- 542
            ::..|:..:.|..|..|                           |.:.|...|..:::
Human   493 EEEAQVETSPETSRSSDAFTTQHALHQAQMSKEVVELNNALALKEALVRKMTQNDNQLQPIQFQY 557

  Fly   543 QDN-----LEMIGGSLNLYQDQQAALKE-QLSQEMVNSSYVSQ----------------RLALNS 585
            |||     ||:|    ||.::::..::| |.:::.||.:.:|:                :..||.
Human   558 QDNIKNLELEVI----NLQKEKEELVRELQTAKKNVNQAKLSEHRHKLLQELEGQIADLKKKLNE 618

  Fly   586 SKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQ-------------QQ 637
            ...:..|||...:::       :||..|:..:.:|..|     ||.||::             ::
Human   619 QSKLLKLKESTERTV-------SKLNQEIWMMKNQRVQ-----LMRQMKEDAEKFRQWKQKKDKE 671

  Fly   638 LLMSKE----IQTNLQVIEENNQRHKAMLDSMQEKFATI---IDSSLQSVEE-----HAKQMHKK 690
            ::..||    .|..|..:|.|.|:..::|....|:.|..   :..:||...|     ...|.|.|
Human   672 VIQLKERDRKRQYELLKLERNFQKQSSVLRRKTEEAAAANKRLKDALQKQREVTDKRKETQSHGK 736

  Fly   691 LEQLGA-----------MSLPDAEELQNLQEELANERALAQ------------------------ 720
             |.:.|           :.:...|..::|.:.|.:.:.|||
Human   737 -EGIAARVRNWLGNEIEVMVSTEEAKRHLNDLLEDRKILAQDVVQLKEKKESRENPPPKLRKCTF 800

  Fly   721 -----------QEDAL---LESMMMQME----QIKNLRSK------------------------- 742
                       .||.:   :||:..:||    ||.:|:.|
Human   801 SLSEVHGQVLESEDCITKQIESLETEMELRSAQIADLQQKLLDAESEDRPKQCWENIATILEAKC 865

  Fly   743 -------NSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLC 800
                   ..:|..:|:.|:|.|       :...|:...|.||:..| .|:..:|:.::::|.::.
Human   866 ALKYLIGELVSSKIHVTKLENS-------LRQSKASCADMQKMLFE-EQNHFSEIETELQAELVR 922

  Fly   801 LDQGVANCSMLQVHMKNLNQKYEKETNENVGSVRVHHNQVEIICQESKQQLEAVQEKTEVNLEQM 865
            ::|......:..|.....:|..||              |:|....|.:|||.:..:..:..||:|
Human   923 MEQQHQEKVLYLVSQLQESQMAEK--------------QLEKSASEKEQQLVSTLQCQDEELEKM 973

  Fly   866 VDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTYAPT 930
                                          |:..|..:...|:.:|.:|:|:..|.:..:.:.|.
Human   974 ------------------------------REVCEQNQQLLQENEIIKQKLILLQVASRQKHLPN 1008

  Fly   931 GT--TP-SKRDFVYPRTLVATSPHQEIVRRYRQELDWSDL----DTTATIDECSEGEHDVSMHSV 988
            .|  :| |..:::.|:    ..|.:...:...|.:|..||    :.:....|..:|:.|......
Human  1009 DTLLSPDSSFEYIPPK----PKPSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDGDSDEGDD 1069

  Fly   989 QELSETETIMNSTPIEPVDGVTVKRGCGTTRNSNSNALKPPVATGGKRSSSLSRSLTPSKTSPR 1052
            :|...|:.:..|.  :.:.|.:.|..||   |......|.      |....:..|..|:|...|
Human  1070 EEWKPTKLVKVSR--KNIQGCSCKGWCG---NKQCGCRKQ------KSDCGVDCSCDPTKCRNR 1122

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	157/354 (44%)
		Kinesin	25..356	CDD:278646	150/340 (44%)
		Microtub_bind	923..>1009	CDD:290642	16/92 (17%)
KIF4B	NP_001092763.1	KISc_KIF4	8..337	CDD:276823	151/346 (44%)
		KISc	9..343	CDD:214526	155/352 (44%)
		SbcC	406..995	CDD:223496	126/666 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	494..513		4/18 (22%)
		Interaction with PRC1. /evidence=ECO:0000250\|UniProtKB:O95239	663..1234		95/528 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	712..737		6/25 (24%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:O95239	793..798		0/4 (0%)
		Globular. /evidence=ECO:0000250	1000..1234		27/138 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1007..1030		6/26 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1052..1076		4/23 (17%)
		Pre-SET	<1072..1122	CDD:282838	12/60 (20%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000250\|UniProtKB:O95239	1086..1144		11/46 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1143		1/1 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1183..1234
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910