Protein Alignment Klp61F and KIF4A

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_036442.3
• Gene: KIF4A / 24137
• HGNCID: 13339
• Length: 1232
• Species: Homo sapiens
• Alignment Length: 1179
• Identity: 321/1179 - (27%)
• Similarity: 519/1179 - (44%)
• Gaps: 277/1179 - (23%)

  Fly    20 IQVYVRVRPLNSRE------RCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCD 78
            ::|.:|.|||..:|      .|:..     |.|..:||.      ...|.||:|..|.|.::|.:
Human    10 VRVALRCRPLVPKEISEGCQMCLSF-----VPGEPQVVV------GTDKSFTYDFVFDPSTEQEE 63

  Fly    79 VYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFD 143
            |::..|:|||:.|..|||.||.||||||:|||::|.|..|||    .|::..:|:|||.:..||.
Human    64 VFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAE----QENEPTVGVIPRVIQLLFK 124

  Fly   144 EL-RMMEVEYTMRISYLELYNEELCDLL-STDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDV 206
            |: :..:.|:|:::||||:||||:.||| .:.:..:|.|.:| .|:|..|: ||.|..|....|.
Human   125 EIDKKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIRED-PKEGIKIV-GLTEKTVLVALDT 187

  Fly   207 YKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKA 271
            ...||:|...|..|:|.||:||||||.:|:|.:..|:...:.....  .||:||||||||...|.
Human   188 VSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRKKSDKNSSFR--SKLHLVDLAGSERQKKT 250

  Fly   272 GNEKGIRVRETVNINQSLLTLGRVITALVD--RAPHVPYRESKLTRLLQESLGGRTKTSIIATIS 334
            ..| |.|::|.:|||:.||.||.||:||.|  :...||||:||||||||:||||.:.|.:||.:|
Human   251 KAE-GDRLKEGININRGLLCLGNVISALGDDKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVS 314

  Fly   335 PGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAAR----DKNGI 395
            |...::||||:||.||.||:.|:|||.||        :...|.|::.||:.:...:    ..:|.
Human   315 PADSNLEETLNTLRYADRARKIKNKPIVN--------IDPQTAELNHLKQQVQQLQVLLLQAHGG 371

  Fly   396 YLAEETYGEITLK-------LESQNREL---NEKML--LLKALKDELQNKEKIFSEVSMSLVEKT 448
            .|.    |.||::       |..:|:.|   |||:.  |.:|.....|..|:|.      |.|:.
Human   372 TLP----GSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERII------LTEQA 426

  Fly   449 QEL--KKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTEQVLTTQAQE----ILAAA 507
            .|.  .|.||...:....|.|.|.|.|...:..||..|::.:   .:|::|..:.|    :.||.
Human   427 NEKMNAKLEELRQHAACKLDLQKLVETLEDQELKENVEIICN---LQQLITQLSDETVACMAAAI 488

  Fly   508 DLATDDTHQLHGTIERRRELD---------------------------EKIRRSCDQFKDRM--- 542
            |.|.:...|:..:.|..|..|                           |.:.|...|...::
Human   489 DTAVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPI 553

  Fly   543 ----QDN-----LEMIGGSLNLYQD-QQAALKEQLSQEMVNSSYVSQR----------------L 581
                |||     ||:|    ||.:: ::..|:.|.:::..|.:.:|:|                .
Human   554 QYQYQDNIKELELEVI----NLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKK 614

  Fly   582 ALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQ-----QQLLMS 641
            .||....:..|||...:::       :||..|:..:.:|..|     ||.||::     :|....
Human   615 KLNEQSKLLKLKESTERTV-------SKLNQEIRMMKNQRVQ-----LMRQMKEDAEKFRQWKQK 667

  Fly   642 KEIQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSLPDAEELQ 706
            |:.:. :|:.|.:.:|...:| .::..|           ::.:..:.:|.|:..|.:....:.||
Human   668 KDKEV-IQLKERDRKRQYELL-KLERNF-----------QKQSNVLRRKTEEAAAANKRLKDALQ 719

  Fly   707 NLQEELANERALAQQEDALLESMMMQMEQIKNLRSKNSISMSV-------HLNKMEESRLTRNHR 764
            . |.|:|::|...|.     ..|.....::||... |.|.:.|       |||.:.|.|......
Human   720 K-QREVADKRKETQS-----RGMEGTAARVKNWLG-NEIEVMVSTEEAKRHLNDLLEDRKILAQD 777

  Fly   765 IDDIK----SGIQDYQKLGIEA-------SQSAQAE--LTSQMEAGMLCLDQGVANCSMLQVHMK 816
            :..:|    ||.....||....       .|.:::|  :|.|:|:  |..:....:..:..:..|
Human   778 VAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIES--LETEMEFRSAQIADLQQK 840

  Fly   817 NLNQKYE---KETNENVGSVRVHHNQVEIICQ---------ESKQQLEAVQEKTEVNLEQMVDAR 869
            .|:.:.|   |:..||:.::      :|..|.         .||.|:..::...:.:.....| .
Human   841 LLDAESEDRPKQRWENIATI------LEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCAD-M 898

  Fly   870 QQLITEDRQRFIGHATVATDLVQE------------------------SNRQFSEHAEHQRQQL- 909
            |:::.|:|..|   |.:.|:|..|                        :.:|..|....:.|||
Human   899 QKMLFEERNHF---AEIETELQAELVRMEQQHQEKVLYLLSQLQQSQMAEKQLEESVSEKEQQLL 960

  Fly   910 --------------QICE-------------QELVRFQQSELKTYAPTGT--TP-SKRDFVYPRT 944
                          ::||             |:|...|.:..:.:.|..|  :| |..::|.|:
Human   961 STLKCQDEELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQKHLPKDTLLSPDSSFEYVPPK- 1024

  Fly   945 LVATSPHQEIVRRYRQELDWSDLD--TTATIDECSEGEHDVSMHSVQELSETETIMNSTPIEPVD 1007
               ..|.:...:...|.:|..||.  :..:::|..:|:.|......:|...|:.:..|.  :.:.
Human  1025 ---PKPSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDDDEGDDEEWKPTKLVKVSR--KNIQ 1084

  Fly  1008 GVTVKRGCG 1016
            |.:.|..||
Human  1085 GCSCKGWCG 1093

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	156/354 (44%)
		SMC_prok_B	<408..728	CDD:274008	83/398 (21%)
		SMC_prok_B	<651..>921	CDD:274008	65/353 (18%)
		Microtub_bind	923..>1004	CDD:464048	17/85 (20%)
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	150/346 (43%)
		PRK11281	383..>646	CDD:481316	60/282 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..515		4/18 (22%)
		Smc	<517..>1033	CDD:440809	106/567 (19%)
		Required for the interaction with PRC1. /evidence=ECO:0000269|PubMed:15297875	663..1232		90/469 (19%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11736643	793..798		2/4 (50%)
		Globular	1000..1232		21/100 (21%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269|PubMed:29848660	1086..1144		3/8 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1122..1142