Protein Alignment Klp61F and klp-4

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_001359864.1
• Gene: klp-4 / 180608
• WormBaseID: WBGene00002217
• Length: 1576
• Species: Caenorhabditis elegans
• Alignment Length: 1269
• Identity: 300/1269 - (23%)
• Similarity: 500/1269 - (39%)
• Gaps: 344/1269 - (27%)

  Fly    12 PQKKSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSF---GPE 73
            |.::|  .::|.:||||.|.||..:::..||. :...:.|..|.::.|.:|.||||.||   .|.
 Worm     4 PDEES--AVKVAIRVRPFNKRELDLKTKSVVR-IQKEQCVLHHPIEEKNSKTFTFDHSFCSTDPH 65

  Fly    74 S----KQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGII 134
            |    .|..|...:.|.::|...:|||..:|||||||:||::||:|.           ....|||
 Worm    66 SYDFASQETVSYHLGSGVVENAFSGYNACIFAYGQTGSGKSYTMMGT-----------PDQPGII 119

  Fly   135 PRALSHLF---DELRMMEVEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLE 196
            ||..:.:|   .|.....:.:.:.:||:|:|||.:.|||....::|.....:....|. ::.||.
 Worm   120 PRVCNDIFTRIQETSNSSLSFKVEVSYMEIYNERVRDLLDPKKSSKALKVREHKILGP-MVDGLS 183

  Fly   197 EIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVV----HIRENGIEGEDMLKIGKL 257
            .:.|:|.:.:..|||:|.:.|..|.|.|||:|||||.|||::|    |..|||..||   |:.|:
 Worm   184 ILAVNSFEQISNLLEEGNKSRTVAATNMNAESSRSHAVFSLIVTQTLHDLENGFSGE---KVAKI 245

  Fly   258 NLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVDR----APHVPYRESKLTRLLQ 318
            :||||||||...|.| ..|.|:.|..|||:||.|||.||:||.:|    ...:|||:|.||.||:
 Worm   246 SLVDLAGSERAGKTG-AVGKRLEEGGNINKSLTTLGMVISALAERNSKKDKFIPYRDSVLTWLLK 309

  Fly   319 ESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLK 383
            :||||.::|.:|||:||...:.|||||||.||.|||.|.|...:|:....: |::|..||::.|:
 Worm   310 DSLGGNSRTVMIATLSPAADNYEETLSTLRYADRAKKIVNHAIINEDPNAR-VIRELREEVETLR 373

  Fly   384 RDLMAARDKNGIYLAEETYGEITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEVSMSLVEKT 448
            ..:..                 |.|..::..||.|:          |...|::.::::.|..|:.
 Worm   374 MQITQ-----------------TKKEHAETEELRER----------LAESERLVAQMNKSWEERL 411

  Fly   449 QE---LKKTEENLLNTKGTLLLTKKVLTKTKRRY-------KEKKELVASHMKTEQVLTTQAQEI 503
            :|   |.|..:..|...|..:.:..:..:..|.|       ....||:..::....:: ..::|:
 Worm   412 KETDTLNKERQKDLTEIGISIESSGIKVEKDRFYLVNMNADPSLNELLVYYINGSAII-GNSEEL 475

  Fly   504 LAAADLATDDTHQLHGTIERRRELDEKIR-----RSCDQFKDRMQDNLEMIGGSLNLYQDQQAAL 563
            ..:.|.....|     ..:..|..|:|.|     |.....:...:..:|..||.|.|:
 Worm   476 ETSRDSGLSMT-----CSDSSRRDDDKERTSIVLRGLGIMRRHAKMTVEEYGGRLRLF------- 528

  Fly   564 KEQLSQE---MVNSSYVSQRLALNSSKSI-----EMLKEMC------AQSLQDQTNL--HNKLIG 612
            ...:|.|   .||...:::|..|.:...:     ...|..|      .||:.:.:.:  :|....
 Worm   529 VAPMSSECRICVNGKQITERTLLRNGNRLLVGMNHFFKVNCPKVMDMEQSIMEDSTMFDYNDAWH 593

  Fly   613 EVMKIS------DQHSQAFVAKLMEQ----MQQQQLLMSKEIQ---------------------- 645
            ||...:      ||:.::...|..|.    ::||.....|.||
 Worm   594 EVNDANPISSAVDQYMESVTLKHQEDKKAALEQQYEAFEKYIQSLTAGGFTPSTPMTPGFCLPTP 658

  Fly   646 ----TNL----------QVIEEN----NQRHKAML-DSMQEKFATIIDSS--LQSVEEHAKQMHK 689
                |.|          |.::..    .||.:.|. :|::...|.:|.::  ::.....:|:::|
 Worm   659 ITTPTGLPPFPFPANPKQSVKSKFFYWAQRKEEMFAESLKRLKADVIHANALVREANMISKELNK 723

  Fly   690 KLEQLG----AMSLPDAEELQNLQ---EELANERALAQQEDAL----------LESMMMQMEQIK 737
            |.::..    .:.:| |..|:.::   .:...|..:..:.:.:          |||.::.|....
 Worm   724 KPKRQTTYDVTLQIP-ASNLRPIKIKAGQFVCEPVIVVRREGMSGSQFWTVSQLESRLVDMRDTY 787

  Fly   738 N------LRSKNSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEA 796
            |      .|:..|::.:.|.:.|:.:.:..|                                |.
 Worm   788 NDMLNGFTRTSESLNGTPHASPMKIAGIPMN--------------------------------EC 820

  Fly   797 GMLCLDQ-----------GVANCSMLQVHMKNLNQKYEKETNENVGSV--RVHHNQVEIICQESK 848
            ..|.:|.           ||||. .|:|...:|...|:.......|.|  |:|.....::.||..
 Worm   821 SSLVIDPFFESQEHHNLVGVANV-FLEVLFHDLRLDYQVPIISQQGEVAGRLHVQIFRVVTQEEM 884

  Fly   849 QQLEAVQEKTEVNLEQMVDARQQL-----ITEDRQRFI----------------------GHATV 886
            .  |......|..|.:.:..|.::     :.|....|:                      .|::.
 Worm   885 D--ETSNNGPETLLGKTITCRVRIKRASGLPEKLSNFVFCQYSFFNISELLVVAPANEAANHSSC 947

  Fly   887 ATDLVQESNRQFS-----EHAEHQRQQL--------QICEQELVRFQQSELKTYAPTGTTPSKRD 938
            .|.::.|..|.|:     |..|:.|...        :||.....|...::.|:     .:...|.
 Worm   948 PTTVIFEHQRDFNVMVTEEFMEYVRDDALSIEVWGHRICGHPEERILDTDEKS-----KSLQNRW 1007

  Fly   939 FVYPRTLVATSPHQEIVRRYRQELDWSDLD-------TTATIDECSEGEH--------------- 981
            ....|.|...|.    ||......||:.::       :|..|.:..:|:.
 Worm  1008 MEVTRRLETWSE----VRELNDNGDWTSVEVRHADDVSTGGIYQLKQGQQRRLVVGVNVAAPDGL 1068

  Fly   982 --------DVSMHSVQELSETETI--MNSTPIEPVDGVTVKRGCGTTRNSNSNALKPP------- 1029
                    .||:.::..:..|..:  ::|...|.:|.:         |...|:|||..
 Worm  1069 PISIDCITSVSIGAIMAVKSTNNLKSIDSYQEEDLDKI---------RKQWSHALKSRQYYLQHQ 1124

  Fly  1030 ----VATGGKRSSSLSR---------SLTPSKTS 1050
                .|..||..:.|.|         :||..:|:
 Worm  1125 LDSLSAKSGKSEAELDREHSLMGQWVALTEERTA 1158

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	149/365 (41%)
		SMC_prok_B	<408..728	CDD:274008	69/420 (16%)
		SMC_prok_B	<651..>921	CDD:274008	57/352 (16%)
		Microtub_bind	923..>1004	CDD:464048	16/112 (14%)
klp-4	NP_001359864.1	KISc_KIF1A_KIF1B	10..354	CDD:276816	148/360 (41%)
		Kinesin_assoc	351..463	CDD:465047	24/139 (17%)
		FHA_KIF13	443..570	CDD:438758	24/139 (17%)
		KIF1B	776..840	CDD:463574	13/95 (14%)
		DUF3694	<1239..1293	CDD:463599