Protein Alignment Klp61F and Kif5a

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_032473.2
• Gene: Kif5a / 16572
• MGIID: 109564
• Length: 1027
• Species: Mus musculus
• Alignment Length: 1138
• Identity: 290/1138 - (25%)
• Similarity: 486/1138 - (42%)
• Gaps: 280/1138 - (24%)

  Fly    19 NIQVYVRVRPLNSRERCIRSAEVVDVV-GPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSV 82
            :|:|..|.||||..| .:|..:.:.:. |...|:..       .|.:.|||.|.|.:.|..||..
Mouse     9 SIKVLCRFRPLNQAE-ILRGDKFIPIFQGDDSVIIG-------GKPYVFDRVFPPNTTQEQVYHA 65

  Fly    83 VVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRM 147
            ....::::||.|||.|:||||||.:||||||.|        ...|...:|||||....:|:.:..
Mouse    66 CAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEG--------KLHDPQLMGIIPRIARDIFNHIYS 122

  Fly   148 ME--VEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLL 210
            |:  :|:.:::||.|:|.:::.|||   |.||..:.....|.....::|..|..|.|.:::..::
Mouse   123 MDENLEFHIKVSYFEIYLDKIRDLL---DVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVI 184

  Fly   211 EKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKAGNEK 275
            ::||..|..|.|.||..|||||::|  :::|::..:|.|..|. |||.||||||||.|||.|.|
Mouse   185 DEGKSNRHVAVTNMNEHSSRSHSIF--LINIKQENVETEQKLS-GKLYLVDLAGSEKVSKTGAE- 245

  Fly   276 GIRVRETVNINQSLLTLGRVITALVD-RAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKD 339
            |..:.|..|||:||..||.||:||.: ...:||||:||:||:||:||||..:|::....||...:
Mouse   246 GAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYN 310

  Fly   340 IEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEE----------IDKLKRDL-------- 386
            ..||.|||.:..|||.|:|...||.:||.:...|:|.:|          |.||:.:|
Mouse   311 DAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGEN 375

  Fly   387 ------MAARDKN-GIYLAEET----YGEITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEV 440
                  :|..|.. |..|.|||    ...|.:::..:.|:..|:.  ::.|..:|.:|:...::.
Mouse   376 VPETERLAGEDSALGAELCEETPVNDNSSIVVRIAPEERQKYEEE--IRRLYKQLDDKDDEINQQ 438

  Fly   441 SMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTEQVLTTQAQEILA 505
            |..:.:..|::...||.|::|:|.               .||.:...||:::|.
Mouse   439 SQLIEKLKQQMLDQEELLVSTRGD---------------NEKVQRELSHLQSEN----------- 477

  Fly   506 AADLATDDTHQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQE 570
              |.|.|:..::...:|   ||.....:...:.:::.|.|               ..|.::|||:
Mouse   478 --DAAKDEVKEVLQALE---ELAVNYDQKSQEVEEKSQQN---------------QLLVDELSQK 522

  Fly   571 MVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQ 635
                  |:..|:|.|.          .|.||:.:....|.|.||:.           .||..:.:
Mouse   523 ------VATMLSLESE----------LQRLQEVSGHQRKRIAEVLN-----------GLMRDLSE 560

  Fly   636 QQLLMSK-EIQTNLQV---IEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQM-------HK 689
            ..:::.. ||:..:::   |||.        .::...:.:.|.|.::||.:..:|:       |:
Mouse   561 FSVIVGNGEIKLPVEISGAIEEE--------FTVARLYISKIKSEVKSVVKRCRQLENLQVECHR 617

  Fly   690 KLEQLGAMSLPDAEELQNLQEELANERALAQQEDALLESM--MMQMEQIKNLRSKNSI-SMSVHL 751
            |:|..|              .||::.:.|..|.:|.:.|:  .||..::|....:.|. |:|..|
Mouse   618 KMEVTG--------------RELSSCQLLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSDEL 668

  Fly   752 NKMEESRLTRNHRIDDIKSGIQDYQ------KLGIEASQSAQ----AELTSQMEAGMLCLDQGVA 806
            .:::.........:.|.:...||.:      :|.:|..:.|.    |.|..::......:|:...
Mouse   669 ARLQAHETVHEVALKDKEPDTQDAEEVKKALELQMENHREAHHRQLARLRDEINEKQKTIDELKD 733

  Fly   807 NCSMLQVHMKNLNQKYEKETN-ENVGSVRV--------HHNQVEIICQESKQQLEAVQEKTEVNL 862
            ....||:.::.|...||:..| ||..|.::        .|.|       |||.|:        .|
Mouse   734 LNQKLQLELEKLQADYERLKNEENEKSAKLQELTFLYERHEQ-------SKQDLK--------GL 783

  Fly   863 EQMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTY 927
            |:.|....|.:...|:.|:...|.......|...:.|.....|:|::...|..|.:.
Mouse   784 EETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQL-------- 840

  Fly   928 APTGTTPSKRDFVYPRTLVATSPHQEIVRRYRQELDWSDLDTTATIDECSEGEHDVSMHSVQELS 992
                                |..|:::||      |.:||       .|             ||.
Mouse   841 --------------------TKVHKQLVR------DNADL-------RC-------------ELP 859

  Fly   993 ETETIMNSTP--IEPVDGVTVKRGCGTTRNSNS-----NALKPPV--ATGGKR--SSSLSRSLTP 1046
            :.|..:.:|.  ::.::|...:...|..::...     :.:|..|  .:.|||  |:.:::.:.|
Mouse   860 KLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRP 924

  Fly  1047 ------SKTSPRG--SPAFVRH------NKENV 1065
                  |.|:|.|  ||..:.:      |.:|:
Mouse   925 GHYPASSPTNPYGTRSPECISYTNNLFQNYQNL 957

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	138/349 (40%)
		SMC_prok_B	<408..728	CDD:274008	61/330 (18%)
		SMC_prok_B	<651..>921	CDD:274008	62/298 (21%)
		Microtub_bind	923..>1004	CDD:464048	12/82 (15%)
Kif5a	NP_032473.2	KISc_KHC_KIF5	7..327	CDD:276820	134/340 (39%)
		Microtubule-binding	174..315		66/144 (46%)
		Necessary for interaction with ZFYVE27. /evidence=ECO:0000269|PubMed:21976701	271..361		34/89 (38%)
		Interaction with BICD2. /evidence=ECO:0000250|UniProtKB:Q12840	353..1027		147/771 (19%)
		PRK03918	<409..920	CDD:235175	126/676 (19%)
		Khc_CBD_cc	832..901	CDD:467880	16/122 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	906..937		8/30 (27%)
		Globular	907..1027		13/51 (25%)