Protein Alignment Klp61F and Kif21b

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_001034561.1
• Gene: Kif21b / 16565
• MGIID: 109234
• Length: 1624
• Species: Mus musculus
• Alignment Length: 1130
• Identity: 296/1130 - (26%)
• Similarity: 495/1130 - (43%)
• Gaps: 277/1130 - (24%)

  Fly    20 IQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSVVV 84
            ::|.||:||..|:|: |....:...|.|.|.......|    |.||:|..|..::.|..:||..|
Mouse     9 VKVAVRIRPQLSKEK-IEGCHICTSVTPGEPQVLLGKD----KAFTYDFVFDLDTWQEQIYSTCV 68

  Fly    85 SPLIEEVLNGYNCTVFAYGQTGTGKTHTM-VGNETAELKSSWEDDSDIGIIPRALSHLF---DEL 145
            |.|||....|||.||.||||||.|||:|| .|.:|.      ..:.:.||||||::|||   ||.
Mouse    69 SKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTV------TSEEEQGIIPRAIAHLFRGIDER 127

  Fly   146 R-------MMEVEYTMRISYLELYNEELCDLL-STDD------TTKIRIFDDSTKKGSVIIQGLE 196
            :       :...|:.:...:|||||||:.||. ||.|      .:.|:|.:|:  .|.:...|:.
Mouse   128 KRRAQEKGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDA--NGGIYTTGVT 190

  Fly   197 EIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRE------------------ 243
            ...::|::::.:.|::|...|.||:|.||.||||||.:|:|  |:.:
Mouse   191 SRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTI--HLCQMRVCAQPDLVNETVTGLP 253

  Fly   244 -----NGIEGEDMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVDRA 303
                 .|.|.|.:  ..|.:.|||||||.:.:.| ..|.|.:|.::||..||.||.||:||.|::
Mouse   254 DGAAPTGTEYETL--TAKFHFVDLAGSERLKRTG-ATGERAKEGISINCGLLALGNVISALGDQS 315

  Fly   304 P---HVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQK 365
            .   |||||:||||||||:||||.::|.:||.:||..:|..|||:||:||:||:||:||..|||.
Mouse   316 KKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQD 380

  Fly   366 LTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETYGEITL-----KLESQNRELNEKMLLLKA 425
            .|.:.: .....||.:|:.:||..:....:...:.|.|...|     .|:.:|..|..::..::.
Mouse   381 KTSQQI-SALRAEIARLQMELMEYKAGKRVIGEDGTEGYSDLFRENAMLQKENGALRLRVKAMQE 444

  Fly   426 LKDELQNK-EKIFSEVSMSLVEKTQE------------LKKTEENLLNTKGTLLLTKKVLTKTKR 477
            ..|.:.|: .::.|:.:..|:.|..:            :::.||  |.||   ||..:.:.::.|
Mouse   445 AIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEE--LRTK---LLESEAMNESLR 504

  Fly   478 RYKEKKEL------------------VASHMKTEQVLTTQAQEILAAADLATDDTHQL-HGTIER 523
            |...:...                  .|:.|:       .|.|::..   |..|..:| ...:.:
Mouse   505 RSLSRASARNPYSLGASPAGPAFGGSPATSME-------DASEVIRK---AKQDLERLKKKEVRQ 559

  Fly   524 RRELDEKIRRSCDQFKDRMQ---DNLEMIGGSLNLYQDQ---QAALKEQLSQEMVNSSYVSQRLA 582
            ||:..||     :.||.|.:   :|.|....:....:::   ::..:|:..:|..:....|:...
Mouse   560 RRKSPEK-----EAFKKRAKLQAENSEETDENEAEEEEEERDESGCEEEEGREDEDEDSGSEESL 619

  Fly   583 LNSSKSIEMLKEMCAQS-LQDQT---NLHNKLIGEVMKISDQHSQAFVAKLMEQMQQQQLLMSKE 643
            ::|....|. ||:..|: |.|.|   .:..|||.|:     ::||..:..|..|.:::.:|:..:
Mouse   620 VDSDSDPEE-KEVNFQADLADLTCEIEIKQKLIDEL-----ENSQRRLQTLKHQYEEKLILLQNK 678

  Fly   644 IQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSLPDA------ 702
            |: :.|:..:...::.:.::...|:.|..|.:   ..|:..::|::.|::|.|.....|
Mouse   679 IR-DTQLERDRVLQNLSTMECYTEEKANKIKA---DYEKRLREMNRDLQKLQAAQKEHARLLKNQ 739

  Fly   703 ----EELQNLQEELANERALAQQEDALLESMMMQMEQIKNLRSKNSISMSVHLNKMEESRLTRNH 763
                .||:.||.|:|.   :.:.:.||::.|..:.::.:.:.:|                  ||.
Mouse   740 SRYERELKKLQAEVAE---MKKAKVALMKQMREEQQRRRLVETK------------------RNR 783

  Fly   764 RIDDIK--SGIQDYQKLGIEASQSAQAELT------------------SQMEAGMLCL-----DQ 803
            .|..:|  ...|::|...:| ||..|.|:.                  |:..||.:.|     |.
Mouse   784 EIAQLKKEQRRQEFQIRALE-SQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDS 847

  Fly   804 GVANCSMLQVHMKNLNQKYEKETNENVGSVRVHHNQVEIICQE---------------------- 846
            |.      :|.....:.:.|.........||..:.:::....:
Mouse   848 GA------EVSASTTSSEAESGARSVSSIVRQWNRKIDHFLGDRPTATVNGGRPARKKFQKKGAS 906

  Fly   847 ---SK------QQLE------AVQEKTEVNL----EQMVDARQQLITEDRQRFIGHATVATDLVQ 892
               ||      |.||      .:|..|.|||    |:::..|::|.                |:|
Mouse   907 QSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELF----------------LLQ 955

  Fly   893 ESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIVR- 956
            |:.|:..||.:.:..:.:...|||.    .|::..|      :..|::...   .|.....||:
Mouse   956 EALRRKREHLQAESPEEEKGLQELA----EEIEVLA------ANIDYINDS---ITDCQATIVQL 1007

  Fly   957 -RYRQELDWSDLDTTATIDECSEGE 980
             ..::|||  ..||:..|..||..|
Mouse  1008 EETKEELD--STDTSVVISSCSLAE 1030

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	154/388 (40%)
		SMC_prok_B	<408..728	CDD:274008	72/371 (19%)
		SMC_prok_B	<651..>921	CDD:274008	62/345 (18%)
		Microtub_bind	923..>1004	CDD:464048	15/60 (25%)
Kif21b	NP_001034561.1	KISc_KIF4	7..372	CDD:276823	149/380 (39%)
		SMC_N	367..>1095	CDD:481474	150/754 (20%)
		Interaction with TRIM3. /evidence=ECO:0000269|PubMed:24086586	401..1100		136/719 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	553..629		14/81 (17%)
		EnvC	634..>831	CDD:443969	45/227 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	837..866		5/34 (15%)
		Rcc_KIF21B	930..1011	CDD:410203	23/109 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1199..1253
		WD40	1302..1615	CDD:238121
		WD 1	1308..1345
		WD40 repeat	1314..1348	CDD:293791
		WD 2	1348..1386
		WD40 repeat	1353..1389	CDD:293791
		WD40 repeat	1409..1452	CDD:293791
		WD 3	1412..1450
		WD 4	1453..1495
		WD40 repeat	1458..1502	CDD:293791
		WD 5	1504..1541
		WD40 repeat	1511..1543	CDD:293791
		WD 6	1545..1584
		WD40 repeat	1550..1574	CDD:293791