Protein Alignment Klp61F and Kif16b

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_036014693.1
• Gene: Kif16b / 16558
• MGIID: 1098240
• Length: 1908
• Species: Mus musculus
• Alignment Length: 1209
• Identity: 304/1209 - (25%)
• Similarity: 515/1209 - (42%)
• Gaps: 297/1209 - (24%)

  Fly    29 LNSRERCIRSAEVVDVVGPREVVTR------HTLDS--KLTKKFTFDRSF-GPESKQCD------ 78
            |.|.|:.:.:..::.:...:..:|.      .|.||  :.||.||:|.|| ..::|..|
Mouse    47 LFSGEKDLEAKFIIQMEKSKTTITNLKIPEGGTGDSGRERTKTFTYDFSFYSADTKSPDYVSQEM 111

  Fly    79 VYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFD 143
            |:..:.:.:::....|||..||||||||:||::||:||           ..|.|:|||....||.
Mouse   112 VFKTLGTDVVKSAFEGYNACVFAYGQTGSGKSYTMMGN-----------SGDSGLIPRICEALFS 165

  Fly   144 EL----RMMEVEYTMRISYLELYNEELCDLL--STDDTTKIRIFDDSTKKGSVIIQGLEEIPVHS 202
            .:    |..|..:...:||||:|||.:.|||  .:..|..:|: .:..|:|. .::.|.:..|.:
Mouse   166 RINETTRWDEASFRTEVSYLEIYNERVRDLLRRKSSKTFNLRV-REHPKEGP-YVEDLSKHLVQN 228

  Fly   203 KDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLK-IGKLNLVDLAGSE 266
            ..||.:|::.|...|.||.|.||..|||||.:|:|  ...:...:.|...: :.|::||||||||
Mouse   229 YSDVEELMDAGNINRTTAATGMNDVSSRSHAIFTI--KFTQAKFDAEMPCETVSKIHLVDLAGSE 291

  Fly   267 NVSKAGNEKGIRVRETVNINQSLLTLGRVITALVD------------RAPHVPYRESKLTRLLQE 319
            .....| ..|:|::|..|||:||:|||.||:||.|            :...||||:|.||.||::
Mouse   292 RADATG-ATGVRLKEGGNINKSLVTLGNVISALADLSQDAANPLVKKKQVFVPYRDSVLTWLLKD 355

  Fly   320 SLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKR 384
            ||||.:||.:||||||...:..||||||.||:|||||.|||.:|:....| :::|...||.:||
Mouse   356 SLGGNSKTIMIATISPADVNYGETLSTLRYANRAKNIINKPTINEDANVK-LIRELRAEIARLK- 418

  Fly   385 DLMAARDKNGIYLAEETYGEITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEVSMSLVEKTQ 449
            .|:|  ..|.|.|.:   ....|.:|   .:|::....::.|..|..||   ::|....|.|:|.
Mouse   419 TLLA--QGNQIALLD---SPTALSME---EKLHQNEARVQELTKEWTNK---WNETQNILKEQTL 472

  Fly   450 ELKK----------------TEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASH---MKTEQV 495
            .|:|                .:::||:|...|...|:..|...|.....::.:..|   :::|..
Mouse   473 ALRKEGIGVVLDSELPHLIGIDDDLLSTGIILYHLKEGQTYVGREDASTEQDIVLHGLDLESEHC 537

  Fly   496 LTTQAQEILAAADL-----------ATDDTHQLHGTI--------------ERRRELDEK----- 530
            :...|...:....|           ..|.|....|.:              :...:|.||
Mouse   538 VFENAGGTVTLIPLRGSQCSVNGVQIVDATQLNQGAVILLGRTNMFRFNHPKEAAKLREKRKSGL 602

  Fly   531 ----------IRRSCD-----------------------QFKDRMQDNLEMIGGSLNLYQDQQAA 562
                      :.:||:                       :|:.:.::.||.:.....|.::.:..
Mouse   603 LSSFSLSMTDLSKSCENLSAVMLYNPGLFPIKGPICLRLEFERQQREELEKLESKRKLIEEMEEK 667

  Fly   563 LK------EQLSQEMV---NSSYVSQRLALNSSKSIE--------MLKEMCAQS-------LQDQ 603
            .|      |::.||:.   ..:.:.||......:|::        .||::.|:.       |::|
Mouse   668 QKSDKAELERMQQEVETRRKETEIVQRQIRKQEESLKRRSFHIENKLKDLLAEKERFEEERLREQ 732

  Fly   604 TNLHN----------KLIGEVMKISD--QHSQAFVAKL---MEQMQQQQLLMSKEIQTNLQVIEE 653
            ..|..          ::..|:.|:.:  .|.||...::   ::::.|:|...|.:::...:.:||
Mouse   733 QGLEQQRRQEEESLFRIREELRKLQELNSHEQAEKVQIFQELDRLHQEQNAQSAKLRLEKRRLEE 797

  Fly   654 NNQRHKAMLDSMQEKFATIIDSS---LQSVEEHAKQMHKKLEQLGAMSLPDAE-----------E 704
            ..:.....:..::|:.....|::   .....:.|::..::||.:....|...|           |
Mouse   798 EEKEQVQRVAHLEEQLRKRQDTAPLLCPGEAQRAQEEKRELESIREALLQAKEMRAGGDHTCRDE 862

  Fly   705 LQNLQE-----------ELAN-ERALAQQEDALLESMMMQMEQIKNLR-------------SKNS 744
            |:..|:           :||: |:.|.||:|.|.:.:..:...:::::             ..|.
Mouse   863 LERAQQYFLEFKRRQLVKLASLEKDLVQQKDLLSKEVQEEKVALEHVKCDAGGDPSFLATDDGNI 927

  Fly   745 ISMSVHLNKME--ESRL-TRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLD---- 802
            :.....|:|::  |:|| :|.|::.|:   :|::....:|..|    .:...:::|:|.||
Mouse   928 LGGPPDLDKIKTAETRLQSREHQLQDL---LQNHLPALLEEKQ----RVLDALDSGVLGLDTTLC 985

  Fly   803 --------------QGVANCSMLQVHMKNLNQKYEKETNENVGSVRVHHNQVEIICQESKQQLEA 853
                          |..||.|.||    .|...:|...|......:|...:.||:..:.|||.||
Mouse   986 QVEKEVGEKEEQIAQYQANASQLQ----QLRATFEFTANVARQEEKVRRKEKEILESQEKQQREA 1046

  Fly   854 VQEKTEVNLEQMVDARQQL------ITEDRQRFIG----------HATVATD---LVQESNRQFS 899
            : |:....|||...|.|:.      |.|.||: :|          .|::.||   |..:..|  .
Mouse  1047 L-EQAVAKLEQRRSALQRCSTLDLEIQEQRQK-LGSLHTSEWSGWQASLETDGEALEMDPAR--L 1107

  Fly   900 EHAEHQRQQLQICEQELVRFQ-------QSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIVRR 957
            ||..||.:| :|||.:.|:..       |:.|.:..|:|........:..|  ::....:|:.||
Mouse  1108 EHEIHQLKQ-KICEVDGVQRPHHGILEGQAVLSSLPPSGGNSHLAPLMDAR--ISAYIEEEVQRR 1169

  Fly   958 YRQELDWSDLDTTATIDECSEGEHDVSMHSVQELSETET 996
            ..        |....|.:.:....|| |.|.:||....|
Mouse  1170 LH--------DLHRAIGDANHTPADV-MKSNEELHNGTT 1199

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	141/369 (38%)
		SMC_prok_B	<408..728	CDD:274008	76/466 (16%)
		SMC_prok_B	<651..>921	CDD:274008	78/355 (22%)
		Microtub_bind	923..>1004	CDD:464048	16/74 (22%)
Kif16b	XP_036014693.1	KISc_KIF1A_KIF1B	51..399	CDD:276816	138/363 (38%)
		Kinesin_assoc	398..510	CDD:465047	30/124 (24%)
		FHA	480..596	CDD:469597	15/115 (13%)
		Smc	<644..>1118	CDD:440809	98/489 (20%)