Protein Alignment Klp61F and CENPE

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: NP_001804.2
• Gene: CENPE / 1062
• HGNCID: 1856
• Length: 2701
• Species: Homo sapiens
• Alignment Length: 1408
• Identity: 325/1408 - (23%)
• Similarity: 548/1408 - (38%)
• Gaps: 492/1408 - (34%)

  Fly    20 IQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSVVV 84
            :.|.||||||||||..:  .|...|....:....:.:|.  :|.|.|||.|.......:||..:.
Human     7 VAVCVRVRPLNSREESL--GETAQVYWKTDNNVIYQVDG--SKSFNFDRVFHGNETTKNVYEEIA 67

  Fly    85 SPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRMM- 148
            :|:|:..:.|||.|:||||||.:|||:||:|:|           ..:|:||||:..:|.:::..
Human    68 APIIDSAIQGYNGTIFAYGQTASGKTYTMMGSE-----------DHLGVIPRAIHDIFQKIKKFP 121

  Fly   149 EVEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKG 213
            :.|:.:|:||:|:|||.:.|||......|..|..:...: :|.:..|.|..|::.:...|.:.||
Human   122 DREFLLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNR-NVYVADLTEEVVYTSEMALKWITKG 185

  Fly   214 KERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEG--EDMLKIGKLNLVDLAGSENVSKAGNEKG 276
            ::.|....|.||.:||||||:|.:::..||.|...  |..:|:..||||||||||..::.| ..|
Human   186 EKSRHYGETKMNQRSSRSHTIFRMILESREKGEPSNCEGSVKVSHLNLVDLAGSERAAQTG-AAG 249

  Fly   277 IRVRETVNINQSLLTLGRVITALVDR--APHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKD 339
            :|::|..|||:||..||:||..|.|.  ...:.||:|||||:||.||||..||.||.||:|  ..
Human   250 VRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITP--VS 312

  Fly   340 IEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETYGE 404
            .:|||:.|::|..||.::|.|.||:..|.:.:||.|.:||..||:.|                .|
Human   313 FDETLTALQFASTAKYMKNTPYVNEVSTDEALLKRYRKEIMDLKKQL----------------EE 361

  Fly   405 ITLKLESQNRE-------LNEKMLLLKALKDELQNKEKIFSEVSMSLVEKTQELK---------- 452
            ::|:..:|..|       |.||.||.|...::::|..::.  |:.|.:...||||
Human   362 VSLETRAQAMEKDQLAQLLEEKDLLQKVQNEKIENLTRML--VTSSSLTLQQELKAKRKRRVTWC 424

  Fly   453 ------------------------KTEE---NLL---------------NTKGTL---------- 465
                                    ||.:   |||               ||..||
Human   425 LGKINKMKNSNYADQFNIPTNITTKTHKLSINLLREIDESVCSESDVFSNTLDTLSEIEWNPATK 489

  Fly   466 ---------------------------LLTKKVLTKTKRRYKEKKEL---VASHMKTEQVLTTQ- 499
                                       |.|:|  .:.:.:.|||.:|   .|...||::....|
Human   490 LLNQENIESELNSLRADYDNLVLDYEQLRTEK--EEMELKLKEKNDLDEFEALERKTKKDQEMQL 552

  Fly   500 ------AQEILAAADLATDD-THQLHGTIERRRELDEKIRR-------------------SCDQF 538
                  .:.::..|::...| .::|...:|..||.:::|::                   |.:..
Human   553 IHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQEYIDSQKLENIKMDLSYSLESI 617

  Fly   539 KDRMQ------------------------DNLEM-------------IGGSLNLYQDQQAA---- 562
            :|..|                        :|||:             :...:.|||.|..|
Human   618 EDPKQMKQTLFDAETVALDAKRESAFLRSENLELKEKMKELATTYKQMENDIQLYQSQLEAKKKM 682

  Fly   563 ------------------------------------------LKEQLSQEM-VNSSYVSQRLALN 584
                                                      |:::|::|: .|.:...:.:.|:
Human   683 QVDLEKELQSAFNEITKLTSLIDGKVPKDLLCNLELEGKITDLQKELNKEVEENEALREEVILLS 747

  Fly   585 SSKSIEMLKEMCAQSLQDQT-NLH------NKLIGEVM--------------------------- 615
            ..||:....|...:.:||:: .||      :||..||:
Human   748 ELKSLPSEVERLRKEIQDKSEELHIITSEKDKLFSEVVHKESRVQGLLEEIGKTKDDLATTQSNY 812

  Fly   616 KISDQHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSV 680
            |.:||..|.|....|:..|:.::::.:..:.|.:::..:.:..|  .||......|.:....|.:
Human   813 KSTDQEFQNFKTLHMDFEQKYKMVLEENERMNQEIVNLSKEAQK--FDSSLGALKTELSYKTQEL 875

  Fly   681 EEHAKQMHKKLEQLGAMSLPDAEELQNLQEELANE----RALAQQEDALLESMMMQMEQIKNLRS 741
            :|..:::.::|           .|::.|:|:|.|.    :.:.:::..:.|.:...:|::|.|..
Human   876 QEKTREVQERL-----------NEMEQLKEQLENRDSTLQTVEREKTLITEKLQQTLEEVKTLTQ 929

  Fly   742 KNSISMSVHLNKMEES-RLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGV 805
            :..     .|.:::|| ::.|    |.:||.|.|...:.|:    .|.:|.:.:|:
Human   930 EKD-----DLKQLQESLQIER----DQLKSDIHDTVNMNID----TQEQLRNALES--------- 972

  Fly   806 ANCSMLQVHMKNLN---QKYEKETN------ENVGSVRVHHNQVEIICQESKQQLEA-------- 853
                 |:.|.:.:|   .|..:|.:      ||.|..:....| :::..:.||.|||
Human   973 -----LKQHQETINTLKSKISEEVSRNLHMEENTGETKDEFQQ-KMVGIDKKQDLEAKNTQTLTA 1031

  Fly   854 -----------------VQEKTEVN--LEQMVDARQQLIT------------EDRQRFIGHATVA 887
                             :|||.|:.  ||.::..::||.|            ::..|.:|.....
Human  1032 DVKDNEIIEQQRKIFSLIQEKNELQQMLESVIAEKEQLKTDLKENIEMTIENQEELRLLGDELKK 1096

  Fly   888 TD--LVQESNRQFSEHAEHQR----------------QQLQICEQELVRFQQ------------- 921
            ..  :.||.|....:..|..|                ||||..:|:|:..|:
Human  1097 QQEIVAQEKNHAIKKEGELSRTCDRLAEVEEKLKEKSQQLQEKQQQLLNVQEEMSEMQKKINEIE 1161

  Fly   922 ---SELKTYAPT------------------------------------GTTPSKRDFV--YPRTL 945
               :|||....|                                    .:..::||.:  |.|.:
Human  1162 NLKNELKNKELTLEHMETERLELAQKLNENYEEVKSITKERKVLKELQKSFETERDHLRGYIREI 1226

  Fly   946 VAT---------------SPHQEIVRRYR--------QELDWSDLDTTATIDECSE-----GEHD 982
            .||               ..|||.:...|        |.::..||:.:.|  :..|     .|..
Human  1227 EATGLQTKEELKIAHIHLKEHQETIDELRRSVSEKTAQIINTQDLEKSHT--KLQEEIPVLHEEQ 1289

  Fly   983 VSMHSVQELSETETIMNSTPIEPVDGVTVKRGCGTTRNSNSNA 1025
            ..:.:|:|:|||:..||...:     :|.:   .||::|.:.|
Human  1290 ELLPNVKEVSETQETMNELEL-----LTEQ---STTKDSTTLA 1324

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	139/349 (40%)
		SMC_prok_B	<408..728	CDD:274008	89/567 (16%)
		SMC_prok_B	<651..>921	CDD:274008	65/340 (19%)
		Microtub_bind	923..>1004	CDD:464048	27/146 (18%)
CENPE	NP_001804.2	KISc_CENP_E	6..329	CDD:276825	135/340 (40%)
		SMC_prok_B	345..1131	CDD:274008	146/846 (17%)
		SMC_prok_B	828..1715	CDD:274008	100/548 (18%)
		SMC_prok_B	1332..2129	CDD:274008
		SMC_N	1730..>2573	CDD:481474
		Kinetochore-binding domain	2126..2476
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2355..2376
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2508..2533
		Globular autoinhibitory domain. /evidence=ECO:0000250	2510..2698
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2588..2701