Protein Alignment Klp61F and kif14

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_004913798.2
• Gene: kif14 / 100493263
• XenbaseID: XB-GENE-981231
• Length: 1609
• Species: Xenopus tropicalis
• Alignment Length: 1137
• Identity: 309/1137 - (27%)
• Similarity: 509/1137 - (44%)
• Gaps: 220/1137 - (19%)

  Fly     4 SGGNTSRQPQKKSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDR 68
            |..|...:..|..|..:.|.|||||.:.||...::.:||.:.| :|...:|. |||....|.:|.
 Frog   331 SNFNLEEEHFKAENCAVTVAVRVRPFSKREVNEKAVQVVFMNG-QETTVQHP-DSKQNYIFCYDF 393

  Fly    69 SFGPESK-------QCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWE 126
            ||....|       |..||..:..||:|..|.|||..:|||||||:||::||:|
 Frog   394 SFWSFEKSQPSFVGQASVYEKLAVPLLEWSLLGYNTCLFAYGQTGSGKSYTMMG----------- 447

  Fly   127 DDSDIGIIPRALSHLFDELR---MMEVEYTMRISYLELYNEELCDLLSTDDTTKIRIF--DDSTK 186
            .|.:.|||||..|.||..::   ..::.:.:.|||.|:|||::.|||         ||  ::..|
 Frog   448 FDGEPGIIPRFCSDLFSRIQATMKQKLSFHLEISYFEVYNEKIHDLL---------IFKAENGQK 503

  Fly   187 KGSV----------IIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVV-H 240
            |..:          .::.|....|.|..|:...||.|.::|.||.|.||.:|||||:||::|: .
 Frog   504 KQPLKVREHPLTGPYVEDLSMNVVSSFQDIQSWLELGNKQRATAATGMNDKSSRSHSVFTLVMTQ 568

  Fly   241 IRENGIEGE--DMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVD-- 301
            .:...::|:  |.....::|||||||||..|.| ...|.|::|.|:||:||||||:||:||.:
 Frog   569 TKTEYVDGQEHDHRITSRINLVDLAGSERCSTA-QTSGERLKEGVSINKSLLTLGKVISALSENS 632

  Fly   302 ---RAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVN 363
               :...:|||||.||.||:|||||.:|||:||||||...::|||||||.||.:|:.|.|..:||
 Frog   633 QTRKKTFIPYRESVLTWLLKESLGGNSKTSMIATISPAATNMEETLSTLRYAKQARLIINVAKVN 697

  Fly   364 QKLTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETY----GEIT---LKLESQNRELNEKML 421
            :.|..| :::|...||:|:|...::|::|:     .|.|    .|:|   |||..|.||::|
 Frog   698 EDLNAK-IIRELKAEIEKMKASQLSAKNKD-----SEKYRQCHQEVTSLKLKLCQQEREMSE--- 753

  Fly   422 LLKALKDELQNKEKIFSEVSMSLVEK---TQELKKTEENLLNTKGTLLLTKKVLTKTKR------ 477
            |.:|.||:|:..|: ..:|.|..::|   |.::.....||:|......|::.:|...|.
 Frog   754 LRRAWKDKLEQAEQ-RKKVEMQELQKAGITFKMDNRLPNLVNLNEDPQLSEMLLYMIKEGQTTVG 817

  Fly   478 RYKEKK----ELVASHMKTEQVLTTQAQEILAAADLATDDTH----QLHG-TIERRREL-----D 528
            :||...    :|..:.:..:..:.|..:.|:.........|:    |:.| ||....:.     |
 Frog   818 KYKAGSSHDIQLSGALIAEDHCIITNNENIVCIKPFGDAKTYVNGNQISGSTILHHGDRLILGGD 882

  Fly   529 EKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQEMVNSSYVSQRLALNSS----KSI 589
            ...|.:.....:|:....::..|.    :|.:.|..|.||.:....:...:...|.:.    :.|
 Frog   883 HYFRVNHPMEVNRLNATTQVSDGP----KDYEFAKNELLSAQRSQLAEEIEEARLRAKEEMMEGI 943

  Fly   590 EMLKEMCAQSLQDQTNLHNKLIGEV---MKISDQH-------SQAFVAKLMEQMQQQQLLMSKEI 644
            ::.|||..|.|..|...:.:.|.::   :|...||       ||....::.| :::.::.:.:|:
 Frog   944 QIAKEMAQQELTSQKFAYEEKIRDLEAQLKKESQHKELQEMNSQRATTRIQE-LEEMKIHLQQEV 1007

  Fly   645 QTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSLPDAEELQNLQ 709
            |.|.:.:|             .|..||     .|::|:|..:..|.|:.|.|.....|:|:|.||
 Frog  1008 QFNKKRLE-------------METLAT-----KQALEDHNVRHAKILQALEAEKQKIAKEVQMLQ 1054

  Fly   710 EEL-ANERALAQQ---EDALLESMMMQMEQIKNLRSKNSISMSVHLNKMEESRLTRNH---RIDD 767
            .|. :.:||:|.|   .:..|..|:.:...|.:...|::: .|.| :..::..|..|.   ::.:
 Frog  1055 RERNSQDRAMAVQPTWNNMKLSVMIQEANSISSKLKKHTV-FSRH-DVTDKENLPTNPVQIQVKN 1117

  Fly   768 IKSGIQDYQKL-GIEASQSAQAEL----------------TSQMEAGM-------------LCLD 802
            :|.||..|..| ..|...:|..:|                ..:.|..:             |..:
 Frog  1118 LKLGIVTYWSLEKFEYKLAAMKDLYENHGSTKDDDVFYDPGDEWEPDLSNTSVSRFSRRKSLMKN 1182

  Fly   803 QGVANC----------SMLQVHMKNLNQKYEK--ETNENVGSVRVHHNQVEIICQESKQQLEAVQ 855
            :.::.|          |:...|...|..|...  ..:.:|.| ....:.:..||::.........
 Frog  1183 RRISGCLYEIRVHPIQSLQSGHASGLMNKSSSFLSCSSSVSS-NPSDSHLPGICKDLIASSLDYL 1246

  Fly   856 EKTEVNLEQMVDARQQLITEDRQRFIGHATV----------ATDLVQESNRQFSEHAEHQRQQLQ 910
            .::.|:...|.|:   :|...::..||..||          :||::..:||:...|:    .||.
 Frog  1247 GRSHVDESNMADS---MIDSVKRACIGVRTVSQAYEQQKEDSTDMLFLANREAQSHS----VQLT 1304

  Fly   911 ICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEI---VRR---YRQ-ELDWSDLD 968
            ...|:||     .|..:.|.         :.|.....|.|...:   :|:   |.| .|.....|
 Frog  1305 SAFQQLV-----VLNKHWPR---------LLPEIQEMTKPADNVSLYIRKLGGYLQLLLQGCTSD 1355

  Fly   969 TTATIDECSEGEHDVSMHSVQELSETETIMNS 1000
            ..:.:.|..:....::...:|.:.:...:.|:
 Frog  1356 IASMVTEARDKSFHIAEQILQNIGQLAALTNT 1387

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	154/377 (41%)
		SMC_prok_B	<408..728	CDD:274008	81/360 (23%)
		SMC_prok_B	<651..>921	CDD:274008	66/328 (20%)
		Microtub_bind	923..>1004	CDD:464048	13/85 (15%)
kif14	XP_004913798.2	KISc_KIF1A_KIF1B	347..697	CDD:276816	151/372 (41%)
		Kinesin_assoc	694..812	CDD:465047	38/127 (30%)
		FHA_KIF14	783..890	CDD:438759	18/106 (17%)
		Smc	<912..>1063	CDD:440809	39/169 (23%)