Protein Alignment Klp61F and kif20b

DIOPT Version :10

• Sequence 1: NP_476818.1
• Gene: Klp61F / 38135
• FlyBaseID: FBgn0004378
• Length: 1066
• Species: Drosophila melanogaster
• Sequence 2: XP_012822228.2
• Gene: kif20b / 100145121
• XenbaseID: XB-GENE-1005036
• Length: 1864
• Species: Xenopus tropicalis
• Alignment Length: 1404
• Identity: 304/1404 - (21%)
• Similarity: 517/1404 - (36%)
• Gaps: 479/1404 - (34%)

  Fly     1 MDISGGNTSRQPQKK----SNQNIQVYVRVRPL-------NSRERCIRSAEVVDVVGPREVVTRH 54
            :.:.|..:...|.:|    |.:::|||:||||.       |..:.||...:...|:    |...|
 Frog    43 LSLGGSVSETNPNQKNSLGSQEHMQVYLRVRPFTAVEIEQNEAQDCISIPDSSSVL----VKPPH 103

  Fly    55 TLDS---------KLTKKFTFDRSFGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKT 110
            ...|         .:.:||||...|||::.|...:...:...:.:.:.|.|..:|.||.|..|||
 Frog   104 NSQSCRLSDKGNGSMAQKFTFTHVFGPDTTQAQFFDGTIKQHVIDFMKGQNRLIFTYGVTNAGKT 168

  Fly   111 HTMVGNETAELKSSWEDDSDIGIIPRALSHLFDEL------------------------------ 145
            .|..|.:           .|.||:||::..||:.:
 Frog   169 FTFQGTK-----------DDAGILPRSMDMLFNSIQGRVYTKMDVKPYRCKDYIRLTKEQVKAEA 222

  Fly   146 --------RMMEVEYTMR----------------------------------------------- 155
                    :|.||:.::|
 Frog   223 ALKNSVLRQMKEVDCSIRSNNSRTCQDTTDFSNDEGFTNSSMLSDMDIHVRQSEEFKLPLDSLAK 287

  Fly   156 ----ISYLELYNE---ELCDLLSTDDTTK---IRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLL 210
                :|:.|:|||   :|.|.:|.|.:.|   :|:..|  .||...::.|:.|.|....:..|:|
 Frog   288 FSVWVSFCEIYNECIYDLLDPISGDKSYKRKTLRLALD--LKGYSFVKDLQWIEVSDATEACKIL 350

  Fly   211 EKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGED---MLKIGKLNLVDLAGSENVSKAG 272
            ..||:.:..|.|.:|:.|||||::|:    :|...||..|   :||:.:|.|.||||||..:|..
 Frog   351 ALGKKFQSIAYTKLNSSSSRSHSIFT----VRLLKIEDSDIPRVLKVSELALCDLAGSERCTKTQ 411

  Fly   273 NEKGIRVRETVNINQSLLTLGRVITALVDRA-----PHVPYRESKLTRLLQESLGGRTKTSIIAT 332
            || |.|::|:.|||.|||.||:.|.||....     .|||:||||||..||....|:.|..:|..
 Frog   412 NE-GERLKESGNINTSLLILGKCINALKTSQHSKAHQHVPFRESKLTHYLQSFFSGKGKVCMIVN 475

  Fly   333 ISPGHKDIEETLSTLEYAHRAKNI------QNKPEVNQKLTKKTV--------------LKEYTE 377
            |.......:|||:.|:::..|:.:      ||.....|:.:.:.|              .|..|.
 Frog   476 ICQSASSYDETLNVLKFSAVAQKVLILESSQNSEAYGQRKSAREVSFIINNADSKVWNSRKRATV 540

  Fly   378 EIDKLKRDLMAARDKNGIYLAEE------TY-----------GEITLKLESQNR----------- 414
            :.|....|:  ..|.|....:||      ||           .|:.:|.::..:
 Frog   541 QWDSRLEDV--PEDGNSEEDSEEEECLDCTYQDEEEDDGVQEDELVIKKDAYQKLLDLVEDLKTK 603

  Fly   415 ---ELNEKMLLLKALKDEL--------QNKEKIFS------------------EVSMSLV---EK 447
               |..:|:|:...:::|:        ..:||.||                  |:...||   .|
 Frog   604 LVNEKKDKLLMELKIREEVAKEFTEHFMQREKDFSEHLEKEKELMEERCDERIEIFQDLVRKCSK 668

  Fly   448 TQELKKTEENLLNT------------------KGTLLLTKKV----------------------- 471
            :::..:.:||..||                  :..|.:.||.
 Frog   669 SEDEGEEDENRDNTADQVEQTDTPIDGLFISMQNDLAVIKKQAMDAQVQIISIPDVPGIISKMEQ 733

  Fly   472 --------LTKTKRRYKEKKELVASHM-----------KTEQVLTTQAQEILAAADLATDDTHQL 517
                    |:||:...|:|...:...:           :||:.|::..|:|    |.......|.
 Frog   734 KLKQISCDLSKTQEELKKKSSQLEIQIAKCSRATIQLEETEKKLSSNKQQI----DQLVSMIEQK 794

  Fly   518 HGTIERRRELDEKIRRSCDQFKDRMQD-NLEMI------GGSLNLYQDQQ--------------- 560
            ...|:|.::|.......|:.::..:.. ..|||      .|.....:|.|
 Frog   795 ESAIDRLKDLVSHWEAKCEDYEKTVNGIKGEMIKVNTSAAGRKRPPEDHQCPEEQPPSKKEFAEV 859

  Fly   561 ------AALKE----------QLSQE-----MVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQT 604
                  ||||:          .:.||     :...:|.:|...||.  |:.:|:|..|:|.:..|
 Frog   860 IDTHRPAALKQTHVNNNSKEPAIQQEVEGLRVETENYKTQIHNLNC--SVSLLEEKLARSEEQVT 922

  Fly   605 NLHNK---LIGEVMKISDQ--HSQAFVAKLMEQMQQQQL------LMSKEIQTNL---------- 648
            .:.:|   ||.|:...:|:  ..:..|..||:::.|.:|      ...:.||..|
 Frog   923 EMKSKNQTLISELQACNDRTFSLEGTVKMLMQELDQNKLNSANKVAQIRTIQNKLDEIGKRVDIQ 987

  Fly   649 -----------QVIEENNQRHKAMLDSMQEKFATIIDSSLQS----------------VEEHAKQ 686
                       .::||.||:: ..||:::|..:..:.|:.:|                ::|.:|:
 Frog   988 GKADEDFSNIHDLVEECNQKN-CQLDNLKESNSAKVSSNRESTFYSAIDGLWKKCQEVLQESSKK 1051

  Fly   687 MH--KKLEQLGAMSLPDAEELQNLQEE---LANERALAQQEDALLESMMMQME-QIKNLRSKNSI 745
            .|  ::|||          :|:.|.::   :.|||          |.:..::. ||..|..|:
 Frog  1052 KHLIQELEQ----------KLETLDKDINTMKNER----------EDLQTKLNAQISTLNEKD-- 1094

  Fly   746 SMSVHLNKM--EESRLTRNHRIDDIKS--GIQDYQ---KLGIEASQSAQAELTSQME--AGMLCL 801
            .:.|||..:  |.:.:..|.|.::.:|  .||:.:   ...::..:..|..|.|..|  ..:.||
 Frog  1095 DLIVHLRNLLSERTDILENQRNEEAESEKAIQELKNQANANVQQIKELQCLLDSYKEKCESLKCL 1159

  Fly   802 -DQGVANCS---MLQVHMKNLNQKYE---------KETNENVGSVRVHHNQVEIICQESKQQLEA 853
             :|..|:.|   .|:.::|....|||         .|.|..:|.     |..|:     :::|.|
 Frog  1160 EEQNKASASDTLQLEQNLKEAQTKYEAYEKDITTLNEENRKLGK-----NITEL-----QEKLNA 1214

  Fly   854 VQEKTEVNLEQMV--DARQ-QLITED-RQRFIGHATVATDL--VQESNRQFSEHAEHQRQQLQIC 912
            . |||..:.|:.|  .|:: :|:.:| .||......:..||  .:|...:..:.....::|:|..
 Frog  1215 A-EKTSKDKEEQVGQSAKEIELLKKDLSQRASELKVLQLDLQRKEEDCTELKDKLMDSKKQIQQV 1278

  Fly   913 EQELVRFQQSELKTYAPTGTTPSKRDFV-----YPRTLVATSPHQEIVRRYRQELDWSDLDTTAT 972
            |:|:             :|....||...     |.:.....|...|:.:|..|:|.....|....
 Frog  1279 EKEV-------------SGMREEKRLLTNKVNEYEKLKNQMSRELEMKQRTIQQLKKESADNEKN 1330

  Fly   973 ID-------ECSEGEHDVSMHSVQELSETETIMNSTPIE 1004
            .|       .|.|.:....:  ::::.||......|.:|
 Frog  1331 GDVMQLYQKACQEAQEKEKI--IEDMKETLIEQEQTQVE 1367

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp61F	NP_476818.1	KISc_BimC_Eg5	17..365	CDD:276815	127/472 (27%)
		SMC_prok_B	<408..728	CDD:274008	91/518 (18%)
		SMC_prok_B	<651..>921	CDD:274008	72/319 (23%)
		Microtub_bind	923..>1004	CDD:464048	16/92 (17%)
kif20b	XP_012822228.2	Motor_domain	64..497	CDD:473979	124/454 (27%)
		RBD_KIF20A-like	617..672	CDD:425378	9/54 (17%)
		CwlO1	728..>808	CDD:443091	15/83 (18%)
		SMC_prok_B	<883..1564	CDD:274008	114/536 (21%)