DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment MED14 and MED14

DIOPT Version :9

Sequence 1:	NP_728534.2	Gene:	MED14 / 38073	FlyBaseID:	FBgn0035145	Length:	1555	Species:	Drosophila melanogaster
Sequence 2:	NP_004220.2	Gene:	MED14 / 9282	HGNCID:	2370	Length:	1454	Species:	Homo sapiens

Alignment Length:	1578	Identity:	600/1578 - (38%)
Similarity:	866/1578 - (54%)	Gaps:	205/1578 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MAPTPLPLEQM--PGVGGGGGGYLP-----------------AAQEGGPRINTMSMSVLIDFIIQ 46
            |||..|...|:  ||.||||.|..|                 ||...|.|::|     ||:|::.
Human     1 MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAAASPGYRLST-----LIEFLLH 60

  Fly    47 RTYHELTVLAELLPRKTDMERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQ 111
            |.|.||.||.:|||||:|:|||:||..:|:|||.||.||.|||||.|:..||:|.:.|.||||:|
Human    61 RAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQ 125

  Fly   112 NMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKRQ 176
            .:|||:|||.||.::|:.||.||||:|.||.|::|||||:|.|||||||::|:|.|.||..||:.
Human   126 AILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQA 190

  Fly   177 TLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLVE 241
            ||.:|||:::|||||..|.||:....:.||||.|.|:.||..:||||||:|.||||||.:::|||
Human   191 TLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVE 255

  Fly   242 DKETGDGKSLVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERL 306
            |||||||::|||.:|:::||||:|:||..:...|.::|||||.||.|||||||::|||.|..||.
Human   256 DKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERW 320

  Fly   307 DDNNITVEEYVPGVKLTVSYWRD--LKSELG----YRLTVQSDPSEIGRPLAVVHVPSLGAKESA 365
            .| .:.||.|..|..|::|.|..  |..:.|    :::|::.|.:::.:||.:.|.|.|.|.:|
Human   321 GD-LVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDS- 383

  Fly   366 EVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDCEFNLQGTPAI--LTVPVLS 428
            ::.:||::.:|||:|:|::.:|:.|:..:|.:||    |.|:..:...|.....|:  |.||:|.
Human   384 KLVERAMKIDHLSIEKLLIDSVHARAHQKLQELK----AILRGFNANENSSIETALPALVVPILE 444

  Fly   429 PCLRAEQIHITIDTHTGMFRCHVPKHLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHRRCDKT 493
            ||..:|.:||.:|.|:|||:..: ..||...:::|:..:|.|..::...:.:|:||:..:||.::
Human   445 PCGNSECLHIFVDLHSGMFQLML-YGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQS 508

  Fly   494 LQHLPATATETLPFLVQPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTFHLGFV 558
            ::|||..::|||.........|....::|:::||.|.|...:||::.|....|.::.|.::
Human   509 IKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYY---- 569

  Fly   559 AYQKDELDVIDDSAMQLVSIVAQPHSDIPKCYTKLMRL---IEFDTFVATHGPGTEVDAEVSPHK 620
                            .:|:.|....|.|.....|.:.   |:...|....|..|..:|     |
Human   570 ----------------FMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNA-----K 613

  Fly   621 RKSNGDLLAPPAKQQKTIFP-AYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANAT 684
            ||.:.|.....:|:.|.... ..|...|||.|||||..:||:.|...||...|||.|:|||.:..
Human   614 RKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGF 678

  Fly   685 SLVLKILALPQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRRVLSISIRSQ 749
            |..:::|.:|.....|..||:.                              |.|.:|..:.|.|
Human   679 SHAIRLLKIPPCKGITEETQKA------------------------------LDRSLLDCTFRLQ 713

  Fly   750 TNKNSQVRIWVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYD---FSKTVEDLLNDWSKIVY 811
            ...|   |.||.|.||.:.||..:..:|||..|.||||||....:   ..|.||..||||:.|..
Human   714 GRNN---RTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIAR 775

  Fly   812 LYTLVYDFAEQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGFRLTF--VGG 874
            ||..|.:||..|.:....|.....|:.|:|..|:|.||..|..|.:|.|:.....|.::.  ||.
Human   776 LYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGP 840

  Fly   875 MSAV-NGHSMMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGI-PRPQVPVLSFC 937
            .|.. |.|:.:..||.:..|:..::.|:.|:|.:|..||::|.|||.:|.||: .|.......|.
Human   841 NSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFS 905

  Fly   938 VLAQSPCLIRLTYQAVYCLELRFRANRLVSIRDGASSRFERN-VVEEFTPIQGLKAFLSKYVDES 1001
            :|.||...|||.::.:||:::..|:..:|:|||||.|.|:.: :||.|.|..|||.||:.:||.:
Human   906 ILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSN 970

  Fly  1002 AVYRGRASHEDDNPLSPMG--MEDNFGGPSSVAGVS-AGGSSPFLGTGMRGPQSPRDSGLRFPAP 1063
            ...|.|:.:|||||.||:|  |.|     |.::.:. .....||       |:.|..|| .:|..
Human   971 QDARRRSVNEDDNPPSPIGGDMMD-----SLISQLQPPPQQQPF-------PKQPGTSG-AYPLT 1022

  Fly  1064 HTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPLNPQPSPHM 1128
             :||:|.  |:..:..||.     ...|..||.:..|.|:..:..|||:..:|:    |.||.|.
Human  1023 -SPPTSY--HSTVNQSPSM-----MHTQSPGNLHAASSPSGALRAPSPASFVPT----PPPSSHG 1075

  Fly  1129 VHSPGPNTLYMQSH----QDSPFTAMSPAN--NNWPGSPSMPRPSP---RPGQSPDHKSTGGGAG 1184
            : |.||...:...|    ..||:|.:||:.  .||||||.:..|||   .||.||.:.|......
Human  1076 I-SIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVP 1139

  Fly  1185 VAGGTDRGGS------------RGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDINVPDM--- 1234
            .|..:.|.|:            |....|.||.::||:|||.||..|..|||.|..   ||.:
Human  1140 DASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGL---VPGLAGS 1201

  Fly  1235 ---SPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMHMQTLHLKV 1296
               ||||||||.|.|||.|.|.|| :|||..:||.||||::||.|.|:|:|.|:....|||.|||
Human  1202 YLCSPLERFLGSVIMRRHLQRIIQ-QETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKV 1265

  Fly  1297 SQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLKDFVQIMRL 1361
            :     |:.   ..|...|:|.|:|::|:||||.||::.|:|.:..:||..|..:|:|.|.||:|
Human  1266 T-----PEN---AGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKL 1322

  Fly  1362 DLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQITR---IPYGAVIGVGK 1423
            :|.|: ...||||.||.||.:||||.||.|.|...||: :.|:|||||:|:   :|.
Human  1323 ELFPD-QATQLKWNVQFCLTIPPSAPPIAPPGTPAVVL-KSKMLFFLQLTQKTSVPP-------- 1377

  Fly  1424 DWKDSPSLVLPIVYDIQTNVTQLA----ERTGQVISPTMTAASTLLRRFAEFN-AQQNQCTLFPA 1483
              ::..|:::||:||:.:..||.|    ::...|.:|.|  .|.:|:||||.| .:|.:||:|.|
Human  1378 --QEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMM--VSNILKRFAEMNPPRQGECTIFAA 1438

  Fly  1484 ITDLLTNLQLAAEMPQPP 1501
            :.||:.||.|      ||
Human  1439 VRDLMANLTL------PP 1450

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
MED14	NP_728534.2	Med14	34..224	CDD:285801	107/189 (57%)
MED14	NP_004220.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..34		17/51 (33%)
		Med14	51..238	CDD:400802	111/186 (60%)
		LXXLL motif 1	69..73		2/3 (67%)
		Interaction with STAT2. /evidence=ECO:0000269\|PubMed:12509459	188..566		146/385 (38%)
		bipartite nuclear localization signal	613..630		5/16 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	973..1167		67/203 (33%)
		Herpes_BLLF1	<1015..>1189	CDD:282904	57/185 (31%)
		LXXLL motif 2	1182..1186		0/3 (0%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165148765
Domainoid	1	1.000	688	1.000	Domainoid score	I136
eggNOG	1	0.900	-	-		E1_KOG1875
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H22082
Inparanoid	1	1.050	914	1.000	Inparanoid score	I363
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG50140
OrthoDB	1	1.010	-	-		D1215335at2759
OrthoFinder	1	1.000	-	-		FOG0005245
OrthoInspector	1	1.000	-	-		oto90666
orthoMCL	1	0.900	-	-		OOG6_104094
Panther	1	1.100	-	-	LDO	PTHR12809
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R704
SonicParanoid	1	1.000	-	-		X5631
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.800