DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment MED14 and med14

DIOPT Version :9

Sequence 1:	NP_728534.2	Gene:	MED14 / 38073	FlyBaseID:	FBgn0035145	Length:	1555	Species:	Drosophila melanogaster
Sequence 2:	NP_997930.2	Gene:	med14 / 336923	ZFINID:	ZDB-GENE-030131-8867	Length:	1422	Species:	Danio rerio

Alignment Length:	1570	Identity:	597/1570 - (38%)
Similarity:	856/1570 - (54%)	Gaps:	231/1570 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MAPTPLPLE-QMPGVGGGGGGYLPAAQEGGPRINTMSMSVLIDFIIQRTYHELTVLAELLPRKTD 64
            |||..:..: |:  |..||....|....|.|....:.:|:||:|::||||||:|:||||||||||
Zfish     1 MAPVQIGSDGQL--VPAGGPTSTPQPPPGAPATQGVRLSLLIEFLLQRTYHEITLLAELLPRKTD 63

  Fly    65 MERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQNMLFVETADMLARMSRET 129
            ||||:||..:|:|||.||.||.|||||.::..||:|.:.|.||||:|..|||:|||.||.::|:.
Zfish    64 MERKIEIVQFASRTRQLFVRLLALVKWASNAGKVEKCAMISSFLDQQAYLFVDTADRLASLARDA 128

  Fly   130 LVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKRQTLLRLNQVIQHRLVTGKL 194
            ||.||||:|.||.|::|||||:|.|||||||::|:|.|.||.|||:.||.:|||:::|||||..|
Zfish   129 LVHARLPSFAIPFAIDVLTTGSYPRLPTCIRDKIIPPDPITKAEKQTTLNQLNQILRHRLVTTDL 193

  Fly   195 LPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLVEDKETGDGKSLVHPLQVNY 259
            .||:....:.||||.|.|:.||..:||||||:|.:|||||.:::||||||||||::|||.:||||
Zfish   194 PPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQVNY 258

  Fly   260 IHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERLDDNNITVEEYVPGVKLTV 324
            ||:|:|:||..:...|.::|.|||.||.|||||||::|||.|..||..| .:.||.|:|...||:
Zfish   259 IHELVQSRLFGDDKPLQDMYTCLHSFCLSLQLEVLHSQTLMLIRERWGD-LVQVERYIPAKCLTL 322

  Fly   325 SYWRD--LKSELG----YRLTVQSDPSEIGRPLAVVHVPSLGAKESAEVADRAVRSEHLSMERLI 383
            |.|..  |..:.|    :::|::.|.|:..:||.:.|.|.|.|.:| ::.:||::.:|||:|:|:
Zfish   323 SVWNQQVLGRKTGTASVHKVTIKIDESDGSKPLQISHEPPLPACDS-KLMERAMKIDHLSVEKLL 386

  Fly   384 VHTVYIRSVSRLSDLKLEFQAFLKDVDCEFN--LQGTPAILTVPVLSPCLRAEQIHITIDTHTGM 446
            :.:|:.||..:|.:||    |.||..:...|  ::.....|.:|:|.||.|:|.:||.:|.|:||
Zfish   387 IDSVHARSHQKLQELK----AILKSSNPNDNSFIETALPTLVIPILEPCGRSECLHIFVDLHSGM 447

  Fly   447 FRCHVPK--HLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHRRCDKTLQHLPATATETLPFLV 509
            |:   |.  .:|..::::::..:|.|..::...|.:|:||:..:||.::::|||...|:||....
Zfish   448 FQ---PMLYGVDQSMLDDIEKSINDDMKRIVTWLLQLKFWLGEQRCRQSVKHLPTLCTDTLHLSN 509

  Fly   510 QPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTFHLGFVAYQKDELDVIDDSAM- 573
            .....:....:|::::||.|.|...:||::.:..:...|::|.::...|:..:.| |.:..:.:
Zfish   510 LTTHPVGNLSKHRLFIKLTRLPQYYIVVEMFDVPSNLTELQYKYYFLSVSQMESE-DGLPSAQLF 573

  Fly   574 -----QLVSIVAQPHSDIPKCYTKLMRLIEFDTFVATHGPGTE-----VDAEVSPHKRKSNGDLL 628
                 .|..:|    .||..|     |::.         |||:     ..:.|.|.|.|.:|::.
Zfish   574 RHFKPNLEELV----QDISSC-----RMVR---------PGTKRKLSGEQSAVEPKKPKRSGEMC 620

  Fly   629 APPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANATSLVLKILAL 693
            |             |..||||:|||||..:|.:.|...||...|||.|:|||.:..|..::||.:
Zfish   621 A-------------FNKELAHLVAMCDTNMPLIGLRSELSNMEIPHQGVQVEGDGCSHAIRILKV 672

  Fly   694 PQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRRVLSISIRSQTNKNSQVRI 758
            |.               ..||..|.:.:               |.|.:|..:.|.|...|   |.
Zfish   673 PP---------------CMAVGEETRKA---------------LERSLLDCTFRLQGRNN---RT 704

  Fly   759 WVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYD---FSKTVEDLLNDWSKIVYLYTLVYDFA 820
            ||.|.:|.|.||.|:..|||.|.|.||||||....:   ..|.||..||||:.|..||..|.:|:
Zfish   705 WVAELIFSSCPLNSTSIKEQASTRHVYLTYENPLSEPVGGRKVVEMFLNDWNSISKLYECVLEFS 769

  Fly   821 EQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGFRLTF--VGGMSAV-NGHS 882
            ..|......|.....::.|:|..|:|.||..|..|..|.|:..:|.:.|..  ||..|.. |.|:
Zfish   770 RSLTEMPSYLSLFSEIRLYNYRKLVLCYGSTKGSSVTIQWNSVTHRYHLALGTVGPNSGCSNCHN 834

  Fly   883 MMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGI-PRPQVPVLSFCVLAQSPCLI 946
            ::..||.:..|:..::.|:.|:|.:|..||::|.|||.:|.||: .|.......|.:|.|||..|
Zfish   835 IILHQLQEMFNKTPNVVQLLQVLSDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSPTHI 899

  Fly   947 RLTYQAVYCLELRFRANRLVSIRDGASSRFER-NVVEEFTPIQGLKAFLSKYVDESAVYRGRASH 1010
            ||.::.:||:::..|:..:|:|||||.|.|:. .:||.|.|..|||.||:.:||.:...|.|:.:
Zfish   900 RLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNTKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVN 964

  Fly  1011 EDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRDSGLRFPAPHTPPSSSNPHTP 1075
            |||||.||:.|||.|                                             .|||.
Zfish   965 EDDNPPSPISMEDTF---------------------------------------------MPHTQ 984

  Fly  1076 ASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPS-------------------GLMPSSPLN 1121
            ..|     .|.|..|.      |||||.|:...||||                   .|...||..
Zfish   985 TQP-----PGRGVYPP------LTSPPNPYHVPPSPSMITTQSPAGNIHAANSPSGALRAPSPFG 1038

  Fly  1122 PQPSPHMVH-SPGPNTLYMQSH----QDSPFT--AMSPANNNWPGSPSMPRPSPR---PGQSPDH 1176
            |.|||..:. |.|..:.:...|    ..||:|  :.||....|||||.:..|||.   ||.||.:
Zfish  1039 PTPSPSSLGISMGQTSNFASPHGTLDPSSPYTLVSASPRVGPWPGSPQVSGPSPGARIPGMSPGN 1103

  Fly  1177 KSTGGGAGVAGGTDRGGS--------RGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDINVPD 1233
            .|..........:.|.|:        |....|.||.::||:|||..|..|..|||.|   ..||.
Zfish  1104 PSLHSPIPDPSHSPRAGTSSQAMPPPRKLPQRSWAASIPTILTHSKLHVLLLPSPMP---CPVPG 1165

  Fly  1234 M------SPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMHMQTL 1292
            :      ||||||||.|.|||.|.|.||.|..||.:||.||||::||.:.|:|:|.||....|||
Zfish  1166 LAGSYLCSPLERFLGSVIMRRHLQRIIQLEPNLTIVNSNEPGVIMFKTEVLKCRVALNPKSYQTL 1230

  Fly  1293 HLKVSQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLKDFVQ 1357
            .|||:     |:...|   .||::|.|:|::|:||||.||::.|:|::..:||..|..:|:|.|:
Zfish  1231 QLKVT-----PENTGP---WSQEELQVLEKFFETRVAGPPFKYNTLNAFTKLLGAPTNILRDCVR 1287

  Fly  1358 IMRLDLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQIT-RIPYGAVIGV 1421
            ||:|:|.|:... ||.|.||.||.:||||.||.|.|...||: :.|:|||||:| |:|..
Zfish  1288 IMKLELFPDQAA-QLNWNVQFCLTIPPSAPPIAPPGTIAVVL-KTKMLFFLQLTQRLPVP----- 1345

  Fly  1422 GKDWKDSPSLVLPIVYDIQTNVTQLAERTGQVISPTMTA--ASTLLRRFAEFN-AQQNQCTLFPA 1483
                ::..|:::|||||:.|.:||.|:...|..|....|  .|::||||.|.: .:..:||:|.|
Zfish  1346 ----QEPISIIVPIVYDMATGLTQQADIPRQHSSSGAAALMVSSILRRFNEHHPPRPGECTIFAA 1406

  Fly  1484 ITDLLTNLQL 1493
            :.:|:.||.|
Zfish  1407 VHELMANLTL 1416

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
MED14	NP_728534.2	Med14	34..224	CDD:285801	113/189 (60%)
med14	NP_997930.2	Med14	36..223	CDD:285801	113/186 (61%)
med14	NP_997930.2	Dsh_C	962..1149	CDD:289114	67/242 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170582679
Domainoid	1	1.000	678	1.000	Domainoid score	I135
eggNOG	1	0.900	-	-		E1_KOG1875
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H22082
Inparanoid	1	1.050	915	1.000	Inparanoid score	I357
OMA	1	1.010	-	-		QHG50140
OrthoDB	1	1.010	-	-		D1215335at2759
OrthoFinder	1	1.000	-	-		FOG0005245
OrthoInspector	1	1.000	-	-		oto41182
orthoMCL	1	0.900	-	-		OOG6_104094
Panther	1	1.100	-	-	LDO	PTHR12809
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R704
SonicParanoid	1	1.000	-	-		X5631
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800