DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment MED14 and Med14

DIOPT Version :9

Sequence 1:	NP_728534.2	Gene:	MED14 / 38073	FlyBaseID:	FBgn0035145	Length:	1555	Species:	Drosophila melanogaster
Sequence 2:	NP_001041673.1	Gene:	Med14 / 26896	MGIID:	1349442	Length:	1459	Species:	Mus musculus

Alignment Length:	1586	Identity:	603/1586 - (38%)
Similarity:	864/1586 - (54%)	Gaps:	216/1586 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MAPTPLPLEQM--PGVGG---GGGGYL-----------PAAQEGGPRINTMS----MSVLIDFII 45
            |||..|...|:  ||.||   ||||..           |||..........|    :|.||:|::
Mouse     1 MAPVQLDNHQLIPPGGGGGSSGGGGSSSGSASAPAPPPPAAAVAAAAAAAASPGYRLSTLIEFLL 65

  Fly    46 QRTYHELTVLAELLPRKTDMERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDK 110
            .|.|.||.||.:|||||:|:|||:||..:|:|||.||.||.|||||.|...||:|.:.|.||||:
Mouse    66 HRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANDAGKVEKCAMISSFLDQ 130

  Fly   111 QNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKR 175
            |.:|||:|||.||.::|:.||.||||:|.||.|::|||||:|.|||||||::|:|.|.||..||:
Mouse   131 QAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQ 195

  Fly   176 QTLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLV 240
            .||.:|||:::|||||..|.||:....:.||||.|.|:.||..:||||||:|.||||||.:::||
Mouse   196 ATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPEVPWRLLKLEILV 260

  Fly   241 EDKETGDGKSLVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYER 305
            ||||||||::|||.:|:::||||:|:||..:...|.::|||||.||.|||||||::|||.|..||
Mouse   261 EDKETGDGRALVHSMQIDFIHQLVQSRLFADEKPLQDMYNCLHCFCLSLQLEVLHSQTLMLIRER 325

  Fly   306 LDDNNITVEEYVPGVKLTVSYWRD--LKSELG----YRLTVQSDPSEIGRPLAVVHVPSLGAKES 364
            ..| .:.||.|..|..|::|.|..  |..:.|    :::|::.|.:::.:||.:.|.|.|.|.:|
Mouse   326 WGD-LVQVERYHAGKSLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDS 389

  Fly   365 AEVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDCEFNLQGTPAI------LT 423
             ::.:||::.:|||:|:|::.:|:.|:..||.:||    |.|:    .||...:.:|      |.
Mouse   390 -KLVERAMKIDHLSIEKLLIDSVHARAHQRLQELK----AILR----SFNANESSSIETALPALI 445

  Fly   424 VPVLSPCLRAEQIHITIDTHTGMFRCHVPKHLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHR 488
            ||:|.||..:|.:||.:|.|:|||:..: ..||...:|:|:..||.|..::...:.:|:||:..:
Mouse   446 VPILEPCGNSECLHIFVDLHSGMFQLML-YGLDPATLEDMEKSLNDDMKRIIPWIQQLKFWLGQQ 509

  Fly   489 RCDKTLQHLPATATETLPFLVQPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTF 553
            ||.::::|||...||||.........|....::|:::||.|.|...:||::.|....|.::.|.:
Mouse   510 RCKQSIKHLPTITTETLQLANYSTHPIGSLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYNY 574

  Fly   554 HLGFVAYQKDE--------LDVIDDSAMQLVSIVAQPHSDIPKCYTKLMRLIEFDTFVATHGPGT 610
            :...|:....|        |....|:...|:|            |||..:    .|...|....:
Mouse   575 YFMSVSTADREDSPVMALLLQQFKDNIQDLMS------------YTKTGK----QTRTGTKHKLS 623

  Fly   611 EVDAEVSPHKRKSNGDLLAPPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHS 675
            :....:...|.|.:|::.|             |...|||.|||||..:||:.|...||...|||.
Mouse   624 DDPCPIDSKKAKRSGEMCA-------------FNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQ 675

  Fly   676 GLQVEANATSLVLKILALPQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRR 740
            |:|||.:..:..:::|.:| |.|              .::.|.:.:               |.|.
Mouse   676 GVQVEGDGFNHAIRLLKIP-PCK--------------GISEETQKA---------------LDRS 710

  Fly   741 VLSISIRSQTNKNSQVRIWVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYD---FSKTVEDL 802
            :|..:.|.|...|   |.||.|.||.:.||..:..:|||..|.||||||....:   ..|.||..
Mouse   711 LLDCTFRLQGRNN---RTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMF 772

  Fly   803 LNDWSKIVYLYTLVYDFAEQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGF 867
            |||||.|..||..|.:||..|......|.....|:.|:|..|:|.||..|..|.:|.|:.....|
Mouse   773 LNDWSSIARLYECVLEFARSLPEIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKF 837

  Fly   868 RLTF--VGGMSAV-NGHSMMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGI-PR 928
            .:..  ||..|.. |.|:.:..||.:..|:..::.|:.|:|.:|..||::|.|||.:|.||: .|
Mouse   838 HIALGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQR 902

  Fly   929 PQVPVLSFCVLAQSPCLIRLTYQAVYCLELRFRANRLVSIRDGASSRFERN-VVEEFTPIQGLKA 992
            .......|.:|.||...|||.::.:||:::..|:..:|:|||||.|.|:.: :||.|.|..|||.
Mouse   903 TNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKT 967

  Fly   993 FLSKYVDESAVYRGRASHEDDNPLSPMG--MEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRD 1055
            ||:.:||.:...|.|:.:|||||.||:|  |.|     |.::.:......||       |:.|..
Mouse   968 FLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMD-----SLISQLQPPQQQPF-------PKQPGT 1020

  Fly  1056 SGLRFPAPHTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPL 1120
            || .:|.. :||:|.  |:..:..||.     ...|..||.:..|.|:..:..|||:..:|:
Mouse  1021 SG-AYPLT-SPPTSY--HSTVNQSPSM-----MHTQSPGNLHAASSPSGALRAPSPASFVPT--- 1073

  Fly  1121 NPQPSPHMVHSPGPNTLYMQSH----QDSPFTAMSPAN--NNWPGSPSMPRPSPR---PGQSP-- 1174
             |.||.|.: |.||...:...|    ..||:|.:||:.  .||||||.:..|||.   ||.||
Mouse  1074 -PPPSSHGI-SIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRLPGMSPAN 1136

  Fly  1175 ----------DHKSTGGGAGVAGGTDRGGSRGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDI 1229
                      .|....|.:.....|:....|....|.||.::||:|||.||..|..|||.|..
Mouse  1137 PSLHSPVPDVSHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGL-- 1199

  Fly  1230 NVPDM------SPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMH 1288
             ||.:      ||||||||.|.|||.|.|.|| :|||..:||.||||::||.|.|:|:|.|:...
Mouse  1200 -VPGLAGSYLCSPLERFLGSVIMRRHLQRIIQ-QETLQLINSNEPGVIMFKTDALKCRVALSPKT 1262

  Fly  1289 MQTLHLKVSQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLK 1353
            .|||.|||:     |:.   ..|...|:|.|:|::|:||||.||::.|:|.:..:||..|..:|:
Mouse  1263 NQTLQLKVT-----PEN---AGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILR 1319

  Fly  1354 DFVQIMRLDLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQITR---IPY 1415
            |.|.||:|:|.|: ...||||.||.||.:||||.||.|.|...||: :.|:|||||:|:   :|.
Mouse  1320 DCVHIMKLELFPD-QATQLKWNVQFCLTIPPSAPPIAPPGTPAVVL-KSKMLFFLQLTQKTSVPP 1382

  Fly  1416 GAVIGVGKDWKDSPSLVLPIVYDIQTNVTQLA----ERTGQVISPTMTAASTLLRRFAEFN-AQQ 1475
                      ::..|:::||:||:.:..||.|    ::...|.:|.|  .|.:|:||||.| .:|
Mouse  1383 ----------QEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMM--VSNILKRFAEMNPPRQ 1435

  Fly  1476 NQCTLFPAITDLLTNLQLAAEMPQPP 1501
            .:||:|.|:.||:.||.|      ||
Mouse  1436 GECTIFAAVRDLMANLTL------PP 1455

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
MED14	NP_728534.2	Med14	34..224	CDD:285801	108/193 (56%)
Med14	NP_001041673.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..37		13/35 (37%)
		Med14	57..244	CDD:285801	107/186 (58%)
		LXXLL motif 1	75..79		1/3 (33%)
		Interaction with STAT2. /evidence=ECO:0000250	194..572		157/388 (40%)
		Interaction with SREBF1. /evidence=ECO:0000250	506..830		111/385 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	979..1171		65/217 (30%)
		Jun	<1052..1147	CDD:281890	32/99 (32%)
		LXXLL motif 2	1187..1191		1/3 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838849
Domainoid	1	1.000	694	1.000	Domainoid score	I130
eggNOG	1	0.900	-	-		E1_KOG1875
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H22082
Inparanoid	1	1.050	918	1.000	Inparanoid score	I359
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG50140
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0005245
OrthoInspector	1	1.000	-	-		oto94255
orthoMCL	1	0.900	-	-		OOG6_104094
Panther	1	1.100	-	-	LDO	PTHR12809
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R704
SonicParanoid	1	1.000	-	-		X5631
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.790