DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment trh and Hif1a

DIOPT Version :9

Sequence 1:	NP_001261207.1	Gene:	trh / 38065	FlyBaseID:	FBgn0262139	Length:	1022	Species:	Drosophila melanogaster
Sequence 2:	NP_001300848.1	Gene:	Hif1a / 15251	MGIID:	106918	Length:	837	Species:	Mus musculus

Alignment Length:	934	Identity:	238/934 - (25%)
Similarity:	375/934 - (40%)	Gaps:	268/934 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    85 ELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPW 149
            |.|||||||||||||.||:..|||||..||||..::|.|||||::|||||||::|.....|.
Mouse    17 ERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGG--- 78

  Fly   150 TREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQV 214
               ..|..::|:       .:|.|       .|::||||.:.:..||..:|||:.|:.|:||:|.
Mouse    79 ---LDSEDEMKA-------QMDCF-------YLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQF 126

  Fly   215 EMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGT 279
            |:||.|:||:.|..||.|:.:.|                                         |
Mouse   127 ELTGHSVFDFTHPCDHEEMREML-----------------------------------------T 150

  Fly   280 HGTNNPDVAASMTQASTSGYKGYDRSFCVRMKSTLTKRG--CHFKSSGYRASDATSNCNNGNNAS 342
            | .|.|  .....:.:|      .|||.:|||.|||.||  .:.||:.::....|.:.:..:..|
Mouse   151 H-RNGP--VRKGKELNT------QRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNS 206

  Fly   343 NNAKNVKNPGSNYSVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVT 407
            |                     :||..:     .:||:..:|.:...:|.||..||.|:...|::
Mouse   207 N---------------------QPQCGY-----KKPPMTCLVLICEPIPHPSNIEIPLDSKTFLS 245

  Fly   408 RVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMN 472
            |.:.|::.::|:.|:::|:.|.||:|:.:|:|...||.|::.|.|:|.|:..||||.||.||::.
Mouse   246 RHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLA 310

  Fly   473 KSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDK 537
            |.|||.|::|.|||:.:|||:..|.|:|||||:|.....::|....|.|
Mouse   311 KRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTE---------------- 359

  Fly   538 SSGSPGGDASGEGNSHLSAGDMKLNS--PKTDSEGHS--------HRGRGRSAAASHGSSMNSLT 592
            |...|           :.:.|||:..  .|.:||..|        ........|.:.|.::.||.
Mouse   360 SVLKP-----------VESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLD 413

  Fly   593 MIKDSPTPLGVEIDSGVLPTTVATP-------VPAATPPVQSTKRKRKTKASQHAEDQGQEQVIS 650
            ...|.......:::...|...|..|       :..|..|:.|::..:..::|       .:..::
Mouse   414 FGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSS-------ADPALN 471

  Fly   651 EQPLPKLPTMEQRDQQPRS-----RLPSIVDEQPSSAADSAVKDL--EQAMSKHLPSPAAVVSVA 708
            ::...||      :..|.|     .:|.|.| ||:|.:|.:.:..  |:.:.:::.:|    :.:
Mouse   472 QEVALKL------ESSPESLGLSFTMPQIQD-QPASPSDGSTRQSSPERLLQENVNTP----NFS 525

  Fly   709 PPNTD-----------------------FSADSLLK-------------------------QQQQ 725
            .||:.                       |:.|:..|                         |.:.
Mouse   526 QPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRS 590

  Fly   726 QQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQ 790
            ..||.|.|.:|           |..|..:|....|....::..|.|||..|.|        .|:.
Mouse   591 FDQLSPLESNS-----------PSPPSMSTVTGFQQTQLQKPTITATATTTAT--------TDES 636

  Fly   791 TGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSS 855
            .        :|:....:.:::..|:    |...:...:..|...|.|.|.||      ....|..
Mouse   637 K--------TETKDNKEDIKILIAS----PSSTQVPQETTTAKASAYSGTHS------RTASPDR 683

  Fly   856 VSPRDSNQPGKAAP----VLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSG 916
            ...|...|..||.|    :.|:....:..|:.          ::.|.|: ....|.....|...|
Mouse   684 AGKRVIEQTDKAHPRSLNLSATLNQRNTVPEE----------ELNPKTI-ASQNAQRKRKMEHDG 737

  Fly   917 STTTEGGVTYSNLDQPQYFAPHSS 940
            |.....|:. :.|.||...||..|
Mouse   738 SLFQAAGIG-TLLQQPGDCAPTMS 760

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trh	NP_001261207.1	HLH	85..137	CDD:238036	37/51 (73%)
		PAS	176..236	CDD:214512	25/59 (42%)
		PAS	180..>236	CDD:279347	25/55 (45%)
		PAS	403..505	CDD:238075	45/101 (45%)
		PAS_3	415..502	CDD:285623	39/86 (45%)
Hif1a	NP_001300848.1	HLH	16..69	CDD:238036	37/51 (73%)
		DNA-binding. /evidence=ECO:0000269\|PubMed:26245371	22..31		8/8 (100%)
		PAS	94..149	CDD:214512	25/54 (46%)
		Required for heterodimer formation with ARNT. /evidence=ECO:0000269\|PubMed:26245371	171..192		10/20 (50%)
		PAS	240..339	CDD:238075	41/98 (42%)
		PAS_3	255..340	CDD:285623	39/84 (46%)
		N-terminal VHL recognition site	381..418		7/36 (19%)
		ODD	402..614		39/240 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	493..512		6/19 (32%)
		NTAD	545..589		4/43 (9%)
		HIF-1	565..594	CDD:288296	1/28 (4%)
		C-terminal VHL recognition site	570..586		0/15 (0%)
		ID	590..796		46/220 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	594..685		25/127 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	708..735		5/37 (14%)
		Nuclear localization signal. /evidence=ECO:0000255	729..732		0/2 (0%)
		CTAD	797..837
		HIF-1a_CTAD	800..836	CDD:285931
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167847534
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3558
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.740