DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF3 and CDC24

DIOPT Version :9

Sequence 1:	NP_612024.4	Gene:	RhoGEF3 / 38050	FlyBaseID:	FBgn0264707	Length:	3519	Species:	Drosophila melanogaster
Sequence 2:	NP_009359.1	Gene:	CDC24 / 851190	SGDID:	S000000039	Length:	854	Species:	Saccharomyces cerevisiae

Alignment Length:	798	Identity:	150/798 - (18%)
Similarity:	276/798 - (34%)	Gaps:	224/798 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly  2818 KHLNIGESDKAERKAPESLPT---------RSLSQPNGLESYGMGRPDLEDSDSDSVASNEEAI- 2872
            :|..:.:|:.|.|.:.:..||         .||....|: ||....|....:..|::.:....|
Yeast    86 QHQELLKSNGANRDSSDLAPTLRSSSISTATSLMSMEGI-SYTNSNPSATPNMEDTLLTFSMGIL 149

  Fly  2873 ----SFYPTTYAEVLYNFTAGGPQELGLERGMLIEILRKEVGPWW------FGRIKKEETNLVED 2927
                ...|.|....|:            ::|..:.||...|.|.:      ...:|..:.::.:.
Yeast   150 PITMDCDPVTQLSQLF------------QQGAPLCILFNSVKPQFKLPVIASDDLKVCKKSIYDF 202

  Fly  2928 ILGPELGWFPKEFVRIIHCPETDNFFIAHRAAADEAAAEEATARAEEGSVPVPVAAFAEDTDVTV 2992
            |||               |.:       |.|..||.....:...|...|..|.|      .:|..
Yeast   203 ILG---------------CKK-------HFAFNDEELFTISDVFANSTSQLVKV------LEVVE 239

  Fly  2993 TTDQSNVTLIVIESPPTPSAPSVDFVAQPDHSTIIRRSA---------VRELLDTEVNYVKLLAA 3048
            |...|:.|:.     |:.|.......|:..|....::|:         ::|.:.||..||..| .
Yeast   240 TLMNSSPTIF-----PSKSKTQQIMNAENQHRHQPQQSSKKHNEYVKIIKEFVATERKYVHDL-E 298

  Fly  3049 ICDGYLPAMSKRIDIFSPNSIRLIFSNITAIYKFQRKFLEALRRGI----EQNQVAKVFLKMHKG 3109
            |.|.|...:... ::.:...:.::|.|:.....|||:||.:|....    .:.::..:|:.....
Yeast   299 ILDKYRQQLLDS-NLITSEELYMLFPNLGDAIDFQRRFLISLEINALVEPSKQRIGALFMHSKHF 362

  Fly  3110 FLCYSTYCNAYPRALIEL--ETYDRVK---DARTILENCRESQNLAELPLSAHLLAPVQRICRYP 3169
            |..|..: :....|.||.  .|..:::   ..|.|:.|        :|.|.:.|..||||:||||
Yeast   363 FKLYEPW-SIGQNAAIEFLSSTLHKMRVDESQRFIINN--------KLELQSFLYKPVQRLCRYP 418

  Fly  3170 LHLNEIIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQDTVNLALEAMRGITEAVN 3234
            |.:.|::        .|.:|.:..|              ||:         .||:..:.|..::|
Yeast   419 LLVKELL--------AESSDDNNTK--------------ELE---------AALDISKNIARSIN 452

  Fly  3235 EGKRHSETIARHQ------ASFQNFKGPPLH-----LHSARFFLQVDATRQKQNLWNSSYTLFLF 3288
            |.:|.:|   .||      ....|:||..:.     |:..:.|:   :|....:.....:.::||
Yeast   453 ENQRRTE---NHQVVKKLYGRVVNWKGYRISKFGELLYFDKVFI---STTNSSSEPEREFEVYLF 511

  Fly  3289 DNQLVYCKRDIIKR--SHFIYKGRIFLD-RCRVVNVRDGKMFGH----------TIKNSLRIYSE 3340
            :..::.....:.|:  |..|.|.:.... .....|:.|.....|          :..|::.:.|.
Yeast   512 EKIIILFSEVVTKKSASSLILKKKSSTSASISASNITDNNGSPHHSYHKRHSNSSSSNNIHLSSS 576

  Fly  3341 SRDKWYDFSFRSANRKHRFLSTLAL--------ERQFGGKALY--------------------VS 3377
            |.......|..|::......|:.:|        :....|:.:.                    :.
Yeast   577 SAAAIIHSSTNSSDNNSNNSSSSSLFKLSANEPKLDLRGRIMIMNLNQIIPQNNRSLNITWESIK 641

  Fly  3378 EMTGF--EYNYEERPGDYS---DQSDYDAQDFEL------ATGVTSGESSLPESPAKSTSRLCET 3431
            |...|  ::..||...::|   .|..:|.::.:.      :|..||       |.|||:|.:..|
Yeast   642 EQGNFLLKFKNEETRDNWSSCLQQLIHDLKNEQFKARHHSSTSTTS-------STAKSSSMMSPT 699

  Fly  3432 LPKKS-------QSRDGISSAENSQIV----------TTTSTGSLGRRHFGNWFRKPKSANCTPS 3479
            ....:       |:.|.::|..:|.:.          ||:|:.....:.....|:     |..|.
Yeast   700 TTMNTPNHHNSRQTHDSMASFSSSHMKRVSDVLPKRRTTSSSFESEIKSISENFK-----NSIPE 759

  Fly  3480 QSPTHKPSFDADATLTEA 3497
            .|...:.|::.::..|.:
Yeast   760 SSILFRISYNNNSNNTSS 777

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	DUF4802	<754..>790	CDD:292678
		SH3	2876..2942	CDD:214620	12/71 (17%)
		RhoGEF	3031..3235	CDD:279015	48/221 (22%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	26/164 (16%)
		PH	<3285..3363	CDD:278594	15/90 (17%)
CDC24	NP_009359.1	CDC24	156..242	CDD:399412	23/125 (18%)
		RhoGEF	282..453	CDD:214619	48/212 (23%)
		PH_Scd1	455..667	CDD:270066	33/217 (15%)
		PB1	779..853	CDD:214770
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001415
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.840