DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF3 and TIAM1

DIOPT Version :9

Sequence 1:	NP_612024.4	Gene:	RhoGEF3 / 38050	FlyBaseID:	FBgn0264707	Length:	3519	Species:	Drosophila melanogaster
Sequence 2:	NP_001340617.1	Gene:	TIAM1 / 7074	HGNCID:	11805	Length:	1591	Species:	Homo sapiens

Alignment Length:	1757	Identity:	362/1757 - (20%)
Similarity:	589/1757 - (33%)	Gaps:	516/1757 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly  1895 KTHALGR---------SRKSQFSRVGSAGPLIAHPNTANVGVSRFRSFDTSKSQRLNSCDSGARS 1950
            |..:|||         |.|::.:|..|:|.:| |.|             :..|.|.:|..|..:|
Human    17 KHASLGRKHTSRSLRLSHKTRRTRHASSGKVI-HRN-------------SEVSTRSSSTPSIPQS 67

  Fly  1951 DLSNDELD--NEDGGLNDFLSAGSHKFPKIGTSSVSVSPFKMQRQRSLTPDRNDSHSSTSSLRKQ 2013
             |:.:.|:  ::||.|.||.|........:|...||.:      ..|:||..:.|...|::..:.
Human    68 -LAENGLEPFSQDGTLEDFGSPIWVDRVDMGLRPVSYT------DSSVTPSVDSSIVLTAASVQS 125

  Fly  2014 RSNTPESRSLTPEERRKKGSQLSLGGARQSS-SSRSNTF-------EGRQRHDDKTPPNISRSSS 2070
            ..:|.|||....:     .:.|:.||.||.| :|...||       :.|.:..|     |.|..|
Human   126 MPDTEESRLYGDD-----ATYLAEGGRRQHSYTSNGPTFMETASFKKKRSKSAD-----IWREDS 180

  Fly  2071 SSSYSGGESHEPLNGTGSTTVTPSAGSGSTSHRRALTAKQAEQEHRIRRSRSLQLTERSPNRAHK 2135
            ........|.|.|  |.:..:..||........|.:..:.:        .|.|...:|:.
Human   181 LEFSLSDLSQEHL--TSNEEILGSAEEKDCEEARGMETRAS--------PRQLSTCQRAN----- 230

  Fly  2136 SIVNVGALVPQQRSVGVPYQQSRVPGGGVFPPTIRTSPAGAKIHVNSPAPPNSSVRVRHNETDKA 2200
               ::|.|..|:.| ||   .:....|..|....|...:......|...||.::     .||.
Human   231 ---SLGDLYAQKNS-GV---TANGGPGSKFAGYCRNLVSDIPNLANHKMPPAAA-----EETP-- 281

  Fly  2201 RSFDFDYNNFSRGGGAKGSSRTAH--TSGSGGDSGSNQNLRVERESRSFDDEICEAVLNNNNGSN 2263
                 .|:|::     ....|.:|  :.|:.....|:.|....|.:::..|      :|...||.
Human   282 -----PYSNYN-----TLPCRKSHCLSEGATNPQISHSNSMQGRRAKTTQD------VNAGEGSE 330

  Fly  2264 GSVSGLRFLQPATDSGQVSMASTSSSRLRKSNSPVSASGMEASRSPQSSGSSTNNLHQQARQSGS 2328
            .:.||:......||     :.|..|:....|.||.:......|    .||||:..  ..|||.
Human   331 FADSGIEGATTDTD-----LLSRRSNATNSSYSPTTGRAFVGS----DSGSSSTG--DAARQG-- 382

  Fly  2329 PQSYGTRLCDHELTYEMLRKSPIMNFRRGDSGDYELPVMLRNRETINSGGNSELNFMSNETRIYE 2393
                         .||        ||||      ||.:...|.|::...|::..:..|:.|
Human   383 -------------VYE--------NFRR------ELEMSTTNSESLEEAGSAHSDEQSSGT---- 416

  Fly  2394 HPTTVLKSQRSLRQSPGSRDDLNLEVAVGVGGDYIYRQPANQPRSSSVKAEFLTTATTK---SCT 2455
                        ..||| :.|:.|..|.|.         ..:..:.:|| .||.....|   |.|
Human   417 ------------LSSPG-QSDILLTAAQGT---------VRKAGALAVK-NFLVHKKNKKVESAT 458

  Fly  2456 KTLDTCDYWPRCAACQHKSVSGELKKGPSASGKPLTVLDNVQPHVTSVIVNEDNAVPGPQLRSLE 2520
            :. ....||.....|.......:.:.|...:..|        .|...|..:...|||       |
Human   459 RR-KWKHYWVSLKGCTLFFYESDGRSGIDHNSIP--------KHAVWVENSIVQAVP-------E 507

  Fly  2521 KSNPSWTFLCS---GIASICQPVQQTETD------HSTAQEAA-----EEDIV------IRKVK- 2564
            .....:.|..|   |.|.:.|...|||.:      ||....|.     :||.:      |:|::
Human   508 HPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACATAVARHHHKEDTLRLLKSEIKKLEQ 572

  Fly  2565 ----PNKSRRTGRSRSLPSVMLRRLYLAGMPKPGSNEMGTTSDCAASLSQQIKAVSSPTLSEPCL 2625
                ..|.::.|..: |.||            ..|.:..|..|......|.::..........|.
Human   573 KIDMDEKMKKMGEMQ-LSSV------------TDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCY 624

  Fly  2626 EEQPQKG-----TESTDVANTGSSAGSGGLGVLQKFKRTLNNFNNKNQLHISPLSPAAVGNNAPK 2685
            ....|.|     ......|:..:....|.||:.     ::::|:        .|..|..|....:
Human   625 LASLQGGELPNPKRLLAFASRPTKVAMGRLGIF-----SVSSFH--------ALVAARTGETGVR 676

  Fly  2686 TKTSPTTTTASIAPMITTTTASDPGSEAGDTSSGKYRFGPLIW----RSSKERRKTKYNR----- 2741
            .:|...:.:|                     |..:.||..| |    .|.|::.:...|:
Human   677 RRTQAMSRSA---------------------SKRRSRFSSL-WGLDTTSKKKQGRPSINQVFGEG 719

  Fly  2742 RDKCNSGDSGIQIELEQDEQYSRATMA-------------VSRQDEPRAFALTNGSCGV------ 2787
            .:.......||..::..|.:..:..:.             |.....|..|.|.|....:
Human   720 TEAVKKSLEGIFDDIVPDGKREKEVVLPNVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPG 784

  Fly  2788 --ALSKMRAIRRTNSAKASS--ILGPFIVKTKIAKHLNIGESDKAE--RKAPESLP--TRSL--- 2841
              |...:..|.:|:....|:  :...|:::.|:..::...|.|..|  .|..|..|  |:|:
Human   785 DTARDTLELICKTHQLDHSAHYLRLKFLIENKMQLYVPQPEEDIYELLYKEIEICPKVTQSIHIE 849

  Fly  2842 SQPNGLESYGMGRPDLEDSDSDSVASNEEAI-SFYPTTYAEVLYNFTAGGPQELGLERG-MLIEI 2904
            ......::||.....:|          |:.| ..|..:..|      .|...:.||:.| .::||
Human   850 KSDTAADTYGFSLSSVE----------EDGIRRLYVNSVKE------TGLASKKGLKAGDEILEI 898

  Fly  2905 LRKEVGPWWFGRIKKEETNLVEDILG-PELG----WFP--KEFVRIIHCP-----------ETDN 2951
            ..:.....        .:::::|.|. |.||    .:|  :|.|.::..|           |:..
Human   899 NNRAADAL--------NSSMLKDFLSQPSLGLLVRTYPELEEGVELLESPPHRVDGPADLGESPL 955

  Fly  2952 FFI----AHRAAADEAAAEEATARAEEG-------------SVPVPVAAFAEDTDVTVTTDQSNV 2999
            .|:    .|...:::.::.|......||             .....||||.........:|||
Human   956 AFLTSNPGHSLCSEQGSSAETAPEETEGPDLESSDETDHSSKSTEQVAAFCRSLHEMNPSDQS-- 1018

  Fly  3000 TLIVIESPPTPSAPSVDFVAQPDHSTIIRRSAVRELLDTEVNYVKLLAAICDGYLPAMSKRIDIF 3064
                 .||...:.|.:..:.|...:..:|: .:.|||:||..|||.|..:.:.||..:.|. ...
Human  1019 -----PSPQDSTGPQLATMRQLSDADKLRK-VICELLETERTYVKDLNCLMERYLKPLQKE-TFL 1076

  Fly  3065 SPNSIRLIFSNITAIYKFQRKFLEALRRGI----------EQNQVAKVFLKMHKGFL-------C 3112
            :.:.:.::|.|:|.:.:||.:||:.|..|:          :.:|..||...:...||       .
Human  1077 TQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKL 1141

  Fly  3113 YSTYCNAY---PRALIELETYDRVKDARTILENCRESQNLAELPLSAHLLAPVQRICRYPLHLNE 3174
            ||.:|.::   |:.|::.:| |....|....:|.::..:..   |.::|:.|:|||.:|||.|.|
Human  1142 YSAFCASHTKVPKVLVKAKT-DTAFKAFLDAQNPKQQHSST---LESYLIKPIQRILKYPLLLRE 1202

  Fly  3175 IIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQDTVNLALEAMRGITEAVNE-GKR 3238
            :.  ||                   ||.|..:...||:         |::.|..:...:|| .|.
Human  1203 LF--AL-------------------TDAESEEHYHLDV---------AIKTMNKVASHINEMQKI 1237

  Fly  3239 HSE-------TIAR---HQASFQNFKGPPLHLHSARFFLQVDATRQKQNLWNSSYTL--FLFDNQ 3291
            |.|       .||.   .:....:.....|.||:...:|...|:..|   |.....|  |:|...
Human  1238 HEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWLNPPASLGK---WKKEPELAAFVFKTA 1299

  Fly  3292 LVYCKRDIIKRSHFIYKGRIFLDRCRVVNVRDG-----KMFGHTIKNSLRIYSESRDKWYDFSFR 3351
            :|           .:||              ||     |:.|   .:.|.||.:    |..|.||
Human  1300 VV-----------LVYK--------------DGSKQKKKLVG---SHRLSIYED----WDPFRFR 1332

  Fly  3352 SANRKHRFLSTLALE----RQFGGKALYVSEMTGFEYNYEERPG---DYSDQSDYDAQDFELATG 3409
                  ..:.|.||:    .....:|..|.|:...:...|.||.   .....|....:||..|.
Human  1333 ------HMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAV- 1390

  Fly  3410 VTSGESSLPESPAKSTSRLCETLPKKSQ--------------SRDGISSAENSQIVTTTSTGSLG 3460
                .|.|.:...:...: .|:||...|              :|..:|.|      .:..:.|||
Human  1391 ----HSILRDKHRRQLLK-TESLPSSQQYVPFGGKRLCALKGARPAMSRA------VSAPSKSLG 1444

  Fly  3461 RR 3462
            ||
Human  1445 RR 1446

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	DUF4802	<754..>790	CDD:292678
		SH3	2876..2942	CDD:214620	14/73 (19%)
		RhoGEF	3031..3235	CDD:279015	55/223 (25%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	30/153 (20%)
		PH	<3285..3363	CDD:278594	17/84 (20%)
TIAM1	NP_001340617.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..78		19/75 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	298..379		22/97 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	393..422		8/45 (18%)
		PH1_Tiam1_2	432..558	CDD:269937	30/151 (20%)
		TIAM1_RBD	766..841	CDD:176413	15/74 (20%)
		PDZ_signaling	846..925	CDD:238492	18/102 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..1034		17/101 (17%)
		RhoGEF	1044..1233	CDD:214619	55/223 (25%)
		PH2_Tiam1_2	1235..1406	CDD:269957	44/217 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1456..1482
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810