Protein Alignment RhoGEF3 and PLEKHG1

DIOPT Version :10

• Sequence 1: NP_612024.4
• Gene: RhoGEF3 / 38050
• FlyBaseID: FBgn0264707
• Length: 3519
• Species: Drosophila melanogaster
• Sequence 2: NP_001316727.1
• Gene: PLEKHG1 / 57480
• HGNCID: 20884
• Length: 1444
• Species: Homo sapiens
• Alignment Length: 717
• Identity: 171/717 - (23%)
• Similarity: 262/717 - (36%)
• Gaps: 208/717 - (29%)

  Fly  2769 VSRQDEPRAFALTNGSCGVALSKMRAIRRTNSAKASSILGPFIVKTKIAKHLNIGESDKAERKAP 2833
            |.|:.:..| |.||...|.:::....:|....:..:.:..|   ||            .|.|..|
Human     6 VGRKRQETA-AWTNRVLGQSITDGHQLRLEVPSAVTKVPHP---KT------------TARRSCP 54

  Fly  2834 ESLPTRSLSQPNGLESYGMGRPDLEDSDSDSVASNEEAISFYPTTYAEVLYNFTAGGPQELGLER 2898
            :.|.|..||..:...|:|        |.|.|.:|.:...||              |....|....
Human    55 QELKTMELSDSDRPVSFG--------STSSSASSRDSHGSF--------------GSRMTLVSNS 97

  Fly  2899 GMLIEILRKEVGPWWFGRIKKEETNLVEDILGPELGWFPKEFVRIIHCPETDNFFIAHRAAADEA 2963
            .|.:....|||     |.||.|        |.|...:...|..|       ||          .|
Human    98 HMGLFNQDKEV-----GAIKLE--------LIPARPFSSSELQR-------DN----------PA 132

  Fly  2964 AAEEATARAEEGSVPVPVAAFAEDTDVTVTTDQSNVTLIVIESPPTPSAPSVDFVAQPDHSTIIR 3028
            ..::   .|:|||...|.|.:..|::....|        :.:|..:|....||.|
Human   133 TGQQ---NADEGSERPPRAQWRVDSNGAPKT--------IADSATSPKLLYVDRV---------- 176

  Fly  3029 RSAVRELLDTEVNYVKLLAAICDGYLPAMSKRIDI-FSPNSIRLIFSNITAIYKFQRKFLEALRR 3092
               |:|:|:||..||:.|.:|.:.||..:..:..: ........:|.||..||.|..:.|:.| .
Human   177 ---VQEILETERTYVQDLKSIVEDYLDCIRDQTKLPLGTEERSALFGNIQDIYHFNSELLQDL-E 237

  Fly  3093 GIEQNQV--AKVFLKMHKGFLCYSTYCNAYPRALIELETYDRVKDARTILENCRESQNLAE---- 3151
            ..|.:.|  |:.|:...:.|..|:.||..|||::             .:|..|..::.||:
Human   238 NCENDPVAIAECFVSKSEEFHIYTQYCTNYPRSV-------------AVLTECMRNKILAKFFRE 289

  Fly  3152 --------LPLSAHLLAPVQRICRYPLHLNEIIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVT 3208
                    |||.::||.|||||.:|.|.|:| |::.|:|      ||:|
Human   290 RQETLKHSLPLGSYLLKPVQRILKYHLLLHE-IENHLDK------DTEG---------------- 331

  Fly  3209 ELDIPDSQDTVNLALEAMRGITEAVNEGKR---HSETIARHQASFQNFKGPPLHLHSARFFLQVD 3270
                   .|.|..|::.|:.:...:|:.||   |:..:...|:...|:|||.|..:..   |.::
Human   332 -------YDVVLDAIDTMQRVAWHINDMKRKHEHAVRLQEIQSLLTNWKGPDLTSYGE---LVLE 386

  Fly  3271 ATRQKQNLWNSSYTLFLFDNQLVYCKRDIIKRSHFIYKGRIFLDRCRVVNV-------------R 3322
            .|.:.|...|.. ||||||..|:..|:   :...|.||..|......:|.|             :
Human   387 GTFRIQRAKNER-TLFLFDKLLLITKK---RDDTFTYKAHILCGNLMLVEVIPKEPLSFSVFHYK 447

  Fly  3323 DGKMFGHTIKNSLRIYSESRDK--WYDFSFR----------SANRKHRFLSTLALER-------Q 3368
            :.|: .||::     ....:||  |.....|          .|..|...|...|:..       :
Human   448 NPKL-QHTVQ-----AKSQQDKRLWVLHLKRLILENHAAKIPAKAKQAILEMDAIHHPGFCYSPE 506

  Fly  3369 FGGKALYVSEMTGFEYNYEERPGDYSDQSDYDAQDFELATGV--TSGESSLPE------SPAKST 3425
            .|.|||:.|:.....|.. .|..:.|.:|....:..|.|..:  .|.|...|:      |||:..
Human   507 GGTKALFGSKEGSAPYRL-RRKSEPSSRSHKVLKTSETAQDIQKVSREEGSPQLSSARPSPAQRN 570

  Fly  3426 SR 3427
            |:
Human   571 SQ 572

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	SH3	2876..2942	CDD:214620	13/65 (20%)
		RhoGEF	3028..3234	CDD:238091	58/220 (26%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	36/164 (22%)
PLEKHG1	NP_001316727.1	RhoGEF	176..350	CDD:459876	59/230 (26%)
		PH_PLEKHG1_G2_G3	332..477	CDD:270063	39/157 (25%)